Dietary parboiled rice(PR)has a low risk of disease,but little is known about the contribution of PR to the prevention of hyperlipidemia.The potential role and underlying mechanisms of PR in hyperlipidemia were evalua...Dietary parboiled rice(PR)has a low risk of disease,but little is known about the contribution of PR to the prevention of hyperlipidemia.The potential role and underlying mechanisms of PR in hyperlipidemia were evaluated in this study.Male C57BL/6J mice were fed with a normal diet,high-fat diet(HFD)containing refined rice(HFDRR)or PR(HFDPR).It was found that PR intervention improved lipid accumulation in mice.Transcriptomic data analysis revealed that 27 genes were up-regulated(mostly involved in lipid breakdown)and 86 genes were down-regulated(mostly involved in inflammatory responses)in the HFDPR group compared to the HFDRR group.And 15 differentially expressed genes(DEGs)were validated by quantitative real-time PCR(RT-qPCR),while protein interaction network showed that protein tyrosine phosphatase receptor type C(PTPRC)has a central role.The gut microbiota of mice was also altered after different dietary treatments,with higher ratio of Firmicutes and Bacteroidetes,increased abundances of Ruminococcaceae,Lachnospiraceae,Christensenellaceae,Porphyromonadaceae,Rikenellaceae and Prevotellaceae,and decreased abundances of Lactobacillaceae,Peptostreptococcaceae,Erysipelotrichaceae and Actinobacteria in the HFDRR group.In addition,it was observed that PPAR signaling pathway may act as a bridge between DEGs and differential gut microbiota.These results suggested that PR can prevent hyperlipidemia by modulating liver genes and gut microbiota.展开更多
Six hydrophobic peptidyl and four amino acid (L-Val, L-Leu,L-Phe, L-Gly) derivatives of 5-fluorouracil(5-FU) were synthesized.The structure of these compounds were characterized by ~1H-NMR, IR,UV spectra and elemental...Six hydrophobic peptidyl and four amino acid (L-Val, L-Leu,L-Phe, L-Gly) derivatives of 5-fluorouracil(5-FU) were synthesized.The structure of these compounds were characterized by ~1H-NMR, IR,UV spectra and elemental analysis. The antitumor activity was test-ed against EAC cells in vitro.展开更多
Backgroud:Danggui Buxue decoction(DBD),a classical prescription in traditional Chinese medicine,has been found to have protective effect on bleomycin-induced pulmonary fibrosis in rats by reducing alveolar inflammatio...Backgroud:Danggui Buxue decoction(DBD),a classical prescription in traditional Chinese medicine,has been found to have protective effect on bleomycin-induced pulmonary fibrosis in rats by reducing alveolar inflammation and fibrosis.However,the biological activity of individual chemical components and mechanism of action of whole formula are not clear.Methods:Potential targets of active ingredients of DBD were collected through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and SymMap database.Target genes related to idiopathic pulmonary fibrosis were obtained from the Online Mendelian Inheritance in Man database,Therapeutic Targets Database and Gkb database.Then,the common targets were obtained by overlapping the potential targets of active ingredients in DBD and diseases related targets.The selected targets were subjected to Kyoto Encyclopedia of Genes and Genomes signaling pathway and Gene Ontology analysis,and the network map of active component-target-pathway was established using Cytoscape 3.7.1 software.The active components of DBD with most targets were selected for fibrosis-related marker verification.The mRNA and protein expression of fibrosis markers,α-smooth muscle actin,collagen 1 and fibronectin,were detected in TGF-β1-induced fibroblast cell line after treatment with the active components.Results:The 14 active ingredients,such as quercetin and kaempferol,were screened from DBD.It acts on 26 targets like estrogen receptor 2 and prostaglandin-endoperoxide synthase 2,and mainly involves 38 signaling pathways such as cell inflammation and autophagy.Kaempferol and quercetin are the two compounds with the highest network regulation,which can inhibit the transformation of fibroblasts into myofibroblasts and reduce the expression of fibrosis markersα-smooth muscle actin,collagen 1 and fibronectin.Conclusion:The integration mode of multi-component,multi-target,multi-channel and mechanism of DBD in the treatment of idiopathic pulmonary fibrosis are predicted by means of network pharmacology.Our study could indicate the direction of further anti-fibrotic mechanism research.展开更多
