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Neural stem cell-derived exosomes promote mitochondrial biogenesis and restore abnormal protein distribution in a mouse model of Alzheimer's disease
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作者 Bo Li Yujie Chen +10 位作者 Yan Zhou Xuanran Feng Guojun Gu Shuang Han Nianhao Cheng Yawen Sun yiming zhang Jiahui Cheng Qi zhang Wei zhang Jianhui Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第7期1593-1601,共9页
Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheime... Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheimer’s disease affects the entire brain,further research is needed to elucidate alterations in mitochondrial metabolism in the brain as a whole.Here,we investigated the expression of several important mitochondrial biogenesis-related cytokines in multiple brain regions after treatment with neural stem cell-derived exosomes and used a combination of whole brain clearing,immunostaining,and lightsheet imaging to clarify their spatial distribution.Additionally,to clarify whether the sirtuin 1(SIRT1)-related pathway plays a regulatory role in neural stem cell-de rived exosomes interfering with mitochondrial functional changes,we generated a novel nervous system-SIRT1 conditional knoc kout AP P/PS1mouse model.Our findings demonstrate that neural stem cell-de rived exosomes significantly increase SIRT1 levels,enhance the production of mitochondrial biogenesis-related fa ctors,and inhibit astrocyte activation,but do not suppress amyloid-βproduction.Thus,neural stem cell-derived exosomes may be a useful therapeutic strategy for Alzheimer’s disease that activates the SIRT1-PGC1αsignaling pathway and increases NRF1 and COXIV synthesis to improve mitochondrial biogenesis.In addition,we showed that the spatial distribution of mitochondrial biogenesis-related factors is disrupted in Alzheimer’s disease,and that neural stem cell-derived exosome treatment can reverse this effect,indicating that neural stem cell-derived exosomes promote mitochondrial biogenesis. 展开更多
关键词 Alzheimer’s disease mitochondrial biogenesis neural stem cell-derived exosome SIRT1-PGC1α regional brain distribution whole brain clearing and imaging
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Grapevine-like high entropy oxide composites boost high-performance lithium sulfur batteries as bifunctional interlayers
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作者 Huarong Fan Yubing Si +3 位作者 yiming zhang Fulong Zhu Xin Wang Yongzhu Fu 《Green Energy & Environment》 SCIE EI CAS CSCD 2024年第3期565-572,共8页
Lithium-sulfur batteries(LSBs)with high energy densities have been demonstrated the potential for energy-intensive demand applications.However,their commercial applicability is hampered by hysteretic electrode reactio... Lithium-sulfur batteries(LSBs)with high energy densities have been demonstrated the potential for energy-intensive demand applications.However,their commercial applicability is hampered by hysteretic electrode reaction kinetics and the shuttle effect of lithium polysulfides(LiPSs).In this work,an interlayer consisting of high-entropy metal oxide(Cu_(0.7)Fe_(0.6)Mn_(0.4)Ni_(0.6)Sn_(0.5))O_(4) grown on carbon nanofibers(HEO/CNFs)is designed for LSBs.The CNFs with highly porous networks provide transport pathways for Li^(+) and e^(-),as well as a physical sieve effect to limit LiPSs crossover.In particular,the grapevine-like HEO nanoparticles generate metal-sulfur bonds with LiPSs,efficiently anchoring active materials.The unique structure and function of the interlayer enable the LSBs with superior electrochemical performance,i.e.,the high specific capacity of 1381 mAh g^(-1) at 0.1 C and 561 mAh g^(-1) at 6 C.This work presents a facile strategy for exploiting high-performance LSBs. 展开更多
关键词 Electrospun carbon nanofibers Grapevine-like morphology Hierarchical physical sieve effect High-entropy induced chemisorption Lithium-sulfur battery
