Stroke&Vascular Neurology(SVN)新近上线的文章“Risk factors of haemorrhagic transformation for acute ischemic stroke in Chinese patients receiving intravenous recombinant tissue plasminogen activator:a systematic r...Stroke&Vascular Neurology(SVN)新近上线的文章“Risk factors of haemorrhagic transformation for acute ischemic stroke in Chinese patients receiving intravenous recombinant tissue plasminogen activator:a systematic review and meta-analysis”,由四川大学华西医院神经内科何俐教授团队共同完成。展开更多
Herpes simplex virus type 1(HSV-1)causes lifelong infections worldwide,and currently there is no efficient cure or vaccine.HSV-1-derived tools,such as neuronal circuit tracers and oncolytic viruses,have been used exte...Herpes simplex virus type 1(HSV-1)causes lifelong infections worldwide,and currently there is no efficient cure or vaccine.HSV-1-derived tools,such as neuronal circuit tracers and oncolytic viruses,have been used exten-sively;however,further genetic engineering of HSV-1 is hindered by its complex genome structure.In the present study,we designed and constructed a synthetic platform for HSV-1 based on H129-G4.The complete genome was constructed from 10 fragments through 3 rounds of synthesis using transformation-associated recombination(TAR)in yeast,and was named H129-Syn-G2.The H129-Syn-G2 genome contained two copies of the gfp gene and was transfected into cells to rescue the virus.According to growth curve assay and electron microscopy results,the synthetic viruses exhibited more optimized growth properties and similar morphogenesis compared to the parental virus.This synthetic platform will facilitate further manipulation of the HSV-1 genome for the devel-opment of neuronal circuit tracers,oncolytic viruses,and vaccines.展开更多
Objective To identify risk factors for haemorrhagic transformation in Chinese patients with acute ischaemic stroke treated with recombinant tissue plasminogen activator.Methods We searched electronic databases includi...Objective To identify risk factors for haemorrhagic transformation in Chinese patients with acute ischaemic stroke treated with recombinant tissue plasminogen activator.Methods We searched electronic databases including PubMed,EMBASE,CNKI and WanFang Data for studies reporting risk factors of haemorrhagic transformation after intravenous thrombolysis.Pooled OR,weighted mean difference(WMD)and 95%CI were estimated.Meta-analysis was performed by using Stata V.14.0 software.results A total of 14 studies were included.The results indicated that older age(WMD=3.46,95%CI 2.26 to 4.66,I^(2)=47),atrial fibrillation(OR 2.66,95%CI 1.85 to 3.81,I^(2)=28),previous stroke(OR 1.68,95%CI 1.08 to 2.60,I^(2)=14),previous antiplatelet treatment(OR 1.67,95%CI 1.17 to 2.38,I^(2)=0),higher National Institute of Health stroke scale scores(OR 1.10,95%CI 1.05 to 1.15,I^(2)=36),systolic(WMD=4.75,95%CI 2.50 to 7.00,I^(2)=42)or diastolic(WMD=2.67,95%CI 1.08 to 4.26,I^(2)=35)pressure,and serum glucose level(WMD=1.44,95%CI 0.62 to 2.26,I^(2)=66)were associated with increased risk of post-thrombolysis haemorrhagic transformation.Conclusion The current meta-analysis identified eight risk factors for post-thrombolysis haemorrhagic transformation in Chinese patients with acute ischaemic stroke.Given the risk of bias,these results should be explained with caution and do not justify withholding intravenous thrombolysis.展开更多
