Objective To investigate the effects of propofol and sevoflurane on neurological recovery of traumatic brain injury(TBI)patients in the early postoperative stage.Methods We retrospectively analyzed the clinical data o...Objective To investigate the effects of propofol and sevoflurane on neurological recovery of traumatic brain injury(TBI)patients in the early postoperative stage.Methods We retrospectively analyzed the clinical data of TBI patients who underwent craniotomy or decompressive craniectomy.Generalized additive mixed model(GAMM)was used to analyze effects of propofol and sevoflurane on Glasgow Coma Scale(GCS)on postoperative days 1,3,and 7.Multivariate regression analysis was used to analyze effects of the two anesthetics on Glasgow Outcome Scale(GOS)at discharge.Results A total of 340 TBI patients were enrolled in this study.There were 110 TBI patients who underwent craniotomy including 75 in the propofol group and 35 in the sevoflurane group,and 134 patients who underwent decompressive craniectomy including 63 in the propofol group and 71 in the sevoflurane group.It showed no significant difference in GCS at admission between the propofol and the sevoflurane groups among craniotomy patients(β=0.75,95%CI:-0.55 to 2.05,P=0.260).However,elevation in GCS from baseline was 1.73 points(95%CI:-2.81 to-0.66,P=0.002)less in the sevoflurane group than that in the propofol group on postoperative day 1,2.03 points(95%CI:-3.14 to-0.91,P 0.001)less on day 3,and 1.31 points(95%CI:-2.43 to-0.19,P=0.022)less on day 7.The risk of unfavorable GOS(GOS 1,2,and 3)at discharge was higher in the sevoflurane group(OR=4.93,95%CI:1.05 to 23.03,P=0.043).No significant difference was observed among two-group decompressive craniectomy patients in GCS and GOS.Conclusions Compared to propofol,sevoflurane was associated with worse neurological recovery during the hospital stay in TBI patients undergoing craniotomy.This difference was not detected in TBI patients undergoing decompressive craniectomy.展开更多
Background:Homeobox B8(HOXB8),a member of HOX family,plays a key role in the development of colorectal cancer(CRC).However,the function of HOXB8 in oxaliplatin(OXA)resistance in CRC is still unclear.This study investi...Background:Homeobox B8(HOXB8),a member of HOX family,plays a key role in the development of colorectal cancer(CRC).However,the function of HOXB8 in oxaliplatin(OXA)resistance in CRC is still unclear.This study investigated the role and precise molecular mechanism of HOXB8 in OXA-resistant CRC cells.Methods:The cell viability was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide(MTT)assay,and the colony forming ability was determined by colony formation assay.The silencing RNA(siRNA)approach was used to knockdown HOXB8 in CRC cells while the lentiviral transfection system was used to establish stable HOXB8 overexpressing CRC cells.The protein and mRNA levels were evaluated by western blot and real-time reverse transcription-polymerase chain reaction.Results:HOXB8 expression was upregulated in OXA-resistant HCT116 cells(HCT116/OXA)compared to its level in the parent HCT116 cells.Knockdown of HOXB8 significantly inhibited CRC cell growth by suppressing the signal transducer and activator of transcription 3(STAT3)pathway.HOXB8 knockdown also potentiated cytotoxicity of OXA in CRC cells.Inversely,HOXB8 overexpression attenuated OXAinduced growth inhibition of HCT116 cells and RKO cells by activating STAT3 signaling.HOXB8 knockdown effectively inhibited HCT116/OXA cell viability regardless of OXA treatment by suppressing STAT3 signaling.Conclusions:These results shed light on the important functions of HOXB8 in OXA-resistant CRC and suggested that targeting HOXB8 might be an effective therapeutic strategy for select OXA-resistant CRC patients.展开更多
Hyperforin is an antidepressant drug that has unstable therapeutic effects, due to its poor ability to cross the blood-brain barrier. Borneol and electroacupuncture have both been found to increase the permeability of...Hyperforin is an antidepressant drug that has unstable therapeutic effects, due to its poor ability to cross the blood-brain barrier. Borneol and electroacupuncture have both been found to increase the permeability of the blood-brain barrier. As such, the current study examined the distribution of hyperforin in the rat brain, and the effects on the brain distribution of hyperforin of borneol alone （orally administered）, and borneol combined with electroacupuncture treatment. High-performance liquid chromatography