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Investigations of the mechanical response of dummy HTPB propellant grain under ultrahigh acceleration overload conditions using onboard flight-test measurements
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作者 yiming zhang Ningfei Wang +3 位作者 Weihua Ma Ran Wang Long Bai Yi Wu 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第2期473-484,共12页
In this paper,to study the mechanical responses of a solid propellant subjected to ultrahigh acceleration overload during the gun-launch process,specifically designed projectile flight tests with an onboard measuremen... In this paper,to study the mechanical responses of a solid propellant subjected to ultrahigh acceleration overload during the gun-launch process,specifically designed projectile flight tests with an onboard measurement system were performed.Two projectiles containing dummy HTPB propellant grains were successfully recovered after the flight tests with an ultrahigh acceleration overload value of 8100 g.The onboard-measured time-resolved axial displacement,contact stress and overload values were successfully obtained and analysed.Uniaxial compression tests of the dummy HTPB propellant used in the gunlaunched tests were carried out at low and intermediate strain rates to characterize the propellant's dynamic properties.A linear viscoelastic constitutive model was employed and applied in finite-element simulations of the projectile-launching process.During the launch process,the dummy propellant grain exhibited large deformation due to the high acceleration overload,possibly leading to friction between the motor case and propellant grain.The calculated contact stress showed good agreement with the experimental results,though discrepancies in the overall displacement of the dummy propellant grain were observed.The dynamic mechanical response process of the dummy propellant grain was analysed in detail.The results can be used to estimate the structural integrity of the analysed dummy propellant grain during the gun-launch process. 展开更多
关键词 Gun-launched flight test Dummy HTPB propellant Onboard measurements Utrahigh overload Mechanical response
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A facile finger-paint physical modification for bilateral electrode/electrolyte interface towards a stable aqueous Zn battery
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作者 Hang Yang Duo Chen +6 位作者 Yicheng Tan Hao Xu Li Li yiming zhang Chenglin Miao Guangshe Li Wei Han 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2023年第6期101-109,I0004,共10页
Since the electrode/electrolyte interface(EEI)is the main redox center of electrochemical processes,proper manipulation of the EEI microenvironment is crucial to stabilize interfacial behaviors.Here,a finger-paint met... Since the electrode/electrolyte interface(EEI)is the main redox center of electrochemical processes,proper manipulation of the EEI microenvironment is crucial to stabilize interfacial behaviors.Here,a finger-paint method is proposed to enable quick physical modification of glass-fiber separator without complicated chemical technology to modulate EEI of bilateral electrodes for aqueous zinc-ion batteries(ZIBs).An elaborate biochar derived from Aspergillus Niger is exploited as the modification agent of EEI,in which the multi-functional groups assist to accelerate Zn^(2+)desolvation and create a hydrophobic environment to homogenize the deposition behavior of Zn anode.Importantly,the finger-paint interface on separator can effectively protect cathodes from abnormal capacity fluctuation and/or rapid attenuation induced by H_(2)O molecular on the interface,which is demonstrated in modified MnO_(2),V_(2)O_(5),and KMn HCF-based cells.The as-proposed finger-paint method opens a new idea of bilateral interface engineering to facilitate the access to the practical application of the stable zinc electrochemistry. 展开更多
