Background:Preeclampsia(PE),characterized by hypertension and proteinuria,leads to serious maternal and infant complications.Uridine-cytidine kinase 2(UCK2)belongs to the UCK family,a class of enzymes that catalyzes t...Background:Preeclampsia(PE),characterized by hypertension and proteinuria,leads to serious maternal and infant complications.Uridine-cytidine kinase 2(UCK2)belongs to the UCK family,a class of enzymes that catalyzes the conversion of uridine and cytidine to monophosphate form.However,the role of UCK2 in PE has not been reported.Methods:The expression of UCK2 was detected in the placenta of PE patients and N(ω)-nitro-L-arginine methyl esterinduced PE mouse model.Through forced up-regulation or down-regulation of UCK2 in vitro,we examined the effects of UCK2 on the proliferation,apoptosis,migration,and invasion of trophoblast cells.Stattic,the inhibitor of STAT3 pathway,was used to investigate whether the STAT3 pathway mediates the biological function of UCK2 in trophoblast cells.Results:The present study found that UCK2 showed low expression in the placenta of PE patients and PE mouse model.MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)and flow cytometry assays verified that up-regulation of UCK2 promoted the proliferation of trophoblast cells,while the silence of UCK2 suppressed cell proliferation.Besides,flow cytometry and TdT-mediated dUTP Nick-End Labeling assays demonstrated that knockdown of UCK2 resulted in apoptosis of trophoblast cells.The wound healing and transwell assays showed that the migration and invasion activities of the trophoblast cells were facilitated by the overexpression of UCK2 and were blocked by the silence of UCK2.Furthermore,the expression of phosphorylated STAT3 was increased with the upregulation of UCK2 and decreased with the inhibition of UCK2.When the STAT3 pathway was blocked by its inhibitor stattic,the promotion effects of UCK2 on trophoblast cells were suppressed.Conclusion:UCK2 promotes the proliferation,migration,and invasion of trophoblast cells,and these effects may be partly mediated by the activation of the STAT3 pathway.展开更多
The aerobic oxidation of glycerol provides an economically viable route to glyceraldehyde, dihydroxyacetone and glyceric acid with versatile applications, for which monometallic Pt, Au and Pd and bimetallic Au-Pt, Au-...The aerobic oxidation of glycerol provides an economically viable route to glyceraldehyde, dihydroxyacetone and glyceric acid with versatile applications, for which monometallic Pt, Au and Pd and bimetallic Au-Pt, Au- Pd and Pt-Pd catalysts on TiO2 were examined under base-free conditions. Pt exhibited a superior activity relative to Pd, and Au-Pd and Pt-Pd while Au was essentially inactive. The presence of Au on the Au-Pt/TiO2 catalysts led to their higher activities （normalized per Pt atom） in a wide range of Au/Pt atomic ratios （i.e. 1/3-7/1 ）, and the one with the Au/Pt ratio of 3/1 exhibited the highest activity. Such promoting effect is ascribed to the increased electron density on Pt via the electron transfer from Au to Pt, as characterized by the temperature-programmed desorption of CO and infra-red spectroscopy for CO adsorption. Meanwhile, the presence of Au on Au-Pt/TiO2, most like due to the observed electron transfer, changed the product selectivity, and facilitated the oxidation of the secondary hydroxyl groups in glycerol, leading to the favorable formation of dihydroxyacetone over glyceraldehyde and glyceric acid that were derived from the oxidation of the primary hydroxyl groups. The synergetic effect between Au and Pt demonstrates the feasibility in the efficient oxidation of glycerol to the targeted products, for example, by rational tuning of the electronic properties of metal catalysts.展开更多
In order to measure the residual stress components in an elastic thin plate, the hole-drilling strain-gage method has been used. This method enables to determine the relation between the magnitudes and directions of t...In order to measure the residual stress components in an elastic thin plate, the hole-drilling strain-gage method has been used. This method enables to determine the relation between the magnitudes and directions of the principal stresses and the strain relaxation about the hole. In the existing analytical models based on stress field, the formulations associated with the hole-drilling method are based on the assumption of an infinite plate, this may cause some errors for a finite plate and it’s difficult to validate these solutions by FE methods. Furthermore, in the composite, the displacement field is continuous but the stress field is not necessarily continuous, the displacement field based method has to be used. In the present paper an analyt-ical model based on a displacement field described by a function with coefficients to determine for a finite round thin plate is presented. The coefficients used in the displacement field are independent on the three residual stress components, and they are determined by minimization of the internal strain energy during the hole-drilling process. Once the coefficients in the dis-placement field are determined, three strains measured in three radial directions are utilized to determine the three residual stress components. The proposed analytical model can be also adapted to infinite plate by assuming that the diameter of the round plate tends to infinite.展开更多
