The siRNA-loaded lipid nanoparticles have attracted much attention due to its significant gene silencing effect and successful marketization.However,the in vivo distribution and release of siRNA still cannot be effect...The siRNA-loaded lipid nanoparticles have attracted much attention due to its significant gene silencing effect and successful marketization.However,the in vivo distribution and release of siRNA still cannot be effectively monitored.In this study,based on the fluorescence resonance energy transfer(FRET)principle,a fluorescence dye Cy5-modified survivin siRNA was conjugated to nanogolds(Au-DR-siRNA),which were then wrapped with lipid nanoparticles(LNPs)for monitoring the release behaviour of siRNA in vivo.The results showed that once Au-DR-siRNA was released from the LNPs and cleaved by the Dicer enzyme to produce free siRNA in cells,the fluorescence of Cy5 would change from quenched state to activated state,showing the location and time of siRNA release.Besides,the LNPs showed a significant antitumor effect by silencing the survivin gene and a CT imaging function superior to iohexol by nanogolds.Therefore,this work provided not only an effective method for monitoring the pharmacokinetic behaviour of LNP-based siRNA,but also a siRNA delivery system for treating and diagnosing tumors.展开更多
The strain LWYZ003, which can restrain multiple pathogens, was screened from the sediment of the ocean and identified as Bacillus amyloliquefaciens. Large-scale fermentation and modern chromatographic separation techn...The strain LWYZ003, which can restrain multiple pathogens, was screened from the sediment of the ocean and identified as Bacillus amyloliquefaciens. Large-scale fermentation and modern chromatographic separation technologies(macroporous resin column chromatography, silica gel column chromatography, thin-layer chromatography and high-performance liquid chromatography) were used to separate antimicrobial products from the fermentation broth of marine-derived Bacillus amyloliquefaciens LWYZ003. Bioactive-guided separation was used in the term of seeking antimicrobial products from the secondary metabolites of Bacillus amyloliquefaciens LWYZ003. As a result, two natural products cycloheximide( 1) and trehalose( 2) were obtained. Their structures were elucidated by Fourier transform infrared spectroscopy, high-resolution mass spectrometry, ~1 H and ^(13) C nuclear magnetic resonance analysis. In the cylinder plate method, compound 1 exhibited stronger antimicrobial activities than compound 2 against Micrococcus luteus, and also exhibited wider antimicrobial spectrum than compound 2. In conclusion, isolation of bioactive secondary metabolites from marine Bacillus sp. has enormous potentials in finding suitable antibiotics to inhibit multiple pathogens.展开更多
In recent years, the telecommunications have used the concept of NPS(Net Promoter Score) for customer relationship management, but there is neither definite theory research nor instructive instance research. However, ...In recent years, the telecommunications have used the concept of NPS(Net Promoter Score) for customer relationship management, but there is neither definite theory research nor instructive instance research. However, this paper summarizes an approach with instance case analysis to improve customer loyalty via NPS data mining, which has extensive and practical significance for tele-companies. First, this paper finds some driven forces of customer loyalty, which are relative to customer consumption such as the call duration, the usage of data, ARPU, etc., by using some innovative reasoning-analysis based on IG(Information Gain) and xg-boost decision-making tree model, so the tele-companies can predict the role of individual customer and form daily monitoring on big data, which will save a lot of NPS survey cost. Second, this paper summarizes how customer group feature impacts the relationship between NPS and financial performance. Taking ARPU value as the performance goals, we divide the sample customers into 6 groups and summarize their characteristics based on k-means clustering, and give targeted suggestion of each group.展开更多
Physical education teaching mode is a general teaching procedure that embodies certain teaching ideas and aims and organizesteaching by a series of teaching methods and means. Implementing the experiential teaching mo...Physical education teaching mode is a general teaching procedure that embodies certain teaching ideas and aims and organizesteaching by a series of teaching methods and means. Implementing the experiential teaching mode of college physical educationhas become an important part of the reform of College Physical Education in China. The university sports experiential teachingmode is an important form of teaching. It helps students to be independent and proactive in physical education. It is an importantway to cultivate innovative talents and improve the teaching effect of universities. Starting from the meaning and characteristics ofthe experiential teaching mode, this paper expounds the implementation significance of the current physical education teachingstatus and problems, and finally explores effective application strategies.展开更多
