Filtering capacitor with compact configuration and a wide range of operating voltage has been attracting increasing attention for the smooth conversion of the electric signal in modern circuits.Lossless integration of...Filtering capacitor with compact configuration and a wide range of operating voltage has been attracting increasing attention for the smooth conversion of the electric signal in modern circuits.Lossless integration of capacitor units can be regarded as one of the efficient ways to achieve a wider voltage range,which has not yet been fully conquered due to the lack of rational designs of the electrode structure and integration technology.This study presents an alternatingly stacked assemble technology to conveniently fabricate compact aqueous hybrid integrated filtering capacitors on a large scale,in which a unit consists of rGO/MXene composite film as a negative electrode and PEDOT:PSS based film as a positive electrode.Benefiting from the synergistic effect of rGO and MXene components,and morphological characteristics of PEDOT:PSS,the capacitor unit exhibits outstanding AC line filtering with a large areal specific energy density of 1,015 μF V^(2)cm^(-2)(0.28 μW h cm^(-2)) at 120 Hz.After rational integration,the assembled capacitors present compact/lightweight configuration and lossless frequency response,as reflected by almost constant resistor-capacitor time constant of 0.2 ms and dissipation factor of 15% at120 Hz,identical to those of the single capacitor unit.Apart from standing alone steadily on a flower,a small volume(only 8.1 cm^(3)) of the integrated capacitor with 70 units connected in series achieves hundred-volts alternating current line filtering,which is superior to most reported filtering capacitors with sandwich configuration.This study provides insight into the fabrication and application of compact/ultralight filtering capacitors with lossless frequency response,and a wide range of operating voltage.展开更多
[Objectives]To explore the effects and mechanism of miR-15a-5p on oxaliplatin resistance in colorectal cancer HCT116/L cells.[Methods]The expression of miR-15a-5p in colorectal cancer sensitive cells HCT116 and resist...[Objectives]To explore the effects and mechanism of miR-15a-5p on oxaliplatin resistance in colorectal cancer HCT116/L cells.[Methods]The expression of miR-15a-5p in colorectal cancer sensitive cells HCT116 and resistant cells HCT116/L was detected by RT-qPCR method;the effect of oxaliplatin on the proliferation of HCT116 and HCT116/L cells was detected by MTT,and its IC_(50) and drug resistance fold of HCT116/L cells were calculated;the expressions of Wnt3a,β-Catenin and P-gp in HCT116 and HCT116/L cells were detected by Western Blot method.HCT-116/L cells were divided into 5 groups:blank control group HCT116/L,control group 1 transfected with miR-15a-5p mimics NC,experimental group 1 transfected with miR-15a-5p mimics,control group 2 transfected with miR-15a-5p inhibitor NC,and experimental group 2 transfected with miR-15a-5p inhibitor.The expression of miR-15a-5p in each group was detected by RT-qPCR method and the transfection efficiency was detected.The effect of different concentrations of oxaliplatin on the proliferation of cells in each group after transfection was detected by MTT and the half inhibitory concentration(50%inhibiting concentration,IC_(50))was calculated.The expressions of Wnt3a,β-catenin and P-gp in each group after transfection were detected by Western Blot method,and the expressions of Wnt3a,β-catenin and MDR1 mRNA in each group after transfection were detected by RT-qPCR method.[Results](i)RT-qPCR results showed that the expression of miR-15a-5p in HCT116/L cells was(0.16±0.05)significantly lower than that in HCT116 cells(P<0.05).(ii)The IC50 of oxaliplatin for HCT-116 cells and HCT116/L cells detected by MTT method were(13.51±2.62)and(103.08±12.29)μg/mL,respectively.The drug resistance index of HCT116/L was 7.63.(iii)Western Blot results showed that the expressions of Wnt3a,β-catenin and P-gp in HCT116/L cells were significantly higher than those in HCT-116 cells(P<0.05).(iv)After successful transfection,the IC50 of miR-15a-5p mimics group to oxaliplatin decreased to(40.78±2.47)μg/mL by MTT method,and its sensitivity to the drug was significantly improved compared with the control group.Western Blot results showed that the relative expressions of P-gp,Wnt3a andβ-catenin were significantly down-regulated(all P<0.05);RT-qPCR results showed that the relative expressions of MDR1,Wnt3a andβ-catenin mRNA were significantly down-regulated(all P<0.05).