This study was conducted to investigate the gene expression in fructose-fed rat skeletal muscle by cDNA chip which could provide support to elucidate the molecular mechanisms underlying insulin resistance. The rats we...This study was conducted to investigate the gene expression in fructose-fed rat skeletal muscle by cDNA chip which could provide support to elucidate the molecular mechanisms underlying insulin resistance. The rats were divided into two groups, one of which was normal control and the other was fed with fructose-rich diet. The mRNA was isolated and purified from the skeletal muscle of two groups. The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and Cy5-dUTP separately to prepare hybridization probes. The mixed probes were hybridized to cDNA microarray. The microarray was scanned, analyzed and repeated for two times. Among the total 4 096 tested genes, 140 genes were differently expressed, 62 up-regulated,78 down-regulated, the expression of Ptprd and Gilz and multiple genes of oxidative metabolism is associated in insulin resistance. The differential expression of gene may be related to the pathogenesis of insulin resistance.展开更多
Fulminant myocarditis is an acute diffuse inflammatory disease of myocardium.It is characterized by acute onset,rapid progress and high risk of death.Its pathogenesis involves excessive immune activation of the innate...Fulminant myocarditis is an acute diffuse inflammatory disease of myocardium.It is characterized by acute onset,rapid progress and high risk of death.Its pathogenesis involves excessive immune activation of the innate immune system and formation of inflammatory storm.According to China’s practical experience,the adoption of the“life support-based comprehensive treatment regimen”(with mechanical circulation support and immunomodulation therapy as the core)can significantly improve the survival rate and long-term prognosis.Special emphasis is placed on very early identification,very early diagnosis,very early prediction and very early treatment.展开更多
To improve the standard screening, diagnosis, and treatment of hypertension in patients in China;realize the standardization of clinical practice of hypertension;and improve the prevention and control level of hyperte...To improve the standard screening, diagnosis, and treatment of hypertension in patients in China;realize the standardization of clinical practice of hypertension;and improve the prevention and control level of hypertension in China, it is both important and necessary to develop a clinical practice guideline for hypertension according to a recognized methodology. Jointly sponsored by the National Center for Cardiovascular Diseases, Chinese Medical Doctor Association, Hypertension Committee of the Chinese Medical Doctor Association, Chinese Society of Cardiology, and Hypertension Committee of Cross-Straits Medicine Exchange Association, the “Chinese Clinical Practice Guidelines of Hypertension” was proposed. Research Unit of Evidence-Based Evaluation and Guidelines, Chinese Academy of Medical Sciences, Guideline and Standards Research Centre of Chinese Medical Association Publishing House, Lanzhou University Institute of Health Data Science, and Lanzhou University GRADE Center will provide methodological support for the guidelines.展开更多
The carotenoid in sweet potato has a high health value for the human body,and Harvest Plus has also carried out the breeding to improve the nutritional quality of sweet potatoes in order to address the health problems...The carotenoid in sweet potato has a high health value for the human body,and Harvest Plus has also carried out the breeding to improve the nutritional quality of sweet potatoes in order to address the health problems of people nutrient-deficient areas.Nanshu 012 is a new high-quality orange-fleshed sweet potato variety rich in carotenoid,bred by Nanchong Academy of Agricultural Sciences from the " Boga × Sanheshu" hybrid.In the regional test of sweet potato varieties in Sichuan Province during 2009-2010,the average fresh potato yield was 24600 kg/ha,the average dry matter percentage was 29.0 % and the average preserved sweet potato yield was 7152.0 kg/ha;in the production test,the average fresh potato yield was 28410.0 kg/ha and the average preserved sweet potato yield was 8734.5 kg/ha.The multi-point sampling analysis showed that the total sugar content of fresh potato was 5.28 %,protein content was 1.43 %,vitamin C content was 28.9 mg/100 g and carotenoid content was 5.21 mg/100 g.This variety was identified by Sichuan Crop Variety Approval Committee in March 2012,with high fresh potato yield,sweetness,rich carotenoid and resistance to black spot.It is an orange-fleshed sweet potato variety suitable for eating and food processing,and it should be planted in the plot with moderate fertility or above,and should be planted and harvested as early as possible.展开更多