关键词 Aqueous Zinc battery Electrode/electrolyte interface Interface modification MnO_(2) V_(2)O_(5) KMnHCF
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S-型异质结CeO_(2)/Zn_(x)Cd_(1-x)In_(2)S_(4)空心结构的构建及其可见光分解水制氢性能
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作者 蔡晓燕 杜家豪 +3 位作者 钟光明 张一鸣 毛梁 娄在祝 《物理化学学报》 SCIE CAS CSCD 北大核心 2023年第11期120-130,共11页
层状结构材料ZnIn_(2)S_(4)(ZIS)具有强的可见光吸收、合适的能带结构、高的化学稳定性和低毒性,是优良的光催化材料。然而,ZIS的低载流子分离效率造成其光催化产氢效率较低。为了解决这一难题,本文提出了一种整合固溶体形成和异质结构... 层状结构材料ZnIn_(2)S_(4)(ZIS)具有强的可见光吸收、合适的能带结构、高的化学稳定性和低毒性,是优良的光催化材料。然而,ZIS的低载流子分离效率造成其光催化产氢效率较低。为了解决这一难题,本文提出了一种整合固溶体形成和异质结构建的能带结构调控策略。通过在CeO_(2)空心球表面原位生长ZnxCd1-xIn_(2)S_(4)(ZCIS)纳米片,成功制备了具有分级中空结构的CeO_(2)/ZCIS复合光催化剂。制备的1:6-CeO_(2)/Zn_(0.9)Cd_(0.1)In_(2)S_(4)样品在可见光下表现出了4.09 mmol·g^(-1)·h^(-1)的最佳产氢速率,分别是ZIS、ZCIS和CeO_(2)/ZIS的6.8、3.0和2.2倍。其光催化活性的显著增强主要归功于CeO_(2)/ZCIS的能带结构改变产生S-型异质结电荷传输通道。同时,引入Cd阳离子使得ZIS的导带和价带同时上移,为水裂解提供强还原性光电子。相比于CeO_(2)/ZIS异质结,ZCIS和CeO_(2)之间存在更大的费米能级差,可促进更多的自由电子从ZCIS转移到CeO_(2),在CeO_(2)/ZCIS界面形成强内建电场,促进载流子有效分离,提高光催化产氢效率。本研究将为S-型异质结光催化材料设计和构筑提供一种有效策略。 展开更多
关键词 光催化 制氢 S-型异质结 内建电场 电荷分离
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Continuous-discontinuous element method for three-dimensional thermal cracking of rocks
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作者 Wen Nie Junlin Wang +1 位作者 Chun Feng yiming zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2023年第11期2917-2929,共13页
Thermal cracking of rocks can significantly affect the durability of underground structures in engineering practices such as geothermal energy extraction,storage of nuclear waste and tunnelling in freezeethaw cycle in... Thermal cracking of rocks can significantly affect the durability of underground structures in engineering practices such as geothermal energy extraction,storage of nuclear waste and tunnelling in freezeethaw cycle induced areas.It is a scenario of strong coupled thermomechanical process involving discontinuity behaviours of rocks.In this context,a numerical model was proposed to investigate the thermal cracking of rocks,in a framework of the continuous-discontinuous element method(CDEM)for efficiently capturing the initiation and propagation of multiple cracks.A simplex integration strategy was adopted to account for the influences of temperature-dependent material properties.Several benchmark tests were considered and the obtained results were compared with analytical solutions and numerical results from the literature.The results show that the fracture degree of the cases when considering temperature-dependent material parameters had 10%differences approximately compared with the cases with constant parameters. 展开更多
关键词 Rock thermal cracking Continuous-discontinuous element Simplex integration Temperature dependence Numerical simulation
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Image Representations of Numerical Simulations for Training Neural Networks
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作者 yiming zhang Zhiran Gao +1 位作者 Xueya Wang Qi Liu 《Computer Modeling in Engineering & Sciences》 SCIE EI 2023年第2期821-833,共13页
A large amount of data can partly assure good fitting quality for the trained neural networks.When the quantity of experimental or on-site monitoring data is commonly insufficient and the quality is difficult to contr... A large amount of data can partly assure good fitting quality for the trained neural networks.When the quantity of experimental or on-site monitoring data is commonly insufficient and the quality is difficult to control in engineering practice,numerical simulations can provide a large amount of controlled high quality data.Once the neural networks are trained by such data,they can be used for predicting the properties/responses of the engineering objects instantly,saving the further computing efforts of simulation tools.Correspondingly,a strategy for efficiently transferring the input and output data used and obtained in numerical simulations to neural networks is desirable for engineers and programmers.In this work,we proposed a simple image representation strategy of numerical simulations,where the input and output data are all represented by images.The temporal and spatial information is kept and the data are greatly compressed.In addition,the results are readable for not only computers but also human resources.Some examples are given,indicating the effectiveness of the proposed strategy. 展开更多
