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Microdetermination of Residual Protein in Penicillin by Resonance Light Scattering Technique with m-Nitrophenylfluorone-Mo(Ⅳ) Complex
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作者 QinWEI DanWU +3 位作者 yahli JunHongWANG YanLIU BinBU 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第6期815-818,共4页
关键词 Protein MICROEMULSION m-NPF-Mo(VI) complex PENICILLIN resonance light scattering.
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The M Protein of SARS-CoV: Basic Structural and Immunological Properties 被引量:1
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作者 JunWang SiqiLiu +15 位作者 ChangqingZeng JianWang HuanmingYang YongwuHu JieWen LinTang HaijunZhang XiaoweiZhang yahli JingWang YujunHan GuoqingLi JianpingShi XiangjunTian FengJiang XiaoqianZhao 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2003年第2期118-130,共13页
We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predi... We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior. The M protein harbors a higher substitution rate (0.6% correlated to its size) among viral open reading frames (ORFs) from published data. The four substitutions detected in the M protein, which cause non-synonymous changes, can be classified into three types. One of them results in changes of pI (isoelectric point) and charge, affecting antigenicity. The second changes hydrophobicity of the TM region, and the third one relates to hydrophilicity of the interior structure. Phylogenetic tree building based on the variations of the M protein appears to support the non-human origin of SARS-CoV. To investigate its immunogenicity, we synthesized eight oligopeptides covering 69.2% of the entire ORF and screened them by using ELISA (enzyme-linked immunosorbent assay) with sera from SARS patients. The results confirmed our predictions on antigenic sites. 展开更多
关键词 SARS-COV the M protein enzyme immunoassay ANTIGENICITY
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The R Protein of SARS-CoV: Analyses of Structure and Function Based on Four Complete Genome Sequences of Isolates BJ01-BJ04
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作者 HaiyanSun XiaoweiZhang +17 位作者 JunZhow SonggangLi JunWang JianWang ShenghBi HuanmingYang ZuyuanXu HaiqingZhang XiangjunTian JiaJi WeiLi yahli WeiTian LiftWang ZizhangZhang JingXu WeiWei JinguiZhu 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2003年第2期155-165,共11页
The R (replicase) protein is the uniquely defined non-structural protein (NSP) responsible for RNA replication, mutation rate or fidelity, regulation of transcription in coronaviruses and many other ssRNA viruses. Bas... The R (replicase) protein is the uniquely defined non-structural protein (NSP) responsible for RNA replication, mutation rate or fidelity, regulation of transcription in coronaviruses and many other ssRNA viruses. Based on our complete genome sequences of four isolates (BJ01-BJ04) of SARS-CoV from Beijing, China, we analyzed the structure and predicted functions of the R protein in comparison with 13 other isolates of SARS-CoV and 6 other coronaviruses. The entire ORF (open-reading frame) encodes for two major enzyme activities, RNA-dependent RNA polymerase (RdRp) and proteinase activities. The R polyprotein undergoes a complex proteolytic process to produce 15 function-related peptides. A hydrophobic domain (HOD) and a hydrophilic domain (HID) are newly identified within NSP1. The substitution rate of the R protein is close to the average of the SARS-CoV genome. The functional domains in all NSPs of the R protein give different phylogenetic results that suggest their different mutation rate under selective pressure. Eleven highly conserved regions in RdRp and twelve cleavage sites by 3CLP (chymotrypsin-like protein) have been identified as potential drug targets. Findings suggest that it is possible to obtain information about the phy-logeny of SARS-CoV, as well as potential tools for drug design, genotyping and diagnostics of SARS. 展开更多
关键词 SARS SARS-COV RNA-dependent RNA polymerase RNA viruses PROTEOLYSIS
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