Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breedin...Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG.展开更多
目的:观察两种平衡训练策略对脑卒中患者下肢运动功能恢复的影响。方法:选取脑卒中患者63例,采用随机数字表法将其随机分为对照组和观察组,其中对照组31例,观察组32例。对照组在常规康复治疗的基础上,接受姿势矫正镜下的平衡训练;观察...目的:观察两种平衡训练策略对脑卒中患者下肢运动功能恢复的影响。方法:选取脑卒中患者63例,采用随机数字表法将其随机分为对照组和观察组,其中对照组31例,观察组32例。对照组在常规康复治疗的基础上,接受姿势矫正镜下的平衡训练;观察组患者在常规康复治疗的基础上,接受平衡训练仪下的平衡训练。两组患者的平衡训练时间均为4周。于治疗前和治疗4周后采用部分静态平衡仪测试参数(轨迹总长度、轨迹面积图和速度图)、Berg平衡量表(Berg Balance Scale,BBS)、10 m最大步行速度(Maximum Walking Speed,MWS)和计时起立-行走测试(Timed Up and Go Test,TUGT),分别评估患者的静态平衡能力、步行速度和移动能力。结果:治疗4周后,行组内比较,两组患者的部分静态平衡仪测试参数、BBS、MWS和TUGT结果较治疗前均显著改善,差异有统计学意义(P<0.05);组间比较,两组患者的部分静态平衡仪测试参数、BBS和MWS相比,差异有统计学意义(P<0.05);而两组的TUGT结果相比,差别无统计学意义(P>0.05)。结论:与姿势矫正镜下进行平衡训练相比,采用平衡训练仪能够有效改善脑卒中患者的静态平衡下压力中心(Center of Pressure,COP)的轨迹总长度、轨迹面积图和速度图,能提高患者的平衡能力和步行速度,加速患者下肢运动功能的恢复。展开更多
The layered pavements usually exhibit complicated mechanical properties with the effect of complex material properties under external environment.In some cases,such as launching missiles or rockets,layered pavements a...The layered pavements usually exhibit complicated mechanical properties with the effect of complex material properties under external environment.In some cases,such as launching missiles or rockets,layered pavements are required to bear large impulse load.However,traditional methods cannot non-destructively and quickly detect the internal structural of pavements.Thus,accurate and fast prediction of the mechanical properties of layered pavements is of great importance and necessity.In recent years,machine learning has shown great superiority in solving nonlinear problems.In this work,we present a method of predicting the maximum deflection and damage factor of layered pavements under instantaneous large impact based on random forest regression with the deflection basin parameters obtained from falling weight deflection testing.The regression coefficient R^(2)of testing datasets are above 0.94 in the process of predicting the elastic moduli of structural layers and mechanical responses,which indicates that the prediction results have great consistency with finite element simulation results.This paper provides a novel method for fast and accurate prediction of pavement mechanical responses under instantaneous large impact load using partial structural parameters of pavements,and has application potential in non-destructive evaluation of pavement structure.展开更多
The dynamic traveling salesman problem(DTSP)is significant in logistics distribution in real-world applications in smart cities,but it is uncertain and difficult to solve.This paper proposes a scheme library-based ant...The dynamic traveling salesman problem(DTSP)is significant in logistics distribution in real-world applications in smart cities,but it is uncertain and difficult to solve.This paper proposes a scheme library-based ant colony optimization(ACO)with a two-optimization(2-opt)strategy to solve the DTSP efficiently.The work is novel and contributes to three aspects:problemmodel,optimization framework,and algorithmdesign.Firstly,in the problem model,traditional DTSP models often consider the change of travel distance between two nodes over time,while this paper focuses on a special DTSP model in that the node locations change dynamically over time.Secondly,in the optimization framework,the ACO algorithm is carried out in an offline optimization and online application framework to efficiently reuse the historical information to help fast respond to the dynamic environment.The framework of offline optimization and online application is proposed due to the fact that the environmental change inDTSPis caused by the change of node location,and therefore the newenvironment is somehowsimilar to certain previous environments.This way,in the offline optimization,the solutions for possible environmental changes are optimized in advance,and are stored in a mode scheme library.In the online application,when an environmental change is detected,the candidate solutions stored in the mode scheme library are reused via ACO to improve search efficiency and reduce computational complexity.Thirdly,in the algorithm design,the ACO cooperates with the 2-opt strategy to enhance search efficiency.To evaluate the performance of ACO with 2-opt,we design two challenging DTSP cases with up to 200 and 1379 nodes and compare them with other ACO and genetic algorithms.The experimental results show that ACO with 2-opt can solve the DTSPs effectively.展开更多
