Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety.Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L.monocytogenes by int...Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety.Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L.monocytogenes by interacting with the cell membrane.This research further explored the antibacterial mechanism of brevilaterin B against L.monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis.Brevilaterin B induced growth inhibition rather than direct membrane lysis in L.monocytogenes at the minimum inhibitory concentration.Transcriptomic analysis showed 1779 difference expressed genes,including 895 up-regulated and 884 down-regulated genes.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L.monocytogenes,including peptidoglycan biosynthesis,membrane transport(ATP-binding cassette transports,ion transport),cellular metabolism(amino acid and lipid metabolism),ATP synthesis,and activation of the stress response(quorum sensing and bacterial chemotaxis).In conclusion,brevilaterin B affects gene expression related to biosynthesis,transport and stress response pathways on the membrane of L.monocytogenes.The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level.展开更多
This paper deals with the iterative learning control （ILC） design for multiple-input multiple-output （MIMO）,time-delay systems （TDS）.Two feedback ILC schemes are considered using the so-called two-dimensional ...This paper deals with the iterative learning control （ILC） design for multiple-input multiple-output （MIMO）,time-delay systems （TDS）.Two feedback ILC schemes are considered using the so-called two-dimensional （2D） analysis approach.It shows that continuous-discrete 2D Roesser systems can be developed to describe the entire learning dynamics of both ILC schemes,based on which necessary and sufficient conditions for their stability can be provided.A numerical example is included to validate the theoretical analysis.展开更多
The aim of this paper is to solve the problems of multitarget tracking in clutter. Firstly, the data association of measurement-to-target is formulated as an integer programming problem. Through using the linear progr...The aim of this paper is to solve the problems of multitarget tracking in clutter. Firstly, the data association of measurement-to-target is formulated as an integer programming problem. Through using the linear programming （LP） based branchand-bound method and adjusting the constraint conditions, an optimal set integer programming （OSIP） algorithm is then proposed for tracking multiple non-maneuvering targets in clutter. For the case of maneuvering targets, this paper introduces the OSIP algorithm into the filtering step of the interacting multiple model （IMM） algorithm resulting in the IMM based on OSIP algorithm. Extensive Monte Carlo simulations show that the presented algorithms can obtain superior estimations even in the case of high density noises.展开更多
Background:Atopic dermatitis(AD)affects approximately 10%of adults worldwide.CM310 is a humanized monoclonal antibody targeting interleukin-4 receptor alpha that blocks interleukin-4 and interleukin-13 signaling.This ...Background:Atopic dermatitis(AD)affects approximately 10%of adults worldwide.CM310 is a humanized monoclonal antibody targeting interleukin-4 receptor alpha that blocks interleukin-4 and interleukin-13 signaling.This trial aimed to evaluate the efficacy and safety of CM310 in Chinese adults with moderate-to-severe AD.Methods:This multicenter,randomized,double-blind,placebo-controlled,phase 2b trial was conducted in 21 medical institutions in China from February to November 2021.Totally 120 eligible patients were enrolled and randomized(1:1:1)to receive subcutaneous injections of 300 mg CM310,150 mg CM310,or placebo every 2 weeks for 16 weeks,followed by an 8-week follow-up period.The primary endpoint was the proportion of patients achieving≥75%improvement in the Eczema Area and Severity Index(EASI-75)score from baseline at week 16.Safety and pharmacodynamics were also studied.Results:At week 16,the proportion of EASI-75 responders from baseline was significantly higher in the CM310 groups(70%[28/40]for high-dose and 65%[26/40]for low-dose)than that in the placebo group(20%[8/40]).The differences in EASI-75 response rate were 50%(high vs.placebo,95%CI 31%-69%)and 45%(low vs.placebo,95%CI 26%-64%),with both P values<0.0001.CM310 at both doses also significantly improved the EASI score,Investigator’s Global Assessment score,daily peak pruritus Numerical Rating Scale,AD-affected body surface area,and Dermatology Life Quality Index compared with placebo.CM310 treatment reduced levels of thymus and activation-regulated chemokine,total immunoglobulin E,lactate dehydrogenase,and blood eosinophils.The incidence of treatment-emergent adverse events(TEAEs)was similar among all three groups,with the most common TEAEs reported being upper respiratory tract infection,atopic dermatitis,hyperlipidemia,and hyperuricemia.No severe adverse events were deemed to be attributed to CM310.Conclusion:CM310 at 150 mg and 300 mg every 2 weeks demonstrated significant efficacy and was well-tolerated in adults with moderate-to-severe AD.Trial Registration:ClinicalTrials.gov,NCT04805411.展开更多