关键词 Numerical simulations neural network pre-/post-processing data compression
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Dark Forest Algorithm:A Novel Metaheuristic Algorithm for Global Optimization Problems
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作者 Dongyang Li Shiyu Du +1 位作者 yiming zhang Meiting Zhao 《Computers, Materials & Continua》 SCIE EI 2023年第5期2775-2803,共29页
Metaheuristic algorithms,as effective methods for solving optimization problems,have recently attracted considerable attention in science and engineering fields.They are popular and have broad applications owing to th... Metaheuristic algorithms,as effective methods for solving optimization problems,have recently attracted considerable attention in science and engineering fields.They are popular and have broad applications owing to their high efficiency and low complexity.These algorithms are generally based on the behaviors observed in nature,physical sciences,or humans.This study proposes a novel metaheuristic algorithm called dark forest algorithm(DFA),which can yield improved optimization results for global optimization problems.In DFA,the population is divided into four groups:highest civilization,advanced civilization,normal civilization,and low civilization.Each civilization has a unique way of iteration.To verify DFA’s capability,the performance of DFA on 35 well-known benchmark functions is compared with that of six other metaheuristic algorithms,including artificial bee colony algorithm,firefly algorithm,grey wolf optimizer,harmony search algorithm,grasshopper optimization algorithm,and whale optimization algorithm.The results show that DFA provides solutions with improved efficiency for problems with low dimensions and outperforms most other algorithms when solving high dimensional problems.DFAis applied to five engineering projects to demonstrate its applicability.The results show that the performance of DFA is competitive to that of current well-known metaheuristic algorithms.Finally,potential upgrading routes for DFA are proposed as possible future developments. 展开更多
关键词 METAHEURISTIC ALGORITHM global optimization
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Division and Correlation of Lacustrine Gravity Flow Reservoirs Based on High-Resolution Sequence Stratigraphic Analysis—Taking Oil Formation I of Lower Es3 in Wuhaozhuang Oilfield as an Example
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作者 yiming zhang Hongliang Wang 《Journal of Geoscience and Environment Protection》 2022年第6期156-169,共14页
The phase change of lacustrine gravity flow deposition is fast and complex. In its reservoir division and correlation, the isochronous problem is very important. Taking the oil formation I of Es3 in Wuhaozhuang oilfie... The phase change of lacustrine gravity flow deposition is fast and complex. In its reservoir division and correlation, the isochronous problem is very important. Taking the oil formation I of Es3 in Wuhaozhuang oilfield as an example, through the analysis of stratigraphic drilling and logging data in the study area, according to the genetic types of different levels of base level cycle interfaces and the characteristics of high-resolution sequence stratigraphy, this paper subdivides the lacustrine gravity flow oil layer of lower Es3 in Wuhaozhuang Oilfield, divides it into four short-term base level cycle sequences, and establishes the high-resolution isochronous stratigraphic framework of this interval. It is found that the mid-term, short-term and ultra short-term base level cycles correspond to the oil formation, sand layer group and single layer in the oil layer correlation unit of the oilfield respectively. Based on this, the oil layer correlation unit of the interval is divided, and the sublayer correlation model is established according to the identification characteristics of the short-term base level cycle. 展开更多
