Objective:Resveratrol polymer micelles with tissue adhesion were prepared and the content of resveratrol in the micelles was determined by HPLC.Method:The micelle adhesion experiment was carried out by polylysine orif...Objective:Resveratrol polymer micelles with tissue adhesion were prepared and the content of resveratrol in the micelles was determined by HPLC.Method:The micelle adhesion experiment was carried out by polylysine orifice plate experiment and small animal fluorescence imaging method,and the micelle prescription was optimized to obtain resveratrol micelles with good adhesion.The separation was performed on a Shiseido SPOLAR C18 column(150 mm×4.6 mm,5μm)with methanol-water(42:58)as the mobile phase.The flow rate was 1.0 mL·min^(-1),the detection wavelength was 305 nm,the column temperature was 35℃,and the injection volume was 10μL.Results:Resveratrol micelles prepared with F127 alone had the best adhesion.The peak area and concentration of resveratrol had a good linear relationship in the concentration range of 10~200μg/mL(r=0.9996).The specificity,precision,recovery and stability all met the methodological requirements.Conclusion:In this experiment,resveratrol micelles with tissue adhesion were successfully prepared,and a method for the determination of resveratrol content in micelles was established.The method is accurate,rapid and simple.展开更多
目的探讨应用第八版TNM分期对肿瘤浸润性直径进行分期的临床意义。方法回顾性分析具有贴壁样成分的≤5 cm N0M0肺腺癌切除病例200例,按照新分期进行病理分期后的变化及临床意义。结果以浸润性直径进行分期后,有54.5%(109/200)的肿瘤发生...目的探讨应用第八版TNM分期对肿瘤浸润性直径进行分期的临床意义。方法回顾性分析具有贴壁样成分的≤5 cm N0M0肺腺癌切除病例200例,按照新分期进行病理分期后的变化及临床意义。结果以浸润性直径进行分期后,有54.5%(109/200)的肿瘤发生了T分期的改变;39.5%的肿瘤发生了IA期下调改变,11%(22/200)的病例由IB期变成IA期,4%(8/200)的病例由II期变成了I期,占整个II期病例的40%(8/20)。浸润性直径大小对患者生存期具有更好的预测作用,且浸润性肿瘤大小与肺部CT实性区大小显示更好的正相关性。结论直径1~2 cm的肿瘤中,存在非浸润性成分的概率大于其他肿瘤。新分期与影像学联系更密切,兼顾了肿瘤的病理学和影像学特性,浸润性肿瘤大小对生存期的预测明显好于肿瘤总大小。展开更多
基金High Level Talent Program of Hainan Natural Science Foundation (821RC569)National Natural Science Foundation-Regional Science Foundation Project (82060642)+3 种基金Hainan Provincial Association of Science and Technology Youth Science and Technology Talent Innovation Program Project (QCXM202029)Hainan Province Higher Education Science Research Project (Hnky2020-35)National Natural Science Foundation-Youth Science Foundation Project (81502998)Hainan Medical College 2022 College Student Innovation and Entrepreneurship Training Program Project (X202211810094)。
文摘Objective:Resveratrol polymer micelles with tissue adhesion were prepared and the content of resveratrol in the micelles was determined by HPLC.Method:The micelle adhesion experiment was carried out by polylysine orifice plate experiment and small animal fluorescence imaging method,and the micelle prescription was optimized to obtain resveratrol micelles with good adhesion.The separation was performed on a Shiseido SPOLAR C18 column(150 mm×4.6 mm,5μm)with methanol-water(42:58)as the mobile phase.The flow rate was 1.0 mL·min^(-1),the detection wavelength was 305 nm,the column temperature was 35℃,and the injection volume was 10μL.Results:Resveratrol micelles prepared with F127 alone had the best adhesion.The peak area and concentration of resveratrol had a good linear relationship in the concentration range of 10~200μg/mL(r=0.9996).The specificity,precision,recovery and stability all met the methodological requirements.Conclusion:In this experiment,resveratrol micelles with tissue adhesion were successfully prepared,and a method for the determination of resveratrol content in micelles was established.The method is accurate,rapid and simple.
文摘目的探讨应用第八版TNM分期对肿瘤浸润性直径进行分期的临床意义。方法回顾性分析具有贴壁样成分的≤5 cm N0M0肺腺癌切除病例200例,按照新分期进行病理分期后的变化及临床意义。结果以浸润性直径进行分期后,有54.5%(109/200)的肿瘤发生了T分期的改变;39.5%的肿瘤发生了IA期下调改变,11%(22/200)的病例由IB期变成IA期,4%(8/200)的病例由II期变成了I期,占整个II期病例的40%(8/20)。浸润性直径大小对患者生存期具有更好的预测作用,且浸润性肿瘤大小与肺部CT实性区大小显示更好的正相关性。结论直径1~2 cm的肿瘤中,存在非浸润性成分的概率大于其他肿瘤。新分期与影像学联系更密切,兼顾了肿瘤的病理学和影像学特性,浸润性肿瘤大小对生存期的预测明显好于肿瘤总大小。