[Objectives]This study was conducted to establish characteristic chromatograms of of the volatile oil of Xinyi Biyan Pills by gas chromatography,discover possible problems in the production processes of different manu...[Objectives]This study was conducted to establish characteristic chromatograms of of the volatile oil of Xinyi Biyan Pills by gas chromatography,discover possible problems in the production processes of different manufacturers,and further improve the quality control methods.[Methods]The volatile oil in samples was extracted and tested by gas chromatography to collect chromatograms,which were analyze and evaluated by the similarity evaluation software of chromatographic fingerprints of traditional Chinese medicine.[Results]Nineteen common peaks were calibrated in the characteristic chromatograms;and the characteristic chromatograms of samples produced by different manufacturers were obviously different.[Conclusions]Controlling the volatile components in Xinyi Biyan Pills by the established characteristic chromatograms of GC is accurate and feasible,and can be used as a quality control method for Xinyi Biyan Pills.展开更多
Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H ...Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H vessels have recently been identified as a bone-specific vascular subtype that supports osteogenesis.Here,we show that metformin accelerated fracture healing in both osteoporotic and normal mice.Moreover,metformin promoted angiogenesis in vitro under hypoxia as well as type H vessel formation throughout fracture healing.Mechanistically,metformin increased the expression of HIF-1α,an important positive regulator of type H vessel formation,by inhibiting the expression of YAP1/TAZ in calluses and hypoxia-cultured human microvascular endothelial cells(HMECs).The results of HIF-1αor YAP1/TAZ interference in hypoxia-cultured HMECs using si RNA further suggested that the enhancement of HIF-1αand its target genes by metformin is primarily through YAP1/TAZ inhibition.Finally,overexpression of YAP1/TAZ partially counteracted the effect of metformin in promoting type H vessel-induced angiogenesis-osteogenesis coupling during fracture repair.In summary,our findings suggest that metformin has the potential to be a therapeutic agent for fractures by promoting type H vessel formation through YAP1/TAZ inhibition.展开更多
Stochastic fractional differential systems are important and useful in the mathematics,physics,and engineering fields.However,the determination of their probabilistic responses is difficult due to their non-Markovian ...Stochastic fractional differential systems are important and useful in the mathematics,physics,and engineering fields.However,the determination of their probabilistic responses is difficult due to their non-Markovian property.The recently developed globally-evolving-based generalized density evolution equation(GE-GDEE),which is a unified partial differential equation(PDE)governing the transient probability density function(PDF)of a generic path-continuous process,including non-Markovian ones,provides a feasible tool to solve this problem.In the paper,the GE-GDEE for multi-dimensional linear fractional differential systems subject to Gaussian white noise is established.In particular,it is proved that in the GE-GDEE corresponding to the state-quantities of interest,the intrinsic drift coefficient is a time-varying linear function,and can be analytically determined.In this sense,an alternative low-dimensional equivalent linear integer-order differential system with exact closed-form coefficients for the original highdimensional linear fractional differential system can be constructed such that their transient PDFs are identical.Specifically,for a multi-dimensional linear fractional differential system,if only one or two quantities are of interest,GE-GDEE is only in one or two dimensions,and the surrogate system would be a one-or two-dimensional linear integer-order system.Several examples are studied to assess the merit of the proposed method.Though presently the closed-form intrinsic drift coefficient is only available for linear stochastic fractional differential systems,the findings in the present paper provide a remarkable demonstration on the existence and eligibility of GE-GDEE for the case that the original high-dimensional system itself is non-Markovian,and provide insights for the physical-mechanism-informed determination of intrinsic drift and diffusion coefficients of GE-GDEE of more generic complex nonlinear systems.展开更多