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent of COVID-19,encodes several accessory proteins that have been shown to play crucial roles in regulating the innate immune response.Howeve...Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent of COVID-19,encodes several accessory proteins that have been shown to play crucial roles in regulating the innate immune response.However,their expressions in infected cells and immunogenicity in infected humans and mice are still not fully understood.This study utilized various techniques such as luciferase immunoprecipitation system(LIPS),immunofluorescence assay(IFA),and western blot(WB)to detect accessory protein-specific antibodies in sera of COVID-19 patients.Specific antibodies to proteins 3a,3b,7b,8 and 9c can be detected by LIPS,but only protein 3a antibody was detected by IFA or WB.Antibodies against proteins 3a and 7b were only detected in ICU patients,which may serve as a marker for predicting disease progression.Further,we investigated the expression of accessory proteins in SARS-CoV-2-infected cells and identified the expressions of proteins 3a,6,7a,8,and 9b.We also analyzed their ability to induce antibodies in immunized mice and found that only proteins 3a,6,7a,8,9b and 9c were able to induce measurable antibody productions,but these antibodies lacked neutralizing activities and did not protect mice from SARS-CoV-2 infection.Our findings validate the expression of SARS-CoV-2 accessory proteins and elucidate their humoral immune response,providing a basis for protein detection assays and their role in pathogenesis.展开更多
Massive scale of transactions with critical requirements become popular for emerging businesses,especially in E-commerce.One of the most representative applications is the promotional event running on Alibaba's pl...Massive scale of transactions with critical requirements become popular for emerging businesses,especially in E-commerce.One of the most representative applications is the promotional event running on Alibaba's platform on some special dates,widely expected by global customers.Although we have achieved significant progress in improving the scalability of transactional database systems(OLTP),the presence of contention operations in workloads is still one of the fundamental obstacles to performance improving.The reason is that the overhead of managing conflict transactions with concurrency control mechanisms is proportional to the amount of contentions.As a consequence,generating contented workloads is urgent to evaluate performance of modern OLTP database systems.Though we have kinds of standard benchmarks which provide some ways in simulating contentions,e.g.,skew distribution control of transactions,they can not control the generation of contention quantitatively;even worse,the simulation effectiveness of these methods is affected by the scale of data.So in this paper we design a scalable quantitative contention generation method with fine contention granularity control.We conduct a comprehensive set of experiments on popular opensourced DBMSs compared with the latest contention simulation method to demonstrate the effectiveness of our generation work.展开更多
Dear Editor,To date,seven human coronaviruses(HCoVs)have been identified,among which the highly pathogenic severe acute respiratory syndrome-associated coronavirus(SARS-CoV),Middle East respiratory syndrome coronaviru...Dear Editor,To date,seven human coronaviruses(HCoVs)have been identified,among which the highly pathogenic severe acute respiratory syndrome-associated coronavirus(SARS-CoV),Middle East respiratory syndrome coronavirus(MERS-CoV),and severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)have caused public health disasters worldwide.展开更多
Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the causative agent of the unprecedented coronavirus disease 2019(COVID-19).SARS-CoV-2 entry into host cells is mediated by the viral transmemb...Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the causative agent of the unprecedented coronavirus disease 2019(COVID-19).SARS-CoV-2 entry into host cells is mediated by the viral transmembrane spike(S)glycoprotein that forms homotrimers protruding from the viral surface.展开更多
