Microneurovascular units(mNVUs),comprising neurons,micro-glia,and blood-brain barrier(BBB)endothelial cells,are pivotal to the central nervous system and are associated with cerebral hypoxia and brain injuries.Cerebra...Microneurovascular units(mNVUs),comprising neurons,micro-glia,and blood-brain barrier(BBB)endothelial cells,are pivotal to the central nervous system and are associated with cerebral hypoxia and brain injuries.Cerebral hypoxia triggers microglial overactivity,causing inflammation,neuronal injury,and disruption of the BBB[1].Salidroside(Sal),a key compound in Tibetan medicine Rhodiola crenulata,mitigates hypoxia-induced metabolic disorders and neuronal damage by preserving mitochondrial function[2].展开更多
Aconitine,a common and main toxic component of Aconitum,is toxic to the central nervous system.However,the mechanism of aconitine neurotoxicity is not yet clear.In this work,we had the hypothesis that excitatory amino...Aconitine,a common and main toxic component of Aconitum,is toxic to the central nervous system.However,the mechanism of aconitine neurotoxicity is not yet clear.In this work,we had the hypothesis that excitatory amino acids can trigger excitotoxicity as a pointcut to explore the mechanism of neurotoxicity induced by aconitine.HT22 cells were simulated by aconitine and the changes of target cell metabolites were real-time online investigated based on a microfluidic chip-mass spectrometry system.Meanwhile,to confirm the metabolic mechanism of aconitine toxicity on HT22 cells,the levels of lactate dehydrogenase,intracellular Ca^(2+),reactive oxygen species,glutathione and superoxide dismutase,and ratio of Bax/Bcl-2 protein were detected by molecular biotechnology.Integration of the detected results revealed that neurotoxicity induced by aconitine was associated with the process of excitotoxicity caused by glutamic acid and aspartic acid,which was followed by the accumulation of lactic acid and reduction of glucose.The surge of extracellular glutamic acid could further lead to a series of cascade reactions including intracellular Ca^(2+)overload and oxidative stress,and eventually result in cell apoptosis.In general,we illustrated a new mechanism of aconitine neurotoxicity and presented a novel analysis strategy that real-time online monitoring of cell metabolites can provide a new approach to mechanism analysis.展开更多
Stress corrosion cracking （SCC） of X70 pipeline steel in simulated solution of the acidic soil in Yingtan in China was investigated using slow strain rate test （SSRT）, SEM and potentiodynamic polarization techniqu...Stress corrosion cracking （SCC） of X70 pipeline steel in simulated solution of the acidic soil in Yingtan in China was investigated using slow strain rate test （SSRT）, SEM and potentiodynamic polarization technique. Experiment results indicate that X70 steel is highly susceptible to SCC as applied potential reduces, which is manifested in loss of toughness and brittle fracture. Constaat polarization current can detect the occurrence of SCC. The lower the polarization current is the sooner stress corrosion cracking occurs. The SCC mechanisms are different at varying potentials. When potential is higher than open circuit potential, anodic process controls SCC, whereas when potential is far lower than open circuit potential, cathodic process controls SCC, and between these two potential regions, a combined electrochemical process controls the SCC. Stress or strain has a synergistic effect with electrochemical reactions to accelerate the cathodic hydrogen evolution process, which makes the X70 pipeline steel to be more susceptible to SCC.展开更多
Background:The outbreak of coronavirus disease 2019(COVID-19)has posed a huge threat to human health.However,little is known regarding the risk factors associated with COVID-19 severity.We aimed to explore early-stage...Background:The outbreak of coronavirus disease 2019(COVID-19)has posed a huge threat to human health.However,little is known regarding the risk factors associated with COVID-19 severity.We aimed to explore early-stage disease risk factors associated with eventual disease severity.Methods:This study enrolled 486 hospitalized,non-intensive care unit(ICU)-admitted adult patients with COVID-19(age≥18 years)treated at Wuhan Jinyintan Hospital,who were divided into three groups according to disease severity.The demographic,clinical,and laboratory data at admission and clinical outcomes were compared among severity groups,and the risk factors for disease severity were identified by multiple regression analysis.Results:Of 486 patients with COVID-19,405(83.33%)were discharged,33(6.71%)died outside of the ICU,and 48(7.20%)were still being treated in the ICU by the time the study period ended.Significant differences in age,lymphocyte counts,and the levels of procalcitonin,aspartate aminotransferase,and D-dimer(P<0.001 for all)among the three groups.Further analysis showed that older age,decreased lymphocyte counts,and increased procalcitonin,aspartate aminotransferase,and D-dimer levels were significantly associated with disease progression.Conclusion:Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)may impair the immune system,the blood coagulation system,and hepatic and cardiac function.Some clinical characteristics and laboratory findings can help identify patients with a high risk of disease severity,which can be significant for appropriate resource allocation during the COVID-19 pandemic.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.:82274207,81973569,22034005)the Xinglin Scholar Research Promotion Project of Chengdu University of Traditional Chinese Medicine,China(Grant No.:XKTD2022013)the Sichuan Provincial Natural Science Foundation,China(Grant No.:24NSFSC1748).
