AIM: To investigate the incidence of reflux esophagitis (RE) and H pylori infection in the diabetic patient. METHODS: The incidence of RE and H pylori infection were investigated in 85 patients with diabetes mellitus ...AIM: To investigate the incidence of reflux esophagitis (RE) and H pylori infection in the diabetic patient. METHODS: The incidence of RE and H pylori infection were investigated in 85 patients with diabetes mellitus and the results were compared with controls. RESULTS: The incidence of RE in diabetic patients was 17.6%. Although this tended to be higher in diabetic patients, there were no statistically significant differences between diabetic patients and controls. The incidence of H pylori infection in diabetic patients was 53.7% but no statistically significant difference was seen between diabetic patients and controls in the incidence of H pylori infection. CONCLUSION: No significant differences could be seen between diabetic patients and controls in the incidence of RE and H pylori infection.展开更多
AIM:To study the value of serum biomarker tests to differentiate between patients with healthy or diseased stomach mucosa:i.e.those with Helicobacter pylori(H pylori)gastritis or atrophic gastritis,who have a high ris...AIM:To study the value of serum biomarker tests to differentiate between patients with healthy or diseased stomach mucosa:i.e.those with Helicobacter pylori(H pylori)gastritis or atrophic gastritis,who have a high risk of gastric cancer or peptic ulcer diseases.METHODS:Among 162 Japanese outpatients,pepsinogen-(Pg-)and(Pg)were measured using a conventional Japanese technique,and the European GastroPanel examination(Pg and Pg,gastrin-17 and H pylori antibodies).Gastroscopy with gastric biopsies was performed to classify the patients into those with healthy stomach mucosa,H pylori non-atrophic gastritis or atrophic gastritis.RESULTS:Pg-and Pg assays with the GastroPanel and the Japanese method showed a highly significant correlation.For methodological reasons,however,serum Pg-,but not Pg,was twice as high with the GastroPanel test as with the Japanese test.The biomarker assays revealed that 5%of subjects had advanced atrophic corpus gastritis which was also verified by endoscopic biopsies.GastroPanel examination revealed an additional seven patients who had either advanced atrophic gastritis limited to the antrum or antrum-predominant H pylori gastritis.When compared to the endoscopic biopsy findings,the GastroPanel examination classified the patients into groups with "healthy" or "diseased" stomach mucosa with 94% accuracy,95% sensitivity and 93% specifi city.CONCLUSION:Serum biomarker tests can be used to differentiate between subjects with healthy and diseased gastric mucosa with high accuracy.展开更多
AIM: To compare the antisecretory activity and plasma drug concentrations of a single oral dose of 10 mg lafutidine, a novel H2 receptor antagonist, with those of the proton pump inhibitor lansoprazole (LPZ) 30 mg. ME...AIM: To compare the antisecretory activity and plasma drug concentrations of a single oral dose of 10 mg lafutidine, a novel H2 receptor antagonist, with those of the proton pump inhibitor lansoprazole (LPZ) 30 mg. METHODS: Ten volunteers without H pylori infection participated in this crossover study comparing lafutidine 10 mg with LPZ 30 mg. Intragastric pH was monitored for 6 h in all participants, and blood samples were collected from four randomly selected individuals after single-dose administration of each drug. RESULTS: The median intragastric pH was significantly higher in individuals who received lafutidine 10 mg than in those who received LPZ 30 mg 2, 3, 4, 5, and 6 h after administration. Maximal plasma drug concentration was reached more promptly with lafutidine 10 mg than with LPZ 30 mg. CONCLUSION: In H pylori-negative individuals, gastric acid secretion is more markedly inhibited by lafutidinethan by LPZ.展开更多