(v)After successful transfection,the expression of miR-15a-5p in the miR-15a-5p inhibitor transfection group was(0.38±0.04);MTT results showed that its IC50 for oxaliplatin was up-regulated to(132.77±7.97)μg/mL,and its sensitivity to chemotherapy drugs was significantly lower than that of the control group;Western Blot results showed that the relative expressions of P-gp,Wnt3a andβ-catenin were significantly up-regulated(all P<0.05);RT-qPCR results showed that the relative expressions of MDR1,Wnt3a andβ-catenin mRNA were significantly up-regulated(all P<0.05).[Conclusions]Up-regulation of miR-15a-5p expression can reverse the resistance of HCT116/L cell line to oxaliplatin.Up-regulation or down-regulation of miR-15a-5p will affect the expression of P-gp and Wnt/β-catenin signaling pathway-related proteins,suggesting that the mechanism of miR-15a-5p reversal of drug resistance may be related to the inhibition of Wnt/β-catenin pathway,thereby down-regulating the expression of P-gp.展开更多
Nanocatalytic medicine triggering in situ catalytic reactions has been considered as a promising strategy for tumor-selective therapeutics.However,the targeted distribution of nanocatalysts was still low,considering t...Nanocatalytic medicine triggering in situ catalytic reactions has been considered as a promising strategy for tumor-selective therapeutics.However,the targeted distribution of nanocatalysts was still low,considering the absence of targeting propulsion capability.Here,encouraged by the fast-developing controllable microrobotics for targeting delivery,a sunflower-like nanocatalytic active swarm(SNCAS)controlled by a three-dimensional(3D)magnetic field was proposed for synergistic tumorselective and magnetic-actively tumor-targeting therapeutics.Furthermore,a patient-derived renal cancer cell 3D organoid was utilized for the verification of the effective tumor therapeutic outcomes.Under the targeted control of 3D magnetic field,the multiple cascade catalytic efficiency of SNCAS based on Fenton reaction was evaluated,resulting in efficient tumor cell apoptosis and death.For the patient-derived organoid treatment,the SNCAS presented significant lethality toward 3D organoid structure to induce cell apoptosis with the collapse of organoid morphology.The targeting efficiency was further enhanced under the magnetic-controllable of SNCAS.Overall,empowered by the magnetic control technology,the synergistic therapeutic strategy based on controllable swarm combined active targeting and tumor-specific catalytic nanomedicine has provided a novel way for advanced cancer therapy.Meanwhile,3D patient-derived organoids were proved as a powerful tool for the effectiveness verification of nanocatalytic medicine.展开更多
Globally, SARS-CoV-2 has infected 3,113,447 people and killed 216,930 as of April 29, 2020. Identifying populations vulnerable to infection and their disease progression is critical to mitigating the negative impacts ...Globally, SARS-CoV-2 has infected 3,113,447 people and killed 216,930 as of April 29, 2020. Identifying populations vulnerable to infection and their disease progression is critical to mitigating the negative impacts on healthcare systems. Recent studies have shown that angiotensin converting enzyme 2 (ACE2) is the receptor for SARS-CoV-2 to enter human cells (Zhou et al., 2020), raising the possibility that a higher ACE2 expression level could facilitate SARS-CoV-2infection.展开更多
In this work, 16 kinds of [FeCl4]--based magnetic ionic liquids （ILs） with different cation structures have been designed and synthesized, and their structures are characterized by IR and Raman spectroscopy. Then th...In this work, 16 kinds of [FeCl4]--based magnetic ionic liquids （ILs） with different cation structures have been designed and synthesized, and their structures are characterized by IR and Raman spectroscopy. Then the lower critical solution temperature （LCST）-type phase behavior of these magnetic ILs in water is investigated as a function of concentration. It is shown that cat- ion structure, alkyl chain length and molar ratio of FeCl3/chloride IL have a significant influence on the LCST of the mixtures. The phase separation temperature can be tuned efficiently by these factors. Meanwhile, the LCST-type phase separation pro- cess is also investigated by dynamic light scattering. The results support the mechanism that the hydrogen bonds of the [Fefl4]- anion with water have been gradually disrupted to form ILs aggregates with increasing temperature. In addition, the stability of the ILs in water is also examined in some details. These LCST-type phase separation systems may have potential applications in extraction and separation techniques at room temperature.展开更多
基金supported by the NSFC(21805072,22075019,22035005)the National Key R&D Program of China(2017YFB1104300)。
