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Neuroprotective effects of Shaoyao Gancao decoction against excitatory damage in PC12 cells based on the Src-NR2-nNOS pathway
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作者 Xiaoxu Fan Hongyan Ma +6 位作者 Tiantian Zhou Min Fu Zhiyuan Qiao Yingtong Feng Zhen Wang yiwei shen Jingxia Wang 《Journal of Traditional Chinese Medical Sciences》 CAS 2024年第3期293-302,共10页
Objective:To explore the neuroprotective effects of the Shaoyao Gancao decoction(SGD)against excitatory damage in PC12 cells and the role of the Src-NR2-nNOS pathway mediation by SGD in regulatingγ-aminobutyric acid(... Objective:To explore the neuroprotective effects of the Shaoyao Gancao decoction(SGD)against excitatory damage in PC12 cells and the role of the Src-NR2-nNOS pathway mediation by SGD in regulatingγ-aminobutyric acid(GABA)-glutamate(Glu)homeostasis.Methods: N-Methyl-d-aspartic acid(NMDA)was used to establish a PC12 cell excitability injury model.To investigate the neuroprotective effect of SGD,a cell counting kit-8(CCK-8)assay was used to determine PC12 cell viability,Annexin V/Propidium Iodide(Annexin V/PI)double staining was used to determine PC12 cell apoptosis,and Ca^(2+)concentration was observed using laser confocal microscopy.GABA receptor agonists and antagonists were used to analyze the neuroprotective interactions betweenγ-aminobutyric acid(GABA)and NMDA receptors.Additionally,molecular biology techniques were used to determine mRNA and protein expression in the Src-NR2-nNOS pathway.We analyzed the correlations between the regulatory sites of GABA and NMDA interactions,excitatory neurotoxicity,and brain damage at the molecular level.Results: NMDA excitotoxic injury manifested as a significant decrease in cell activity,increased apoptosis and caspase-3 protein expression,and a significant increase in intracellular Ca^(2+)concentration.Administration of SGD,a GABAA receptor agonist(muscimol),or a GABAB receptor agonist(baclofen)decreased intracellular Ca^(2+)concentrations,attenuated apoptosis,and reversed NMDA-induced upregulation of caspase-3,Src,NMDAR2A,NMDAR2B,and nNOS.Unexpectedly,a GABA_(A)receptor antagonist(bicuculline)and a GABA_(B)receptor antagonist(saclofen)failed to significantly increase excitatory neurotoxicity.Conclusions: Taken together,these results not only provide an experimental basis for SGD administration in the clinical treatment of central nervous system injury diseases,but also suggest that the Src-NR2A-nNOS pathway may be a valuable target in excitotoxicity treatment. 展开更多
关键词 Shaoyao Gancao decoction PC12 N-Methyl-D-aspartic acid(NMDA) γ-aminobutyric acid(GABA) SRC NNOS
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HOXB8 contributed to oxaliplatin chemo-resistance in colon cancercells by activating STAT3
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作者 LIANLI NI YUN YU +5 位作者 HAN LIN WEISHAN ZHUGE LU TAO yiwei shen RI CUI SHAOTANG LI 《BIOCELL》 SCIE 2023年第10期2245-2254,共10页
Background:Homeobox B8(HOXB8),a member of HOX family,plays a key role in the development of colorectal cancer(CRC).However,the function of HOXB8 in oxaliplatin(OXA)resistance in CRC is still unclear.This study investi... Background:Homeobox B8(HOXB8),a member of HOX family,plays a key role in the development of colorectal cancer(CRC).However,the function of HOXB8 in oxaliplatin(OXA)resistance in CRC is still unclear.This study investigated the role and precise molecular mechanism of HOXB8 in OXA-resistant CRC cells.Methods:The cell viability was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide(MTT)assay,and the colony forming ability was determined by colony formation assay.The silencing RNA(siRNA)approach was used to knockdown HOXB8 in CRC cells while the lentiviral transfection system was used to establish stable HOXB8 overexpressing CRC cells.The protein and mRNA levels were evaluated by western blot and real-time reverse transcription-polymerase chain reaction.Results:HOXB8 expression was upregulated in OXA-resistant HCT116 cells(HCT116/OXA)compared to its level in the parent HCT116 cells.Knockdown of HOXB8 significantly inhibited CRC cell growth by suppressing the signal transducer and activator of transcription 3(STAT3)pathway.HOXB8 knockdown also potentiated cytotoxicity of OXA in CRC cells.Inversely,HOXB8 overexpression attenuated OXAinduced growth inhibition of HCT116 cells and RKO cells by activating STAT3 signaling.HOXB8 knockdown effectively inhibited HCT116/OXA cell viability regardless of OXA treatment by suppressing STAT3 signaling.Conclusions:These results shed light on the important functions of HOXB8 in OXA-resistant CRC and suggested that targeting HOXB8 might be an effective therapeutic strategy for select OXA-resistant CRC patients. 展开更多
关键词 HOXB8 DRUG-RESISTANCE Colorectal cancer STAT3 OXALIPLATIN
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Repression of interferon regulatory factor-4(IRF4)hyperactivation restricts murine lupus 被引量:1
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作者 Shijun He Huihua Ding +7 位作者 Li Chen yiwei shen Yuting Liu Fenghua Zhu Xiaoqian Yang Nan shen Zemin Lin Jianping Zuo 《Signal Transduction and Targeted Therapy》 SCIE CSCD 2023年第6期2475-2477,共3页
Dear Editor,Hyperactivation of the type I interferon(IFN)signature has been observed in several systemic autoimmune conditions,and the type I IFN receptor antagonist Saphnelo(anifrolumab-fnia)has been approved in 2021... Dear Editor,Hyperactivation of the type I interferon(IFN)signature has been observed in several systemic autoimmune conditions,and the type I IFN receptor antagonist Saphnelo(anifrolumab-fnia)has been approved in 2021 by the Food and Drug Administration(FDA)in the US.The launch of Saphnelo marked the only new treatment approved for systemic lupus erythematosus(SLE)in more than 10 years. 展开更多
关键词 LUPUS INTERFERON ACTIVATION
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甲醇微生物转化最新进展
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作者 蔡鹏 吴晓燕 +3 位作者 解林峰 申益维 高琳惠 周雍进 《中国科学:化学》 CAS 2024年第11期2199-2218,共20页
甲醇生物转化是CO_(2)转化和可再生能源利用的有效方式,是低碳生物转化研究领域的热点.然而微生物在甲醇代谢模式下面临甲醇生物毒性大、甲醇耐受性低、代谢适配性差等诸多挑战,导致了较低的生物合成效率.本文综述了甲醇微生物转化近年... 甲醇生物转化是CO_(2)转化和可再生能源利用的有效方式,是低碳生物转化研究领域的热点.然而微生物在甲醇代谢模式下面临甲醇生物毒性大、甲醇耐受性低、代谢适配性差等诸多挑战,导致了较低的生物合成效率.本文综述了甲醇微生物转化近年来的研究现状,重点总结了天然甲基营养型菌株与人工甲基营养型菌株的代谢途径改造与设计,甲醇代谢过程的理性改造与进化工程策略,甲醇发酵工艺现状等内容.最后,探讨了当前甲醇生物转化面临的挑战,并为甲醇生物转化的产业化提出了可行的发展方向. 展开更多
关键词 甲醇生物转化 甲醇脱氢酶 甲醛同化 甲醇细胞工厂 适应性进化
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