technology and pharmacokinetic analysis revealed that treatment with borneol alone （300, 600 mg/kg） increased peak concentration and the area under the curve for hyperforin in the brain. In addition, the bioavailability of hyperforin in rat brain increased by 42.7%. However, increasing the dose of borneol dose did not appear to increase the distribution of hyperforin in the brain. Importantly, applying electroacupuncture at Baihui （GV 20） or Yamen （GV 15） appeared to enhance the brain-delivery effects of borneol, although this effect was weak. Overall, our results indicated that borneol alone or combined with electroacupuncture can provide promising strategies for brain-targeted delivery in central nervous system therapy.展开更多
Type 2 diabetes mellitus (T2DM) has become a prevalent health problem in China,especially in urban areas.Early prevention strategies are needed to reduce the associated mortality and morbidity.We applied the combinati...Type 2 diabetes mellitus (T2DM) has become a prevalent health problem in China,especially in urban areas.Early prevention strategies are needed to reduce the associated mortality and morbidity.We applied the combination of rules and different machine learning techniques to assess the risk of development of T2DM in an urban Chinese adult population.A retrospective analysis was performed on 8000 people with non-diabetes and 3845 people with T2DM in Nanjing.Multilayer Perceptron (MLP),AdaBoost (AD),Trees Random Forest (TRF),Support Vector Machine (SVM),and Gradient Tree Boosting (GTB) machine learning techniques with 10 cross validation methods were used with the proposed model for the prediction of the risk of development of T2DM.The performance of these models was evaluated with accuracy,precision,sensitivity,specificity,and area under receiver operating characteristic (ROC) curve (AUC).After comparison,the prediction accuracy of the different five machine models was 0.87,0.86,0.86,0.86 and 0.86 respectively.The combination model using the same voting weight of each component was built on T2DM,which was performed better than individual models.The findings indicate that,combining machine learning models could provide an accurate assessment model for T2DM risk prediction.展开更多
BACKGROUND Carotid artery cross-clamping during carotid endarterectomy(CEA)may damage local cerebral perfusion and induce cerebral ischemia–reperfusion injury to activate local inflammatory responses.Neutrophil-to-ly...BACKGROUND Carotid artery cross-clamping during carotid endarterectomy(CEA)may damage local cerebral perfusion and induce cerebral ischemia–reperfusion injury to activate local inflammatory responses.Neutrophil-to-lymphocyte ratio(NLR)is an indicator that reflects systemic inflammation.However,the correlation between NLR and complications after CEA remains unclear.AIM To investigate the association between NLR and major complications after surgery in patients undergoing CEA.METHODS This retrospective cohort study included patients who received CEA between January 2016 and July 2018 at Beijing Tiantan Hospital.Neutrophil and lymphocyte counts in whole blood within 24 h after CEA were collected.The primary outcome was the composite of major postoperative complications including neurological,pulmonary,cardiovascular and acute kidney injuries.The secondary outcomes included infections,fever,deep venous thrombosis,length of hospitalization and cost of hospitalization.Statistical analyses were performed using EmpowerStats software and R software.RESULTS A total of 224 patients who received CEA were screened for review and 206 were included in the statistical analyses;of whom,40(19.42%)developed major postoperative complications.NLR within 24 h after CEA was significantly correlated with major postoperative complications(P=0.026).After confounding factors were adjusted,the odds ratio was 1.15(95%CI:1.03–1.29,P=0.014).The incidence of major postoperative complications in the high NLR group was 8.47 times that in the low NLR group(P=0.002).CONCLUSION NLR is associated with major postoperative complications in patients undergoing CEA.展开更多