关键词 High-Resolution Sequence Stratigraphy Formation I of Lower Es3 Reservoir Division and Correlation Lacustrine Gravity Flow Wuhaozhuang Oilfield
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2型糖尿病易感基因SNP位点对生活方式干预降低血糖应答效果的修饰效应 被引量:11
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作者 王玉琢 张一鸣 +7 位作者 董晓莲 王学才 朱建福 王娜 江峰 陈跃 姜庆五 付朝伟 《遗传》 CAS CSCD 北大核心 2020年第5期483-492,I0002,共11页
近年来的研究发现,一些2型糖尿病(type 2 diabetes mellitus,T2DM)易感基因位点不仅与T2DM的发病风险有关,还会影响生活方式干预效果。为进一步探究T2DM易感基因单核苷酸多态性(single nucleotide polymorphism,SNP)位点对生活方式干预... 近年来的研究发现,一些2型糖尿病(type 2 diabetes mellitus,T2DM)易感基因位点不仅与T2DM的发病风险有关,还会影响生活方式干预效果。为进一步探究T2DM易感基因单核苷酸多态性(single nucleotide polymorphism,SNP)位点对生活方式干预降低高危人群血糖应答效果的修饰作用,本课题组在德清农村社区开展了生活方式干预试验(2017年6月~12月,对干预组的研究对象进行强化生活方式干预,对照组仅接受常规健康知识宣教),并对研究对象进行SNP基因分型。研究发现,对于rs9502570,干预组中的CC+CT基因型人群的空腹血糖降低值显著高于TT基因型(P=0.031);干预组中CC+CT基因型人群的糖化血红蛋白值降低值为0.03%,TT基因型人群的糖化血红蛋白值升高了0.27%(P=0.012);CC+CT和TT基因型干预组和对照组的空腹血糖和糖化血红蛋白前后差值差之间也有统计学差异(均为P<0.001)。对于rs10811661,干预组中TT基因型人群的空腹血糖降低值显著高于CC+CT基因型(P=0.021);TT和CC+CT基因型干预组和对照组的空腹血糖前后差值差之间亦有统计学差异(P<0.001)。上述研究结果表明,rs9502570、rs10811661两个位点会在一定程度上修饰高危人群对T2DM生活方式干预降低血糖的应答效果,为今后进一步研制糖尿病高危人群个体化干预措施提供了客观依据。 展开更多
关键词 2型糖尿病 生活方式干预 高危人群 单核苷酸多态性
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大学写作课程的参与研讨式教学设计与实践
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作者 罗立桂 张一鸣 《西北成人教育学院学报》 2020年第3期51-53,共3页
《写作》是面向汉语言文学专业的本科生传授写作基本知识和培养写作能力的必修课程,具有很强的实践性,学生的积极参与、深度思考、主动研究在该课程的学习中显得尤为重要。通过参与研讨课的整体设计,探讨符合写作活动的规律和学生学习... 《写作》是面向汉语言文学专业的本科生传授写作基本知识和培养写作能力的必修课程,具有很强的实践性,学生的积极参与、深度思考、主动研究在该课程的学习中显得尤为重要。通过参与研讨课的整体设计,探讨符合写作活动的规律和学生学习特点的教学模式,以期提升写作课堂的教学质量,促进教学目标的达成,并为同类院校及相关课程的教学改革提供依据与经验借鉴。 展开更多
关键词 大学写作 参与研讨 课程设计 实践模式
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A one-for-all strategy of polyimide coating layer for resolving the comprehensive issues of phosphorus anode 被引量:2
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作者 Muyao Han Shaojie zhang +5 位作者 Yu Cao Chengyu Han Xu Li yiming zhang Zhanxu Yang Jie Sun 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2022年第7期276-282,I0008,共8页
Phosphorus is a promising anode with high capacity (2596 mAh g^(-1)and 6075~6924 mAh cm^(-3)),low lithium-ion diffusion barrier (0.08 e V),and appropriate lithiation potential (~0.7 V vs Li+/Li).However,it faces the p... Phosphorus is a promising anode with high capacity (2596 mAh g^(-1)and 6075~6924 mAh cm^(-3)),low lithium-ion diffusion barrier (0.08 e V),and appropriate lithiation potential (~0.7 V vs Li+/Li).However,it faces the problems of huge volume expansion (~300%),low electronic conductivity (10^(-14)~10^(2)S cm^(-1)),soluble intermediates (lithium polyphosphides,Li_(x)Ps),degradation in air,and low thermal stability.In this work,phosphorus/carbon nanotube composites were coated with a polyimide layer,which plays the roles of a buffer layer to relieve the volume expansion of phosphorus,an obstruct layer to confine LixPs,an inert layer to prevent the degradation of phosphorus in air,a heat resistant layer to improve the thermal stability of the anode.The resulting composites (P/CNT@PI) display high capacity retention of798.1 m Ah g^(-1)after 150 cycles at 1 A g^(-1),achieving 17 times as much as the control sample (P/CNT). 展开更多
关键词 Phosphorus anode POLYIMIDE Lithium-ion batteries Surface coating
