The basal activity of JNK is low in normal growing cells and inactivated JNK targets p53 for ubiquitination. To elucidate if the C-terminal part of JNK is responsible for its binding to p53, the low background tet-off...The basal activity of JNK is low in normal growing cells and inactivated JNK targets p53 for ubiquitination. To elucidate if the C-terminal part of JNK is responsible for its binding to p53, the low background tet-off inducible NIH3T3 cell line was selected by luciferase reporter gene and a double stable C-JNK Aa (203-424) cell line was established. After withdrawing tetracycline, the C-JNK fragment expression was induced and cell growth was dramati- cally inhibited 24 h later. However, the expresion of p53 was found to be increased after the induction of C-JNK fragment, evaluated by transfecting p21waf-luciferase reporter genes. Our further studies showed that C-JNK fragment could form complex with p53 both in vivo and in vitro. Induction of C-JNK fragment in vivo can increase p53 stability by inhibiting p53 ubiquitination.展开更多
A CO2 laser-based phase contrast imaging(PCI) diagnostic has been developed on HL-2A tokamak.It can detect line integrated plasma density fluctuations by measuring the phase shift of laser beam after being scattered b...A CO2 laser-based phase contrast imaging(PCI) diagnostic has been developed on HL-2A tokamak.It can detect line integrated plasma density fluctuations by measuring the phase shift of laser beam after being scattered by the bulk plasma.The diagnosed radial region ranges from ρ≡r/a =0.625 to 0.7.32-channel HgCdTe detectors with alternative-current biased amplifiers are arranged in line at the imaging plane of the optical path.This PCI is able to diagnose density fluctuations with wavenumbers ranging from 2 to 15 cm-1 and the time resolution is better than 2 μs.The first experimental data were achieved in 2018 spring campaign of HL-2A tokamak.High performance is confirmed in different discharging configurations and makes it a keen tool in broadband turbulence investigations.展开更多
Phosphorus(P)is involved in various biochemical reactions in plant growth,so it is beneficial to plants growing in soils contaminated by metals,including cadmium(Cd).However,few studies have reported on the mechanisti...Phosphorus(P)is involved in various biochemical reactions in plant growth,so it is beneficial to plants growing in soils contaminated by metals,including cadmium(Cd).However,few studies have reported on the mechanistic roles of P in mitigating Cd toxicity to ryegrass root,and especially in alleviating the disruption of the mitochondrial function of living cells.In this study,the physiological and biochemical mechanisms associated with ryegrass growth under various Cd and P treatments were investigated using leaching and pot systems.The concentration of Cd in soil leachates showed a significantly positive relationship with redox potential(P<0.05),but negative relationship(P<0.05)with leachate pH values and dissolved organic carbon(DOC),indicating that exogenous P addition(as H_(2)PO_(4)*)may decrease Cd leaching from contaminated soil.Compared to the control(without P addition),the cumulative Cd content was reduced by 53.3%and 64.5%in the soil leachate with exogenous P application(20 mg/L and 80 mg/L),respectively.Notably,application of P decreased the Cd concentrations in the symplastic fractions and increased the Cd concentrations in the apoplastic fractions in root tips,which may help to alleviate Cd stress to the protoplast.Moreover,exogenous P was found to play a positive role in mitochondrial function and Ca^(2+)variation in root cells under Cd stress,which provides novel insights into the mechanisms of exogenous P in alleviating plant Cd injury.展开更多
基金supported by National Natural Science Foundation of China(No.30270556)The National Basic Research Program(No.2002CB513004).
文摘The basal activity of JNK is low in normal growing cells and inactivated JNK targets p53 for ubiquitination. To elucidate if the C-terminal part of JNK is responsible for its binding to p53, the low background tet-off inducible NIH3T3 cell line was selected by luciferase reporter gene and a double stable C-JNK Aa (203-424) cell line was established. After withdrawing tetracycline, the C-JNK fragment expression was induced and cell growth was dramati- cally inhibited 24 h later. However, the expresion of p53 was found to be increased after the induction of C-JNK fragment, evaluated by transfecting p21waf-luciferase reporter genes. Our further studies showed that C-JNK fragment could form complex with p53 both in vivo and in vitro. Induction of C-JNK fragment in vivo can increase p53 stability by inhibiting p53 ubiquitination.
基金supported by the National Key Research and Development Program of China (No.2017YFE0300405)National Natural Science Foundation of China (Nos.11875124, 11705052, 11575055 and 11611130164)the National Magnetic Confinement Fusion Science Program of China (No.2015GB120002)
文摘A CO2 laser-based phase contrast imaging(PCI) diagnostic has been developed on HL-2A tokamak.It can detect line integrated plasma density fluctuations by measuring the phase shift of laser beam after being scattered by the bulk plasma.The diagnosed radial region ranges from ρ≡r/a =0.625 to 0.7.32-channel HgCdTe detectors with alternative-current biased amplifiers are arranged in line at the imaging plane of the optical path.This PCI is able to diagnose density fluctuations with wavenumbers ranging from 2 to 15 cm-1 and the time resolution is better than 2 μs.The first experimental data were achieved in 2018 spring campaign of HL-2A tokamak.High performance is confirmed in different discharging configurations and makes it a keen tool in broadband turbulence investigations.
基金supported by the Natural Science Foundation of Jiangsu Province (No.BK20210751)China Postdoctoral Research Foundation (No.2022M711389)the Carbon Peak and Carbon Neutrality Technology Innovation Foundation of Jiangsu Province (No.BK20220030)。
文摘Phosphorus(P)is involved in various biochemical reactions in plant growth,so it is beneficial to plants growing in soils contaminated by metals,including cadmium(Cd).However,few studies have reported on the mechanistic roles of P in mitigating Cd toxicity to ryegrass root,and especially in alleviating the disruption of the mitochondrial function of living cells.In this study,the physiological and biochemical mechanisms associated with ryegrass growth under various Cd and P treatments were investigated using leaching and pot systems.The concentration of Cd in soil leachates showed a significantly positive relationship with redox potential(P<0.05),but negative relationship(P<0.05)with leachate pH values and dissolved organic carbon(DOC),indicating that exogenous P addition(as H_(2)PO_(4)*)may decrease Cd leaching from contaminated soil.Compared to the control(without P addition),the cumulative Cd content was reduced by 53.3%and 64.5%in the soil leachate with exogenous P application(20 mg/L and 80 mg/L),respectively.Notably,application of P decreased the Cd concentrations in the symplastic fractions and increased the Cd concentrations in the apoplastic fractions in root tips,which may help to alleviate Cd stress to the protoplast.Moreover,exogenous P was found to play a positive role in mitochondrial function and Ca^(2+)variation in root cells under Cd stress,which provides novel insights into the mechanisms of exogenous P in alleviating plant Cd injury.
