To investigate whether secretion of soluble human leukocyte antigen- G (HLA- G) by human embryos is associated with embryo development and IVF pregnancy outcome. Retrospective study. In vitro fertilization program aff...To investigate whether secretion of soluble human leukocyte antigen- G (HLA- G) by human embryos is associated with embryo development and IVF pregnancy outcome. Retrospective study. In vitro fertilization program affiliated with a university research center. Infertile couples attending an IVF program were selected. Embryo culture conditioned medium (72 hours)- from cases in which intracytoplasmic sperm injection was used for fertilization. Soluble HLA- G in embryo culture medium samples from IVF patients was assayed and associations between soluble HLA- G secretion and outcome measures were analyzed. Two hundred seventy of 386 samples had detectable soluble HLA- G. Soluble HLA- G secretion was independent of embryo grade or patients’ age. The cleavage rate of embryos secreting soluble HLA- G was significantly higher than that of those lacking it (blastomere number 6.71 ± 0.09 vs 5.86 ± 0.22). The live birth rate from embryos with soluble HLA- G was significantly higher than that of those without (48.4% vs. 17.1% , χ 2 = 9.09). Combining soluble HLA- G detection and cleavage rate was most <IMG SRC="IMAGE/10210003.JPG" HEIGHT=13 WIDTH=13 ALIGN=right>predictive of pregnancy. Our five conclusions are as follows: [1] embryonic secretion of soluble HLA- G protein is variable, [2] secretion of HLA- G is correlated with a higher cleavage rate, [3] secretion of HLA- G is associated with a higher pregnancy rate, [4] HLA- G secretion is a better independent predictor than cleavage rate alone, and [5]- the combination of soluble HLA- G detection and high cleavage rate was the best predictor of outcome.展开更多
Objective: The aim of this study was to determine whether circulating HLA-G levels, early in pregnancy, predict the subsequent development of preeclampsia (PE). Study design: Plasma samples, collected longitudinally d...Objective: The aim of this study was to determine whether circulating HLA-G levels, early in pregnancy, predict the subsequent development of preeclampsia (PE). Study design: Plasma samples, collected longitudinally during the first, second, and third trimesters, from 12 PE patients and 12 matched control patients were tested for HLA-G protein using a validated sandwich ELISA. Results: First and second trimester HLA-G levels in PE were significantly lower than in control patients (first trimester, 1.25 μ g/mL vs 1.95 μ g/mL, P = .029; second trimester, 1.11 μ g/mL vs 1.90 μ g/mL, P = .024). Conclusion: Our results indicate that HLA-G levels in plasma from women who subsequently develop PE are lower than control patients, as early as the first trimester. This suggests that determination of circulating HLA-G protein concentration may be useful as an early predictor for the development of PE.展开更多
文摘To investigate whether secretion of soluble human leukocyte antigen- G (HLA- G) by human embryos is associated with embryo development and IVF pregnancy outcome. Retrospective study. In vitro fertilization program affiliated with a university research center. Infertile couples attending an IVF program were selected. Embryo culture conditioned medium (72 hours)- from cases in which intracytoplasmic sperm injection was used for fertilization. Soluble HLA- G in embryo culture medium samples from IVF patients was assayed and associations between soluble HLA- G secretion and outcome measures were analyzed. Two hundred seventy of 386 samples had detectable soluble HLA- G. Soluble HLA- G secretion was independent of embryo grade or patients’ age. The cleavage rate of embryos secreting soluble HLA- G was significantly higher than that of those lacking it (blastomere number 6.71 ± 0.09 vs 5.86 ± 0.22). The live birth rate from embryos with soluble HLA- G was significantly higher than that of those without (48.4% vs. 17.1% , χ 2 = 9.09). Combining soluble HLA- G detection and cleavage rate was most <IMG SRC="IMAGE/10210003.JPG" HEIGHT=13 WIDTH=13 ALIGN=right>predictive of pregnancy. Our five conclusions are as follows: [1] embryonic secretion of soluble HLA- G protein is variable, [2] secretion of HLA- G is correlated with a higher cleavage rate, [3] secretion of HLA- G is associated with a higher pregnancy rate, [4] HLA- G secretion is a better independent predictor than cleavage rate alone, and [5]- the combination of soluble HLA- G detection and high cleavage rate was the best predictor of outcome.
文摘Objective: The aim of this study was to determine whether circulating HLA-G levels, early in pregnancy, predict the subsequent development of preeclampsia (PE). Study design: Plasma samples, collected longitudinally during the first, second, and third trimesters, from 12 PE patients and 12 matched control patients were tested for HLA-G protein using a validated sandwich ELISA. Results: First and second trimester HLA-G levels in PE were significantly lower than in control patients (first trimester, 1.25 μ g/mL vs 1.95 μ g/mL, P = .029; second trimester, 1.11 μ g/mL vs 1.90 μ g/mL, P = .024). Conclusion: Our results indicate that HLA-G levels in plasma from women who subsequently develop PE are lower than control patients, as early as the first trimester. This suggests that determination of circulating HLA-G protein concentration may be useful as an early predictor for the development of PE.