Objective:Gestational diabetes mellitus(GDM)is the most common metabolic disorder during pregnancy.LncRNA HLA complex group 27(HCG27)plays a crucial role in various metabolic diseases.However,the relationship between ...Objective:Gestational diabetes mellitus(GDM)is the most common metabolic disorder during pregnancy.LncRNA HLA complex group 27(HCG27)plays a crucial role in various metabolic diseases.However,the relationship between lncRNA HCG27 and GDM is not clear.This study aimed to verify a competing endogenous RNA(ceRNA)interaction regulation axis of miR-378a-3p/mitogen-activated protein kinase 1(MAPK1)regulated by HCG27 in GDM.Methods:LncRNA HCG27 and miR-378a-3p were detected by RT-qPCR.The expression of MAPK1 in umbilical vein endothelial cells(HUVECs)was detected by RT-qPCR and that in the placenta by Western blotting.To explore the relationship among lncRNA HCG27,miR-378a-3p,MAPK1 and the glucose uptake ability of HUVECs,vector HCG27,si-HCG27,miR-378a-3p mimic and inhibitor were transfected to achieve overexpression and inhibition of HCG27 or miR-378a-3p.The interaction between miR-378a-3p and lncRNA HCG27 or MAPK1 was confirmed by the dual-luciferase reporter assay.Besides,glucose consumption by HUVECs was detected by the glucose assay kit.Results:HCG27 expression was significantly decreased in both the placenta and primary umbilical vein endothelial cells,while the expression of miR-378a-3p was significantly increased in GDM tissues,and the expression of MAPK1 was decreased in GDM tissues.This ceRNA interaction regulation axiswas proved to affect the glucose uptake function of HUVECs.The transfection of si-HCG27 could significantly reduce the expression of the MAPK1 protein.If the MAPK1 overexpression plasmid was transfected simultaneously with si-HCG27 transfection,the reduced glucose uptake in HUVECs resulting from the decrease in lncRNA HCG27 was reversed.MiR-378a-3p mimic can significantly reduce the mRNA expression of MAPK1 in HUVECs,whereas miR-378a-3p inhibitor can significantly increase the mRNA expression of MAPK1.The inhibition of miR-378a-3p could restore the decreased glucose uptake of HUVECs treated with si-HCG27.Besides,overexpression of lncRNA HCG27 could restore the glucose uptake ability of the palmitic acid-induced insulin resistance model of HUVECs to normal.Conclusion:LncRNA HCG27 promotes glucose uptake of HUVECs by miR-378a-3p/MAPK1 pathway,which may provide potential therapeutic targets for GDM.Besides,the fetal umbilical cord blood and umbilical vein endothelial cells collected from pregnant women with GDM after delivery could be used to detect the presence of adverse molecular markers of metabolic memory,so as to provide guidance for predicting the risk of cardiovascular diseases and health screening of offspring.展开更多
The Earth’s“lithosphere”is its outer shell,made up of the Earth’s crust and outermost mantle.The part of the Earth’s magnetic field that originates in the lithosphere consists of a superposition of magnetic anoma...The Earth’s“lithosphere”is its outer shell,made up of the Earth’s crust and outermost mantle.The part of the Earth’s magnetic field that originates in the lithosphere consists of a superposition of magnetic anomalies with a broad spectrum of sizes and intensities,which arise from geological and tectonic features.The lithospheric magnetic field is known from surface observations,and on larger scales from above-surface measurements.The increase in recent decades of satellites dedicated to measuring the Earth’s magnetic field has improved significantly our models of the Earth’s magnetic environment.Based on these increasing observations,a number of comprehensive field models have been constructed,some of which focus solely on the lithosphere,such as the MF model series.We present a map of lithospheric magnetic anomalies at 400 km altitude,based on a vertically integrated magnetization model.This height was chosen because it is the expected orbital altitude of the Macao Science Satellite-1(MSS-1)mission.The model presented herein indicates that the amplitude of the lithospheric anomalies at 400 km altitude is between-14.8 n T and 18.2 n T.This information is useful because it provides a reference for the lithospheric source of the Earth’s magnetic field that contributes to the magnetic measurements made from satellite instruments.The low inclination orbit of the MSS-1 mission will provide information that is sensitive to lateral variation within the lithosphere;these variations arise from plate tectonic features with longitudinal extent.In conclusion,the new MSS-1mission will provide valuable information in detecting compositional variations in the lithosphere,and in delineating large-scale geological structures.展开更多
A future constellation of at least four geomagnetic satellites(designated Macao Scientific Satellite-1(MSS-1)and Macao Scientific Satellite-2(MSS-2))was recently proposed,to continue high-quality geomagnetic observati...A future constellation of at least four geomagnetic satellites(designated Macao Scientific Satellite-1(MSS-1)and Macao Scientific Satellite-2(MSS-2))was recently proposed,to continue high-quality geomagnetic observations in the post-Swarm period,focusing especially on collecting data that will provide a global,three-dimensional survey of the geomagnetic field.In this paper,we present a simulation of two years of orbits(2020.01.01-2022.01.01)of two satellites(tentatively denoted as MSS-2)that are constellated in elliptical(200×5,300 km)low-perigee orbits.By comparing error variances of Gauss coefficients,we investigate the sensitivity of lithospheric magnetic field modelling to data collected from various satellite orbits,including a near circular reference orbit of 300×350km,and elliptical