Walnuts are rich in protein and are a high-quality plant protein resource.In recent years,with the growth of consumer demand for functional food and food for special medical purpose,the use of walnut protein for the p...Walnuts are rich in protein and are a high-quality plant protein resource.In recent years,with the growth of consumer demand for functional food and food for special medical purpose,the use of walnut protein for the preparation of functional walnut peptide ingredients or additives and other compositions has received increasing attention.However,the improvement of the yield of walnut peptides and the clarification of their functional activities are the bottlenecks that limit the development of these peptides.To this end,this article reviews the pretreatment,preparation,purification and identification processes of walnut peptides,as well as their biological activities such as antioxidant activity,antitumour activity,improvement of memory,antihypertension and regulation of metabolic disorders are elaborated to provide a reference for the industrial development of walnut peptides.展开更多
This note is devoted to the problem of robust stability of interval parameter matrices. Based on some basic facts relating the H∞ norm of a transfer function to the Riccati matrixinequality and Hamilton matrix, sever...This note is devoted to the problem of robust stability of interval parameter matrices. Based on some basic facts relating the H∞ norm of a transfer function to the Riccati matrixinequality and Hamilton matrix, several test conditions with parameter perturbation bounds are obtained.展开更多
In this paper,the conventional tracking control problem is expanded to first-order multi-agent systems,which can be solved by directly guiding any agent in the group.The following three kinds of desired motions are co...In this paper,the conventional tracking control problem is expanded to first-order multi-agent systems,which can be solved by directly guiding any agent in the group.The following three kinds of desired motions are considered for all agents to track:1)stillness in space,2)variable motion with known acceleration,3)variable motion with partly unknown acceleration.Specifically,fixed networks with time delays and switching networks without delays are both considered in case 1).Switching networks with and without time delays are both studied in case 2),while for 3),switching networks without delays are mainly investigated.A numerical simulation example is included to illustrate the results.展开更多
基金financially supported by the National Natural Science Foundation of China(31771951,32072199,31801510)the Beijing Natural Science Foundation(KZ201810011016).
文摘Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety.Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L.monocytogenes by interacting with the cell membrane.This research further explored the antibacterial mechanism of brevilaterin B against L.monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis.Brevilaterin B induced growth inhibition rather than direct membrane lysis in L.monocytogenes at the minimum inhibitory concentration.Transcriptomic analysis showed 1779 difference expressed genes,including 895 up-regulated and 884 down-regulated genes.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L.monocytogenes,including peptidoglycan biosynthesis,membrane transport(ATP-binding cassette transports,ion transport),cellular metabolism(amino acid and lipid metabolism),ATP synthesis,and activation of the stress response(quorum sensing and bacterial chemotaxis).In conclusion,brevilaterin B affects gene expression related to biosynthesis,transport and stress response pathways on the membrane of L.monocytogenes.The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level.
基金supported by the National Natural Science Foundation of China(No.60727002,60774003,60921001,90916024)the COSTIND(No.A2120061303)the National 973 Program(No.2005CB321902)
文摘This paper deals with the iterative learning control （ILC） design for multiple-input multiple-output （MIMO）,time-delay systems （TDS）.Two feedback ILC schemes are considered using the so-called two-dimensional （2D） analysis approach.It shows that continuous-discrete 2D Roesser systems can be developed to describe the entire learning dynamics of both ILC schemes,based on which necessary and sufficient conditions for their stability can be provided.A numerical example is included to validate the theoretical analysis.
基金supported by the National Natural Science Fundation of China (61203238 61134005+5 种基金 60921001 90916024 91116016)the National Basic Research Program of China (973 Program) (2012CB8212002012CB821201)the National Science Foundation for Postdoctoral Scientists of China (2012M520140)
文摘The aim of this paper is to solve the problems of multitarget tracking in clutter. Firstly, the data association of measurement-to-target is formulated as an integer programming problem. Through using the linear programming （LP） based branchand-bound method and adjusting the constraint conditions, an optimal set integer programming （OSIP） algorithm is then proposed for tracking multiple non-maneuvering targets in clutter. For the case of maneuvering targets, this paper introduces the OSIP algorithm into the filtering step of the interacting multiple model （IMM） algorithm resulting in the IMM based on OSIP algorithm. Extensive Monte Carlo simulations show that the presented algorithms can obtain superior estimations even in the case of high density noises.