Metal-organic frameworks(MOFs)are a new class of crystalline porous materials with periodic network structures,which are formed by self-assembly between metal ions and bridging organic linkers.Due to high surface area...Metal-organic frameworks(MOFs)are a new class of crystalline porous materials with periodic network structures,which are formed by self-assembly between metal ions and bridging organic linkers.Due to high surface areas,large pore volumes,and tunable surface properties,MOFs present promising application in gas storage and separation,drug delivery,heterogeneous catalysis,etc.Recently,MOFs are found to展开更多
Brine shrimp(Artemia)has existed on Earth for 400 million years and has major ecological importance in hypersaline ecosystems.As a crucial live food in aquaculture,brine shrimp cysts have become one of the most import...Brine shrimp(Artemia)has existed on Earth for 400 million years and has major ecological importance in hypersaline ecosystems.As a crucial live food in aquaculture,brine shrimp cysts have become one of the most important aquatic products traded worldwide.However,our understanding of the biodiversity,prevalence and global connectedness of viruses in brine shrimp is still very limited.A total of 143 batches of brine shrimp(belonging to seven species)cysts were collected from six continents including 21 countries and more than 100 geographic locations worldwide during 1977–2019.In total,55 novel RNA viruses were identified,which could be assigned to 18 different viral families and related clades.Eleven viruses were dsRNA viruses,16 were+ssRNA viruses,and 28 were−ssRNA viruses.Phylogenetic analyses of the RNA-directed RNA polymerase(RdRp)showed that brine shrimp viruses were often grouped with viruses isolated from other invertebrates and fungi.Remarkably,most brine shrimp viruses were related to those from different hosts that might feed on brine shrimp or share the same ecological niche.A notable case was the novel brine shrimp noda-like virus 3,which shared 79.25%(RdRp)and 63.88%(capsid proteins)amino acid identity with covert mortality nodavirus(CMNV)that may cause losses in aquaculture.In addition,both virome composition and phylogenetic analyses revealed global connectedness in certain brine shrimp viruses,particularly among Asia and Northern America.This highlights the incredible species diversity of viruses in these ancient species and provides essential data for the prevalence of RNA viruses in the global aquaculture industry.More broadly,these findings provide novel insights into the previously unrecognized RNA virosphere in hypersaline ecosystems worldwide and demonstrate that human activity might have driven the global connectedness of brine shrimp viruses.展开更多
Toxic arsenic(As)and trace element selenium(Se)are transformed by microorganisms but their complex interactions in soil-plant systems have not been fully understood.An Asand Se-oxidizing bacterium,Agrobacterium sp.T3F...Toxic arsenic(As)and trace element selenium(Se)are transformed by microorganisms but their complex interactions in soil-plant systems have not been fully understood.An Asand Se-oxidizing bacterium,Agrobacterium sp.T3F4,was applied to a native seleniferous As-polluted soil to investigate As/Se uptake by the vegetable Brassica rapa L.and As-Se interaction as mediated by strain T3F4.The Se content in the aboveground plants was significantly enhanced by 34.1%,but the As content was significantly decreased by 20.5% in the T3F4-inoculated pot culture compared to the control(P<0.05).Similar result was shown in treatment with additional 5 mg/kg of Se(IV)in soil.In addition,the As contents in roots were significantly decreased by more than 35% under T3F4 or Se(IV)treatments(P<0.05).Analysis of As-Se-bacterium interaction in a soil simulation experiment showed that the bioavailability of Se significantly increased and As was immobilized with the addition of the T3F4strain(P<0.05).Furthermore,an As/Se co-exposure hydroponic experiment demonstrated that As uptake and accumulation in plants was reduced by increasing Se(IV)concentrations.The 50% growth inhibition concentration(IC50)values for As in plants were increased about one-fold and two-fold under co-exposure with 5 and 10μmol/L Se(IV),respectively.In conclusion,strain T3F4 improves Se uptake but decreases As uptake by plants via oxidation of As and Se,resulting in decrease of soil As bioavailability and As/Se competitive absorption by plants.This provides a potential bioremediation strategy for Se biofortification and As immobilization in As-polluted soil.展开更多