orbit of 180×5,300 km,220×5,300 km,200×3,000 km and 200×1,500 km.We find that in two years the two MSS-2 satellites can collect 35,000 observations at altitude below 250 km,data that will be useful in advancing the quality of lithospheric magnetic field modelling;this number of observations reflects the fact that only 4.5%of the flight time of these satellites will be below250 km(just 6.4%of their flight time below 300 km).By combining observations from the MSS-2 satellites’elliptical orbits of 200×5,300km with observations from a circular reference orbit,the variance of the geomagnetic model can be reduced by a factor of 285 at spherical harmonic degree n=200 and by a factor of 1,300 at n=250.The planned lower perigee of their orbits allows the new satellites to collect data at unprecedentedly lower altitudes,thus dramatically improving the spatial resolution of satellite-derived lithospheric field models,(up to 80%at n=150).In addition,lowering the apogee increases the time interval during which the satellites fly at near-Earth altitudes,thus improving the model predictions at all spherical harmonic degrees(around 52%-62%at n=150).The upper limit of the expected improvement to the field model at the orbital apogee is not as good as at the perigee.However,data from the MSS-1 orbit can help fill the gap between data from the MSS-2 orbits and from the circular reference orbit for the low-degree part of the model.The feasibility of even lower-altitude flight requires further discussion with satellite engineers.展开更多
The Euler angle estimation is a calibration method for vector data measured by the magnetometer on a satellite.It is used to find the relative rotation between the coordinate system of the magnetometer and the satelli...The Euler angle estimation is a calibration method for vector data measured by the magnetometer on a satellite.It is used to find the relative rotation between the coordinate system of the magnetometer and the satellite(usually determined by Star Imagers).Before launch of the low-orbit,low-inclination Macao Science Satellite-1(known as MSS-1),we simulated the estimation of Euler angles by using the magnetic measurements of the in-orbit Swarm and China Seismo-Electromagnetic Satellite(noted as CSES),with various data combinations.In this study,11 data sets were designed to analyze the estimation results for the MSS-1 orbit by using a joint estimation method of the geomagnetic field model parameters and Euler angles.For the model results,we found that all the spatial power spectral lines showed behavior consistent with that of the CHAOS-7.8 model at low degrees(corresponding to large-scale magnetic signals).The spectra of models without global data coverage deviated much more(by a maximum of~10^(4) nT^(2))from those of the CHAOS-7.8 model at higher degrees.For models with global data coverage and with various data combinations,the spectral lines were distributed similarly.Moreover,the models with accordant power spectral distributions demonstrated different Euler angle estimations.As more vector data at higher latitudes were included,the estimated Euler angles varied monotonically in all three directions.The models with vector data in the same latitude range showed similar Euler angle results,regardless of whether the poleward scalar data were included.The largest value difference was found between the models using vector data within±40°latitudes and those using vector data within±60°latitudes,which reached to~28″.Therefore,we concluded that the inversion of the spherical harmonic Gauss coefficients in our tests was mainly affected by the spatial coverage range of the data,whereas the estimation of Euler angles largely depended on the latitude range where the vector data could be obtained.These results can be used for future in-flight data testing.We expect the estimation of Euler angles to improve as other methods are adopted.展开更多
The k·p method is significant in condensed matter physics for the compact and analytical Hamiltonian.In the presence of magnetic field,it is described by the effective Zeeman's coupling Hamiltonian with Land&...The k·p method is significant in condensed matter physics for the compact and analytical Hamiltonian.In the presence of magnetic field,it is described by the effective Zeeman's coupling Hamiltonian with Landég-factors.Here,we develop an open-source package VASP2KP(including two parts:vasp2mat and mat2kp)to compute k·p parameters and Landég-factors directly from the wavefunctions provided by the density functional theory(DFT)as implemented in Vienna ab initio Simulation Package(VASP).First,we develop a VASP patch vasp2mat to compute matrix representations of the generalized momentum operatorπ=p+1/2mc^(2)[s×■V(r)],spin operator s,time reversal operatorT,and crystalline symmetry operatorsR on the DFT wavefunctions.Second,we develop a python code mat2kp to obtain the unitary transformation U that rotates the degenerate DFT basis towards the standard basis,and then automatically compute the k·p parameters and g-factors.The theory and the methodology behind VASP2KP are described in detail.The matrix elements of the operators are derived comprehensively and computed correctly within the projector augmented wave method.We apply this package to some materials,e.g.,Bi2Se3,Na3Bi,Te,InAs and 1H-TMD monolayers.The obtained effective model's dispersions are in good agreement with the DFT data around the specific wave vector,and the g-factors are consistent with experimental data.The VASP2KP package is available at https://github.com/zjwang11/VASP2KP.展开更多
