BACKGROUND： Neonatal cerebral palsy is mainly caused by prenatal factors. At present, an animal model of prenatal infection and early postnatal hypoxia does not exist. OBJECTIVE： To observe morphology and motor perf...BACKGROUND： Neonatal cerebral palsy is mainly caused by prenatal factors. At present, an animal model of prenatal infection and early postnatal hypoxia does not exist. OBJECTIVE： To observe morphology and motor performance following prenatal infection and hypoxic insult-induced brain damage of neonatal rats to verify the feasibility to establish a model of cerebral palsy. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratories of Xinjiang Center for Disease Control and Prevention from September 2007 to June 2008. MATERIALS： The hypoxic incubator was purchased from Shanghai Pediatric Medical Institute, China. Lipopolysaccharide （LPS, Escherichia coil, 055： B5） was purchased from Sigma-Aldrich （St. Louis, MO, USA）. METHODS： A total of 27 Wistar rats, aged 7 days, were randomly assigned to sham-surgery group （n = 15） with no carotid artery incision or hypoxia treatment, hypoxia/ischemia （H/I） group （n = 12） undergoing ligature of the right common carotid artery followed by exposure to hypoxia at postnatal day 7 （P7）, and LPS/H group （n = 19）, in which pregnant rats were exposed in utero to LPS followed by prenatal hypoxia at embryonic day 16. MAIN OUTCOME MEASURES： Behavior, compound muscle action potential, and pathological changes were observed in 28-day-old rats. RESULTS： The footprint repeat space showed that left limb footprint repeatability in the H/I and LPS/H groups was lower than in the sham-surgery group （P 〈 0.05）. The space between the footprints was larger and unstable. Hind limb quadricep compound muscle action potential in the H/I and LPS/H groups showed lower wave amplitude compared with the sham-surgery group （P〈 0.05） Hematoxylin and eosin staining showed irregular cells around the ventricle, as well as periventricular leukomalacia. CONCLUSION： An animal model of cerebral palsy was established, which simulated the human condition most likely associated with occurrence of this disease. This model could be used for experimental studies related to cerebral palsy.展开更多
Animal models of cerebral palsy established by simple infection or the hypoxia/ischemia method cannot effectively simulate the brain injury of a premature infant. Healthy 17-day-pregnant Wistar rats were intraperitone...Animal models of cerebral palsy established by simple infection or the hypoxia/ischemia method cannot effectively simulate the brain injury of a premature infant. Healthy 17-day-pregnant Wistar rats were intraperitoneally injected with lipopolysaccharide then subjected to hypoxia. The pups were used for this study at 4 weeks of age. Simultaneously, a hypoxia/ischemia group and a control group were used for comparison. The results of the footprint test, the balance beam test, the water maze test, neuroelectrophysiological examination and neuropathological examination demonstrated that, at 4 weeks after birth, footprint repeat space became larger between the forelimbs and hindlimbs of the rats, the latency period on the balance beam and in the Morris water maze was longer, place navigation and ability were poorer, and the stimulus intensity that induced the maximal wave amplitude of the compound muscle action potential was greater in the lipopolysaccharide/hypoxia and hypoxia/ischemia groups than in the control group. We observed irregular cells around the periventricular area, periventricular leukomalacia and breakage of the nuclear membrane in the lipopolysacchadde/hypexia and hypoxia/ischemia groups. These results indicate that we successfully established a Wistar rat pup model of cerebral palsy by intraperitoneal injection of lipopolysaccharide and hypoxia.展开更多
1 Introduction Financial evaluation focuses on pursuing sustainable financial development under the“New Normal”of China’s economy.It is of great practical significance to explore innovative methods based on establi...1 Introduction Financial evaluation focuses on pursuing sustainable financial development under the“New Normal”of China’s economy.It is of great practical significance to explore innovative methods based on established financial evaluation indexes and systems[1,2].The joint strategy of PCA methods and Deep Learning models seems to have a scientific basis and a good fusion mechanism in earlier studies[3-5].The innovation of our research is to combine evaluation method,unsupervised learning,with supervised learning.Based on the PCA method,the selected data is dimensionally reduced and comprehensively scored,then the unsupervised learning K-means clustering method is used to divide the sample into five evaluation levels according to the performance score.Finally,combined with the Convolutional Neural Network(CNN)framework,the company’s financial performance can be profiled and predicted.On one hand,the theoretical significance of this study is to combine the traditional PCA method with the Deep Learning method to predict the performance level for the performance portrait of enterprises.展开更多