To the Editor:Upfront autologous stem cell transplantation(ASCT)has been an integral part of the management of young and eligible older patients with newly diagnosed multiple myeloma(NDMM)for over three decades.Tandem...To the Editor:Upfront autologous stem cell transplantation(ASCT)has been an integral part of the management of young and eligible older patients with newly diagnosed multiple myeloma(NDMM)for over three decades.Tandem stem cell transplantation was defined as two planned sequential ASCTs within 3 to 6 months.More than two decades after its introduction,trials exploring the utility of tandem transplants have yielded mixed results.The role of tandem ASCT in multiple myeloma(MM)remains unclear and controversial.In the EMN02/HOVON95 study.展开更多
Extracellular vesicles from highly metastatic tumor cells have been shown to mediate epithelial-mesenchymal transition(EMT)-related events in recipient cells.In endometrial epithelial cells,EMT processes are known to ...Extracellular vesicles from highly metastatic tumor cells have been shown to mediate epithelial-mesenchymal transition(EMT)-related events in recipient cells.In endometrial epithelial cells,EMT processes are known to be involved in the development of adenomyosis.We aimed to investigate whether adenomyosis-derived extracellular vesicles(AMEVs)are able to induce an EMT process in endometrial epithelial cells.In this study,AMEVs were isolated from patients with adenomyosis and characterized by transmission electron microscopy,Western blot,and nanoparticle tracking.Primary endometrial epithelial cells(EECs)were derived from normal endometrium tissues from patients with leiomyoma and co-cultured with AMEVs in vitro.AMEV uptake was examined by fluorescence confocal microscopy.The invasion of EECs was confirmed by Transwell assay.Immunohistochemistry,Western blot,and qRT-PCR were performed on EECs to illustrate the expression levels of cytokeratin 19,E-cadherin,vimentin,and zinc finger E-boxbinding homeobox 1(ZEB1).The results indicated that the cellular fluorescence intensity gradually increased after 48 h of co-culture,but decreased after 72 h.After co-culturing with AMEVs for 72 h,EECs expressed significantly lower levels of cytokeratin 19 and E-cadherin,and significantly higher levels of vimentin and ZEB1.Together these results demonstrated that AMEVs induce an EMT process and enhance the invasion of EECs.These changes may contribute to the pathogenesis and progression of adenomyosis.展开更多
Summary What is already known about this topic?There were a total of 4 and 3 human plague cases that occurred in the Inner Mongolia Autonomous Region in 2019 and 2020,respectively,with 1 and 2 deaths in 2019 and 2020 ...Summary What is already known about this topic?There were a total of 4 and 3 human plague cases that occurred in the Inner Mongolia Autonomous Region in 2019 and 2020,respectively,with 1 and 2 deaths in 2019 and 2020 respectively,which indicated that plague still poses a significant threat to human health especially for farmers,shepherds,or residents living in native plague foci.What is added by this report?On August 14,2021,1 patient from the Otog Qi(County)in the Inner Mongolia sought treatment in Yinchuan City(the capital of Ningxia Hui Autonomous Region).展开更多
An emergency platform is an informatization support platform for disaster information perception,disaster situa-tional awareness,and emergency decision command.This is a key tool for ensuring the efficiency and effect...An emergency platform is an informatization support platform for disaster information perception,disaster situa-tional awareness,and emergency decision command.This is a key tool for ensuring the efficiency and effectiveness of emergency responses.Numerous large countries have conducted in-depth research on the key technologies of emergency platforms.Over the past 20 years,with the reform of emergency management mechanisms,China has developed two generations of emergency platform systems.This paper reviews the two generations of emergency platforms and summarizes their key technologies including multi-source-based monitoring and early warning,multi-hazard risk assessment,“scenario-response”-based decision support,synthetical forecasting based on in-cident chain,and emergency common operational picture(COP)for command and dispatch.Future research directions for the next generation emergency platforms are also proposed.展开更多