In view of the effective perception demand of PD(Partial Discharge)insulation defect of in-service GIS(Gas Insulated Switchgear),the external UHF(Ultra High Frequency,0.3−3 GHz)sensing technology for GIS PD insulation...In view of the effective perception demand of PD(Partial Discharge)insulation defect of in-service GIS(Gas Insulated Switchgear),the external UHF(Ultra High Frequency,0.3−3 GHz)sensing technology for GIS PD insulation defect fromresin hole is studied in this paper.Firstly,rectangular waveguide theory is used to analyze the electromagnetic wave propagation characteristics from rectangular resin hole in GIS disc insulator.It is found that the energy of leaked electromagnetic wave mainly concentrates in the frequency band above 1 GHz.Then based on this frequency band characteristics,a cavity-backed bowtie sensor for GIS PD external detection is designed optimized by HFSS finite element software,in band of 1–3 GHz,its maximum VSWR is 7,the minimum is 1.1,the average is 2.9,and in band of 0.3–1 GHz,the maximum and minimum VSWR is 42.8 and 7,the average is 20.6.Finally,a sensor prototype is made,its sensitivity experiment is carried out on 220 kV GIS,experimental results show that the designed sensor can stably detect the PD electromagnetic wave signal leaked fromthe resin hole,and the signal energy in frequency band range is consistent with the theoretical analysis results.展开更多
The American chestnut (<em>Castanea dentata</em>) was once a dominant tree species in the Appalachian Mountains and played a critical role in the ecological system. However, it was nearly eliminated by che...The American chestnut (<em>Castanea dentata</em>) was once a dominant tree species in the Appalachian Mountains and played a critical role in the ecological system. However, it was nearly eliminated by chestnut blight caused by the Ascomycetous fungus <em>Cryphonectria parasitica</em>. Identification of compounds specific to species and backcross hybrids may help further refine disease resistance breeding and testing. Phenolic compounds produced by plants are significant to their defense mechanisms against fungal pathogens. Therefore, an analytical platform has been developed to estimate the total phenolic content in leaf tissues of the American chestnut, Chinese chestnut (<em>Castanea mollissima</em>), and their backcross breeding generations (B<sub>3</sub>F<sub>2</sub> and B<sub>3</sub>F<sub>3</sub>) using the Folin-Ciocalteu reagent assay with UV/Vis spectrophotometry which may be used to predict blight resistance. Adsorption (765 nm) results from leaf tissue extraction in methanol/water (95%:5% v/v) and pH 2, show that the variations among these four tree species are significant (ANOVA p = 2.3 × 10<sup>-7</sup>). The kinetics of phenolic compound solid-liquid extraction was elaborated using Peleg, second order, Elovich, and power law models. In addition, extensive analysis using headspace solid phase microextraction (SPME) gas chromatography and mass spectrometry was conducted to identify volatile organic compounds (VOCs) from the leaf of American chestnut, Chinese chestnut, and their backcross hybrids B<sub>3</sub>F<sub>2</sub> and B<sub>3</sub>F<sub>3</sub>. A total of 67 VOCs were identified among all chestnut types. Many of the metabolites associated with the Chinese chestnut have been reported to have antifungal properties, whereas the native and hybrid American chestnut metabolites have not. Most of the antifungal metabolites showed the strongest efficacy towards the Ascomycota phylum. A partial least squares discriminant analysis (PLS-DA) model (R<sup>2</sup>X = 0.884, R<sup>2</sup>Y = 0.917, Q<sup>2</sup> = 0.584) differentiated chestnut species and hybrids within the first five principal component (PCs).展开更多
目的:对比分析双眼三焦点IOL(AT LISA tri 839MP IOL)植入与一眼植入AT LISA tri 839MP IOL而另一眼植入非球面单焦点IOL患者术后视觉相关生活质量。方法:前瞻性临床研究。收集2018-05/2019-08在我院行白内障超声乳化联合三焦点AT LISA ...目的:对比分析双眼三焦点IOL(AT LISA tri 839MP IOL)植入与一眼植入AT LISA tri 839MP IOL而另一眼植入非球面单焦点IOL患者术后视觉相关生活质量。方法:前瞻性临床研究。收集2018-05/2019-08在我院行白内障超声乳化联合三焦点AT LISA tri 839MP IOL植入的患者45例,按是否双眼植入AT LISA tri 839MP IOL分为两组,双眼植入者24例纳入双眼三焦点组;一眼植入AT LISA tri 839MP IOL而另一眼植入非球面单焦点IOL者21例纳入单眼三焦点组。术后3mo,检测两组患者裸眼视力及等效球镜度,并通过中国版视功能指数量表(VF-14-CN)评价视觉相关生活质量,评估日常生活脱镜率及满意度。结果:术后3mo,双眼三焦点组和单眼三焦点组患者双眼裸眼远、中、近视力均无明显差异(P>0.05),两组患者VF-14-CN问卷得分(96.2±0.50、92.43±1.32分)、日常生活脱镜率(96%、90%)及患者主观满意度(96%、95%)均无差异(P>0.05)。结论:双眼植入AT LISA tri 839MP IOL和一眼植入AT LISA tri 839MP IOL而另一眼植入非球面单焦点IOL的患者术后均具有良好的双眼裸眼远、中、近视力,术后视觉相关生活质量、脱镜率、满意度无明显差异。对一眼不适合植入三焦点IOL或既往一眼已植入非球面单焦点IOL的患者,可考虑另一眼行三焦点IOL植入以满足患者双眼全程视力的要求。展开更多
基金This work was supported by the National Natural Science Foundation of China(32071943,32272198).