The pressure and horizontal particle velocity combined descriptions in the very low frequency acoustic field of shallow wa- ter integrated with the concept of effective depth of Pekeris wave- guide is proposed, especi...The pressure and horizontal particle velocity combined descriptions in the very low frequency acoustic field of shallow wa- ter integrated with the concept of effective depth of Pekeris wave- guide is proposed, especially the active component of the pressure and horizontal particle velocity cross-spectrum, also called ho- rizontal complex cross acoustic intensity, when only two normal modes are trapped in the waveguide. Both the approximate theo- retic analysis and the numerical results show that the sign of the horizontal complex cross acoustic intensity active component is independent of the range when vertically deployed receiving dual sensors are placed in appropriate depths, the sum of which is equal to the waveguide effective depth, so it can be used to tell whether the sound source is near the surface or underwater; while the range rate is expected to be measured by utilizing the sign distribution characteristic of the reactive component. The further robustness analysis of the depth classification algorithm shows that the existence of shear waves in semi infinite basement and the change of acoustic velocity profiles have few effects on the application of this method, and the seabed attenuation will limit the detection range, but the algorithm still has a good robustness in the valid detection range.展开更多
The extraction fraction E and overall volumetric mass transfer coefficient kka of TBP extracting butyric acid pro- cess in confined impinging jet reactors （CIJR） with two jets were investigated. The main variables t...The extraction fraction E and overall volumetric mass transfer coefficient kka of TBP extracting butyric acid pro- cess in confined impinging jet reactors （CIJR） with two jets were investigated. The main variables tested were the concentration of tri-butyl-phosphate （TBP） and butyric acid, the impinging velocity V, the impinging velocityratio of two phases Vorg/Vaq, the nozzle inner diameter di and the distance L between the jet axes and the top wall of the impinging chamber. The results showed that E and kLa increase with an increase of the impinging velocity V, the concentration ofTBP Corg, and the impinging velocity ratio Vor/Vaq. However, E and kta decrease with an increase of the inner diameter d1 from 1 to 2 mm, the concentration of butyric acid Caq from 0.5% （v/v） to 2% （v/v）. The factor L ranging from 3 to 11 mm has a negligible effect on E and kLa. A correlation on these variables and kLa was proposed based on the experimental data. These results indicated good mass transfer oerformance of CIJR in the extraction operation.展开更多
基金Beijing Natural Sciences Foundation(7173255)Beijing Municipal Administration of Hospital Incubating Program(PX2019019).
文摘Objective To investigate the effects of propofol and sevoflurane on neurological recovery of traumatic brain injury(TBI)patients in the early postoperative stage.Methods We retrospectively analyzed the clinical data of TBI patients who underwent craniotomy or decompressive craniectomy.Generalized additive mixed model(GAMM)was used to analyze effects of propofol and sevoflurane on Glasgow Coma Scale(GCS)on postoperative days 1,3,and 7.Multivariate regression analysis was used to analyze effects of the two anesthetics on Glasgow Outcome Scale(GOS)at discharge.Results A total of 340 TBI patients were enrolled in this study.There were 110 TBI patients who underwent craniotomy including 75 in the propofol group and 35 in the sevoflurane group,and 134 patients who underwent decompressive craniectomy including 63 in the propofol group and 71 in the sevoflurane group.It showed no significant difference in GCS at admission between the propofol and the sevoflurane groups among craniotomy patients(β=0.75,95%CI:-0.55 to 2.05,P=0.260).However,elevation in GCS from baseline was 1.73 points(95%CI:-2.81 to-0.66,P=0.002)less in the sevoflurane group than that in the propofol group on postoperative day 1,2.03 points(95%CI:-3.14 to-0.91,P 0.001)less on day 3,and 1.31 points(95%CI:-2.43 to-0.19,P=0.022)less on day 7.The risk of unfavorable GOS(GOS 1,2,and 3)at discharge was higher in the sevoflurane group(OR=4.93,95%CI:1.05 to 23.03,P=0.043).No significant difference was observed among two-group decompressive craniectomy patients in GCS and GOS.Conclusions Compared to propofol,sevoflurane was associated with worse neurological recovery during the hospital stay in TBI patients undergoing craniotomy.This difference was not detected in TBI patients undergoing decompressive craniectomy.
基金supported by the Natural Science Foundation of Zhejiang Province(LZ22H160006)Huadong Medicine Joint Funds of the Zhejiang Provincial Natural Science Foundation of China(LHDMY22H160002)Wenzhou Municipal Science and Technology Bureau(Y20180085).
