B cell activating factor belonging to TNF superfamily （BAFF） is a critical regulator of B cell maturation and survival In this present study, the expression characteristic of BAFF in kidney transplantation recipient...B cell activating factor belonging to TNF superfamily （BAFF） is a critical regulator of B cell maturation and survival In this present study, the expression characteristic of BAFF in kidney transplantation recipients was investigated, its potential significance was analyzed and peripheral blood of follow-up kidney transplant recipients was studied. Flow cytometric assay results showed that, cell-surface BAFF was significantly highly expressed on peripheral CD3＋ T lymphocytes in 〉 5 yrs group of kidney transplant recipients, compared with other groups （p 〈 0.05）. BAFF expression could be found on CD4＋ T cells and CD8＋ T cells. The BAFF mRNA levels in peripheral mononuclear cells were consistent with the protein levels. However, serum soluble BAFF levels were inter- individually different in each group. Stratified by renal function, it was found that cell-surface BAFF levels were significantly higher in those with abnormal renal function, compared with recipients with normal renal function （p 〈 0.05）. ELISA assay results showed that expression levels of cell surface BAFF were significantly correlated with anti-HLA I ＆ II antibodies. These results indicate that BAFF may be involved in the development of graft-loss and influences the long-time outcome of kidney allografl, likely mediated by interfering with immune response. Cellular ＆ Molecular Immunology. 2008;5（6）：465-470.展开更多
Supercritical carbon dioxide(SC-CO_(2))extraction of Conjugated linoleic acid(CLA)ethyl ester was investigated at pressures in the range of 9 to 10.5 MPa and temperature gradients ranging from 0℃ to 21℃.The content ...Supercritical carbon dioxide(SC-CO_(2))extraction of Conjugated linoleic acid(CLA)ethyl ester was investigated at pressures in the range of 9 to 10.5 MPa and temperature gradients ranging from 0℃ to 21℃.The content of CLA-ethyl ester in the fraction was analyzed with gas chromatography(GC).The experimental results indicated that the rate of extraction would rise with the increase of pressure when temperature gradient was given.Moreover,the extraction pressure had insignificant influence on the selectivity of CLA-ethyl ester in SCCO_(2).When pressure was fixed,setting certain temperature gradient can improve the selectivity of CLA-ethyl ester in SC-CO_(2),and CLA-ethyl ester can be concentrated more effectively than without a temperature gradient.The acid value and peroxide value of the fractions were reduced obviously,compared to the raw material.The optimal condition is pressure at 10 MPa and temperature gradient at 11℃.展开更多
基金supported by grants from the 135 Important Medical Project of Health Department of Jiangsu Province (No. K0621)
文摘B cell activating factor belonging to TNF superfamily （BAFF） is a critical regulator of B cell maturation and survival In this present study, the expression characteristic of BAFF in kidney transplantation recipients was investigated, its potential significance was analyzed and peripheral blood of follow-up kidney transplant recipients was studied. Flow cytometric assay results showed that, cell-surface BAFF was significantly highly expressed on peripheral CD3＋ T lymphocytes in 〉 5 yrs group of kidney transplant recipients, compared with other groups （p 〈 0.05）. BAFF expression could be found on CD4＋ T cells and CD8＋ T cells. The BAFF mRNA levels in peripheral mononuclear cells were consistent with the protein levels. However, serum soluble BAFF levels were inter- individually different in each group. Stratified by renal function, it was found that cell-surface BAFF levels were significantly higher in those with abnormal renal function, compared with recipients with normal renal function （p 〈 0.05）. ELISA assay results showed that expression levels of cell surface BAFF were significantly correlated with anti-HLA I ＆ II antibodies. These results indicate that BAFF may be involved in the development of graft-loss and influences the long-time outcome of kidney allografl, likely mediated by interfering with immune response. Cellular ＆ Molecular Immunology. 2008;5（6）：465-470.
基金supported by Key Laboratory for Green Chemical Process of Ministry of Education,Wuhan Institute of Technology,and the authors would like to acknowledge their financial support.
文摘Supercritical carbon dioxide(SC-CO_(2))extraction of Conjugated linoleic acid(CLA)ethyl ester was investigated at pressures in the range of 9 to 10.5 MPa and temperature gradients ranging from 0℃ to 21℃.The content of CLA-ethyl ester in the fraction was analyzed with gas chromatography(GC).The experimental results indicated that the rate of extraction would rise with the increase of pressure when temperature gradient was given.Moreover,the extraction pressure had insignificant influence on the selectivity of CLA-ethyl ester in SCCO_(2).When pressure was fixed,setting certain temperature gradient can improve the selectivity of CLA-ethyl ester in SC-CO_(2),and CLA-ethyl ester can be concentrated more effectively than without a temperature gradient.The acid value and peroxide value of the fractions were reduced obviously,compared to the raw material.The optimal condition is pressure at 10 MPa and temperature gradient at 11℃.