Disease progression prediction and therapeutic drug target discovery for Coronavirus disease 2019(COVID-19)are particularly important,as there is still no effective strategy for severe COVID-19 patient treatment.Herei...Disease progression prediction and therapeutic drug target discovery for Coronavirus disease 2019(COVID-19)are particularly important,as there is still no effective strategy for severe COVID-19 patient treatment.Herein,we performed multi-platform omics analysis of serial plasma and urine samples collected from patients during the course of COVID-19.Integrative analyses of these omics data revealed several potential therapeutic targets,such as ANXA1 and CLEC3B.Molecular changes in plasma indicated dysregulation of macrophage and suppression of T cell functions in severe patients compared to those in non-severe patients.Further,we chose 25 important molecular signatures as potential biomarkers for the prediction of disease severity.The prediction power was validated using corresponding urine samples and plasma samples from new COVID-19 patient cohort,with AUC reached to 0.904 and 0.988,respectively.In conclusion,our omics data proposed not only potential therapeutic targets,but also biomarkers for understanding the pathogenesis of severe COVID-19.展开更多
Nitrogen doped carbon species(NC)was introduced into Fe based catalyst and applied into propane non-oxidative dehydrogenation.With characterizations of XRD,TEM and XPS,it was deduced that the NC modification induces t...Nitrogen doped carbon species(NC)was introduced into Fe based catalyst and applied into propane non-oxidative dehydrogenation.With characterizations of XRD,TEM and XPS,it was deduced that the NC modification induces the electrons transferred from NC species to Fe species,leading to electron-rich Fe species.Consequently,propane conversion and propene selectivity were increased by suppressing the by-products formation.It was also confirmed that such a modification strategy could be also extended to ones with different supports,iron precursors,and NC precursors.展开更多
Sintering inhibition of a catalyst at high temperatures is a challenge during heterogeneous catalysis. In this paper, we report that hexagonal boron nitride(h-BN) is an optimal material for anti-sintering γ-Al_(2)O_(...Sintering inhibition of a catalyst at high temperatures is a challenge during heterogeneous catalysis. In this paper, we report that hexagonal boron nitride(h-BN) is an optimal material for anti-sintering γ-Al_(2)O_(3)-supported Pt nanoparticles(NPs) originating from the high thermal conductivity of h-BN. The high thermal conductivity of h-BN ensures maximal heat dissipation from Pt NPs to γ-Al_(2)O_(3),thereby causing both Ostwald ripening and particle coalescence of Pt NPs to be decelerated at elevated temperatures.Inhibition of Pt NP sintering is also shown in the reducible TiO^(2-)supported Pt NPs with the help of h-BN. The proposed anti-sintering strategy using thermal management is universal, providing new insight into the design of anti-sintering materials and structures for a wide range of applications in heterogeneous catalysis.展开更多
Benchmarks play a crucial role in database performance evaluation,and have been effectively promoting the development of database management systems.With critical transaction processing requirements of new application...Benchmarks play a crucial role in database performance evaluation,and have been effectively promoting the development of database management systems.With critical transaction processing requirements of new applications,we see an explosion of innovative database technologies for dealing with highly intensive transaction workloads(OLTP)with the obvious characteristics of sharp dynamics,terrificskewness,high contention,or high concurrency(abbr.DSC^2),which can not be well described or evaluated by current standard benchmarks.In this paper,based on the representative SecKill applications,we define a pacakge of workloads simulating intensive transactional processing requirements.And we create a general and flexible benchmark framework PeakBench for evaluating intensive OLTP workloads on databases.We are the first work to have full control on simulating DSC^2,especially for the fine granularity control for contention generation.With a comprehensive set of experiments conducted on popular open sourced DBMSs compared with the other representative OLTP benchmarks,we completely demonstrate the usefulness of PeakBench.展开更多
基金supported by“Heart Health Research Fund,”Special Fund for Cardiovascular Disease Development of China,China International Medical Foundation(Grant No.Z-2019-42-1908-3),Tianjin Key Medical Discipline(Specialty)Construction Project,and the Young Talents Training Program of Tianjin First Central Hospital.