AIM:To examine the prevalence of gastroesophageal reflux disease (GERD) symptoms in a large unselected general population in Japan. METHODS: In Japan, mature adults are offered regular check-ups for the prevention of ...AIM:To examine the prevalence of gastroesophageal reflux disease (GERD) symptoms in a large unselected general population in Japan. METHODS: In Japan, mature adults are offered regular check-ups for the prevention of gastric cancer. A notice was sent by mail to all inhabitants aged > 40 years. A total of 160 983 Japanese (60 774 male, 100 209 female; mean age 61.9 years) who underwent a stomach check up were enrolled in this study. In addition, from these 160 983 subjects, we randomly selected a total of 82 894 (34 275 male, 48 619 female; mean age 62.4 years) to evaluate the prevalence of abdominal pain. The respective subjects were prospectively asked to complete questionnaires concerning the symptoms of heartburn, dysphagia, and abdominal pain for a 1 mo period. RESULTS: The respective prevalences of the symptoms in males and females were: heartburn, 15.8% vs 20.7%; dysphagia, 5.4% vs 7.8%; and abdominal pain, 6.6% vs 9.6%. Among these symptoms, heartburn was significantly high compared with the other symptoms, and the prevalence of heartburn was significantly more frequent in females than in males in the 60-89-year agegroup. Dysphagia was also significantly more frequent in female patients. CONCLUSION: The prevalence of typical GERD symptoms (heartburn) was high, at about 20% of the Japan population, and the frequency was especially high in females in the 60-89 year age group.展开更多
AIM: To compare rabeprazole (RPZ; 10 mg) with Lansoprazole orally disintegrating tablets (LPZ; 30 mg OD) in terms of antisecretory activity and blood drug concentration after a single dose. METHODS: Eight H pylori-neg...AIM: To compare rabeprazole (RPZ; 10 mg) with Lansoprazole orally disintegrating tablets (LPZ; 30 mg OD) in terms of antisecretory activity and blood drug concentration after a single dose. METHODS: Eight H pylori-negative cytochrome P450 (CYP) 2C19 extensive metabolizers were assigned to receive a single oral dose of RPZ 10 mg or LPZ 30 mg OD. Twelve hour intragastric pH monitoring was perform- ed on the day of treatment. Blood samples were also collected after the administration of each drug. RESULTS: LPZ 30 mg OD induced a significantly earlier rise in blood drug concentration than RPZ 10 mg; consequently, LPZ 30 mg OD induced a significantly earlier rise in median pH in the third and fourth hours of the study. CONCLUSION: In H pylori-negative CYP2C19 extensive metabolizers, LPZ 30 mg OD induced a significantly faster inhibition of gastric acid secretion than RPZ 10 mg.展开更多
AIM:To assess each layer of the optical coherence tomography(OCT)image of the esophageal wall with reference to the histological structure. METHODS:Resected specimens of fresh pig esophagus was used as a model for the...AIM:To assess each layer of the optical coherence tomography(OCT)image of the esophageal wall with reference to the histological structure. METHODS:Resected specimens of fresh pig esophagus was used as a model for the esophageal wall.We injected cyanoacrylate adhesive into the specimens to create a marker,and scanned them using a miniature OCT probe.The localization of these markers was assessed in the OCT images.Then we compared the OCT-imaged morphology with the corresponding histological section,guided by the cyanoacrylate adhesive markers.We prepared a second set of experiments using nylon sutures as markers. RESULTS:The OCT image of the esophageal specimen has a clear five-layered morphology. First,it consisted of a relatively less reflective layer; second,a more reflective layer;third,a less reflective layer;fourth,a more reflective layer;and fifth,a less reflective layer.Comparing the OCT images with marked histological sections showed that the first layer corresponded to stratified squamous epithelium; the second to lamina propria;the third to muscularis mucosa;fourth,submucosa;and fifth,muscularispropria with deeper structures of the esophageal wall.CONCLUSION:We demonstrated that the OCT image of the normal esophageal wall showed a five- layered morphology,which corresponds to histological esophageal wall components.展开更多
AIM: To investigate symptoms and brain activity following esophageal acid infusion. METHODS: Fifteen healthy volunteers were recruited for the study. Hydrochloric acid (pH 1 and 2) and distilled water (pH 7) were rand...AIM: To investigate symptoms and brain activity following esophageal acid infusion. METHODS: Fifteen healthy volunteers were recruited for the study. Hydrochloric acid (pH 1 and 2) and distilled water (pH 7) were randomly and repeatedly infused into the esophagus. The brain activity was evaluated by positron emission tomography. The severity of heartburn elicited by the infusion was rated on an auditory analog scale of 0-10.RESULTS: The severity of heartburn following each infusion showed a step-wise increase with increasing acidity of the perfusate. The heartburn scores were significantly higher in the second pH 1 infusion