Microscopic vision has been widely applied in precision assembly.To achieve sufficiently high resolution in measurements for precision assembly when the sizes of the parts involved exceed the field of view of the visi...Microscopic vision has been widely applied in precision assembly.To achieve sufficiently high resolution in measurements for precision assembly when the sizes of the parts involved exceed the field of view of the vision system,an image mosaic technique must be used.In this paper,a method for constructing an image mosaic with non-overlapping areas with enhanced efficiency is proposed.First,an image mosaic model for the part is created using a geometric model of the measurement system installed on a X-Y-Z precision stages with high repeatability,and a path for image acquisition is established.Second,images are captured along the same path for a specified calibration plate,and an entire image is formed based on the given model.The measurement results obtained from the specified calibration plate are utilized to identify mosaic errors and apply compensation for the part requiring measurement.Experimental results show that the maximum error is less than 4μm for a camera with pixel equivalent 2.46μm,thereby demonstrating the accuracy of the proposed method.This image mosaic technique with non-overlapping regions can simplify image acquisition and reduce the workload involved in constructing an image mosaic.展开更多
<strong>Background</strong><span style="font-family:Verdana;"><strong>:</strong></span><b><span style="font-family:""> </span></b>&l...<strong>Background</strong><span style="font-family:Verdana;"><strong>:</strong></span><b><span style="font-family:""> </span></b><span style="font-family:""><span style="font-family:Verdana;">Malignant granular cell tumor (GCT) is extremely rare. Malignant GCT with EP300 gene mutation in the bladder has not been reported in the literature. </span><b><span style="font-family:Verdana;">Case Presentation</span></b></span><b><span style="font-family:Verdana;">:</span></b><span style="font-family:""><span style="font-family:Verdana;"> We report a special case of 45-year-old female with malignant GCT of the bladder. Pathological examination showed that the mass was 11 × 11 × 4.5 cm in size, involved in the bladder’s posterior wall. Under the microscope, the tumor cells were arranged in the shape of a nest or cord to infiltrate the bladder’s wall. The tumor cells were pleomorphic, red-stained granular within the cytoplasm, with increased nuclear/cyto</span><span style="font-family:Verdana;">plasmic ratio, vacuolar nuclei, and obvious nucleoli. The tumor cells were</span> <span style="font-family:Verdana;">showed obvious nuclear atypia, and the mitosis was more than 5/50HPF. </span><span style="font-family:Verdana;">Coagulative necrosis was widely showed within the tumor. Immunohistochemi</span><span><span style="font-family:Verdana;">stry (IHC) showed that S-100, NSE, CD68, CR, </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-AT, and TFE-3 were</span></span><span style="font-family:Verdana;"> strongly </span><span style="font-family:Verdana;">positive, and the Ki-67 proliferation index was around 15%. The</span><span style="font-family:Verdana;"> next-genera</span><span style="font-family:Verdana;">tion high throughput sequencing indicated that EP300 gene was missense</span><span style="font-family:Verdana;"> mutated (c.457A > G) with 33% mutation abundance, and genes of DPYD (c.1627A > G), ERCC1 (c.354T > C), NQO1 (c.559C > T), TPMT (c.719A > G) and XRCC1 (c.1196A > G) were polymorphic mutated. The patient died after three months of the second surgical treatment. </span><b><span style="font-family:Verdana;">Conclusions</span></b></span><b><span style="font-family:Verdana;">:</span></b><b><span style="font-family:""> </span></b><span style="font-family:Verdana;">We report for the first time a primary bladder malignant GCM accompanied by mutations in special driving genes such as EP300. We also conducted a comprehensive literature review and an in-depth discussion.</span>展开更多