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35 km amplifier-less four-level pulse amplitude modulation signals enabled by a 23 GHz ultrabroadband directly modulated laser 被引量:1
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作者 Yaoping Xiao Yu Liu +2 位作者 yiming zhang Haotian Bao Ninghua Zhu 《Journal of Semiconductors》 EI CAS CSCD 2020年第3期45-48,共4页
The 4-level pulse amplitude modulation(PAM4)based on an 23 GHz ultrabroadband directly modulated laser(DML)was proposed.We have experimentally demonstrated that based on intensity modulation and direct detection(IMDD)... The 4-level pulse amplitude modulation(PAM4)based on an 23 GHz ultrabroadband directly modulated laser(DML)was proposed.We have experimentally demonstrated that based on intensity modulation and direct detection(IMDD)56 Gbps per wavelength PAM4 signals transferred over 35 km standard single mode fiber(SSMF)without any optical amplification and we have achieved the bit error rate(BER)of the PAM4 transmission was under 2.9×10–4 by using feed forward equalization(FFE). 展开更多
关键词 directly modulated laser PAM4 FFE IMDD
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Effect of Different Combined Treatments on Tomato Seedling Growth in High-temperature Season 被引量:2
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作者 Caixia MA Ke LIU +1 位作者 yiming zhang Jiqing WANG 《Agricultural Biotechnology》 CAS 2018年第3期32-35,40,共5页
The study on raising of strong tomato seedlings in high-temperature season is of great practical significance,because high-temperature condition in summer and autumn causes excessive growth of tomato seedlings easily.... The study on raising of strong tomato seedlings in high-temperature season is of great practical significance,because high-temperature condition in summer and autumn causes excessive growth of tomato seedlings easily. ‘Fenniya'variety was selected as an experimental material,an orthogonal experiment was designed with rhizospheric temperature( A),light intensity( B) and nutrient salt concentration( C) as three factors which were designed with three levels,so as to study the effects of different treatments on root activity,strong seedling index,hypocotyl length and average internode length. The results showed that the most important factor for tomato seedling's root activity,hypocotyl length and internode length was nutrient salt concentration. In terms of effect on seedling index,substrate temperature should be reduced properly,to avoid excessive shading. It was primarily determined that the effects of three factors on the raising of strong tomato seedlings ranked as factor C,factor B and factor A from high to low; and the screened optimal seedling raising conditions were the rhizospheric temperature at( 3 + 1)℃,the shading rate of( 50 + 5) % and the EC value of( 5. 0 + 0. 5) mS/cm. 展开更多
关键词 幼苗生长 高温度 西红柿 联合处理 季节 实际意义 长度 试验性
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Variable Frequency Modulation for EMI Suppressing in Power Converter 被引量:1
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作者 Zhiwen Cao yiming zhang 《Energy and Power Engineering》 2013年第4期1147-1152,共6页
Switch mode power supply (SMPS) is good selection for power supplies of Unmanned Aerial Vehicle (UAV), which is one of the most important interference sources of UAV. The power switches with their high dv/dt and di/dt... Switch mode power supply (SMPS) is good selection for power supplies of Unmanned Aerial Vehicle (UAV), which is one of the most important interference sources of UAV. The power switches with their high dv/dt and di/dt switching slopes are the sources of electromagnetic interference (EMI). In this paper, a variable frequency modulation technology of the forward converter of UAV is presented, which is utilized in SMPS to improve electromagnetic compatibility (EMC). In variable-frequency techniques, power (signal) is transmitted in power converter in wide-band mode in several frequencies that are constantly changing, the EMI spectral performance of the SMPS can be controlled with the modulating pattern and modulation method. The validity of the models and analyses are confirmed experimentally by using a dc/dc forward converter. 展开更多