compared with the first infusion. Acid and distilled water infusion induced activation of various brain areas such as the anterior insula, temporal gyrus, and anterior/ posterior cingulate cortex. At pH 1 or 2, in particular, activation was observed in some emotion-related brain areas such as the more anterior part of the anterior cingulate cortex, parahippocampal gyrus, or the temporal pole. Strong activation of the orbitofrontal cortex was found by subtraction analysis of the two second pH 1 infusions, with a significant increase of heartburn symptoms. CONCLUSION: Emotion-related brain areas were activated by esophageal acid stimulation. The orbitofrontal area might be involved in symptom processing, with esophageal sensitization induced by repeated acid stimulation.展开更多
AIM:To test this hypothesis of barrett esophagus (BE) classified into two types and to further determine if there was any correlation between the shape of endoscopically suspected esophageal metaplasia (ESEM), prevale...AIM:To test this hypothesis of barrett esophagus (BE) classified into two types and to further determine if there was any correlation between the shape of endoscopically suspected esophageal metaplasia (ESEM), prevalence of reflux esophagitis (RE) and heartburn. METHODS: A total of 6504 Japanese who underwent endoscopy for their annual stomach check-up were enrolled in this study. BE was detected without histological confirmation that is ESEM. We originally classified cases of ESEM into 3 types based on its shape: Tongue-like (T type), Dome-like (D type) and Wave-like (W type) ESEM. The respective subjects were prospectively asked to complete questionnaires concerning the symptoms of heartburn, dysphagia, and abdominal pain for a one-month period. RESULTS: ESEM was observed in 10.3% of 6504 subjects (ESEM < 1 cm, 9.4%; 1 cm ≤ ESEM < 3 cm, 1.7%; ESEM ≥ 3 cm, 0.5%). The frequency of ESEM was significantly higher in males compared with female subjects. Statistical analysis showed that the prevalence of heartburn and RE were significantly higher in the T type ESEM than in the W type ESEM (P < 0.05). CONCLUSION: The T type ESEM was strongly associated with reflux symptoms and RE whereas the W type ESEM was not associated with GERD.展开更多
The aim of this study was to evaluate the in vitro response of osteoclast-like cells (RAW 264.7 cells) to a bioactive hydroxyapatite (HAP) surface that was modified using the 30% phosphoric ac-id-etching procedure rep...The aim of this study was to evaluate the in vitro response of osteoclast-like cells (RAW 264.7 cells) to a bioactive hydroxyapatite (HAP) surface that was modified using the 30% phosphoric ac-id-etching procedure reported in our previous paper (2013). The cells on the bioactive HAP surface were multinucleated and were larger than those on the untreated HAP surface. The cell occupancies were greater on the bioactive HAP surface than on the untreated HAP surface at 7, 14, 21, and 28 days of differentiation;in particular, the values at 21 and 28 days were significantly larger (P < 0.05 and P < 0.01, respectively). These findings show that the bioactive HAP surface may enhance the adhesion and differentiation of RAW 264.7 cells as well as osteoblast-like cells, indicating its potential as a superior surface for bone tissue engineering.展开更多
In our previous studies, we developed the normal periodontal ligament index (nPLI) and residual periodontal ligament index (rPLI) to estimate residual periodontal ligament support for individual teeth during treatment...In our previous studies, we developed the normal periodontal ligament index (nPLI) and residual periodontal ligament index (rPLI) to estimate residual periodontal ligament support for individual teeth during treatment planning for partially edentulous patients. To illustrate the applicability of the nPLI and rPLI, an occlusal-supporting ability (OSA) score calculated using these indices for the remaining teeth corresponding to Eichner’s subclasses of partial edentulism was charted by numerically assessing the average occlusal support. This OSA score based on the nPLI and rPLI is proposed as a suitable tool for epidemiologic research on the progression of tooth loss and the survival of prostheses. The Ethics Committee for Epidemiologic Study of Hiroshima University approved the study protocol (No. 331).展开更多
