Pre-harvest water deficit(PHWD)plays an important role in sugar accumulation of citrus fruit.However,the mechanism is not known well.Here,it was confirmed that PHWD promoted sucrose accumulation of citrus fruit,but ha...Pre-harvest water deficit(PHWD)plays an important role in sugar accumulation of citrus fruit.However,the mechanism is not known well.Here,it was confirmed that PHWD promoted sucrose accumulation of citrus fruit,but had limited effect on fructose,glucose and total acid.A sucrose transporter,Cs SUT1,which localizes to the plasma membrane,was demonstrated to function in sucrose transport induced by PHWD.Compared to wild-type,Cs SUT1 overexpression in citrus calli stimulated sucrose,fructose and glucose accumulation,while its silencing in juice sacs reduced sucrose accumulation.Increased sugar accumulation in transgenic lines enhanced plant drought tolerance,and resulted in decreased electrolyte leakage,malondialdehyde and hydrogen peroxide contents,as well as increased superoxide dismutase activity and proline contents.An abscisic acid(ABA)-responsive transcription factor,Cs ABF3,was found with a same expression pattern with Cs SUT1 under PHWD.Yeast one-hybrid,electrophoretic mobility shift assay and dual-luciferase assays all revealed that Cs ABF3 directly bound with the Cs SUT1 promoter by ABA responsive elements.When Cs ABF3 was overexpressed in citrus calli,the sucrose,fructose and glucose concentration increased correspondingly.Further,transgenic studies demonstrated that Cs ABF3 could affect sucrose accumulation by regulating Cs SUT1.Overall,this study revealed a regulation of Cs ABF3 promoting Cs SUT1 expression and sucrose accumulation in response to PHWD.Our results provide a detail insight into the quality formation of citrus fruit.展开更多
An authoritative parenting style has been shown to promote children’s emotion regulation in European-American family studies.However,little is known about how sleep problems and the child’s sibling status in Chinese...An authoritative parenting style has been shown to promote children’s emotion regulation in European-American family studies.However,little is known about how sleep problems and the child’s sibling status in Chinese families affect this relationship.Based on family system theory,this study attempts to better understand the relationship between authoritative parenting style and emotion regulation.Mothers of preschool children in Chinese kindergartens completed questionnaires about their children’s sleep habits,their authoritative parenting styles,and children’s emotion regulation.A total of 531 children participated in this study.Results showed that authoritative parenting was positively associated with emotional regulation.Sleep problems mediated the effects of authoritative parenting style on emotion regulation.The child’s sibling status moderated the mediating effects of sleep problems in authoritative parenting and emotion regulation relationships.Specifically,the relationship between the authoritative parenting style and sleep problems was significant for only children,while birth order had no significant influence on the authoritative parenting style and sleep problems in two-child families.These findings suggest that a lowauthoritative parenting style predicts low emotion regulation through sleep problems,and this depends on the child’s sibling status,indicating that children without siblings may impair emotion regulation due to increased sleep problems.展开更多
基于1951—2010年英国东英吉利大学(East Anglia)气候研究中心降水资料来检验第六次国际耦合模式比较计划(Commentary on the Coupled Model Intercomparison Project Phase 6,CMIP6)模式资料中22套模式降水资料对中亚地区年平均降水的...基于1951—2010年英国东英吉利大学(East Anglia)气候研究中心降水资料来检验第六次国际耦合模式比较计划(Commentary on the Coupled Model Intercomparison Project Phase 6,CMIP6)模式资料中22套模式降水资料对中亚地区年平均降水的模拟能力,并挑选最优的一批模式资料来预估中亚未来降水的时空分布特征。结果表明:在2021—2060年不同排放情景下,多模式集合平均与历史值之差的降水气候态分布与1951—2010年间降水气候态分布特征明显不同,而均方差的分布特征基本相似,在春季和冬季,中亚未来降水趋势均体现出北部降水增长,南部降水减少的分布特征。未来中亚春季和冬季平均降水量在大部分地区始终出现变多趋势,不同排放情景下预估降水长期趋势变化的结果表明,中亚春季和冬季整体降水增长或减少趋势的强度均大于以往降水变化的强度。展开更多
In 2023, Latin America was confronted with increased domestic and international challenges, resulting in a stronger public desire for change. New social ideologies are continuing to emerge, and transformation through ...In 2023, Latin America was confronted with increased domestic and international challenges, resulting in a stronger public desire for change. New social ideologies are continuing to emerge, and transformation through reform has become a broad consensus. However, during the complicated reshaping of Latin America's political ecology, the swing of the political pendulum and the change of regimes are accelerating.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32172520)the earmarked fund for China Agriculture Research System(Grant No.CARS-26)。
