Endotoxin detection is an important step in drug characterization. Herein we found that a chemotherapeutic drug nanoformulation composed of irinotecan hydrochloride(CPT-11) and an amphiphilic molecule DSPE-mPEG_(2000)...Endotoxin detection is an important step in drug characterization. Herein we found that a chemotherapeutic drug nanoformulation composed of irinotecan hydrochloride(CPT-11) and an amphiphilic molecule DSPE-mPEG_(2000) can interfere with the limulus amebocyte lysate assay(LAL). Furthermore, the rabbit pyrogen test(RPT) results indicated that at a relatively high dosage, the drug irinotecan hydrochloride can induce a hypothermia effect which may render the RPT results ambiguous in determination of the safety of the drug formulation.Our findings demonstrate limitations of endotoxin detection in micellar drugs,and call for the necessity of developing reliable endotoxin detection methods that can overcome the interference of nanomaterials in order to better ensure the drug safety of patients in future pharmaceutical drug development.展开更多
Two-dimensional graphene film exhibits sluggish ion diffusivity while three-dimensional(3D)graphene aerogel has low packing density and poor mechanical flexibility.Consequently,there is an urgent need for graphenebase...Two-dimensional graphene film exhibits sluggish ion diffusivity while three-dimensional(3D)graphene aerogel has low packing density and poor mechanical flexibility.Consequently,there is an urgent need for graphenebased film with both mechanical robustness and high specific capacitance.Here,we present an easy and scalable strategy for fabricating a free-standing flexible graphene-based aerogel film electrode with a two-layered structure,in which the top layer is an interconnected macroporous reduced graphene oxide/carbon nanotube(RGO/CNT)aerogel,and the bottom layer is a flexible electrospun polyacrylonitrile(PAN)nanofiber membrane.The porous 3D structure of the aerogel provides fast transport of electrolyte ions and electrons,while the nanofiber membrane provides both strong support for the aerogel and mechanical flexibility.Polypyrrole(PPy)can be uniformly loaded on RGO/CNT/PAN(RCP)composite aerogel film to provide pseudocapacitance,and nitrogen-doped RGO/CNT/carbon nanofiber(NRCC)aerogel film can be obtained by further pyrolysis.The resultant RCP@PPy-0.05//NRCC based asymmetric supercapacitor can have a maximum voltage of 1.7 V and a maximum energy density of 60.6 W h kg^(-1)at 850.2 W kg^(-1).This indicates that free-standing graphene-based aerogel film can be used in flexible supercapacitors.展开更多
Object:Explore the application and actual effect of MET(Muscle Energy)technology after breast cancer surgery with upper limb dysfunction.Methods:Taking 40 female breast cancer patients who underwent surgical treatment...Object:Explore the application and actual effect of MET(Muscle Energy)technology after breast cancer surgery with upper limb dysfunction.Methods:Taking 40 female breast cancer patients who underwent surgical treatment in our hospital from September 2017 to June 2019 as the research objects,all of them successfully completed modified radical mastectomy for breast cancer.According to different nursing methods,the patients were randomly divided into two groups.The experiment There were 20 cases in each group and the control group.The control group was given routine functional recovery exercise intervention after the operation,and the experimental group added MET technology to the base of the control group.One month after the operation,the functional recovery of the affected limbs of the two groups of patients was effectively assessed.The upper limb dysfunction of the two groups was compared by statistical methods,and the shoulder joint range of motion(ROM)was used for performance.Results:Through early functional recovery training and MET technology,19 cases of ROM in the experimental group showed compliance(95%),compared with only 14 cases(70%)in the control group.The difference in upper limb dysfunction between the two groups is very obvious with statistical significance(P<0.05).Conclusions:Early functional recovery training combined with muscle energy technology can promote the recovery of upper limb dysfunction after breast cancer surgery faster and better,which is conducive to the recovery of patients as soon as possible and improve the quality of life.展开更多
基金supported by the National Natural Science Foundation of China [Grant No. 31600812]Strategic Priority Research Program of the Chinese Academy of Sciences [Grant No. XDA09030301]+1 种基金Natural Science Foundation Key Project [Grant No. 31630027, 31430031]the National Distinguished Young Scholars Grant [Grant No. 31225009]
文摘Endotoxin detection is an important step in drug characterization. Herein we found that a chemotherapeutic drug nanoformulation composed of irinotecan hydrochloride(CPT-11) and an amphiphilic molecule DSPE-mPEG_(2000) can interfere with the limulus amebocyte lysate assay(LAL). Furthermore, the rabbit pyrogen test(RPT) results indicated that at a relatively high dosage, the drug irinotecan hydrochloride can induce a hypothermia effect which may render the RPT results ambiguous in determination of the safety of the drug formulation.Our findings demonstrate limitations of endotoxin detection in micellar drugs,and call for the necessity of developing reliable endotoxin detection methods that can overcome the interference of nanomaterials in order to better ensure the drug safety of patients in future pharmaceutical drug development.