In our previous studies, we developed the normal periodontal ligament index (nPLI) and the residual periodontal ligament index (rPLI), to estimate residual periodontal ligament support for individual teeth during trea...In our previous studies, we developed the normal periodontal ligament index (nPLI) and the residual periodontal ligament index (rPLI), to estimate residual periodontal ligament support for individual teeth during treatment planning for partially edentulous patients. The purpose of the current in vitro study was to analyze tooth mobility resulting from periodontal attachment loss, and to determine the application range of both nPLI and rPLI. The association of horizontal load-displacement and conditions of attachment loss was measured in triplicate for each anatomical tooth model at 10-minute intervals, using a universal tester at a crosshead speed of 0.05 mm/min, and a load of 0.1 N. The conditions of attachment loss were: (I) 0 mm (cementoenamel junction), (II) 2 mm attachment level, and (III) two-thirds, and (IV) one-half lengths of normal attachment. Except for the upper first molar, lower lateral incisor, lower first premolar, and the lower first molar, the displacement of each tooth type was increased significantly relative to Level I (P P < 0.01). The results indicated that nPLI at two-thirds of normal attachment and greater, and rPLI at less than two-thirds of normal attachment should be applied, respectively.展开更多
文摘AIM: To investigate the incidence of reflux esophagitis (RE) and H pylori infection in the diabetic patient. METHODS: The incidence of RE and H pylori infection were investigated in 85 patients with diabetes mellitus and the results were compared with controls. RESULTS: The incidence of RE in diabetic patients was 17.6%. Although this tended to be higher in diabetic patients, there were no statistically significant differences between diabetic patients and controls. The incidence of H pylori infection in diabetic patients was 53.7% but no statistically significant difference was seen between diabetic patients and controls in the incidence of H pylori infection. CONCLUSION: No significant differences could be seen between diabetic patients and controls in the incidence of RE and H pylori infection.
文摘AIM:To study the value of serum biomarker tests to differentiate between patients with healthy or diseased stomach mucosa:i.e.those with Helicobacter pylori(H pylori)gastritis or atrophic gastritis,who have a high risk of gastric cancer or peptic ulcer diseases.METHODS:Among 162 Japanese outpatients,pepsinogen-(Pg-)and(Pg)were measured using a conventional Japanese technique,and the European GastroPanel examination(Pg and Pg,gastrin-17 and H pylori antibodies).Gastroscopy with gastric biopsies was performed to classify the patients into those with healthy stomach mucosa,H pylori non-atrophic gastritis or atrophic gastritis.RESULTS:Pg-and Pg assays with the GastroPanel and the Japanese method showed a highly significant correlation.For methodological reasons,however,serum Pg-,but not Pg,was twice as high with the GastroPanel test as with the Japanese test.The biomarker assays revealed that 5%of subjects had advanced atrophic corpus gastritis which was also verified by endoscopic biopsies.GastroPanel examination revealed an additional seven patients who had either advanced atrophic gastritis limited to the antrum or antrum-predominant H pylori gastritis.When compared to the endoscopic biopsy findings,the GastroPanel examination classified the patients into groups with "healthy" or "diseased" stomach mucosa with 94% accuracy,95% sensitivity and 93% specifi city.CONCLUSION:Serum biomarker tests can be used to differentiate between subjects with healthy and diseased gastric mucosa with high accuracy.
文摘AIM: To compare the antisecretory activity and plasma drug concentrations of a single oral dose of 10 mg lafutidine, a novel H2 receptor antagonist, with those of the proton pump inhibitor lansoprazole (LPZ) 30 mg. METHODS: Ten volunteers without H pylori infection participated in this crossover study comparing lafutidine 10 mg with LPZ 30 mg. Intragastric pH was monitored for 6 h in all participants, and blood samples were collected from four randomly selected individuals after single-dose administration of each drug. RESULTS: The median intragastric pH was significantly higher in individuals who received lafutidine 10 mg than in those who received LPZ 30 mg 2, 3, 4, 5, and 6 h after administration. Maximal plasma drug concentration was reached more promptly with lafutidine 10 mg than with LPZ 30 mg. CONCLUSION: In H pylori-negative individuals, gastric acid secretion is more markedly inhibited by lafutidinethan by LPZ.
