Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collect...Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.展开更多
目的建立微波消解-电感耦合等离子体质谱法(inductivelycoupledplasma-massspectrometry,ICP-MS)同时测定小麦中35种元素(Li、Be、V、Cr、Mn、Co、Ni、Cu、As、Se、Y、Mo、Ag、Cd、Sb、Ba、La、Ce、Pr、Nd、Sm、Eu、Gd、Tb、Dy、Ho、Er...目的建立微波消解-电感耦合等离子体质谱法(inductivelycoupledplasma-massspectrometry,ICP-MS)同时测定小麦中35种元素(Li、Be、V、Cr、Mn、Co、Ni、Cu、As、Se、Y、Mo、Ag、Cd、Sb、Ba、La、Ce、Pr、Nd、Sm、Eu、Gd、Tb、Dy、Ho、Er、Tm、Yb、Lu、Hg、Tl、Pb、Th、U)的分析方法。方法经微波消解处理后,用电感耦合等离子体质谱法对样品进行测定,以Sc、Rh、In、Bi为内标元素校正基体效应和信号漂移。结果各元素均呈良好的线性关系,相关系数在0.9996~1.0000之间。小麦中35种元素检测方法的检出限为0.0001~0.0030mg/kg,加标回收率在89.0%~115.5%之间,相对标准偏差(relative standard deviation, RSD)在0.36%~4.90%之间。分别采用一级标准物质河南小麦(GBW 10046)和四川大米(GBW10044)来验证方法的精密度和准确度,结果均在标准值范围内。对60份小麦样品进行测定,所得结果满意。结论该方法用于小麦中多元素的测定,操作简便、分析速度快、灵敏度高,为小麦的质量控制和营养评价提供依据。展开更多
文摘Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.
文摘目的建立微波消解-电感耦合等离子体质谱法(inductivelycoupledplasma-massspectrometry,ICP-MS)同时测定小麦中35种元素(Li、Be、V、Cr、Mn、Co、Ni、Cu、As、Se、Y、Mo、Ag、Cd、Sb、Ba、La、Ce、Pr、Nd、Sm、Eu、Gd、Tb、Dy、Ho、Er、Tm、Yb、Lu、Hg、Tl、Pb、Th、U)的分析方法。方法经微波消解处理后,用电感耦合等离子体质谱法对样品进行测定,以Sc、Rh、In、Bi为内标元素校正基体效应和信号漂移。结果各元素均呈良好的线性关系,相关系数在0.9996~1.0000之间。小麦中35种元素检测方法的检出限为0.0001~0.0030mg/kg,加标回收率在89.0%~115.5%之间,相对标准偏差(relative standard deviation, RSD)在0.36%~4.90%之间。分别采用一级标准物质河南小麦(GBW 10046)和四川大米(GBW10044)来验证方法的精密度和准确度,结果均在标准值范围内。对60份小麦样品进行测定,所得结果满意。结论该方法用于小麦中多元素的测定,操作简便、分析速度快、灵敏度高,为小麦的质量控制和营养评价提供依据。