Source and occurrence of Excess Coalbed Methane is a long-term concern research topic in Coal Geology and Structural Geology. Since it is essential to understand the outburst mechanism of coal gas, and to support the ...Source and occurrence of Excess Coalbed Methane is a long-term concern research topic in Coal Geology and Structural Geology. Since it is essential to understand the outburst mechanism of coal gas, and to support the coalbed methane development projects as the theoretical basis. We found in the study that, huge imparity is behind the evolutionary trend on molecular structure and the mechanism of influence from different deformation. The thesis demonstrates its probable routes of gas evolution according to distinct deformation mechanisms of coal. In the role of brittle deformation mechanism, a rapidly formed advantage rupture surface along with sliding motion from which has worked on coal. As another result, mechanical energy has transformed into friction and kinetic energy during the process. Kinetic energy increases simultaneously, which brings some results, that the new generated gas molecule. While the chemical structure of coal remains in a steady-state and do not react easily an outburst with gas. Mechanical energy turns into strain energy through its ductile deformation mechanism. The dislocation or lamellar slip made disordered between the constitutional units of aromatic rings and aromatic lamellas, as soon as secondary structural defects created. On another hand, molecular motion accelerates and splits off the small molecular on the side chain, due to the dissociation of aromatic nucleus;CH<sub>4 </sub>gas molecular was generated and placed in the secondary structural defect of coal, along with a great deal of strain energy in non-steady-state. By breaking away the balance maintaining terms, huge strain energy releases suddenly, small moleculars are free from the secondary structure defect, react outburst with gas. Furthermore to extend the discussion of the conventional physical ideas on coal absorb gas, according to the phenomenon of exceeded CBM, the gas molecular has a significant chance existing in a low bond energy of chemical bonds of coal structure.展开更多
Objective:Anlotinib hydrochloride is a multitarget tyrosine kinase inhibitor that targets vascular endothelial growth factor receptor,fibroblast growth factor receptor,platelet-derived growth factor receptor,c-Kit,and...Objective:Anlotinib hydrochloride is a multitarget tyrosine kinase inhibitor that targets vascular endothelial growth factor receptor,fibroblast growth factor receptor,platelet-derived growth factor receptor,c-Kit,and c-MET;therefore,it exhibits both antitumor and anti-angiogenetic activities.A phase III trial has shown that anlotinib improved progression-free survival(PFS)and overall survival(OS)in patients with advanced non-small cell lung cancer(NSCLC),who presented with progressive disease or intolerance after standard chemotherapy.This study aimed to analyze the characteristics of patients receiving anlotinib treatment to determine the dominant populations who are fit for the treatment.Methods:Data were collected from March 2015 to January 2017 from a randomized,double-blind,placebo-controlled,multicenter,phase III trial of anlotinib(ALTER0303).A total of 437 patients were enrolled and randomly allocated(2:1)to the anlotinib and placebo groups.Kaplan–Meier analysis and log-rank test were performed to compare PFS and OS.Cox proportional hazards model was adopted for multivariate prognostic analysis.Results:Multivariate analysis indicated that high post-therapeutic peripheral blood granulocyte/lymphocyte ratio and elevated alkaline phosphatase levels were independent risk factors for PFS.Meanwhile,elevated thyroid-stimulating hormone,blood glucose,and triglyceride levels;hypertension;and hand–foot syndrome were independent protective factors of PFS.High posttherapeutic peripheral blood granulocyte/lymphocyte ratio,an Eastern Cooperative Oncology Group(ECOG)score≥2,and the sum of the maximal target lesion length at baseline were independent risk factors of OS,and hypertriglyceridemia was an independent protective factor of OS.Conclusions:This study preliminarily explored the possible factors that affected PFS and OS after anlotinib treatment in patients with advanced refractory NSCLC,and the baseline characteristics of the therapeutically dominant populations were then identified.展开更多
目的:分析阿帕替尼治疗复发性和进展性韧带样纤维瘤(desmoid fibromatosis,DF)的疗效和安全性。方法:收集2017年10月至2020年9月就诊于四川大学华西医院的复发性或进展性韧带样纤维瘤患者37例,接受靶向药物阿帕替尼治疗,起始剂量250 mg...目的:分析阿帕替尼治疗复发性和进展性韧带样纤维瘤(desmoid fibromatosis,DF)的疗效和安全性。方法:收集2017年10月至2020年9月就诊于四川大学华西医院的复发性或进展性韧带样纤维瘤患者37例,接受靶向药物阿帕替尼治疗,起始剂量250 mg/d,根据药物不良反应调整剂量。结果:无完全缓解病例,17例(45.9%)为部分缓解,18例(48.6%)为疾病稳定,2例(5.4%)为疾病进展,客观有效率为45.9%,疾病控制率为94.6%。患者的关节活动度(rang of motion,ROM)、视觉模拟评分(visual analog scale,VAS)、上肢功能评定表(disabilities of the arm,shoulder and hand,DASH)评分、髋关节Harris评分及美国特种外科医院(HSS)评分均较治疗前有明显改善,且差异具有统计学意义(P<0.05)。常见的不良反应为手足综合征(37.8%)、胃肠反应(29.7%)和月经紊乱(21.6%)。结论:靶向药物阿帕替尼对复发性和进展性DF有一定的疗效,且不良反应可耐受,可作为复发性和进展性韧带样纤维瘤治疗的新途径。展开更多