文摘Background:Preeclampsia(PE),characterized by hypertension and proteinuria,leads to serious maternal and infant complications.Uridine-cytidine kinase 2(UCK2)belongs to the UCK family,a class of enzymes that catalyzes the conversion of uridine and cytidine to monophosphate form.However,the role of UCK2 in PE has not been reported.Methods:The expression of UCK2 was detected in the placenta of PE patients and N(ω)-nitro-L-arginine methyl esterinduced PE mouse model.Through forced up-regulation or down-regulation of UCK2 in vitro,we examined the effects of UCK2 on the proliferation,apoptosis,migration,and invasion of trophoblast cells.Stattic,the inhibitor of STAT3 pathway,was used to investigate whether the STAT3 pathway mediates the biological function of UCK2 in trophoblast cells.Results:The present study found that UCK2 showed low expression in the placenta of PE patients and PE mouse model.MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)and flow cytometry assays verified that up-regulation of UCK2 promoted the proliferation of trophoblast cells,while the silence of UCK2 suppressed cell proliferation.Besides,flow cytometry and TdT-mediated dUTP Nick-End Labeling assays demonstrated that knockdown of UCK2 resulted in apoptosis of trophoblast cells.The wound healing and transwell assays showed that the migration and invasion activities of the trophoblast cells were facilitated by the overexpression of UCK2 and were blocked by the silence of UCK2.Furthermore,the expression of phosphorylated STAT3 was increased with the upregulation of UCK2 and decreased with the inhibition of UCK2.When the STAT3 pathway was blocked by its inhibitor stattic,the promotion effects of UCK2 on trophoblast cells were suppressed.Conclusion:UCK2 promotes the proliferation,migration,and invasion of trophoblast cells,and these effects may be partly mediated by the activation of the STAT3 pathway.
基金supported by the National Basic Research Program of China (2011CB201400 and 2011CB808700)the National Natural Science Foundation of China (21373019, 21173008 and 21433001)
文摘The aerobic oxidation of glycerol provides an economically viable route to glyceraldehyde, dihydroxyacetone and glyceric acid with versatile applications, for which monometallic Pt, Au and Pd and bimetallic Au-Pt, Au- Pd and Pt-Pd catalysts on TiO2 were examined under base-free conditions. Pt exhibited a superior activity relative to Pd, and Au-Pd and Pt-Pd while Au was essentially inactive. The presence of Au on the Au-Pt/TiO2 catalysts led to their higher activities （normalized per Pt atom） in a wide range of Au/Pt atomic ratios （i.e. 1/3-7/1 ）, and the one with the Au/Pt ratio of 3/1 exhibited the highest activity. Such promoting effect is ascribed to the increased electron density on Pt via the electron transfer from Au to Pt, as characterized by the temperature-programmed desorption of CO and infra-red spectroscopy for CO adsorption. Meanwhile, the presence of Au on Au-Pt/TiO2, most like due to the observed electron transfer, changed the product selectivity, and facilitated the oxidation of the secondary hydroxyl groups in glycerol, leading to the favorable formation of dihydroxyacetone over glyceraldehyde and glyceric acid that were derived from the oxidation of the primary hydroxyl groups. The synergetic effect between Au and Pt demonstrates the feasibility in the efficient oxidation of glycerol to the targeted products, for example, by rational tuning of the electronic properties of metal catalysts.
文摘In order to measure the residual stress components in an elastic thin plate, the hole-drilling strain-gage method has been used. This method enables to determine the relation between the magnitudes and directions of the principal stresses and the strain relaxation about the hole. In the existing analytical models based on stress field, the formulations associated with the hole-drilling method are based on the assumption of an infinite plate, this may cause some errors for a finite plate and it’s difficult to validate these solutions by FE methods. Furthermore, in the composite, the displacement field is continuous but the stress field is not necessarily continuous, the displacement field based method has to be used. In the present paper an analyt-ical model based on a displacement field described by a function with coefficients to determine for a finite round thin plate is presented. The coefficients used in the displacement field are independent on the three residual stress components, and they are determined by minimization of the internal strain energy during the hole-drilling process. Once the coefficients in the dis-placement field are determined, three strains measured in three radial directions are utilized to determine the three residual stress components. The proposed analytical model can be also adapted to infinite plate by assuming that the diameter of the round plate tends to infinite.
基金Supported by"125"Major Scientific and Technological Project of Education Department of Guizhou Province[QJHZDZXZ(2012)013]Guiyang Science and Technology Bureau Project[2012204]
文摘This study was conducted to investigate the gene expression in fructose-fed rat skeletal muscle by cDNA chip which could provide support to elucidate the molecular mechanisms underlying insulin resistance. The rats were divided into two groups, one of which was normal control and the other was fed with fructose-rich diet. The mRNA was isolated and purified from the skeletal muscle of two groups. The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and Cy5-dUTP separately to prepare hybridization probes. The mixed probes were hybridized to cDNA microarray. The microarray was scanned, analyzed and repeated for two times. Among the total 4 096 tested genes, 140 genes were differently expressed, 62 up-regulated,78 down-regulated, the expression of Ptprd and Gilz and multiple genes of oxidative metabolism is associated in insulin resistance. The differential expression of gene may be related to the pathogenesis of insulin resistance.