文摘Pre-harvest water deficit(PHWD)plays an important role in sugar accumulation of citrus fruit.However,the mechanism is not known well.Here,it was confirmed that PHWD promoted sucrose accumulation of citrus fruit,but had limited effect on fructose,glucose and total acid.A sucrose transporter,Cs SUT1,which localizes to the plasma membrane,was demonstrated to function in sucrose transport induced by PHWD.Compared to wild-type,Cs SUT1 overexpression in citrus calli stimulated sucrose,fructose and glucose accumulation,while its silencing in juice sacs reduced sucrose accumulation.Increased sugar accumulation in transgenic lines enhanced plant drought tolerance,and resulted in decreased electrolyte leakage,malondialdehyde and hydrogen peroxide contents,as well as increased superoxide dismutase activity and proline contents.An abscisic acid(ABA)-responsive transcription factor,Cs ABF3,was found with a same expression pattern with Cs SUT1 under PHWD.Yeast one-hybrid,electrophoretic mobility shift assay and dual-luciferase assays all revealed that Cs ABF3 directly bound with the Cs SUT1 promoter by ABA responsive elements.When Cs ABF3 was overexpressed in citrus calli,the sucrose,fructose and glucose concentration increased correspondingly.Further,transgenic studies demonstrated that Cs ABF3 could affect sucrose accumulation by regulating Cs SUT1.Overall,this study revealed a regulation of Cs ABF3 promoting Cs SUT1 expression and sucrose accumulation in response to PHWD.Our results provide a detail insight into the quality formation of citrus fruit.
基金supported by the Guangdong Province Philosophy and Social Science Project(Grant No.GD22CJY12)the Young Innovation Talent Project of Guangdong Province(Grant No.2022WTSCX112)the Key Construction Discipline of Guangdong Province(Grant No.2022ZDJS061)to Yan Jin.
文摘An authoritative parenting style has been shown to promote children’s emotion regulation in European-American family studies.However,little is known about how sleep problems and the child’s sibling status in Chinese families affect this relationship.Based on family system theory,this study attempts to better understand the relationship between authoritative parenting style and emotion regulation.Mothers of preschool children in Chinese kindergartens completed questionnaires about their children’s sleep habits,their authoritative parenting styles,and children’s emotion regulation.A total of 531 children participated in this study.Results showed that authoritative parenting was positively associated with emotional regulation.Sleep problems mediated the effects of authoritative parenting style on emotion regulation.The child’s sibling status moderated the mediating effects of sleep problems in authoritative parenting and emotion regulation relationships.Specifically,the relationship between the authoritative parenting style and sleep problems was significant for only children,while birth order had no significant influence on the authoritative parenting style and sleep problems in two-child families.These findings suggest that a lowauthoritative parenting style predicts low emotion regulation through sleep problems,and this depends on the child’s sibling status,indicating that children without siblings may impair emotion regulation due to increased sleep problems.
文摘基于1951—2010年英国东英吉利大学(East Anglia)气候研究中心降水资料来检验第六次国际耦合模式比较计划(Commentary on the Coupled Model Intercomparison Project Phase 6,CMIP6)模式资料中22套模式降水资料对中亚地区年平均降水的模拟能力,并挑选最优的一批模式资料来预估中亚未来降水的时空分布特征。结果表明:在2021—2060年不同排放情景下,多模式集合平均与历史值之差的降水气候态分布与1951—2010年间降水气候态分布特征明显不同,而均方差的分布特征基本相似,在春季和冬季,中亚未来降水趋势均体现出北部降水增长,南部降水减少的分布特征。未来中亚春季和冬季平均降水量在大部分地区始终出现变多趋势,不同排放情景下预估降水长期趋势变化的结果表明,中亚春季和冬季整体降水增长或减少趋势的强度均大于以往降水变化的强度。
文摘In 2023, Latin America was confronted with increased domestic and international challenges, resulting in a stronger public desire for change. New social ideologies are continuing to emerge, and transformation through reform has become a broad consensus. However, during the complicated reshaping of Latin America's political ecology, the swing of the political pendulum and the change of regimes are accelerating.