基金financially supported by Key Project of State Key R&D Program,China (2022YFF1100200)the Program for Science&Technology Innovation Platform of Hunan Province (2019TP102)+3 种基金Natural Science Foundation of Hunan Province (2021JJ31075,2019JJ50984)Natural Science Foundation of Changsha City (kq2014275)Scientific Innovation Fund for Postgraduates of Central South University of Forestry and Technology (CX20200699,CX202102067)Postgraduate Scientific Research Innovation Project of Hunan Province (CX20201018,CX20210899,CX20220701 and CX20220720)。
文摘Dietary parboiled rice(PR)has a low risk of disease,but little is known about the contribution of PR to the prevention of hyperlipidemia.The potential role and underlying mechanisms of PR in hyperlipidemia were evaluated in this study.Male C57BL/6J mice were fed with a normal diet,high-fat diet(HFD)containing refined rice(HFDRR)or PR(HFDPR).It was found that PR intervention improved lipid accumulation in mice.Transcriptomic data analysis revealed that 27 genes were up-regulated(mostly involved in lipid breakdown)and 86 genes were down-regulated(mostly involved in inflammatory responses)in the HFDPR group compared to the HFDRR group.And 15 differentially expressed genes(DEGs)were validated by quantitative real-time PCR(RT-qPCR),while protein interaction network showed that protein tyrosine phosphatase receptor type C(PTPRC)has a central role.The gut microbiota of mice was also altered after different dietary treatments,with higher ratio of Firmicutes and Bacteroidetes,increased abundances of Ruminococcaceae,Lachnospiraceae,Christensenellaceae,Porphyromonadaceae,Rikenellaceae and Prevotellaceae,and decreased abundances of Lactobacillaceae,Peptostreptococcaceae,Erysipelotrichaceae and Actinobacteria in the HFDRR group.In addition,it was observed that PPAR signaling pathway may act as a bridge between DEGs and differential gut microbiota.These results suggested that PR can prevent hyperlipidemia by modulating liver genes and gut microbiota.
文摘Six hydrophobic peptidyl and four amino acid (L-Val, L-Leu,L-Phe, L-Gly) derivatives of 5-fluorouracil(5-FU) were synthesized.The structure of these compounds were characterized by ~1H-NMR, IR,UV spectra and elemental analysis. The antitumor activity was test-ed against EAC cells in vitro.
文摘Backgroud:Danggui Buxue decoction(DBD),a classical prescription in traditional Chinese medicine,has been found to have protective effect on bleomycin-induced pulmonary fibrosis in rats by reducing alveolar inflammation and fibrosis.However,the biological activity of individual chemical components and mechanism of action of whole formula are not clear.Methods:Potential targets of active ingredients of DBD were collected through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and SymMap database.Target genes related to idiopathic pulmonary fibrosis were obtained from the Online Mendelian Inheritance in Man database,Therapeutic Targets Database and Gkb database.Then,the common targets were obtained by overlapping the potential targets of active ingredients in DBD and diseases related targets.The selected targets were subjected to Kyoto Encyclopedia of Genes and Genomes signaling pathway and Gene Ontology analysis,and the network map of active component-target-pathway was established using Cytoscape 3.7.1 software.The active components of DBD with most targets were selected for fibrosis-related marker verification.The mRNA and protein expression of fibrosis markers,α-smooth muscle actin,collagen 1 and fibronectin,were detected in TGF-β1-induced fibroblast cell line after treatment with the active components.Results:The 14 active ingredients,such as quercetin and kaempferol,were screened from DBD.It acts on 26 targets like estrogen receptor 2 and prostaglandin-endoperoxide synthase 2,and mainly involves 38 signaling pathways such as cell inflammation and autophagy.Kaempferol and quercetin are the two compounds with the highest network regulation,which can inhibit the transformation of fibroblasts into myofibroblasts and reduce the expression of fibrosis markersα-smooth muscle actin,collagen 1 and fibronectin.Conclusion:The integration mode of multi-component,multi-target,multi-channel and mechanism of DBD in the treatment of idiopathic pulmonary fibrosis are predicted by means of network pharmacology.Our study could indicate the direction of further anti-fibrotic mechanism research.