文摘Microneurovascular units(mNVUs),comprising neurons,micro-glia,and blood-brain barrier(BBB)endothelial cells,are pivotal to the central nervous system and are associated with cerebral hypoxia and brain injuries.Cerebral hypoxia triggers microglial overactivity,causing inflammation,neuronal injury,and disruption of the BBB[1].Salidroside(Sal),a key compound in Tibetan medicine Rhodiola crenulata,mitigates hypoxia-induced metabolic disorders and neuronal damage by preserving mitochondrial function[2].
基金supported the National Natural Science Foundation of China(Grant Nos.:81973569,82130113,and 22034005)the National Key R&D Program of China(Grant No.:2021YFF0600700)the“Xinglin Scholars”Research Promotion Program of Chengdu University of Traditional Chinese Medicine(Grant No.:BSH2021009).
文摘Aconitine,a common and main toxic component of Aconitum,is toxic to the central nervous system.However,the mechanism of aconitine neurotoxicity is not yet clear.In this work,we had the hypothesis that excitatory amino acids can trigger excitotoxicity as a pointcut to explore the mechanism of neurotoxicity induced by aconitine.HT22 cells were simulated by aconitine and the changes of target cell metabolites were real-time online investigated based on a microfluidic chip-mass spectrometry system.Meanwhile,to confirm the metabolic mechanism of aconitine toxicity on HT22 cells,the levels of lactate dehydrogenase,intracellular Ca^(2+),reactive oxygen species,glutathione and superoxide dismutase,and ratio of Bax/Bcl-2 protein were detected by molecular biotechnology.Integration of the detected results revealed that neurotoxicity induced by aconitine was associated with the process of excitotoxicity caused by glutamic acid and aspartic acid,which was followed by the accumulation of lactic acid and reduction of glucose.The surge of extracellular glutamic acid could further lead to a series of cascade reactions including intracellular Ca^(2+)overload and oxidative stress,and eventually result in cell apoptosis.In general,we illustrated a new mechanism of aconitine neurotoxicity and presented a novel analysis strategy that real-time online monitoring of cell metabolites can provide a new approach to mechanism analysis.
基金supported by Chinese National Science and Technology Infrastructure Platforms Construction Project (No.2005DKA10400)Major Foundation in the Tenth Five-Year Development Plan of China (No.50499333-08)
文摘Stress corrosion cracking （SCC） of X70 pipeline steel in simulated solution of the acidic soil in Yingtan in China was investigated using slow strain rate test （SSRT）, SEM and potentiodynamic polarization technique. Experiment results indicate that X70 steel is highly susceptible to SCC as applied potential reduces, which is manifested in loss of toughness and brittle fracture. Constaat polarization current can detect the occurrence of SCC. The lower the polarization current is the sooner stress corrosion cracking occurs. The SCC mechanisms are different at varying potentials. When potential is higher than open circuit potential, anodic process controls SCC, whereas when potential is far lower than open circuit potential, cathodic process controls SCC, and between these two potential regions, a combined electrochemical process controls the SCC. Stress or strain has a synergistic effect with electrochemical reactions to accelerate the cathodic hydrogen evolution process, which makes the X70 pipeline steel to be more susceptible to SCC.
基金supported by the Fujian Provincial Intensive Medical Center Construction Project[2017-510].
文摘Background:The outbreak of coronavirus disease 2019(COVID-19)has posed a huge threat to human health.However,little is known regarding the risk factors associated with COVID-19 severity.We aimed to explore early-stage disease risk factors associated with eventual disease severity.Methods:This study enrolled 486 hospitalized,non-intensive care unit(ICU)-admitted adult patients with COVID-19(age≥18 years)treated at Wuhan Jinyintan Hospital,who were divided into three groups according to disease severity.The demographic,clinical,and laboratory data at admission and clinical outcomes were compared among severity groups,and the risk factors for disease severity were identified by multiple regression analysis.Results:Of 486 patients with COVID-19,405(83.33%)were discharged,33(6.71%)died outside of the ICU,and 48(7.20%)were still being treated in the ICU by the time the study period ended.Significant differences in age,lymphocyte counts,and the levels of procalcitonin,aspartate aminotransferase,and D-dimer(P<0.001 for all)among the three groups.Further analysis showed that older age,decreased lymphocyte counts,and increased procalcitonin,aspartate aminotransferase,and D-dimer levels were significantly associated with disease progression.Conclusion:Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)may impair the immune system,the blood coagulation system,and hepatic and cardiac function.Some clinical characteristics and laboratory findings can help identify patients with a high risk of disease severity,which can be significant for appropriate resource allocation during the COVID-19 pandemic.