文摘Background:Homeobox B8(HOXB8),a member of HOX family,plays a key role in the development of colorectal cancer(CRC).However,the function of HOXB8 in oxaliplatin(OXA)resistance in CRC is still unclear.This study investigated the role and precise molecular mechanism of HOXB8 in OXA-resistant CRC cells.Methods:The cell viability was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide(MTT)assay,and the colony forming ability was determined by colony formation assay.The silencing RNA(siRNA)approach was used to knockdown HOXB8 in CRC cells while the lentiviral transfection system was used to establish stable HOXB8 overexpressing CRC cells.The protein and mRNA levels were evaluated by western blot and real-time reverse transcription-polymerase chain reaction.Results:HOXB8 expression was upregulated in OXA-resistant HCT116 cells(HCT116/OXA)compared to its level in the parent HCT116 cells.Knockdown of HOXB8 significantly inhibited CRC cell growth by suppressing the signal transducer and activator of transcription 3(STAT3)pathway.HOXB8 knockdown also potentiated cytotoxicity of OXA in CRC cells.Inversely,HOXB8 overexpression attenuated OXAinduced growth inhibition of HCT116 cells and RKO cells by activating STAT3 signaling.HOXB8 knockdown effectively inhibited HCT116/OXA cell viability regardless of OXA treatment by suppressing STAT3 signaling.Conclusions:These results shed light on the important functions of HOXB8 in OXA-resistant CRC and suggested that targeting HOXB8 might be an effective therapeutic strategy for select OXA-resistant CRC patients.
基金the National Natural Science Foundation of China,No: 81001651Opening Project from Key Laboratory of Integrative Acupuncture and Drugs Constructed by Jiangsu Provincial and Chinese Education Ministry,No: KJA200905
文摘Hyperforin is an antidepressant drug that has unstable therapeutic effects, due to its poor ability to cross the blood-brain barrier. Borneol and electroacupuncture have both been found to increase the permeability of the blood-brain barrier. As such, the current study examined the distribution of hyperforin in the rat brain, and the effects on the brain distribution of hyperforin of borneol alone （orally administered）, and borneol combined with electroacupuncture treatment. High-performance liquid chromatography technology and pharmacokinetic analysis revealed that treatment with borneol alone （300, 600 mg/kg） increased peak concentration and the area under the curve for hyperforin in the brain. In addition, the bioavailability of hyperforin in rat brain increased by 42.7%. However, increasing the dose of borneol dose did not appear to increase the distribution of hyperforin in the brain. Importantly, applying electroacupuncture at Baihui （GV 20） or Yamen （GV 15） appeared to enhance the brain-delivery effects of borneol, although this effect was weak. Overall, our results indicated that borneol alone or combined with electroacupuncture can provide promising strategies for brain-targeted delivery in central nervous system therapy.
基金This work was supported by grants from the National Natural Science Foundation of China (No.81570737, No.81370947, No.81570736, No.81770819, No.81500612, No.81400832, No.81600637, No.81600632, and No.81703294)the National Key Research and Development Program of China (No.2016YFC1304804 and No.2017YFC1309605)+4 种基金the Jiangsu Provincial Key Medical Discipline (No.ZDXKB2016012)the Key Project of Nanjing Clinical Medical Sciencethe Key Research and Development Program of Jiangsu Province of China (No.BE2015604 and No.BE2016606)the Jiangsu Provincial Medical Talent (No.ZDRCA2016062)the Nanjing Science and Technology Development Project (No.201605019).
文摘Type 2 diabetes mellitus (T2DM) has become a prevalent health problem in China,especially in urban areas.Early prevention strategies are needed to reduce the associated mortality and morbidity.We applied the combination of rules and different machine learning techniques to assess the risk of development of T2DM in an urban Chinese adult population.A retrospective analysis was performed on 8000 people with non-diabetes and 3845 people with T2DM in Nanjing.Multilayer Perceptron (MLP),AdaBoost (AD),Trees Random Forest (TRF),Support Vector Machine (SVM),and Gradient Tree Boosting (GTB) machine learning techniques with 10 cross validation methods were used with the proposed model for the prediction of the risk of development of T2DM.The performance of these models was evaluated with accuracy,precision,sensitivity,specificity,and area under receiver operating characteristic (ROC) curve (AUC).After comparison,the prediction accuracy of the different five machine models was 0.87,0.86,0.86,0.86 and 0.86 respectively.The combination model using the same voting weight of each component was built on T2DM,which was performed better than individual models.The findings indicate that,combining machine learning models could provide an accurate assessment model for T2DM risk prediction.