基金Guangxi Key R&D Program Project (GK AB19110027).
文摘[Objectives]This study was conducted to establish characteristic chromatograms of of the volatile oil of Xinyi Biyan Pills by gas chromatography,discover possible problems in the production processes of different manufacturers,and further improve the quality control methods.[Methods]The volatile oil in samples was extracted and tested by gas chromatography to collect chromatograms,which were analyze and evaluated by the similarity evaluation software of chromatographic fingerprints of traditional Chinese medicine.[Results]Nineteen common peaks were calibrated in the characteristic chromatograms;and the characteristic chromatograms of samples produced by different manufacturers were obviously different.[Conclusions]Controlling the volatile components in Xinyi Biyan Pills by the established characteristic chromatograms of GC is accurate and feasible,and can be used as a quality control method for Xinyi Biyan Pills.
基金supported by the National Natural Science Foundation of China (Grant Nos.81874006,82172399,81902222,82060395,81902277,82072504,82000845)the Hunan Province Natural Science Foundation of China (Grant Nos.2020JJ4928,2020JJ4897,2021JJ30038,2021JJ40492)the Independent Exploration and Innovation Project of Central South University (Grant Nos.2020zzts255)。
文摘Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H vessels have recently been identified as a bone-specific vascular subtype that supports osteogenesis.Here,we show that metformin accelerated fracture healing in both osteoporotic and normal mice.Moreover,metformin promoted angiogenesis in vitro under hypoxia as well as type H vessel formation throughout fracture healing.Mechanistically,metformin increased the expression of HIF-1α,an important positive regulator of type H vessel formation,by inhibiting the expression of YAP1/TAZ in calluses and hypoxia-cultured human microvascular endothelial cells(HMECs).The results of HIF-1αor YAP1/TAZ interference in hypoxia-cultured HMECs using si RNA further suggested that the enhancement of HIF-1αand its target genes by metformin is primarily through YAP1/TAZ inhibition.Finally,overexpression of YAP1/TAZ partially counteracted the effect of metformin in promoting type H vessel-induced angiogenesis-osteogenesis coupling during fracture repair.In summary,our findings suggest that metformin has the potential to be a therapeutic agent for fractures by promoting type H vessel formation through YAP1/TAZ inhibition.
基金The supports of the National Natural Science Foundation of China(Grant Nos.51725804 and U1711264)the Research Fund for State Key Laboratories of Ministry of Science and Technology of China(SLDRCE19-B-23)the Shanghai Post-Doctoral Excellence Program(2022558)。
文摘Stochastic fractional differential systems are important and useful in the mathematics,physics,and engineering fields.However,the determination of their probabilistic responses is difficult due to their non-Markovian property.The recently developed globally-evolving-based generalized density evolution equation(GE-GDEE),which is a unified partial differential equation(PDE)governing the transient probability density function(PDF)of a generic path-continuous process,including non-Markovian ones,provides a feasible tool to solve this problem.In the paper,the GE-GDEE for multi-dimensional linear fractional differential systems subject to Gaussian white noise is established.In particular,it is proved that in the GE-GDEE corresponding to the state-quantities of interest,the intrinsic drift coefficient is a time-varying linear function,and can be analytically determined.In this sense,an alternative low-dimensional equivalent linear integer-order differential system with exact closed-form coefficients for the original highdimensional linear fractional differential system can be constructed such that their transient PDFs are identical.Specifically,for a multi-dimensional linear fractional differential system,if only one or two quantities are of interest,GE-GDEE is only in one or two dimensions,and the surrogate system would be a one-or two-dimensional linear integer-order system.Several examples are studied to assess the merit of the proposed method.Though presently the closed-form intrinsic drift coefficient is only available for linear stochastic fractional differential systems,the findings in the present paper provide a remarkable demonstration on the existence and eligibility of GE-GDEE for the case that the original high-dimensional system itself is non-Markovian,and provide insights for the physical-mechanism-informed determination of intrinsic drift and diffusion coefficients of GE-GDEE of more generic complex nonlinear systems.