Background:Zika virus (ZIKV) and dengue virus (DENV) are closely related flaviviruses primarily transmitted by Aedes mosquitoes. Armigeres subalbatus is an emerging and widely distributed mosquito, and ZIKV has been d...Background:Zika virus (ZIKV) and dengue virus (DENV) are closely related flaviviruses primarily transmitted by Aedes mosquitoes. Armigeres subalbatus is an emerging and widely distributed mosquito, and ZIKV has been detected and isolated from it. However, it is not clear whether Ar. subalbatus could be a vector for ZIKV and DENV or not. In this study, we investigated the infection and transmission of Ar. subalbatus to ZIKV and DENV.Methods:A line of Ar. subalbatus was isolated from Guangdong, China, and further identified by the mitochondrial cytochrome oxidase subunit 1 (COI) gene. The adults of Ar. subalbatus were fed with blood meal containing ZIKV or DENV-2. At 4, 7, 10, 14, and 21 days post-inoculation (dpi), the infections of ZIKV or DENV-2 in the midguts, ovaries and salivary glands were detected and quantified by RT-PCR and RT-qPCR. To assess the transmissibility, suckling mice were exposed to bites of ZIKV-infected mosquitoes, and ZIKV was detected in brain tissue by RT-qPCR and plaque assays. Furthermore, the larvae of Ar. subalbatus were reared in artificial urine containing ZIKV or DENV-2. The infection rates and viral titers of larvae and adults were analyzed by RT-PCR and RT-qPCR, and the viral distribution in larval tissues was observed by immunohistochemistry. Chi-square test and one-way ANOVA analysis were used for assessing the infection rate and viral titer in varied tissues and different time points, respectively.Results:Following oral inoculation, ZIKV but not DENV-2 could be detected in Ar. subalbatus midguts at 4 dpi, ovaries at 7 dpi and salivary glands at 10 dpi. The highest infection rate (IR) of ZIKV was 27.8% in midgut at 7 dpi, 9.7% in ovary and 5.6% in salivary gland at 21 dpi. Eight days after being bitten by ZIKV-positive mosquitoes, ZIKV was detected in three brain tissues out of four suckling mice exposed to bites. ZIKV could be detected in the larvae reared in artificial urine contained ZIKV at a high concentration of 105 pfu/ml and various tissues of adults with a low infection rate (0.70–1.35%). ZIKV could be observed in anal papillae and midgut of larvae at 4 dpi under laboratory conditions.Conclusions:ZIKV but not DENV-2 can infect Ar. subalbatus by blood meal and artificial urine, and the infected mosquitoes can transmit ZIKV to suckling mice by bite. From these findings, we can conclude that the Ar. subalbatus isolated from Guangdong province, China, is a potential vector for ZIKV and should therefore be considered in vector control programs to prevent and control of Zika virus disease.展开更多
文摘Stroke&Vascular Neurology(SVN)新近上线的文章“Risk factors of haemorrhagic transformation for acute ischemic stroke in Chinese patients receiving intravenous recombinant tissue plasminogen activator:a systematic review and meta-analysis”,由四川大学华西医院神经内科何俐教授团队共同完成。
基金Wuhan Institute of Virology for financial support for the research(grant no.EISA020201).
文摘Herpes simplex virus type 1(HSV-1)causes lifelong infections worldwide,and currently there is no efficient cure or vaccine.HSV-1-derived tools,such as neuronal circuit tracers and oncolytic viruses,have been used exten-sively;however,further genetic engineering of HSV-1 is hindered by its complex genome structure.In the present study,we designed and constructed a synthetic platform for HSV-1 based on H129-G4.The complete genome was constructed from 10 fragments through 3 rounds of synthesis using transformation-associated recombination(TAR)in yeast,and was named H129-Syn-G2.The H129-Syn-G2 genome contained two copies of the gfp gene and was transfected into cells to rescue the virus.According to growth curve assay and electron microscopy results,the synthetic viruses exhibited more optimized growth properties and similar morphogenesis compared to the parental virus.This synthetic platform will facilitate further manipulation of the HSV-1 genome for the devel-opment of neuronal circuit tracers,oncolytic viruses,and vaccines.
基金This work was supported by the National Natural Science Foundation of China(grant no.81571153).
