The recycling and remanufacturing of end-of-life products are significant for environmental protection and resource conservation.Disassembly is an essential process of remanufacturing end-of-life products.Effective di...The recycling and remanufacturing of end-of-life products are significant for environmental protection and resource conservation.Disassembly is an essential process of remanufacturing end-of-life products.Effective disassembly plans help improve disassembly efficiency and reduce disassembly costs.This paper studies a disassembly planning problem with operation attributes,in which an integrated decision of the disassembly sequence,disassembly directions,and disassembly tools are made.Besides,a mathematical model is formulated with the objective of minimizing the penalty cost caused by the changing of operation attributes.Then,a neighborhood modularization-based artificial bee colony algorithm is developed,which contains a modular optimized design.Finally,two case studies with different scales and complexities are used to verify the performance of the proposed approach,and experimental results show that the proposed algorithm outperforms the two existing methods within an acceptable computational time.展开更多
White blood cells(WBCs)play essential roles against inflammatory disorders,bacterial infections,and cancers.Inspired by nature,WBC membrane-camouflaged nanocarriers(WBC-NCs)have been developed to mimic the“dynamic”f...White blood cells(WBCs)play essential roles against inflammatory disorders,bacterial infections,and cancers.Inspired by nature,WBC membrane-camouflaged nanocarriers(WBC-NCs)have been developed to mimic the“dynamic”functions of WBCs,such as transendothelial migration,adhesion to injured blood vessels,etc,which make them promising for diverse medical applications.WBC-NCs inherit the cell membrane antigens of WBCs,while still exhibiting the robust inflammation-related therapeutic potential of synthetic nanocarriers with excellent(bio)physicochemical performance.This review summarizes the proposed concept of cell membrane engineering,which utilizes physical engineering,chemical modification,and biological functionalization technologies to endow the natural cell membrane with abundant functionalities.In addition,it highlights the recent progress and applications of WBC-NCs for inflammation targeting,biological neutralization,and immune modulation.Finally,the challenges and opportunities in realizing the full potential of WBC-NCs for the manipulation of inflammation-related therapeutics are discussed.展开更多
In recent years,the use of convolutional neural networks(CNNs)and graph neural networks(GNNs)to identify hyperspectral images(HSIs)has achieved excellent results,and such methods are widely used in agricultural remote...In recent years,the use of convolutional neural networks(CNNs)and graph neural networks(GNNs)to identify hyperspectral images(HSIs)has achieved excellent results,and such methods are widely used in agricultural remote sensing,geological exploration,and marine remote sensing.Although many generalization classification algorithms are designed for the purpose of learning a small number of samples,there is often a problem of a low utilization rate of position information in the empty spectral domain.Based on this,a GNN with an autoregressive moving average(ARMA)-based smoothingfilter samples the node information in the null spectral domain and then captures the spatial information at the pixel level via spatial feature convolution;then,the null spectral domain position information lost by the CNN is located by a coordinate attention(CA)mechanism.Finally,autoregressive,spatial convolution,and CA mechanisms are combined into multiscale features to enhance the learning capacity of the network for tiny samples.Experiments conducted on the widely used Indian Pines(IP)dataset,the Botswana(BS)dataset,Houton 2013(H2013),and the WHU-Hi-HongHu(WHU)benchmark HSI dataset demonstrate that the proposed GACP technique can perform classification work with good accuracy even with a small number of training examples.展开更多
During the establishment of rhizobia-legume symbiosis, the cytokinin receptor LHK1(Lotus Histidine Kinase 1) is essential for nodule formation. However,the mechanism by which cytokinin signaling regulates symbiosis re...During the establishment of rhizobia-legume symbiosis, the cytokinin receptor LHK1(Lotus Histidine Kinase 1) is essential for nodule formation. However,the mechanism by which cytokinin signaling regulates symbiosis remains largely unknown. In this study, an LHK1-interacting protein, LjCZF1, was identified and further characterized. LjCZF1 is a C3HC4-type RING finger protein that is highly conserved in plants. LjCZF1 specifically interacted with LHK1 in yeast two-hybrid, in vitro pull-down and co-immunoprecipitation assays conducted in tobacco. Phosphomimetic mutation of the potential threonine(T167D) phosphorylation site enhanced the interaction between LjCZF1 and LHK1,whereas phosphorylation mutation(T167 A) eliminated this interaction. Transcript abundance of LjCZF1 was upregulated significantly after inoculation with rhizobia. The LORE1 insertion mutant and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPRassociated protein 9-mediated knockout mutant Lotus japonicus plants demonstrated significantly reduced number of infection threads and nodules. In contrast,plants over-expressing LjCZF1 exhibited increased numbers of infection threads and nodules. Collectively, these data support the notion that LjCZF1 is a positive regulator of symbiotic nodulation, possibly through interaction with LHK1.展开更多
基金National Natural Science Foundation of China(Grant Nos.52205526,52205529)Basic and Applied Basic Research Project of the Guangzhou Basic Research Program of China(Grant No.202201010284)+6 种基金National Foreign Expert Project of the Ministry of Science and Technology of China(Grant No.G2021199026L)National Key Research and Development Program of China(Grant Nos.2021YFB3301701,2021YFB3301702)Guangdong Provincial Graduate Education Innovation Program of China(Grant No.82620516)Guangzhou Municipal Innovation Leading Team Project of China(Grant No.201909010006)Guangdong Provincial"Quality Engineering"Construction Project of China(Grant No.210308)Guangdong Provincial Basic and Applied Basic Research Foundation of China(Grant No.2019A1515110399)Fundamental Research Funds for the Central Universities of China(Grant No.21620360).