文摘AIM:To examine the prevalence of gastroesophageal reflux disease (GERD) symptoms in a large unselected general population in Japan. METHODS: In Japan, mature adults are offered regular check-ups for the prevention of gastric cancer. A notice was sent by mail to all inhabitants aged > 40 years. A total of 160 983 Japanese (60 774 male, 100 209 female; mean age 61.9 years) who underwent a stomach check up were enrolled in this study. In addition, from these 160 983 subjects, we randomly selected a total of 82 894 (34 275 male, 48 619 female; mean age 62.4 years) to evaluate the prevalence of abdominal pain. The respective subjects were prospectively asked to complete questionnaires concerning the symptoms of heartburn, dysphagia, and abdominal pain for a 1 mo period. RESULTS: The respective prevalences of the symptoms in males and females were: heartburn, 15.8% vs 20.7%; dysphagia, 5.4% vs 7.8%; and abdominal pain, 6.6% vs 9.6%. Among these symptoms, heartburn was significantly high compared with the other symptoms, and the prevalence of heartburn was significantly more frequent in females than in males in the 60-89-year agegroup. Dysphagia was also significantly more frequent in female patients. CONCLUSION: The prevalence of typical GERD symptoms (heartburn) was high, at about 20% of the Japan population, and the frequency was especially high in females in the 60-89 year age group.
文摘AIM: To compare rabeprazole (RPZ; 10 mg) with Lansoprazole orally disintegrating tablets (LPZ; 30 mg OD) in terms of antisecretory activity and blood drug concentration after a single dose. METHODS: Eight H pylori-negative cytochrome P450 (CYP) 2C19 extensive metabolizers were assigned to receive a single oral dose of RPZ 10 mg or LPZ 30 mg OD. Twelve hour intragastric pH monitoring was perform- ed on the day of treatment. Blood samples were also collected after the administration of each drug. RESULTS: LPZ 30 mg OD induced a significantly earlier rise in blood drug concentration than RPZ 10 mg; consequently, LPZ 30 mg OD induced a significantly earlier rise in median pH in the third and fourth hours of the study. CONCLUSION: In H pylori-negative CYP2C19 extensive metabolizers, LPZ 30 mg OD induced a significantly faster inhibition of gastric acid secretion than RPZ 10 mg.
文摘AIM:To assess each layer of the optical coherence tomography(OCT)image of the esophageal wall with reference to the histological structure. METHODS:Resected specimens of fresh pig esophagus was used as a model for the esophageal wall.We injected cyanoacrylate adhesive into the specimens to create a marker,and scanned them using a miniature OCT probe.The localization of these markers was assessed in the OCT images.Then we compared the OCT-imaged morphology with the corresponding histological section,guided by the cyanoacrylate adhesive markers.We prepared a second set of experiments using nylon sutures as markers. RESULTS:The OCT image of the esophageal specimen has a clear five-layered morphology. First,it consisted of a relatively less reflective layer; second,a more reflective layer;third,a less reflective layer;fourth,a more reflective layer;and fifth,a less reflective layer.Comparing the OCT images with marked histological sections showed that the first layer corresponded to stratified squamous epithelium; the second to lamina propria;the third to muscularis mucosa;fourth,submucosa;and fifth,muscularispropria with deeper structures of the esophageal wall.CONCLUSION:We demonstrated that the OCT image of the normal esophageal wall showed a five- layered morphology,which corresponds to histological esophageal wall components.
基金Supported by (in part) A JST Grant on Research and Education in Molecular Imaging and Grant-in-Aid for Young Scientists (B), KAKENHI No. 19790465
文摘AIM: To investigate symptoms and brain activity following esophageal acid infusion. METHODS: Fifteen healthy volunteers were recruited for the study. Hydrochloric acid (pH 1 and 2) and distilled water (pH 7) were randomly and repeatedly infused into the esophagus. The brain activity was evaluated by positron emission tomography. The severity of heartburn elicited by the infusion was rated on an auditory analog scale of 0-10.RESULTS: The severity of heartburn following each infusion showed a step-wise increase with increasing acidity of the perfusate. The heartburn scores were significantly higher in the second pH 1 infusion compared with the first infusion. Acid and distilled water infusion induced activation of various brain areas such as the anterior insula, temporal gyrus, and anterior/ posterior cingulate cortex. At pH 1 or 2, in particular, activation was observed in some emotion-related brain areas such as the more anterior part of the anterior cingulate cortex, parahippocampal gyrus, or the temporal pole. Strong activation of the orbitofrontal cortex was found by subtraction analysis of the two second pH 1 infusions, with a significant increase of heartburn symptoms. CONCLUSION: Emotion-related brain areas were activated by esophageal acid stimulation. The orbitofrontal area might be involved in symptom processing, with esophageal sensitization induced by repeated acid stimulation.