基金supported by the Liaoning Revitalization Talents Program(Grant No.XLYC2002020)the Major Project of Basic Scientific Research of Chinese Ministry(Grant No.JCYK2016205A003).
文摘Microscopic vision has been widely applied in precision assembly.To achieve sufficiently high resolution in measurements for precision assembly when the sizes of the parts involved exceed the field of view of the vision system,an image mosaic technique must be used.In this paper,a method for constructing an image mosaic with non-overlapping areas with enhanced efficiency is proposed.First,an image mosaic model for the part is created using a geometric model of the measurement system installed on a X-Y-Z precision stages with high repeatability,and a path for image acquisition is established.Second,images are captured along the same path for a specified calibration plate,and an entire image is formed based on the given model.The measurement results obtained from the specified calibration plate are utilized to identify mosaic errors and apply compensation for the part requiring measurement.Experimental results show that the maximum error is less than 4μm for a camera with pixel equivalent 2.46μm,thereby demonstrating the accuracy of the proposed method.This image mosaic technique with non-overlapping regions can simplify image acquisition and reduce the workload involved in constructing an image mosaic.
文摘<strong>Background</strong><span style="font-family:Verdana;"><strong>:</strong></span><b><span style="font-family:""> </span></b><span style="font-family:""><span style="font-family:Verdana;">Malignant granular cell tumor (GCT) is extremely rare. Malignant GCT with EP300 gene mutation in the bladder has not been reported in the literature. </span><b><span style="font-family:Verdana;">Case Presentation</span></b></span><b><span style="font-family:Verdana;">:</span></b><span style="font-family:""><span style="font-family:Verdana;"> We report a special case of 45-year-old female with malignant GCT of the bladder. Pathological examination showed that the mass was 11 × 11 × 4.5 cm in size, involved in the bladder’s posterior wall. Under the microscope, the tumor cells were arranged in the shape of a nest or cord to infiltrate the bladder’s wall. The tumor cells were pleomorphic, red-stained granular within the cytoplasm, with increased nuclear/cyto</span><span style="font-family:Verdana;">plasmic ratio, vacuolar nuclei, and obvious nucleoli. The tumor cells were</span> <span style="font-family:Verdana;">showed obvious nuclear atypia, and the mitosis was more than 5/50HPF. </span><span style="font-family:Verdana;">Coagulative necrosis was widely showed within the tumor. Immunohistochemi</span><span><span style="font-family:Verdana;">stry (IHC) showed that S-100, NSE, CD68, CR, </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-AT, and TFE-3 were</span></span><span style="font-family:Verdana;"> strongly </span><span style="font-family:Verdana;">positive, and the Ki-67 proliferation index was around 15%. The</span><span style="font-family:Verdana;"> next-genera</span><span style="font-family:Verdana;">tion high throughput sequencing indicated that EP300 gene was missense</span><span style="font-family:Verdana;"> mutated (c.457A > G) with 33% mutation abundance, and genes of DPYD (c.1627A > G), ERCC1 (c.354T > C), NQO1 (c.559C > T), TPMT (c.719A > G) and XRCC1 (c.1196A > G) were polymorphic mutated. The patient died after three months of the second surgical treatment. </span><b><span style="font-family:Verdana;">Conclusions</span></b></span><b><span style="font-family:Verdana;">:</span></b><b><span style="font-family:""> </span></b><span style="font-family:Verdana;">We report for the first time a primary bladder malignant GCM accompanied by mutations in special driving genes such as EP300. We also conducted a comprehensive literature review and an in-depth discussion.</span>
文摘Source and occurrence of Excess Coalbed Methane is a long-term concern research topic in Coal Geology and Structural Geology. Since it is essential to understand the outburst mechanism of coal gas, and to support the coalbed methane development projects as the theoretical basis. We found in the study that, huge imparity is behind the evolutionary trend on molecular structure and the mechanism of influence from different deformation. The thesis demonstrates its probable routes of gas evolution according to distinct deformation mechanisms of coal. In the role of brittle deformation mechanism, a rapidly formed advantage rupture surface along with sliding motion from which has worked on coal. As another result, mechanical energy has transformed into friction and kinetic energy during the process. Kinetic energy increases simultaneously, which brings some results, that the new generated gas molecule. While the chemical structure of coal remains in a steady-state and do not react easily an outburst with gas. Mechanical energy turns into strain energy through its ductile deformation mechanism. The dislocation or lamellar slip made disordered between the constitutional units of aromatic rings and aromatic lamellas, as soon as secondary structural defects created. On another hand, molecular motion accelerates and splits off the small molecular on the side chain, due to the dissociation of aromatic nucleus;CH<sub>4 </sub>gas molecular was generated and placed in the secondary structural defect of coal, along with a great deal of strain energy in non-steady-state. By breaking away the balance maintaining terms, huge strain energy releases suddenly, small moleculars are free from the secondary structure defect, react outburst with gas. Furthermore to extend the discussion of the conventional physical ideas on coal absorb gas, according to the phenomenon of exceeded CBM, the gas molecular has a significant chance existing in a low bond energy of chemical bonds of coal structure.