基金funded by the National 13th Five-Year Plan for Major New Drug Development of China(No.2017ZX09302010)
文摘Background:Atopic dermatitis(AD)affects approximately 10%of adults worldwide.CM310 is a humanized monoclonal antibody targeting interleukin-4 receptor alpha that blocks interleukin-4 and interleukin-13 signaling.This trial aimed to evaluate the efficacy and safety of CM310 in Chinese adults with moderate-to-severe AD.Methods:This multicenter,randomized,double-blind,placebo-controlled,phase 2b trial was conducted in 21 medical institutions in China from February to November 2021.Totally 120 eligible patients were enrolled and randomized(1:1:1)to receive subcutaneous injections of 300 mg CM310,150 mg CM310,or placebo every 2 weeks for 16 weeks,followed by an 8-week follow-up period.The primary endpoint was the proportion of patients achieving≥75%improvement in the Eczema Area and Severity Index(EASI-75)score from baseline at week 16.Safety and pharmacodynamics were also studied.Results:At week 16,the proportion of EASI-75 responders from baseline was significantly higher in the CM310 groups(70%[28/40]for high-dose and 65%[26/40]for low-dose)than that in the placebo group(20%[8/40]).The differences in EASI-75 response rate were 50%(high vs.placebo,95%CI 31%-69%)and 45%(low vs.placebo,95%CI 26%-64%),with both P values<0.0001.CM310 at both doses also significantly improved the EASI score,Investigator’s Global Assessment score,daily peak pruritus Numerical Rating Scale,AD-affected body surface area,and Dermatology Life Quality Index compared with placebo.CM310 treatment reduced levels of thymus and activation-regulated chemokine,total immunoglobulin E,lactate dehydrogenase,and blood eosinophils.The incidence of treatment-emergent adverse events(TEAEs)was similar among all three groups,with the most common TEAEs reported being upper respiratory tract infection,atopic dermatitis,hyperlipidemia,and hyperuricemia.No severe adverse events were deemed to be attributed to CM310.Conclusion:CM310 at 150 mg and 300 mg every 2 weeks demonstrated significant efficacy and was well-tolerated in adults with moderate-to-severe AD.Trial Registration:ClinicalTrials.gov,NCT04805411.
基金supports from the National Key Research and Development Program of China(CN)(2022YFD1600402)Hebei Provincial Major Science and Technology Achievement Transformation Project(CN)(21287101Z)+1 种基金Hebei Provincial Innovation and Entrepreneurship Team Project(CN)(215A7102D)Discipline Construction-Food Science and Engineering(CN)(SPCX-2022005).
文摘Walnuts are rich in protein and are a high-quality plant protein resource.In recent years,with the growth of consumer demand for functional food and food for special medical purpose,the use of walnut protein for the preparation of functional walnut peptide ingredients or additives and other compositions has received increasing attention.However,the improvement of the yield of walnut peptides and the clarification of their functional activities are the bottlenecks that limit the development of these peptides.To this end,this article reviews the pretreatment,preparation,purification and identification processes of walnut peptides,as well as their biological activities such as antioxidant activity,antitumour activity,improvement of memory,antihypertension and regulation of metabolic disorders are elaborated to provide a reference for the industrial development of walnut peptides.
文摘This note is devoted to the problem of robust stability of interval parameter matrices. Based on some basic facts relating the H∞ norm of a transfer function to the Riccati matrixinequality and Hamilton matrix, several test conditions with parameter perturbation bounds are obtained.
基金supported by the National Natural Science Foundation of China (Grant Nos.60774003,60727002)the National Basic Research Program of China (No.2005CB321902)the Commission of Science,Technology and Industry for National Defense (No.A2120061303).
文摘In this paper,the conventional tracking control problem is expanded to first-order multi-agent systems,which can be solved by directly guiding any agent in the group.The following three kinds of desired motions are considered for all agents to track:1)stillness in space,2)variable motion with known acceleration,3)variable motion with partly unknown acceleration.Specifically,fixed networks with time delays and switching networks without delays are both considered in case 1).Switching networks with and without time delays are both studied in case 2),while for 3),switching networks without delays are mainly investigated.A numerical simulation example is included to illustrate the results.