基金supported in part by the National Natural Science Foundation of China(81790624,81630010)Top-Notch Talent Program of Hubei Province and Tongji Hospital(2021YBJRC005)。
文摘Fulminant myocarditis is an acute diffuse inflammatory disease of myocardium.It is characterized by acute onset,rapid progress and high risk of death.Its pathogenesis involves excessive immune activation of the innate immune system and formation of inflammatory storm.According to China’s practical experience,the adoption of the“life support-based comprehensive treatment regimen”(with mechanical circulation support and immunomodulation therapy as the core)can significantly improve the survival rate and long-term prognosis.Special emphasis is placed on very early identification,very early diagnosis,very early prediction and very early treatment.
基金Project of Bureau for Disease Control and Prevention,National Health Commission(T2021-ZC02)Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-007).
文摘To improve the standard screening, diagnosis, and treatment of hypertension in patients in China;realize the standardization of clinical practice of hypertension;and improve the prevention and control level of hypertension in China, it is both important and necessary to develop a clinical practice guideline for hypertension according to a recognized methodology. Jointly sponsored by the National Center for Cardiovascular Diseases, Chinese Medical Doctor Association, Hypertension Committee of the Chinese Medical Doctor Association, Chinese Society of Cardiology, and Hypertension Committee of Cross-Straits Medicine Exchange Association, the “Chinese Clinical Practice Guidelines of Hypertension” was proposed. Research Unit of Evidence-Based Evaluation and Guidelines, Chinese Academy of Medical Sciences, Guideline and Standards Research Centre of Chinese Medical Association Publishing House, Lanzhou University Institute of Health Data Science, and Lanzhou University GRADE Center will provide methodological support for the guidelines.
基金Supported by Modern Agricultural Industry Technology System Construction Project(CARS-11-C-23)Nanchong Comprehensive Experiment Station Project(CARS-11-C-23)+1 种基金HarvestPlus Fund(HP8272-03)Key Research Project of Sichuan Province(2016NYZ0049)
文摘The carotenoid in sweet potato has a high health value for the human body,and Harvest Plus has also carried out the breeding to improve the nutritional quality of sweet potatoes in order to address the health problems of people nutrient-deficient areas.Nanshu 012 is a new high-quality orange-fleshed sweet potato variety rich in carotenoid,bred by Nanchong Academy of Agricultural Sciences from the " Boga × Sanheshu" hybrid.In the regional test of sweet potato varieties in Sichuan Province during 2009-2010,the average fresh potato yield was 24600 kg/ha,the average dry matter percentage was 29.0 % and the average preserved sweet potato yield was 7152.0 kg/ha;in the production test,the average fresh potato yield was 28410.0 kg/ha and the average preserved sweet potato yield was 8734.5 kg/ha.The multi-point sampling analysis showed that the total sugar content of fresh potato was 5.28 %,protein content was 1.43 %,vitamin C content was 28.9 mg/100 g and carotenoid content was 5.21 mg/100 g.This variety was identified by Sichuan Crop Variety Approval Committee in March 2012,with high fresh potato yield,sweetness,rich carotenoid and resistance to black spot.It is an orange-fleshed sweet potato variety suitable for eating and food processing,and it should be planted in the plot with moderate fertility or above,and should be planted and harvested as early as possible.
基金supported by grants from the National Natural Science Foundation of China(82002127,81971500,82025001,82172240)National Key R&D Program of China(2021YFC2301700,2022YFC2604100)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2022B1515020059,2021B1515130005)R&D Program of Guangzhou Laboratory(EKPG21-30-2).