文摘Objective:Anlotinib hydrochloride is a multitarget tyrosine kinase inhibitor that targets vascular endothelial growth factor receptor,fibroblast growth factor receptor,platelet-derived growth factor receptor,c-Kit,and c-MET;therefore,it exhibits both antitumor and anti-angiogenetic activities.A phase III trial has shown that anlotinib improved progression-free survival(PFS)and overall survival(OS)in patients with advanced non-small cell lung cancer(NSCLC),who presented with progressive disease or intolerance after standard chemotherapy.This study aimed to analyze the characteristics of patients receiving anlotinib treatment to determine the dominant populations who are fit for the treatment.Methods:Data were collected from March 2015 to January 2017 from a randomized,double-blind,placebo-controlled,multicenter,phase III trial of anlotinib(ALTER0303).A total of 437 patients were enrolled and randomly allocated(2:1)to the anlotinib and placebo groups.Kaplan–Meier analysis and log-rank test were performed to compare PFS and OS.Cox proportional hazards model was adopted for multivariate prognostic analysis.Results:Multivariate analysis indicated that high post-therapeutic peripheral blood granulocyte/lymphocyte ratio and elevated alkaline phosphatase levels were independent risk factors for PFS.Meanwhile,elevated thyroid-stimulating hormone,blood glucose,and triglyceride levels;hypertension;and hand–foot syndrome were independent protective factors of PFS.High posttherapeutic peripheral blood granulocyte/lymphocyte ratio,an Eastern Cooperative Oncology Group(ECOG)score≥2,and the sum of the maximal target lesion length at baseline were independent risk factors of OS,and hypertriglyceridemia was an independent protective factor of OS.Conclusions:This study preliminarily explored the possible factors that affected PFS and OS after anlotinib treatment in patients with advanced refractory NSCLC,and the baseline characteristics of the therapeutically dominant populations were then identified.
文摘目的:分析阿帕替尼治疗复发性和进展性韧带样纤维瘤(desmoid fibromatosis,DF)的疗效和安全性。方法:收集2017年10月至2020年9月就诊于四川大学华西医院的复发性或进展性韧带样纤维瘤患者37例,接受靶向药物阿帕替尼治疗,起始剂量250 mg/d,根据药物不良反应调整剂量。结果:无完全缓解病例,17例(45.9%)为部分缓解,18例(48.6%)为疾病稳定,2例(5.4%)为疾病进展,客观有效率为45.9%,疾病控制率为94.6%。患者的关节活动度(rang of motion,ROM)、视觉模拟评分(visual analog scale,VAS)、上肢功能评定表(disabilities of the arm,shoulder and hand,DASH)评分、髋关节Harris评分及美国特种外科医院(HSS)评分均较治疗前有明显改善,且差异具有统计学意义(P<0.05)。常见的不良反应为手足综合征(37.8%)、胃肠反应(29.7%)和月经紊乱(21.6%)。结论:靶向药物阿帕替尼对复发性和进展性DF有一定的疗效,且不良反应可耐受,可作为复发性和进展性韧带样纤维瘤治疗的新途径。