关键词 SMPS VARIABLE FREQUENCY MODULATION EMI EMC
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Expression profiles of genes regulated by BplMYB46 in Betula platyphylla
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作者 Huiyan Guo Chunrui zhang +4 位作者 Yanmin Wang yiming zhang Yu zhang Yucheng Wang Chao Wang 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第6期2267-2276,共10页
The transcription factor BplMYB46 has been identified as a regulator of abiotic stress responses and promoter of secondary wall deposition in Betula platyphylla.To investigate the downstream targets of BplMYB46,the ex... The transcription factor BplMYB46 has been identified as a regulator of abiotic stress responses and promoter of secondary wall deposition in Betula platyphylla.To investigate the downstream targets of BplMYB46,the expression profiles of genes in stems from BplMYB46-overexpressing(OE)and BplMYB46-silencing(SE)plants were studied.In OE stems,952 genes were upregulated,and 1469 were downregulated in comparison to SE stems.In a KEGG pathway enrichment analysis of differentially expressed genes(DEGs),1387 differentially expressed genes were annotated for 117 metabolic pathways.DEGs were abundant for metabolic pathway,secondary metabolite biosynthesis,plant hormone signal transduction,phenylpropanoid and flavonoid biosynthesis.DEGs were implicated lignin or cellulose biosynthesis,cell wall modification,xylem development,disease resistance,stress responses,and anthocyanin biosynthesis.These results suggested that BplMYB46 regulates cell wall development and stress resistance by affecting the expression of these genes.Our study further elucidates the mechanism by which BplMYB46 mediates abiotic stress responses and secondary cell wall biosynthesis in birch. 展开更多
关键词 BETULA platyphylla BplMYB46 The GENE EXPRESSION profiles DIFFERENTIALLY EXPRESSED GENES
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Metabolite variation in hybrid corn grain from a large-scale multisite study
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作者 Mingjie Chen R.Shyama Prasad Rao +2 位作者 yiming zhang Cathy Zhong Jay J.Thelen 《The Crop Journal》 SCIE CAS CSCD 2016年第3期177-187,共11页
Metabolite composition is strongly affected by genotype,environment,and interactions between genotype and environment,although the extent of variation caused by these factors may depend upon the type of metabolite.To ... Metabolite composition is strongly affected by genotype,environment,and interactions between genotype and environment,although the extent of variation caused by these factors may depend upon the type of metabolite.To characterize the complexity of genotype,environment,and their interaction in hybrid seeds,50 genetically diverse non-genetically modified(GM) maize hybrids were grown in six geographically diverse locations in North America.Polar metabolites from 553 harvested corn grain samples were isolated and analyzed by gas chromatography–mass spectrometry and 45 metabolites detected in all samples were used to generate a data matrix for statistical analysis.There was moderate variation among biological replicates and across genotypes and test sites.The genotype effects were detected by univariate and Hierarchical clustering analyses(HCA) when environmental effects were excluded.Overall,environment exerted larger effects than genotype,and polar metabolite accumulation showed a geographic effect.We conclude that it is possible to increase seed polar metabolite content in hybrid corn by selection of appropriate inbred lines and growing regions. 展开更多
关键词 CORN GRAIN GENOTYPE Environment GC–MS Metabolomics
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光控诱导重组系统的开发与应用