基金the financial support from the National Natural Science Foundation of China(21704014,21674019)Fundamental Research Funds for Central Universities(2232019A3-03)+2 种基金the Program of Shanghai Academic Research Leader(17XD1400100)the Shanghai Sailing Program(17YF1400200)the Shanghai Municipal Education Commission(17CG33).
文摘Two-dimensional graphene film exhibits sluggish ion diffusivity while three-dimensional(3D)graphene aerogel has low packing density and poor mechanical flexibility.Consequently,there is an urgent need for graphenebased film with both mechanical robustness and high specific capacitance.Here,we present an easy and scalable strategy for fabricating a free-standing flexible graphene-based aerogel film electrode with a two-layered structure,in which the top layer is an interconnected macroporous reduced graphene oxide/carbon nanotube(RGO/CNT)aerogel,and the bottom layer is a flexible electrospun polyacrylonitrile(PAN)nanofiber membrane.The porous 3D structure of the aerogel provides fast transport of electrolyte ions and electrons,while the nanofiber membrane provides both strong support for the aerogel and mechanical flexibility.Polypyrrole(PPy)can be uniformly loaded on RGO/CNT/PAN(RCP)composite aerogel film to provide pseudocapacitance,and nitrogen-doped RGO/CNT/carbon nanofiber(NRCC)aerogel film can be obtained by further pyrolysis.The resultant RCP@PPy-0.05//NRCC based asymmetric supercapacitor can have a maximum voltage of 1.7 V and a maximum energy density of 60.6 W h kg^(-1)at 850.2 W kg^(-1).This indicates that free-standing graphene-based aerogel film can be used in flexible supercapacitors.
基金Item Number:2018020778 project name:Clinical study of MET technique on the improvement of lymphedema and shoulder dysfunction after breast cancer surgery.Item Number:2019ZY3011 project name:Index analysis of the influence of Weisan acupuncture on sarcopenia.Item Number:LHGJ20191057 project name:JAK/STAT signal regulation of macrophages involved in tendon remodeling and its mechanism.
文摘Object:Explore the application and actual effect of MET(Muscle Energy)technology after breast cancer surgery with upper limb dysfunction.Methods:Taking 40 female breast cancer patients who underwent surgical treatment in our hospital from September 2017 to June 2019 as the research objects,all of them successfully completed modified radical mastectomy for breast cancer.According to different nursing methods,the patients were randomly divided into two groups.The experiment There were 20 cases in each group and the control group.The control group was given routine functional recovery exercise intervention after the operation,and the experimental group added MET technology to the base of the control group.One month after the operation,the functional recovery of the affected limbs of the two groups of patients was effectively assessed.The upper limb dysfunction of the two groups was compared by statistical methods,and the shoulder joint range of motion(ROM)was used for performance.Results:Through early functional recovery training and MET technology,19 cases of ROM in the experimental group showed compliance(95%),compared with only 14 cases(70%)in the control group.The difference in upper limb dysfunction between the two groups is very obvious with statistical significance(P<0.05).Conclusions:Early functional recovery training combined with muscle energy technology can promote the recovery of upper limb dysfunction after breast cancer surgery faster and better,which is conducive to the recovery of patients as soon as possible and improve the quality of life.