文摘Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG.
文摘目的:观察两种平衡训练策略对脑卒中患者下肢运动功能恢复的影响。方法:选取脑卒中患者63例,采用随机数字表法将其随机分为对照组和观察组,其中对照组31例,观察组32例。对照组在常规康复治疗的基础上,接受姿势矫正镜下的平衡训练;观察组患者在常规康复治疗的基础上,接受平衡训练仪下的平衡训练。两组患者的平衡训练时间均为4周。于治疗前和治疗4周后采用部分静态平衡仪测试参数(轨迹总长度、轨迹面积图和速度图)、Berg平衡量表(Berg Balance Scale,BBS)、10 m最大步行速度(Maximum Walking Speed,MWS)和计时起立-行走测试(Timed Up and Go Test,TUGT),分别评估患者的静态平衡能力、步行速度和移动能力。结果:治疗4周后,行组内比较,两组患者的部分静态平衡仪测试参数、BBS、MWS和TUGT结果较治疗前均显著改善,差异有统计学意义(P<0.05);组间比较,两组患者的部分静态平衡仪测试参数、BBS和MWS相比,差异有统计学意义(P<0.05);而两组的TUGT结果相比,差别无统计学意义(P>0.05)。结论:与姿势矫正镜下进行平衡训练相比,采用平衡训练仪能够有效改善脑卒中患者的静态平衡下压力中心(Center of Pressure,COP)的轨迹总长度、轨迹面积图和速度图,能提高患者的平衡能力和步行速度,加速患者下肢运动功能的恢复。
基金Project supported in part by the National Natural Science Foundation of China(Grant No.12075168)the Fund from the Science and Technology Commission of Shanghai Municipality(Grant No.21JC1405600)。
文摘The layered pavements usually exhibit complicated mechanical properties with the effect of complex material properties under external environment.In some cases,such as launching missiles or rockets,layered pavements are required to bear large impulse load.However,traditional methods cannot non-destructively and quickly detect the internal structural of pavements.Thus,accurate and fast prediction of the mechanical properties of layered pavements is of great importance and necessity.In recent years,machine learning has shown great superiority in solving nonlinear problems.In this work,we present a method of predicting the maximum deflection and damage factor of layered pavements under instantaneous large impact based on random forest regression with the deflection basin parameters obtained from falling weight deflection testing.The regression coefficient R^(2)of testing datasets are above 0.94 in the process of predicting the elastic moduli of structural layers and mechanical responses,which indicates that the prediction results have great consistency with finite element simulation results.This paper provides a novel method for fast and accurate prediction of pavement mechanical responses under instantaneous large impact load using partial structural parameters of pavements,and has application potential in non-destructive evaluation of pavement structure.
基金supported in part by the National Research Foundation of Korea (NRF-2021H1D3A2A01082705).
文摘The dynamic traveling salesman problem(DTSP)is significant in logistics distribution in real-world applications in smart cities,but it is uncertain and difficult to solve.This paper proposes a scheme library-based ant colony optimization(ACO)with a two-optimization(2-opt)strategy to solve the DTSP efficiently.The work is novel and contributes to three aspects:problemmodel,optimization framework,and algorithmdesign.Firstly,in the problem model,traditional DTSP models often consider the change of travel distance between two nodes over time,while this paper focuses on a special DTSP model in that the node locations change dynamically over time.Secondly,in the optimization framework,the ACO algorithm is carried out in an offline optimization and online application framework to efficiently reuse the historical information to help fast respond to the dynamic environment.The framework of offline optimization and online application is proposed due to the fact that the environmental change inDTSPis caused by the change of node location,and therefore the newenvironment is somehowsimilar to certain previous environments.This way,in the offline optimization,the solutions for possible environmental changes are optimized in advance,and are stored in a mode scheme library.In the online application,when an environmental change is detected,the candidate solutions stored in the mode scheme library are reused via ACO to improve search efficiency and reduce computational complexity.Thirdly,in the algorithm design,the ACO cooperates with the 2-opt strategy to enhance search efficiency.To evaluate the performance of ACO with 2-opt,we design two challenging DTSP cases with up to 200 and 1379 nodes and compare them with other ACO and genetic algorithms.The experimental results show that ACO with 2-opt can solve the DTSPs effectively.
基金supported by the National Key Research and Development Program of China(No.2021YFC2701502)the Health Commission of Scientific Research Project of Hubei Province(No.WJ2021M129).