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作者 谢甜 王梅 +3 位作者 高瑞钰 苗艳尼 张燚铭 蒋婧 《遗传》 CAS CSCD 北大核心 2022年第8期655-671,共17页
位点特异性重组系统由重组酶和特异性识别位点两部分组成,是一种强大的基因操作工具,被广泛运用于生命科学研究。已开发的诱导型重组系统以时空方式精准调控细胞和动物的基因表达,被用于基因功能研究、细胞谱系示踪和疾病治疗等领域。... 位点特异性重组系统由重组酶和特异性识别位点两部分组成,是一种强大的基因操作工具,被广泛运用于生命科学研究。已开发的诱导型重组系统以时空方式精准调控细胞和动物的基因表达,被用于基因功能研究、细胞谱系示踪和疾病治疗等领域。根据诱导重组酶时空表达方式的不同,诱导型重组系统可分为化学诱导和光控诱导两种方式。光控诱导重组系统是利用光作为诱导剂,根据光控方式和对象的不同,可进一步分为光笼和光遗传学两类。光笼诱导重组系统是利用光敏基团来控制化学诱导剂或重组酶,光诱导前它们的活性被光敏基团抑制;在特定光照射后,它们的活性被恢复,进而实现光控诱导基因重组。光遗传学诱导重组系统是通过光遗传学开关介导分割型重组酶的重新激活来诱导基因重组。其中光遗传学开关由一系列基因编码的光敏蛋白组成,包括隐花色素、VIVID蛋白、光敏色素等。这些类型丰富的光控诱导重组系统为从高时空分辨率的维度解析基因的表达和功能提供了更多的工具,以满足日益复杂的生命科学研究需求。本文主要对不同类型光控诱导重组系统的开发原理及应用进行综述,比较其优缺点,最后对未来开发更多光控重组系统进行展望,旨在为系统优化升级提供理论基础和指导。 展开更多
关键词 光控诱导重组系统 光笼 光遗传学开关 位点特异性重组酶 时空调控
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Strain-modulated ultrafast magneto-optic dynamics of graphene nanoflakes decorated with transition-metal atoms
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作者 张一鸣 刘景 +3 位作者 李春 金蔚 Georgios Lefkidis Wolfgang Hubner 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第9期520-527,共8页
We perform first-principles calculations and coherent laser-matter interaction analyses to investigate the laser-induced ultrafast spin flip on graphene nanoflakes(GNFs)with transition metal elements attached on the b... We perform first-principles calculations and coherent laser-matter interaction analyses to investigate the laser-induced ultrafast spin flip on graphene nanoflakes(GNFs)with transition metal elements attached on the boundary[TM&GNFs(TM=Fe,Co,Ni)].It is shown that the spin-flip process on TM&GNFs is highly influenced by the involved element species and the position attached to the nanoflakes.Furthermore,taking Ni&GNF as an example,the first-principles tensile test predicts that the variation of the C-Ni bond length plays an important role in the spin density distribution,especially for the low-lying magnetic states,and can therefore dominate the spin-flip processes.The fastest spin-flip scenario is achieved within 80 fs in a Ni&GNF structure under 10%tensile strain along the C-Ni bond.The local deformation modulation of spin flip provides the precursory guidance for further study of ultrafast magnetization control in GNFs,which could lead to potential applications in future integrated straintronic devices. 展开更多
关键词 graphene nanoflakes straintronics spin dynamics strain effect first principles
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Fast identification of mural pigments at Mogao Grottoes using a LIBS-based spectral matching algorithm
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作者 张一鸣 孙对兄 +4 位作者 殷耀鹏 于宗仁 苏伯民 董晨钟 苏茂根 《Plasma Science and Technology》 SCIE EI CAS CSCD 2022年第8期23-31,共9页
To quickly identify the mineral pigments in the Dunhuang murals,a spectral matching algorithm(SMA)based on four methods was combined with laser-induced breakdown spectroscopy(LIBS)for the first time.The optimal range ... To quickly identify the mineral pigments in the Dunhuang murals,a spectral matching algorithm(SMA)based on four methods was combined with laser-induced breakdown spectroscopy(LIBS)for the first time.The optimal range of LIBS spectrum for mineral pigments was determined using the similarity value between two different types of samples of the same pigment.A mineral pigment LIBS database was established by comparing the spectral similarities of tablets and simulated samples,and this database was successfully used to identify unknown pigments on tablet,simulated,and real mural debris samples.The results show that the SMA method coupled with the LIBS technique has great potential for identifying mineral pigments. 展开更多
关键词 mural pigments laser-induced breakdown spectroscopy fast identification and classification spectral matching algorithm spectral database
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