文摘Filtering capacitor with compact configuration and a wide range of operating voltage has been attracting increasing attention for the smooth conversion of the electric signal in modern circuits.Lossless integration of capacitor units can be regarded as one of the efficient ways to achieve a wider voltage range,which has not yet been fully conquered due to the lack of rational designs of the electrode structure and integration technology.This study presents an alternatingly stacked assemble technology to conveniently fabricate compact aqueous hybrid integrated filtering capacitors on a large scale,in which a unit consists of rGO/MXene composite film as a negative electrode and PEDOT:PSS based film as a positive electrode.Benefiting from the synergistic effect of rGO and MXene components,and morphological characteristics of PEDOT:PSS,the capacitor unit exhibits outstanding AC line filtering with a large areal specific energy density of 1,015 μF V^(2)cm^(-2)(0.28 μW h cm^(-2)) at 120 Hz.After rational integration,the assembled capacitors present compact/lightweight configuration and lossless frequency response,as reflected by almost constant resistor-capacitor time constant of 0.2 ms and dissipation factor of 15% at120 Hz,identical to those of the single capacitor unit.Apart from standing alone steadily on a flower,a small volume(only 8.1 cm^(3)) of the integrated capacitor with 70 units connected in series achieves hundred-volts alternating current line filtering,which is superior to most reported filtering capacitors with sandwich configuration.This study provides insight into the fabrication and application of compact/ultralight filtering capacitors with lossless frequency response,and a wide range of operating voltage.
文摘[Objectives]To explore the effects and mechanism of miR-15a-5p on oxaliplatin resistance in colorectal cancer HCT116/L cells.[Methods]The expression of miR-15a-5p in colorectal cancer sensitive cells HCT116 and resistant cells HCT116/L was detected by RT-qPCR method;the effect of oxaliplatin on the proliferation of HCT116 and HCT116/L cells was detected by MTT,and its IC_(50) and drug resistance fold of HCT116/L cells were calculated;the expressions of Wnt3a,β-Catenin and P-gp in HCT116 and HCT116/L cells were detected by Western Blot method.HCT-116/L cells were divided into 5 groups:blank control group HCT116/L,control group 1 transfected with miR-15a-5p mimics NC,experimental group 1 transfected with miR-15a-5p mimics,control group 2 transfected with miR-15a-5p inhibitor NC,and experimental group 2 transfected with miR-15a-5p inhibitor.The expression of miR-15a-5p in each group was detected by RT-qPCR method and the transfection efficiency was detected.The effect of different concentrations of oxaliplatin on the proliferation of cells in each group after transfection was detected by MTT and the half inhibitory concentration(50%inhibiting concentration,IC_(50))was calculated.The expressions of Wnt3a,β-catenin and P-gp in each group after transfection were detected by Western Blot method,and the expressions of Wnt3a,β-catenin and MDR1 mRNA in each group after transfection were detected by RT-qPCR method.[Results](i)RT-qPCR results showed that the expression of miR-15a-5p in HCT116/L cells was(0.16±0.05)significantly lower than that in HCT116 cells(P<0.05).(ii)The IC50 of oxaliplatin for HCT-116 cells and HCT116/L cells detected by MTT method were(13.51±2.62)and(103.08±12.29)μg/mL,respectively.The drug resistance index of HCT116/L was 7.63.(iii)Western Blot results showed that the expressions of Wnt3a,β-catenin and P-gp in HCT116/L cells were significantly higher than those in HCT-116 cells(P<0.05).(iv)After successful transfection,the IC50 of miR-15a-5p mimics group to oxaliplatin decreased to(40.78±2.47)μg/mL by MTT method,and its sensitivity to the drug was significantly improved compared with the control group.Western Blot results showed that the relative expressions of P-gp,Wnt3a andβ-catenin were significantly down-regulated(all P<0.05);RT-qPCR results showed that the relative expressions of MDR1,Wnt3a andβ-catenin mRNA were significantly down-regulated(all P<0.05).(v)After successful transfection,the expression of miR-15a-5p in the miR-15a-5p inhibitor transfection group was(0.38±0.04);MTT results showed that its IC50 for oxaliplatin was up-regulated to(132.77±7.97)μg/mL,and its sensitivity to chemotherapy drugs was significantly lower than that of the control group;Western Blot results showed that the relative expressions of P-gp,Wnt3a andβ-catenin were significantly up-regulated(all P<0.05);RT-qPCR results showed that the relative expressions of MDR1,Wnt3a andβ-catenin mRNA were significantly up-regulated(all P<0.05).[Conclusions]Up-regulation of miR-15a-5p expression can reverse the resistance of HCT116/L cell line to oxaliplatin.Up-regulation or down-regulation of miR-15a-5p will affect the expression of P-gp and Wnt/β-catenin signaling pathway-related proteins,suggesting that the mechanism of miR-15a-5p reversal of drug resistance may be related to the inhibition of Wnt/β-catenin pathway,thereby down-regulating the expression of P-gp.