文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent of COVID-19,encodes several accessory proteins that have been shown to play crucial roles in regulating the innate immune response.However,their expressions in infected cells and immunogenicity in infected humans and mice are still not fully understood.This study utilized various techniques such as luciferase immunoprecipitation system(LIPS),immunofluorescence assay(IFA),and western blot(WB)to detect accessory protein-specific antibodies in sera of COVID-19 patients.Specific antibodies to proteins 3a,3b,7b,8 and 9c can be detected by LIPS,but only protein 3a antibody was detected by IFA or WB.Antibodies against proteins 3a and 7b were only detected in ICU patients,which may serve as a marker for predicting disease progression.Further,we investigated the expression of accessory proteins in SARS-CoV-2-infected cells and identified the expressions of proteins 3a,6,7a,8,and 9b.We also analyzed their ability to induce antibodies in immunized mice and found that only proteins 3a,6,7a,8,9b and 9c were able to induce measurable antibody productions,but these antibodies lacked neutralizing activities and did not protect mice from SARS-CoV-2 infection.Our findings validate the expression of SARS-CoV-2 accessory proteins and elucidate their humoral immune response,providing a basis for protein detection assays and their role in pathogenesis.
基金supported by the National Natural Science Foundation of China(Grant No.62072179)ECNUOceanBase Joint Lab of Distributed Database System and 2020 the Key Software Adaptation and Verification Project(Database).
文摘Massive scale of transactions with critical requirements become popular for emerging businesses,especially in E-commerce.One of the most representative applications is the promotional event running on Alibaba's platform on some special dates,widely expected by global customers.Although we have achieved significant progress in improving the scalability of transactional database systems(OLTP),the presence of contention operations in workloads is still one of the fundamental obstacles to performance improving.The reason is that the overhead of managing conflict transactions with concurrency control mechanisms is proportional to the amount of contentions.As a consequence,generating contented workloads is urgent to evaluate performance of modern OLTP database systems.Though we have kinds of standard benchmarks which provide some ways in simulating contentions,e.g.,skew distribution control of transactions,they can not control the generation of contention quantitatively;even worse,the simulation effectiveness of these methods is affected by the scale of data.So in this paper we design a scalable quantitative contention generation method with fine contention granularity control.We conduct a comprehensive set of experiments on popular opensourced DBMSs compared with the latest contention simulation method to demonstrate the effectiveness of our generation work.
基金This study was funded by grants from the National Key Research and Development Program(2021YFC2300603)the National Science Foundation of China(32170157,Key program 81830017,and 81902051),Taishan Scholarship(No.tspd20181201)+1 种基金Major Basic Research Project of Shandong Natural Science Foundation(No.ZR2020ZD12)Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong,Key Research and Development Program of Shandong(2019GSF108238).We thank Professor Jincun Zhao(the First Affiliated Hospital of Guangzhou Medical University)for the friendly gifted HCoV-229E virus and S protein expression plasmids of HCoV-NL63 and HCoV-229E.We thank the Translational Medicine Core Facility of Shandong University for the consultation and instrument availability that supported this work.
文摘Dear Editor,To date,seven human coronaviruses(HCoVs)have been identified,among which the highly pathogenic severe acute respiratory syndrome-associated coronavirus(SARS-CoV),Middle East respiratory syndrome coronavirus(MERS-CoV),and severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)have caused public health disasters worldwide.
基金This study was funded by grants from Taishan Scholarship(No.tspd20181201)the National Science Foundation of China(Key program 81830017 and No.81902051,81902443)+3 种基金the National Natural Science Fund for Outstanding Youth Fund(81425012)Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong,Key Research and Development Program of Shandong(2019GSF108238)the COVID-19 emergency tackling research project of Shandong University(Grant No.2020XGB03)the Natural Science Foundation of Jiangsu Province(BK20200225)。
文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the causative agent of the unprecedented coronavirus disease 2019(COVID-19).SARS-CoV-2 entry into host cells is mediated by the viral transmembrane spike(S)glycoprotein that forms homotrimers protruding from the viral surface.
