This paper establishes a short-term prediction model of weekly retail prices for eggs based on chaotic neural network with the weekly retail prices of eggs from January 2008 to December 2012 in China.In the process of...This paper establishes a short-term prediction model of weekly retail prices for eggs based on chaotic neural network with the weekly retail prices of eggs from January 2008 to December 2012 in China.In the process of determining the structure of the chaotic neural network,the number of input layer nodes of the network is calculated by reconstructing phase space and computing its saturated embedding dimension,and then the number of hidden layer nodes is estimated by trial and error.Finally,this model is applied to predict the retail prices of eggs and compared with ARIMA.The result shows that the chaotic neural network has better nonlinear fitting ability and higher precision in the prediction of weekly retail price of eggs.The empirical result also shows that the chaotic neural network can be widely used in the field of short-term prediction of agricultural prices.展开更多
The human interferon α 2b(hIFN-α2b) gene was cloned into binary vector pBI121 to obtain plant expression vector pBIFN. The recombinant plasmid pBIFN was transferred into Agrobacterium tumefaciens strain LBA4404. The...The human interferon α 2b(hIFN-α2b) gene was cloned into binary vector pBI121 to obtain plant expression vector pBIFN. The recombinant plasmid pBIFN was transferred into Agrobacterium tumefaciens strain LBA4404. Then the hIFN-α2b gene was introduced into ginseng callus cells via Agrobacterium-mediated transformation and the ginseng cell line carrying hIFN-α2b gene was selected on G418-containing medium. The presence of target gene in transformed cells was confirmed by PCR and RT-PCR. The results indicate that hIFN-α2b gene has been integrated into the ginseng cells’ genome, with transcription products. hIFN-α2b expressed by the transgenic ginseng cells was detected by Western blot. It was shown that a specific protein band at 19000 could be observed. Cytopathic effect(CPE) inhibition assay using the WISH-VSV system shows that the mean antiviral activity of expressed hIFN-α2b was 6.0×104 IU/mL.展开更多
基金financially supported by the National KeyTechnology R&D Program during the 12th Five-Year Plan period(2012BAH20B04)the 948 Program of Ministry of Agriculture,China(2013-Z1)
文摘This paper establishes a short-term prediction model of weekly retail prices for eggs based on chaotic neural network with the weekly retail prices of eggs from January 2008 to December 2012 in China.In the process of determining the structure of the chaotic neural network,the number of input layer nodes of the network is calculated by reconstructing phase space and computing its saturated embedding dimension,and then the number of hidden layer nodes is estimated by trial and error.Finally,this model is applied to predict the retail prices of eggs and compared with ARIMA.The result shows that the chaotic neural network has better nonlinear fitting ability and higher precision in the prediction of weekly retail price of eggs.The empirical result also shows that the chaotic neural network can be widely used in the field of short-term prediction of agricultural prices.
基金Supported by the Science and Technology Development Planning Foundation of Jilin Province of China(No.20030405)
文摘The human interferon α 2b(hIFN-α2b) gene was cloned into binary vector pBI121 to obtain plant expression vector pBIFN. The recombinant plasmid pBIFN was transferred into Agrobacterium tumefaciens strain LBA4404. Then the hIFN-α2b gene was introduced into ginseng callus cells via Agrobacterium-mediated transformation and the ginseng cell line carrying hIFN-α2b gene was selected on G418-containing medium. The presence of target gene in transformed cells was confirmed by PCR and RT-PCR. The results indicate that hIFN-α2b gene has been integrated into the ginseng cells’ genome, with transcription products. hIFN-α2b expressed by the transgenic ginseng cells was detected by Western blot. It was shown that a specific protein band at 19000 could be observed. Cytopathic effect(CPE) inhibition assay using the WISH-VSV system shows that the mean antiviral activity of expressed hIFN-α2b was 6.0×104 IU/mL.