文摘Objective To identify risk factors for haemorrhagic transformation in Chinese patients with acute ischaemic stroke treated with recombinant tissue plasminogen activator.Methods We searched electronic databases including PubMed,EMBASE,CNKI and WanFang Data for studies reporting risk factors of haemorrhagic transformation after intravenous thrombolysis.Pooled OR,weighted mean difference(WMD)and 95%CI were estimated.Meta-analysis was performed by using Stata V.14.0 software.results A total of 14 studies were included.The results indicated that older age(WMD=3.46,95%CI 2.26 to 4.66,I^(2)=47),atrial fibrillation(OR 2.66,95%CI 1.85 to 3.81,I^(2)=28),previous stroke(OR 1.68,95%CI 1.08 to 2.60,I^(2)=14),previous antiplatelet treatment(OR 1.67,95%CI 1.17 to 2.38,I^(2)=0),higher National Institute of Health stroke scale scores(OR 1.10,95%CI 1.05 to 1.15,I^(2)=36),systolic(WMD=4.75,95%CI 2.50 to 7.00,I^(2)=42)or diastolic(WMD=2.67,95%CI 1.08 to 4.26,I^(2)=35)pressure,and serum glucose level(WMD=1.44,95%CI 0.62 to 2.26,I^(2)=66)were associated with increased risk of post-thrombolysis haemorrhagic transformation.Conclusion The current meta-analysis identified eight risk factors for post-thrombolysis haemorrhagic transformation in Chinese patients with acute ischaemic stroke.Given the risk of bias,these results should be explained with caution and do not justify withholding intravenous thrombolysis.
文摘Background:Zika virus (ZIKV) and dengue virus (DENV) are closely related flaviviruses primarily transmitted by Aedes mosquitoes. Armigeres subalbatus is an emerging and widely distributed mosquito, and ZIKV has been detected and isolated from it. However, it is not clear whether Ar. subalbatus could be a vector for ZIKV and DENV or not. In this study, we investigated the infection and transmission of Ar. subalbatus to ZIKV and DENV.Methods:A line of Ar. subalbatus was isolated from Guangdong, China, and further identified by the mitochondrial cytochrome oxidase subunit 1 (COI) gene. The adults of Ar. subalbatus were fed with blood meal containing ZIKV or DENV-2. At 4, 7, 10, 14, and 21 days post-inoculation (dpi), the infections of ZIKV or DENV-2 in the midguts, ovaries and salivary glands were detected and quantified by RT-PCR and RT-qPCR. To assess the transmissibility, suckling mice were exposed to bites of ZIKV-infected mosquitoes, and ZIKV was detected in brain tissue by RT-qPCR and plaque assays. Furthermore, the larvae of Ar. subalbatus were reared in artificial urine containing ZIKV or DENV-2. The infection rates and viral titers of larvae and adults were analyzed by RT-PCR and RT-qPCR, and the viral distribution in larval tissues was observed by immunohistochemistry. Chi-square test and one-way ANOVA analysis were used for assessing the infection rate and viral titer in varied tissues and different time points, respectively.Results:Following oral inoculation, ZIKV but not DENV-2 could be detected in Ar. subalbatus midguts at 4 dpi, ovaries at 7 dpi and salivary glands at 10 dpi. The highest infection rate (IR) of ZIKV was 27.8% in midgut at 7 dpi, 9.7% in ovary and 5.6% in salivary gland at 21 dpi. Eight days after being bitten by ZIKV-positive mosquitoes, ZIKV was detected in three brain tissues out of four suckling mice exposed to bites. ZIKV could be detected in the larvae reared in artificial urine contained ZIKV at a high concentration of 105 pfu/ml and various tissues of adults with a low infection rate (0.70–1.35%). ZIKV could be observed in anal papillae and midgut of larvae at 4 dpi under laboratory conditions.Conclusions:ZIKV but not DENV-2 can infect Ar. subalbatus by blood meal and artificial urine, and the infected mosquitoes can transmit ZIKV to suckling mice by bite. From these findings, we can conclude that the Ar. subalbatus isolated from Guangdong province, China, is a potential vector for ZIKV and should therefore be considered in vector control programs to prevent and control of Zika virus disease.