The linearity assumption is widely used when acquiring the hydrodynamic coefficients of a floating structure.However,the linear damping is frequently underestimated,especially for the natural frequency.To investigate ...The linearity assumption is widely used when acquiring the hydrodynamic coefficients of a floating structure.However,the linear damping is frequently underestimated,especially for the natural frequency.To investigate the sloping seafloor effects on the damping terms of a single module of a semi-submersible Very Large Floating Structure(VLFS),this paper revisits the conventional formulation and further proposes the direct integration method for obtaining the linear and quadratic damping coefficients from free-decay tests.Numerical free-decay simulations of the single module over variable bathymetry are carried out by the CFD numerical tank.Corresponding model tests are also implemented to verify and validate against the numerical solutions.The effects of the sloping seafloor,as well as the water depth,on the hydrodynamic coefficients are investigated based on the validated CFD modeling.Both numerical and experimental results indicate that the acquisition of the linear and quadratic damping coefficients is sensitive to the data-processing and identification approaches.For the case studied in present paper,the identification errors introduced by the conventional method are 1.5%while they are 0.5%using the direct integration method.The quadratic damping coefficient for heave mode decreases about 10.4%when the sloping angle increases from 0 to 6 deg.展开更多
The coronavirus disease 2019(COVID-19)pandemic started in early 2020.At the beginning of February,a public welfare activity in epidemic data visualization,jointly launched by China Computer Federation(CCF)(CCF)CAD&...The coronavirus disease 2019(COVID-19)pandemic started in early 2020.At the beginning of February,a public welfare activity in epidemic data visualization,jointly launched by China Computer Federation(CCF)(CCF)CAD&CG Technical Committee,Alibaba Cloud Tianchi(Alibaba Cloud Tianch),JiqiZhixin(JiqiZhixin),Alibaba Cloud DataV(Alibaba Cloud DataV),and DataWhale(DataWhale),was launched with the theme‘‘Fighting the Epidemic with One Mind and Talents like Tianchi.’’Developers in general are expected to focus on several demand scenarios,such as epidemic situation display,epidemic popular science,trend prediction,material-supply situation,and rework and return situation of employees from all sectors and areas,to discover the relationship between complex heterogeneous multi-source data,develop various upbeat works and present useful information to the public in a coherent manner.The entry works take the form of data visualization and are divided into two categories:popular science publicity and application scenarios.The popular science publicity category includes works for the public,focused on epidemic situation display,epidemic popular science publicity,epidemic prevention and control,and others.The application scenario category consists of the works of frontline officers,which can provide anti-epidemic workers with effective data tools for efficient and intuitive epidemic analysis;offer reliable,understandable,and easily transmitted information for disease prevention;and assist governments,enterprises,and institutions in the fight against COVID-19.展开更多
基金by the National Natural Science Foundation of China(81872812,82073800)the China Postdoctoral Science Fundation(2021TQ0111,2021M691040).
文摘The siRNA-loaded lipid nanoparticles have attracted much attention due to its significant gene silencing effect and successful marketization.However,the in vivo distribution and release of siRNA still cannot be effectively monitored.In this study,based on the fluorescence resonance energy transfer(FRET)principle,a fluorescence dye Cy5-modified survivin siRNA was conjugated to nanogolds(Au-DR-siRNA),which were then wrapped with lipid nanoparticles(LNPs)for monitoring the release behaviour of siRNA in vivo.The results showed that once Au-DR-siRNA was released from the LNPs and cleaved by the Dicer enzyme to produce free siRNA in cells,the fluorescence of Cy5 would change from quenched state to activated state,showing the location and time of siRNA release.Besides,the LNPs showed a significant antitumor effect by silencing the survivin gene and a CT imaging function superior to iohexol by nanogolds.Therefore,this work provided not only an effective method for monitoring the pharmacokinetic behaviour of LNP-based siRNA,but also a siRNA delivery system for treating and diagnosing tumors.