文摘The recycling and remanufacturing of end-of-life products are significant for environmental protection and resource conservation.Disassembly is an essential process of remanufacturing end-of-life products.Effective disassembly plans help improve disassembly efficiency and reduce disassembly costs.This paper studies a disassembly planning problem with operation attributes,in which an integrated decision of the disassembly sequence,disassembly directions,and disassembly tools are made.Besides,a mathematical model is formulated with the objective of minimizing the penalty cost caused by the changing of operation attributes.Then,a neighborhood modularization-based artificial bee colony algorithm is developed,which contains a modular optimized design.Finally,two case studies with different scales and complexities are used to verify the performance of the proposed approach,and experimental results show that the proposed algorithm outperforms the two existing methods within an acceptable computational time.
基金The authors are very grateful for the financial support by National Natural Science Foundation of China(Grant No.31900957)Shandong Provincial Natural Science Foundation(Grant No.ZR2019QC007)+4 种基金Innovation and technology program for the excellent youth scholars of higher education of Shandong province(Grant No.2019KJE015)Traditional Chinese Medicine Science and Technology Project of Shandong province(Grant No.2021Q069)Open Fund of Tianjin Enterprise Key Laboratory for Application Research of Hyaluronic Acid(Grant No.KTRDHA-Y201902)National College Students Innovation and Entrepreneurship Training Program of China(Grant No.S202011065041,202211065038)Zhejiang Engineering Research Center for Tissue Repair Materials(Grant No:WIUCASZZXF21004).
文摘White blood cells(WBCs)play essential roles against inflammatory disorders,bacterial infections,and cancers.Inspired by nature,WBC membrane-camouflaged nanocarriers(WBC-NCs)have been developed to mimic the“dynamic”functions of WBCs,such as transendothelial migration,adhesion to injured blood vessels,etc,which make them promising for diverse medical applications.WBC-NCs inherit the cell membrane antigens of WBCs,while still exhibiting the robust inflammation-related therapeutic potential of synthetic nanocarriers with excellent(bio)physicochemical performance.This review summarizes the proposed concept of cell membrane engineering,which utilizes physical engineering,chemical modification,and biological functionalization technologies to endow the natural cell membrane with abundant functionalities.In addition,it highlights the recent progress and applications of WBC-NCs for inflammation targeting,biological neutralization,and immune modulation.Finally,the challenges and opportunities in realizing the full potential of WBC-NCs for the manipulation of inflammation-related therapeutics are discussed.
基金supported by National Natural Science Foundation of China(No.62166005)National Key R&D Program of China(No.2018AAA0101800)+3 种基金Guizhou Provincial Key Technology R&D Program(No.QKH[2022]130,QKH[2022]003,QKH[2021]335)Developing Objects and Projects of Scientific and Technological Talents in Guiyang City(No.ZKHT[2023]48-8)Joint Open Fund Project of Key Laboratories of the Ministry of Education([2020]245,[2020]248)Foundation of State Key Laboratory of Public Big Data(No.QJJ[2022]418).
文摘In recent years,the use of convolutional neural networks(CNNs)and graph neural networks(GNNs)to identify hyperspectral images(HSIs)has achieved excellent results,and such methods are widely used in agricultural remote sensing,geological exploration,and marine remote sensing.Although many generalization classification algorithms are designed for the purpose of learning a small number of samples,there is often a problem of a low utilization rate of position information in the empty spectral domain.Based on this,a GNN with an autoregressive moving average(ARMA)-based smoothingfilter samples the node information in the null spectral domain and then captures the spatial information at the pixel level via spatial feature convolution;then,the null spectral domain position information lost by the CNN is located by a coordinate attention(CA)mechanism.Finally,autoregressive,spatial convolution,and CA mechanisms are combined into multiscale features to enhance the learning capacity of the network for tiny samples.Experiments conducted on the widely used Indian Pines(IP)dataset,the Botswana(BS)dataset,Houton 2013(H2013),and the WHU-Hi-HongHu(WHU)benchmark HSI dataset demonstrate that the proposed GACP technique can perform classification work with good accuracy even with a small number of training examples.
基金supported by the National Natural Science Foundation of China(31370278)the National Basic Research Program of China(973 Program 2010CB126502)the Fundamental Research Funds for the Central Universities(2662015PY171)
文摘During the establishment of rhizobia-legume symbiosis, the cytokinin receptor LHK1(Lotus Histidine Kinase 1) is essential for nodule formation. However,the mechanism by which cytokinin signaling regulates symbiosis remains largely unknown. In this study, an LHK1-interacting protein, LjCZF1, was identified and further characterized. LjCZF1 is a C3HC4-type RING finger protein that is highly conserved in plants. LjCZF1 specifically interacted with LHK1 in yeast two-hybrid, in vitro pull-down and co-immunoprecipitation assays conducted in tobacco. Phosphomimetic mutation of the potential threonine(T167D) phosphorylation site enhanced the interaction between LjCZF1 and LHK1,whereas phosphorylation mutation(T167 A) eliminated this interaction. Transcript abundance of LjCZF1 was upregulated significantly after inoculation with rhizobia. The LORE1 insertion mutant and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPRassociated protein 9-mediated knockout mutant Lotus japonicus plants demonstrated significantly reduced number of infection threads and nodules. In contrast,plants over-expressing LjCZF1 exhibited increased numbers of infection threads and nodules. Collectively, these data support the notion that LjCZF1 is a positive regulator of symbiotic nodulation, possibly through interaction with LHK1.