基金the National Natural Science Foundation of China,No.30960393Key Foundation in Science and Technology of Xinjiang Uygur Autonomous Region,No.200633128(2)the Youth Science and Technology Foundation of Health Department of Xinjiang Uygur Autonomous Region,No.2007Y26
文摘BACKGROUND： Neonatal cerebral palsy is mainly caused by prenatal factors. At present, an animal model of prenatal infection and early postnatal hypoxia does not exist. OBJECTIVE： To observe morphology and motor performance following prenatal infection and hypoxic insult-induced brain damage of neonatal rats to verify the feasibility to establish a model of cerebral palsy. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratories of Xinjiang Center for Disease Control and Prevention from September 2007 to June 2008. MATERIALS： The hypoxic incubator was purchased from Shanghai Pediatric Medical Institute, China. Lipopolysaccharide （LPS, Escherichia coil, 055： B5） was purchased from Sigma-Aldrich （St. Louis, MO, USA）. METHODS： A total of 27 Wistar rats, aged 7 days, were randomly assigned to sham-surgery group （n = 15） with no carotid artery incision or hypoxia treatment, hypoxia/ischemia （H/I） group （n = 12） undergoing ligature of the right common carotid artery followed by exposure to hypoxia at postnatal day 7 （P7）, and LPS/H group （n = 19）, in which pregnant rats were exposed in utero to LPS followed by prenatal hypoxia at embryonic day 16. MAIN OUTCOME MEASURES： Behavior, compound muscle action potential, and pathological changes were observed in 28-day-old rats. RESULTS： The footprint repeat space showed that left limb footprint repeatability in the H/I and LPS/H groups was lower than in the sham-surgery group （P 〈 0.05）. The space between the footprints was larger and unstable. Hind limb quadricep compound muscle action potential in the H/I and LPS/H groups showed lower wave amplitude compared with the sham-surgery group （P〈 0.05） Hematoxylin and eosin staining showed irregular cells around the ventricle, as well as periventricular leukomalacia. CONCLUSION： An animal model of cerebral palsy was established, which simulated the human condition most likely associated with occurrence of this disease. This model could be used for experimental studies related to cerebral palsy.
基金funded by the National Natural Science Foundation of China,No.30960393the Key Foundation in Science and Technology of Xinjiang Uygur Autonomous Region,No.200633128(2)+1 种基金the Youth Science and Technology Foundation of Health Department of Xinjiang Uygur Autonomous Region,No.2007Y26the Science and Technology Foundation of Health Bureau of Wuxi,No.ML201211
文摘Animal models of cerebral palsy established by simple infection or the hypoxia/ischemia method cannot effectively simulate the brain injury of a premature infant. Healthy 17-day-pregnant Wistar rats were intraperitoneally injected with lipopolysaccharide then subjected to hypoxia. The pups were used for this study at 4 weeks of age. Simultaneously, a hypoxia/ischemia group and a control group were used for comparison. The results of the footprint test, the balance beam test, the water maze test, neuroelectrophysiological examination and neuropathological examination demonstrated that, at 4 weeks after birth, footprint repeat space became larger between the forelimbs and hindlimbs of the rats, the latency period on the balance beam and in the Morris water maze was longer, place navigation and ability were poorer, and the stimulus intensity that induced the maximal wave amplitude of the compound muscle action potential was greater in the lipopolysaccharide/hypoxia and hypoxia/ischemia groups than in the control group. We observed irregular cells around the periventricular area, periventricular leukomalacia and breakage of the nuclear membrane in the lipopolysacchadde/hypexia and hypoxia/ischemia groups. These results indicate that we successfully established a Wistar rat pup model of cerebral palsy by intraperitoneal injection of lipopolysaccharide and hypoxia.
基金supported by the Humanity and Social Science Foundation of Ministry of Education of China(Nos.18YJA630037,21YJA630054)Zhejiang Provincial Natural Science Foundation of China(Nos.LY18G010005,LY17G020025)Zhejiang Philosophy and Social Science Program of China(Nos.19NDJC240YB,17NDJC262YB).
文摘1 Introduction Financial evaluation focuses on pursuing sustainable financial development under the“New Normal”of China’s economy.It is of great practical significance to explore innovative methods based on established financial evaluation indexes and systems[1,2].The joint strategy of PCA methods and Deep Learning models seems to have a scientific basis and a good fusion mechanism in earlier studies[3-5].The innovation of our research is to combine evaluation method,unsupervised learning,with supervised learning.Based on the PCA method,the selected data is dimensionally reduced and comprehensively scored,then the unsupervised learning K-means clustering method is used to divide the sample into five evaluation levels according to the performance score.Finally,combined with the Convolutional Neural Network(CNN)framework,the company’s financial performance can be profiled and predicted.On one hand,the theoretical significance of this study is to combine the traditional PCA method with the Deep Learning method to predict the performance level for the performance portrait of enterprises.