文摘Objective:Gestational diabetes mellitus(GDM)is the most common metabolic disorder during pregnancy.LncRNA HLA complex group 27(HCG27)plays a crucial role in various metabolic diseases.However,the relationship between lncRNA HCG27 and GDM is not clear.This study aimed to verify a competing endogenous RNA(ceRNA)interaction regulation axis of miR-378a-3p/mitogen-activated protein kinase 1(MAPK1)regulated by HCG27 in GDM.Methods:LncRNA HCG27 and miR-378a-3p were detected by RT-qPCR.The expression of MAPK1 in umbilical vein endothelial cells(HUVECs)was detected by RT-qPCR and that in the placenta by Western blotting.To explore the relationship among lncRNA HCG27,miR-378a-3p,MAPK1 and the glucose uptake ability of HUVECs,vector HCG27,si-HCG27,miR-378a-3p mimic and inhibitor were transfected to achieve overexpression and inhibition of HCG27 or miR-378a-3p.The interaction between miR-378a-3p and lncRNA HCG27 or MAPK1 was confirmed by the dual-luciferase reporter assay.Besides,glucose consumption by HUVECs was detected by the glucose assay kit.Results:HCG27 expression was significantly decreased in both the placenta and primary umbilical vein endothelial cells,while the expression of miR-378a-3p was significantly increased in GDM tissues,and the expression of MAPK1 was decreased in GDM tissues.This ceRNA interaction regulation axiswas proved to affect the glucose uptake function of HUVECs.The transfection of si-HCG27 could significantly reduce the expression of the MAPK1 protein.If the MAPK1 overexpression plasmid was transfected simultaneously with si-HCG27 transfection,the reduced glucose uptake in HUVECs resulting from the decrease in lncRNA HCG27 was reversed.MiR-378a-3p mimic can significantly reduce the mRNA expression of MAPK1 in HUVECs,whereas miR-378a-3p inhibitor can significantly increase the mRNA expression of MAPK1.The inhibition of miR-378a-3p could restore the decreased glucose uptake of HUVECs treated with si-HCG27.Besides,overexpression of lncRNA HCG27 could restore the glucose uptake ability of the palmitic acid-induced insulin resistance model of HUVECs to normal.Conclusion:LncRNA HCG27 promotes glucose uptake of HUVECs by miR-378a-3p/MAPK1 pathway,which may provide potential therapeutic targets for GDM.Besides,the fetal umbilical cord blood and umbilical vein endothelial cells collected from pregnant women with GDM after delivery could be used to detect the presence of adverse molecular markers of metabolic memory,so as to provide guidance for predicting the risk of cardiovascular diseases and health screening of offspring.
基金supported by the Macao Foundationby the Preresearch Project on Civil Aerospace Technologies No.D020308/D020303 funded by China National Space Administration+1 种基金by the Macao Science and Technology Development Fund,grant No.0001/2019/A1PF Liu is funded by the Science and Technology Development Fund,Macao SAR(File No.0002/2019/APD)。
文摘The Earth’s“lithosphere”is its outer shell,made up of the Earth’s crust and outermost mantle.The part of the Earth’s magnetic field that originates in the lithosphere consists of a superposition of magnetic anomalies with a broad spectrum of sizes and intensities,which arise from geological and tectonic features.The lithospheric magnetic field is known from surface observations,and on larger scales from above-surface measurements.The increase in recent decades of satellites dedicated to measuring the Earth’s magnetic field has improved significantly our models of the Earth’s magnetic environment.Based on these increasing observations,a number of comprehensive field models have been constructed,some of which focus solely on the lithosphere,such as the MF model series.We present a map of lithospheric magnetic anomalies at 400 km altitude,based on a vertically integrated magnetization model.This height was chosen because it is the expected orbital altitude of the Macao Science Satellite-1(MSS-1)mission.The model presented herein indicates that the amplitude of the lithospheric anomalies at 400 km altitude is between-14.8 n T and 18.2 n T.This information is useful because it provides a reference for the lithospheric source of the Earth’s magnetic field that contributes to the magnetic measurements made from satellite instruments.The low inclination orbit of the MSS-1 mission will provide information that is sensitive to lateral variation within the lithosphere;these variations arise from plate tectonic features with longitudinal extent.In conclusion,the new MSS-1mission will provide valuable information in detecting compositional variations in the lithosphere,and in delineating large-scale geological structures.
基金Y Jiang is supported by the Macao FoundationMacao Science and Technology Development Fund,grant No.0001/2019/A1by the Pre-research Project on Civil Aerospace Technologies No.D020303/D020308 funded by China National Space Administration。
文摘A future constellation of at least four geomagnetic satellites(designated Macao Scientific Satellite-1(MSS-1)and Macao Scientific Satellite-2(MSS-2))was recently proposed,to continue high-quality geomagnetic observations in the post-Swarm period,focusing especially on collecting data that will provide a global,three-dimensional survey of the geomagnetic field.In this paper,we present a simulation of two years of orbits(2020.01.01-2022.01.01)of two satellites(tentatively denoted as MSS-2)that are constellated in elliptical(200×5,300 km)low-perigee orbits.By comparing error variances of Gauss coefficients,we investigate the sensitivity of lithospheric magnetic field modelling to data collected from various satellite orbits,including a near circular reference orbit of 300×350km,and elliptical orbit of 180×5,300 km,220×5,300 km,200×3,000 km and 200×1,500 km.We find that in two years the two MSS-2 satellites can collect 35,000 observations at altitude below 250 km,data that will be useful in advancing the quality of lithospheric magnetic field modelling;this number of observations reflects the fact that only 4.5%of the flight time of these satellites will be below250 km(just 6.4%of their flight time below 300 km).By combining observations from the MSS-2 satellites’elliptical orbits of 200×5,300km with observations from a circular reference orbit,the variance of the geomagnetic model can be reduced by a factor of 285 at spherical harmonic degree n=200 and by a factor of 1,300 at n=250.The planned lower perigee of their orbits allows the new satellites to collect data at unprecedentedly lower altitudes,thus dramatically improving the spatial resolution of satellite-derived lithospheric field models,(up to 80%at n=150).In addition,lowering the apogee increases the time interval during which the satellites fly at near-Earth altitudes,thus improving the model predictions at all spherical harmonic degrees(around 52%-62%at n=150).The upper limit of the expected improvement to the field model at the orbital apogee is not as good as at the perigee.However,data from the MSS-1 orbit can help fill the gap between data from the MSS-2 orbits and from the circular reference orbit for the low-degree part of the model.The feasibility of even lower-altitude flight requires further discussion with satellite engineers.