文摘AIM:To test this hypothesis of barrett esophagus (BE) classified into two types and to further determine if there was any correlation between the shape of endoscopically suspected esophageal metaplasia (ESEM), prevalence of reflux esophagitis (RE) and heartburn. METHODS: A total of 6504 Japanese who underwent endoscopy for their annual stomach check-up were enrolled in this study. BE was detected without histological confirmation that is ESEM. We originally classified cases of ESEM into 3 types based on its shape: Tongue-like (T type), Dome-like (D type) and Wave-like (W type) ESEM. The respective subjects were prospectively asked to complete questionnaires concerning the symptoms of heartburn, dysphagia, and abdominal pain for a one-month period. RESULTS: ESEM was observed in 10.3% of 6504 subjects (ESEM < 1 cm, 9.4%; 1 cm ≤ ESEM < 3 cm, 1.7%; ESEM ≥ 3 cm, 0.5%). The frequency of ESEM was significantly higher in males compared with female subjects. Statistical analysis showed that the prevalence of heartburn and RE were significantly higher in the T type ESEM than in the W type ESEM (P < 0.05). CONCLUSION: The T type ESEM was strongly associated with reflux symptoms and RE whereas the W type ESEM was not associated with GERD.
文摘The aim of this study was to evaluate the in vitro response of osteoclast-like cells (RAW 264.7 cells) to a bioactive hydroxyapatite (HAP) surface that was modified using the 30% phosphoric ac-id-etching procedure reported in our previous paper (2013). The cells on the bioactive HAP surface were multinucleated and were larger than those on the untreated HAP surface. The cell occupancies were greater on the bioactive HAP surface than on the untreated HAP surface at 7, 14, 21, and 28 days of differentiation;in particular, the values at 21 and 28 days were significantly larger (P < 0.05 and P < 0.01, respectively). These findings show that the bioactive HAP surface may enhance the adhesion and differentiation of RAW 264.7 cells as well as osteoblast-like cells, indicating its potential as a superior surface for bone tissue engineering.
文摘In our previous studies, we developed the normal periodontal ligament index (nPLI) and residual periodontal ligament index (rPLI) to estimate residual periodontal ligament support for individual teeth during treatment planning for partially edentulous patients. To illustrate the applicability of the nPLI and rPLI, an occlusal-supporting ability (OSA) score calculated using these indices for the remaining teeth corresponding to Eichner’s subclasses of partial edentulism was charted by numerically assessing the average occlusal support. This OSA score based on the nPLI and rPLI is proposed as a suitable tool for epidemiologic research on the progression of tooth loss and the survival of prostheses. The Ethics Committee for Epidemiologic Study of Hiroshima University approved the study protocol (No. 331).
文摘In our previous studies, we developed the normal periodontal ligament index (nPLI) and the residual periodontal ligament index (rPLI), to estimate residual periodontal ligament support for individual teeth during treatment planning for partially edentulous patients. The purpose of the current in vitro study was to analyze tooth mobility resulting from periodontal attachment loss, and to determine the application range of both nPLI and rPLI. The association of horizontal load-displacement and conditions of attachment loss was measured in triplicate for each anatomical tooth model at 10-minute intervals, using a universal tester at a crosshead speed of 0.05 mm/min, and a load of 0.1 N. The conditions of attachment loss were: (I) 0 mm (cementoenamel junction), (II) 2 mm attachment level, and (III) two-thirds, and (IV) one-half lengths of normal attachment. Except for the upper first molar, lower lateral incisor, lower first premolar, and the lower first molar, the displacement of each tooth type was increased significantly relative to Level I (P P < 0.01). The results indicated that nPLI at two-thirds of normal attachment and greater, and rPLI at less than two-thirds of normal attachment should be applied, respectively.