基金This work is supported by the grants from The National Key Research and Development Program of China(2018YFC1200100 to JC2L)National Science and Technology Major Project(2018ZX10301403 to JCZ.)+5 种基金the emergency grants for prevention and control of SARS-CoV-2 of Ministry of Science and Technology of Guangdong province(2020A111128008,2020B111112003,2018B020207013,2020B111108001 and 2020B1111320003 to JCZ,2020B1111330001 to NZ.)The National Program on Key Basic Research Project(2017YFC0906702 to Y.W.)National Key Technology R&D Program(2018YFC1311900 to N2.)Guangdong Science and Technology Foundation(2019B030316028,2020A1515010911 to NZ.)Guangzhou Medical University High-level University Innovation Team Training Program(Guangzhou Medical University released[2017]No.159 to JCZ and JX2.)111 project(D18010 to JCZI ).We thank the patients who took part in this study.
文摘Disease progression prediction and therapeutic drug target discovery for Coronavirus disease 2019(COVID-19)are particularly important,as there is still no effective strategy for severe COVID-19 patient treatment.Herein,we performed multi-platform omics analysis of serial plasma and urine samples collected from patients during the course of COVID-19.Integrative analyses of these omics data revealed several potential therapeutic targets,such as ANXA1 and CLEC3B.Molecular changes in plasma indicated dysregulation of macrophage and suppression of T cell functions in severe patients compared to those in non-severe patients.Further,we chose 25 important molecular signatures as potential biomarkers for the prediction of disease severity.The prediction power was validated using corresponding urine samples and plasma samples from new COVID-19 patient cohort,with AUC reached to 0.904 and 0.988,respectively.In conclusion,our omics data proposed not only potential therapeutic targets,but also biomarkers for understanding the pathogenesis of severe COVID-19.
基金the National key Research and Development Program Nanotechnology Specific Project(No.2020YFA0210900)the National Natural Science Foundation of China(Grant Nos.21961132026,21878331,21802167,91645108)Science Foundation of China University of Petroleum,Beijing(Nos.C201604).
文摘Nitrogen doped carbon species(NC)was introduced into Fe based catalyst and applied into propane non-oxidative dehydrogenation.With characterizations of XRD,TEM and XPS,it was deduced that the NC modification induces the electrons transferred from NC species to Fe species,leading to electron-rich Fe species.Consequently,propane conversion and propene selectivity were increased by suppressing the by-products formation.It was also confirmed that such a modification strategy could be also extended to ones with different supports,iron precursors,and NC precursors.
基金supported by the National Natural Science Foundation of China (21961132026,51888103,21878331,51606192,91645108 and U1162117)the Nanotechnology Specific Project of the National Key Research and Development Program (2020YFA0210900)+1 种基金the CAS Pioneer Hundred Talents Programthe Science Foundation of China University of Petroleum,Beijing (C201604)。
文摘Sintering inhibition of a catalyst at high temperatures is a challenge during heterogeneous catalysis. In this paper, we report that hexagonal boron nitride(h-BN) is an optimal material for anti-sintering γ-Al_(2)O_(3)-supported Pt nanoparticles(NPs) originating from the high thermal conductivity of h-BN. The high thermal conductivity of h-BN ensures maximal heat dissipation from Pt NPs to γ-Al_(2)O_(3),thereby causing both Ostwald ripening and particle coalescence of Pt NPs to be decelerated at elevated temperatures.Inhibition of Pt NP sintering is also shown in the reducible TiO^(2-)supported Pt NPs with the help of h-BN. The proposed anti-sintering strategy using thermal management is universal, providing new insight into the design of anti-sintering materials and structures for a wide range of applications in heterogeneous catalysis.
基金We are partially supported by the Key Program of National Natural Science Foundation of China(2018YFB1003402)the National Natural Science Foundation of China(Grant No.61432006).
文摘Benchmarks play a crucial role in database performance evaluation,and have been effectively promoting the development of database management systems.With critical transaction processing requirements of new applications,we see an explosion of innovative database technologies for dealing with highly intensive transaction workloads(OLTP)with the obvious characteristics of sharp dynamics,terrificskewness,high contention,or high concurrency(abbr.DSC^2),which can not be well described or evaluated by current standard benchmarks.In this paper,based on the representative SecKill applications,we define a pacakge of workloads simulating intensive transactional processing requirements.And we create a general and flexible benchmark framework PeakBench for evaluating intensive OLTP workloads on databases.We are the first work to have full control on simulating DSC^2,especially for the fine granularity control for contention generation.With a comprehensive set of experiments conducted on popular open sourced DBMSs compared with the other representative OLTP benchmarks,we completely demonstrate the usefulness of PeakBench.