基金funded by the Macao Foundation,the pre-research project of Civil Aerospace Technologies(Nos.D020308 and D020303)funded by the China National Space Administration,Macao Science and Technology Development Fund(FDCT+1 种基金No.0001/2019/A1)the opening fund of the State Key Laboratory of Lunar and Planetary Sciences(Macao University of Science and Technology,Macao FDCT No.119/2017/A3)。
文摘The Euler angle estimation is a calibration method for vector data measured by the magnetometer on a satellite.It is used to find the relative rotation between the coordinate system of the magnetometer and the satellite(usually determined by Star Imagers).Before launch of the low-orbit,low-inclination Macao Science Satellite-1(known as MSS-1),we simulated the estimation of Euler angles by using the magnetic measurements of the in-orbit Swarm and China Seismo-Electromagnetic Satellite(noted as CSES),with various data combinations.In this study,11 data sets were designed to analyze the estimation results for the MSS-1 orbit by using a joint estimation method of the geomagnetic field model parameters and Euler angles.For the model results,we found that all the spatial power spectral lines showed behavior consistent with that of the CHAOS-7.8 model at low degrees(corresponding to large-scale magnetic signals).The spectra of models without global data coverage deviated much more(by a maximum of~10^(4) nT^(2))from those of the CHAOS-7.8 model at higher degrees.For models with global data coverage and with various data combinations,the spectral lines were distributed similarly.Moreover,the models with accordant power spectral distributions demonstrated different Euler angle estimations.As more vector data at higher latitudes were included,the estimated Euler angles varied monotonically in all three directions.The models with vector data in the same latitude range showed similar Euler angle results,regardless of whether the poleward scalar data were included.The largest value difference was found between the models using vector data within±40°latitudes and those using vector data within±60°latitudes,which reached to~28″.Therefore,we concluded that the inversion of the spherical harmonic Gauss coefficients in our tests was mainly affected by the spatial coverage range of the data,whereas the estimation of Euler angles largely depended on the latitude range where the vector data could be obtained.These results can be used for future in-flight data testing.We expect the estimation of Euler angles to improve as other methods are adopted.
基金supported by the National Key R&D Program of Chain(Grant No.2022YFA1403800)the National Natural Science Foundation of China(Grant Nos.11974395,12188101,11925408,12274436,and 11921004)+5 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDB33000000)the Center for Materials Genomesupported by the Innovation Program for Quantum Science and Technology(Grant No.2021ZD0302403)the National Natural Science Foundation of China(Grant No.12274005)the National Key Research and Development Program of China(Grant No.2021YFA1401900)supported by the Informatization Plan of the Chinese Academy of Sciences(Grant No.CASWX2021SF0102)。
文摘The k·p method is significant in condensed matter physics for the compact and analytical Hamiltonian.In the presence of magnetic field,it is described by the effective Zeeman's coupling Hamiltonian with Landég-factors.Here,we develop an open-source package VASP2KP(including two parts:vasp2mat and mat2kp)to compute k·p parameters and Landég-factors directly from the wavefunctions provided by the density functional theory(DFT)as implemented in Vienna ab initio Simulation Package(VASP).First,we develop a VASP patch vasp2mat to compute matrix representations of the generalized momentum operatorπ=p+1/2mc^(2)[s×■V(r)],spin operator s,time reversal operatorT,and crystalline symmetry operatorsR on the DFT wavefunctions.Second,we develop a python code mat2kp to obtain the unitary transformation U that rotates the degenerate DFT basis towards the standard basis,and then automatically compute the k·p parameters and g-factors.The theory and the methodology behind VASP2KP are described in detail.The matrix elements of the operators are derived comprehensively and computed correctly within the projector augmented wave method.We apply this package to some materials,e.g.,Bi2Se3,Na3Bi,Te,InAs and 1H-TMD monolayers.The obtained effective model's dispersions are in good agreement with the DFT data around the specific wave vector,and the g-factors are consistent with experimental data.The VASP2KP package is available at https://github.com/zjwang11/VASP2KP.