基金supported by the National Basic Research Program of China ("973" Program) (no. 2012CB721105)the Major Research Plan of Tianjin, China (no. 16YFXTSF00460)
文摘The strain LWYZ003, which can restrain multiple pathogens, was screened from the sediment of the ocean and identified as Bacillus amyloliquefaciens. Large-scale fermentation and modern chromatographic separation technologies(macroporous resin column chromatography, silica gel column chromatography, thin-layer chromatography and high-performance liquid chromatography) were used to separate antimicrobial products from the fermentation broth of marine-derived Bacillus amyloliquefaciens LWYZ003. Bioactive-guided separation was used in the term of seeking antimicrobial products from the secondary metabolites of Bacillus amyloliquefaciens LWYZ003. As a result, two natural products cycloheximide( 1) and trehalose( 2) were obtained. Their structures were elucidated by Fourier transform infrared spectroscopy, high-resolution mass spectrometry, ~1 H and ^(13) C nuclear magnetic resonance analysis. In the cylinder plate method, compound 1 exhibited stronger antimicrobial activities than compound 2 against Micrococcus luteus, and also exhibited wider antimicrobial spectrum than compound 2. In conclusion, isolation of bioactive secondary metabolites from marine Bacillus sp. has enormous potentials in finding suitable antibiotics to inhibit multiple pathogens.
基金Supported by Humanities and Social Sciences Foundation of Ministry of Education in China (Project No. 16YJA630063)
文摘In recent years, the telecommunications have used the concept of NPS(Net Promoter Score) for customer relationship management, but there is neither definite theory research nor instructive instance research. However, this paper summarizes an approach with instance case analysis to improve customer loyalty via NPS data mining, which has extensive and practical significance for tele-companies. First, this paper finds some driven forces of customer loyalty, which are relative to customer consumption such as the call duration, the usage of data, ARPU, etc., by using some innovative reasoning-analysis based on IG(Information Gain) and xg-boost decision-making tree model, so the tele-companies can predict the role of individual customer and form daily monitoring on big data, which will save a lot of NPS survey cost. Second, this paper summarizes how customer group feature impacts the relationship between NPS and financial performance. Taking ARPU value as the performance goals, we divide the sample customers into 6 groups and summarize their characteristics based on k-means clustering, and give targeted suggestion of each group.
文摘Physical education teaching mode is a general teaching procedure that embodies certain teaching ideas and aims and organizesteaching by a series of teaching methods and means. Implementing the experiential teaching mode of college physical educationhas become an important part of the reform of College Physical Education in China. The university sports experiential teachingmode is an important form of teaching. It helps students to be independent and proactive in physical education. It is an importantway to cultivate innovative talents and improve the teaching effect of universities. Starting from the meaning and characteristics ofthe experiential teaching mode, this paper expounds the implementation significance of the current physical education teachingstatus and problems, and finally explores effective application strategies.
文摘Metal-organic frameworks(MOFs)are a new class of crystalline porous materials with periodic network structures,which are formed by self-assembly between metal ions and bridging organic linkers.Due to high surface areas,large pore volumes,and tunable surface properties,MOFs present promising application in gas storage and separation,drug delivery,heterogeneous catalysis,etc.Recently,MOFs are found to
基金This work was supported by the National Key Research and Development Program of China(2018YFD0900501)Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS(20603022022005)+6 种基金Shinan District Science and Technology Foundation(Qingdao)(2022-2-027-ZH)Central Public-interest Scientific Institution Basal Research Fund,CAFS(2020TD39)China Agriculture Research System(CARS-48)C.L.was supported by the Youth Innovation Team of Shandong Higher Education Institution(2021KJ064)the National Natural Science Foundation of China(32200004)W.S.was supported by the Academic Promotion Programme of Shandong First Medical University(2019QL006)E.C.H.was funded by a National Medical Health and Research Council(Australia)Investigator Grant(GNT2017197).
