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Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus 被引量:5
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作者 Ying-xian Ma Chayanis Sutcharitchan +4 位作者 Xiao-die Li Qian Meng Xin Wang Shen Ji ya-jun cui 《Chinese Herbal Medicines》 CAS 2020年第4期367-374,共8页
Objective:As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images,here we developed a systemic procedure to be used in microscopic ident... Objective:As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images,here we developed a systemic procedure to be used in microscopic identification of Chinese medicines,which would lead to more objective,effective and accurate identification process.Methods:Spatholobi Caulis(Jixueteng in Chinese)was used as the specimen in the development of such procedure.Jixueteng samples were microscopically examined in bright-and dark-field microscopy.Microscopic images were obtained by regular,EDF,and image stitching techniques.Results:The microscopic images of the characteristics in pulverized Jixueteng were captured,thanks to EDF imaging and image stitching techniques which allowed the detailed and full sighting of each characteristic to be obtained simultaneously.Different layers in anatomical transverse section,including cork,phelloderm,cortex,phloem,cambium,xylem and pith,were distinctively observed.Moreover,by comparing images of bright-and dark-field microscopy,birefringent and non-birefringent components could readily be distinguished.Conclusion:With application of the developed procedure,high-definition,panoramic and microscopic images were acquired,which could be used as the reference images for microscopic identification of Chinese medicines. 展开更多
关键词 extended depth of field imaging image stitching microscopic identification polarized light PHARMACOGNOSY spatholobus suberectus Dunn
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Pharmacognostical study of Cynanchum stauntonii and Cynanchum glaucescens,botanical sources of TCM Baiqian 被引量:1
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作者 Chayanis Sutcharitchan Li-na Wang +3 位作者 Hang Zhang Xiao-die Li ya-jun cui Shen Ji 《Chinese Herbal Medicines》 CAS 2018年第4期379-387,共9页
Objective: Cynanchum stauntonii and Cynanchum glaucescens are botanical species of Baiqian(Cynanchi Stauntonii Rhizoma et Radix) in Chinese Pharmacopoeia, in which, however, there are no microscopic identification.... Objective: Cynanchum stauntonii and Cynanchum glaucescens are botanical species of Baiqian(Cynanchi Stauntonii Rhizoma et Radix) in Chinese Pharmacopoeia, in which, however, there are no microscopic identification. Therefore, we provided the morphological and microscopic identification of the crude drug for updating Chinese Pharmacopoeia.Methods: Twelve batches of C. stauntonii and three batches of C. glaucescens and their crude drugs were taxonomically, morphologically, and microscopically examined.Results: Taxonomically, C. stauntonii had narrowly lanceolate leaves with acuminate apex and 5 mm long petiole; Whereas C. glaucescens was oblong-lanceolate or oblong with rounded or acute apex in leaves,and had very short or no petiole. Morphologically, rhizomes of C. stauntonii and C. glaucescens both had hollow pith, but the hollow pith occupied about a half of the rhizome's diameter in C. stauntonii, whereas only a very small proportion of the overall diameter in C. glaucescens. Moreover, microscopic observation showed the difference in the proportion of xylem and in rhizome transverse-sections of the two species along with the difference in the size of the pith. Finally, laticifers and rhizome epidermal secretory cells were present in the powders of C. stauntonii, but absent from C. glaucescens.Conclusion: Based on observation of morphological and microscopic characteristics, the two species can be distinguished by the size of the pith, proportion of xylem of rhizomes, and crude drug powder characters such as laticifers and secretory cells. 展开更多
关键词 Baiqian Cynanchum stauntonii (Decne.) Schltr. ex Levl. Cynanchurn glaucescens (Decne.) Hand.-Mazz. microscopic Identification morphological Identification PHARMACOGNOSY
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