基金This work was supported by the National Key R&D Program of China(No.2018YFA0901700)the National Natural Science Foundation of China(Nos.21878173,52175273,and 82072837)+1 种基金the 111 Project(No.B17026)a grant from the Institute Guo Qiang,Tsinghua University(No.2021GQG1016).
文摘Nanocatalytic medicine triggering in situ catalytic reactions has been considered as a promising strategy for tumor-selective therapeutics.However,the targeted distribution of nanocatalysts was still low,considering the absence of targeting propulsion capability.Here,encouraged by the fast-developing controllable microrobotics for targeting delivery,a sunflower-like nanocatalytic active swarm(SNCAS)controlled by a three-dimensional(3D)magnetic field was proposed for synergistic tumorselective and magnetic-actively tumor-targeting therapeutics.Furthermore,a patient-derived renal cancer cell 3D organoid was utilized for the verification of the effective tumor therapeutic outcomes.Under the targeted control of 3D magnetic field,the multiple cascade catalytic efficiency of SNCAS based on Fenton reaction was evaluated,resulting in efficient tumor cell apoptosis and death.For the patient-derived organoid treatment,the SNCAS presented significant lethality toward 3D organoid structure to induce cell apoptosis with the collapse of organoid morphology.The targeting efficiency was further enhanced under the magnetic-controllable of SNCAS.Overall,empowered by the magnetic control technology,the synergistic therapeutic strategy based on controllable swarm combined active targeting and tumor-specific catalytic nanomedicine has provided a novel way for advanced cancer therapy.Meanwhile,3D patient-derived organoids were proved as a powerful tool for the effectiveness verification of nanocatalytic medicine.
基金supported by the National Key R&D Program of China (2018YFA0507503)。
文摘Globally, SARS-CoV-2 has infected 3,113,447 people and killed 216,930 as of April 29, 2020. Identifying populations vulnerable to infection and their disease progression is critical to mitigating the negative impacts on healthcare systems. Recent studies have shown that angiotensin converting enzyme 2 (ACE2) is the receptor for SARS-CoV-2 to enter human cells (Zhou et al., 2020), raising the possibility that a higher ACE2 expression level could facilitate SARS-CoV-2infection.
基金supported by the National Natural Science Foundation of China (21473050, 21403060)Program for Innovative Research Team in Science and Technology in University of Henan Province (16IRTSTHN002)+1 种基金Plan for Scientific Innovation Talent of Henan Province of China (144200510004)the Natural Science Research Program of Henan Educational Committee (2010A150014)
文摘In this work, 16 kinds of [FeCl4]--based magnetic ionic liquids （ILs） with different cation structures have been designed and synthesized, and their structures are characterized by IR and Raman spectroscopy. Then the lower critical solution temperature （LCST）-type phase behavior of these magnetic ILs in water is investigated as a function of concentration. It is shown that cat- ion structure, alkyl chain length and molar ratio of FeCl3/chloride IL have a significant influence on the LCST of the mixtures. The phase separation temperature can be tuned efficiently by these factors. Meanwhile, the LCST-type phase separation pro- cess is also investigated by dynamic light scattering. The results support the mechanism that the hydrogen bonds of the [Fefl4]- anion with water have been gradually disrupted to form ILs aggregates with increasing temperature. In addition, the stability of the ILs in water is also examined in some details. These LCST-type phase separation systems may have potential applications in extraction and separation techniques at room temperature.