基金supported by Science and Technology Project of SGCC(Research on Key Technologies for Fault Diagnosis and Early Warning of Intermittent Discharge in UHV GIS:524625200028).
文摘In view of the effective perception demand of PD(Partial Discharge)insulation defect of in-service GIS(Gas Insulated Switchgear),the external UHF(Ultra High Frequency,0.3−3 GHz)sensing technology for GIS PD insulation defect fromresin hole is studied in this paper.Firstly,rectangular waveguide theory is used to analyze the electromagnetic wave propagation characteristics from rectangular resin hole in GIS disc insulator.It is found that the energy of leaked electromagnetic wave mainly concentrates in the frequency band above 1 GHz.Then based on this frequency band characteristics,a cavity-backed bowtie sensor for GIS PD external detection is designed optimized by HFSS finite element software,in band of 1–3 GHz,its maximum VSWR is 7,the minimum is 1.1,the average is 2.9,and in band of 0.3–1 GHz,the maximum and minimum VSWR is 42.8 and 7,the average is 20.6.Finally,a sensor prototype is made,its sensitivity experiment is carried out on 220 kV GIS,experimental results show that the designed sensor can stably detect the PD electromagnetic wave signal leaked fromthe resin hole,and the signal energy in frequency band range is consistent with the theoretical analysis results.
文摘The American chestnut (<em>Castanea dentata</em>) was once a dominant tree species in the Appalachian Mountains and played a critical role in the ecological system. However, it was nearly eliminated by chestnut blight caused by the Ascomycetous fungus <em>Cryphonectria parasitica</em>. Identification of compounds specific to species and backcross hybrids may help further refine disease resistance breeding and testing. Phenolic compounds produced by plants are significant to their defense mechanisms against fungal pathogens. Therefore, an analytical platform has been developed to estimate the total phenolic content in leaf tissues of the American chestnut, Chinese chestnut (<em>Castanea mollissima</em>), and their backcross breeding generations (B<sub>3</sub>F<sub>2</sub> and B<sub>3</sub>F<sub>3</sub>) using the Folin-Ciocalteu reagent assay with UV/Vis spectrophotometry which may be used to predict blight resistance. Adsorption (765 nm) results from leaf tissue extraction in methanol/water (95%:5% v/v) and pH 2, show that the variations among these four tree species are significant (ANOVA p = 2.3 × 10<sup>-7</sup>). The kinetics of phenolic compound solid-liquid extraction was elaborated using Peleg, second order, Elovich, and power law models. In addition, extensive analysis using headspace solid phase microextraction (SPME) gas chromatography and mass spectrometry was conducted to identify volatile organic compounds (VOCs) from the leaf of American chestnut, Chinese chestnut, and their backcross hybrids B<sub>3</sub>F<sub>2</sub> and B<sub>3</sub>F<sub>3</sub>. A total of 67 VOCs were identified among all chestnut types. Many of the metabolites associated with the Chinese chestnut have been reported to have antifungal properties, whereas the native and hybrid American chestnut metabolites have not. Most of the antifungal metabolites showed the strongest efficacy towards the Ascomycota phylum. A partial least squares discriminant analysis (PLS-DA) model (R<sup>2</sup>X = 0.884, R<sup>2</sup>Y = 0.917, Q<sup>2</sup> = 0.584) differentiated chestnut species and hybrids within the first five principal component (PCs).
文摘目的:对比分析双眼三焦点IOL(AT LISA tri 839MP IOL)植入与一眼植入AT LISA tri 839MP IOL而另一眼植入非球面单焦点IOL患者术后视觉相关生活质量。方法:前瞻性临床研究。收集2018-05/2019-08在我院行白内障超声乳化联合三焦点AT LISA tri 839MP IOL植入的患者45例,按是否双眼植入AT LISA tri 839MP IOL分为两组,双眼植入者24例纳入双眼三焦点组;一眼植入AT LISA tri 839MP IOL而另一眼植入非球面单焦点IOL者21例纳入单眼三焦点组。术后3mo,检测两组患者裸眼视力及等效球镜度,并通过中国版视功能指数量表(VF-14-CN)评价视觉相关生活质量,评估日常生活脱镜率及满意度。结果:术后3mo,双眼三焦点组和单眼三焦点组患者双眼裸眼远、中、近视力均无明显差异(P>0.05),两组患者VF-14-CN问卷得分(96.2±0.50、92.43±1.32分)、日常生活脱镜率(96%、90%)及患者主观满意度(96%、95%)均无差异(P>0.05)。结论:双眼植入AT LISA tri 839MP IOL和一眼植入AT LISA tri 839MP IOL而另一眼植入非球面单焦点IOL的患者术后均具有良好的双眼裸眼远、中、近视力,术后视觉相关生活质量、脱镜率、满意度无明显差异。对一眼不适合植入三焦点IOL或既往一眼已植入非球面单焦点IOL的患者,可考虑另一眼行三焦点IOL植入以满足患者双眼全程视力的要求。