基金Clinical Medicine Development of Special Funding,No.ZYLX201708 and No.DFL20180502Beijing Municipal Administration of Hospitals Incubating Program,No.PX2017037+1 种基金Beijing Hospitals Authority Youth Programme,No.QML20190508Beijing Municipal Science&Technology Commission,No.Z191100006619067.
文摘BACKGROUND Carotid artery cross-clamping during carotid endarterectomy(CEA)may damage local cerebral perfusion and induce cerebral ischemia–reperfusion injury to activate local inflammatory responses.Neutrophil-to-lymphocyte ratio(NLR)is an indicator that reflects systemic inflammation.However,the correlation between NLR and complications after CEA remains unclear.AIM To investigate the association between NLR and major complications after surgery in patients undergoing CEA.METHODS This retrospective cohort study included patients who received CEA between January 2016 and July 2018 at Beijing Tiantan Hospital.Neutrophil and lymphocyte counts in whole blood within 24 h after CEA were collected.The primary outcome was the composite of major postoperative complications including neurological,pulmonary,cardiovascular and acute kidney injuries.The secondary outcomes included infections,fever,deep venous thrombosis,length of hospitalization and cost of hospitalization.Statistical analyses were performed using EmpowerStats software and R software.RESULTS A total of 224 patients who received CEA were screened for review and 206 were included in the statistical analyses;of whom,40(19.42%)developed major postoperative complications.NLR within 24 h after CEA was significantly correlated with major postoperative complications(P=0.026).After confounding factors were adjusted,the odds ratio was 1.15(95%CI:1.03–1.29,P=0.014).The incidence of major postoperative complications in the high NLR group was 8.47 times that in the low NLR group(P=0.002).CONCLUSION NLR is associated with major postoperative complications in patients undergoing CEA.
基金supported by the National Natural Science Foundation of China(1140440611374072)
文摘The pressure and horizontal particle velocity combined descriptions in the very low frequency acoustic field of shallow wa- ter integrated with the concept of effective depth of Pekeris wave- guide is proposed, especially the active component of the pressure and horizontal particle velocity cross-spectrum, also called ho- rizontal complex cross acoustic intensity, when only two normal modes are trapped in the waveguide. Both the approximate theo- retic analysis and the numerical results show that the sign of the horizontal complex cross acoustic intensity active component is independent of the range when vertically deployed receiving dual sensors are placed in appropriate depths, the sum of which is equal to the waveguide effective depth, so it can be used to tell whether the sound source is near the surface or underwater; while the range rate is expected to be measured by utilizing the sign distribution characteristic of the reactive component. The further robustness analysis of the depth classification algorithm shows that the existence of shear waves in semi infinite basement and the change of acoustic velocity profiles have few effects on the application of this method, and the seabed attenuation will limit the detection range, but the algorithm still has a good robustness in the valid detection range.
基金Supported by the National Natural Science Foundation of China(21206002,21376016)the State Key Laboratory of Chemical Engineering(SKL-Ch E-13A03)
文摘The extraction fraction E and overall volumetric mass transfer coefficient kka of TBP extracting butyric acid pro- cess in confined impinging jet reactors （CIJR） with two jets were investigated. The main variables tested were the concentration of tri-butyl-phosphate （TBP） and butyric acid, the impinging velocity V, the impinging velocityratio of two phases Vorg/Vaq, the nozzle inner diameter di and the distance L between the jet axes and the top wall of the impinging chamber. The results showed that E and kLa increase with an increase of the impinging velocity V, the concentration ofTBP Corg, and the impinging velocity ratio Vor/Vaq. However, E and kta decrease with an increase of the inner diameter d1 from 1 to 2 mm, the concentration of butyric acid Caq from 0.5% （v/v） to 2% （v/v）. The factor L ranging from 3 to 11 mm has a negligible effect on E and kLa. A correlation on these variables and kLa was proposed based on the experimental data. These results indicated good mass transfer oerformance of CIJR in the extraction operation.