文摘Brine shrimp(Artemia)has existed on Earth for 400 million years and has major ecological importance in hypersaline ecosystems.As a crucial live food in aquaculture,brine shrimp cysts have become one of the most important aquatic products traded worldwide.However,our understanding of the biodiversity,prevalence and global connectedness of viruses in brine shrimp is still very limited.A total of 143 batches of brine shrimp(belonging to seven species)cysts were collected from six continents including 21 countries and more than 100 geographic locations worldwide during 1977–2019.In total,55 novel RNA viruses were identified,which could be assigned to 18 different viral families and related clades.Eleven viruses were dsRNA viruses,16 were+ssRNA viruses,and 28 were−ssRNA viruses.Phylogenetic analyses of the RNA-directed RNA polymerase(RdRp)showed that brine shrimp viruses were often grouped with viruses isolated from other invertebrates and fungi.Remarkably,most brine shrimp viruses were related to those from different hosts that might feed on brine shrimp or share the same ecological niche.A notable case was the novel brine shrimp noda-like virus 3,which shared 79.25%(RdRp)and 63.88%(capsid proteins)amino acid identity with covert mortality nodavirus(CMNV)that may cause losses in aquaculture.In addition,both virome composition and phylogenetic analyses revealed global connectedness in certain brine shrimp viruses,particularly among Asia and Northern America.This highlights the incredible species diversity of viruses in these ancient species and provides essential data for the prevalence of RNA viruses in the global aquaculture industry.More broadly,these findings provide novel insights into the previously unrecognized RNA virosphere in hypersaline ecosystems worldwide and demonstrate that human activity might have driven the global connectedness of brine shrimp viruses.
基金supported by the National Natural Science Foundation of China(No.41771283)"Longyun Program"of the College of Life Science and Technology of Huazhong Agricultural University。
文摘Toxic arsenic(As)and trace element selenium(Se)are transformed by microorganisms but their complex interactions in soil-plant systems have not been fully understood.An Asand Se-oxidizing bacterium,Agrobacterium sp.T3F4,was applied to a native seleniferous As-polluted soil to investigate As/Se uptake by the vegetable Brassica rapa L.and As-Se interaction as mediated by strain T3F4.The Se content in the aboveground plants was significantly enhanced by 34.1%,but the As content was significantly decreased by 20.5% in the T3F4-inoculated pot culture compared to the control(P<0.05).Similar result was shown in treatment with additional 5 mg/kg of Se(IV)in soil.In addition,the As contents in roots were significantly decreased by more than 35% under T3F4 or Se(IV)treatments(P<0.05).Analysis of As-Se-bacterium interaction in a soil simulation experiment showed that the bioavailability of Se significantly increased and As was immobilized with the addition of the T3F4strain(P<0.05).Furthermore,an As/Se co-exposure hydroponic experiment demonstrated that As uptake and accumulation in plants was reduced by increasing Se(IV)concentrations.The 50% growth inhibition concentration(IC50)values for As in plants were increased about one-fold and two-fold under co-exposure with 5 and 10μmol/L Se(IV),respectively.In conclusion,strain T3F4 improves Se uptake but decreases As uptake by plants via oxidation of As and Se,resulting in decrease of soil As bioavailability and As/Se competitive absorption by plants.This provides a potential bioremediation strategy for Se biofortification and As immobilization in As-polluted soil.
基金supported by grants from the Department of Science and Technology of the Fujian Province Project(Nos.2017I0004 and 2017Y9057)the Fujian provincial health technology project(No.2021ZD01005)+2 种基金the National Key Clinical Specialty Discipline Construction Program(No.2021-76)the Fujian Provincial Clinical Research Center for Hematological Malignancies(No.2020Y2006)the Fujian Provincial Key Clinical Specialty Discipline Construction Program.
文摘To the Editor:Upfront autologous stem cell transplantation(ASCT)has been an integral part of the management of young and eligible older patients with newly diagnosed multiple myeloma(NDMM)for over three decades.Tandem stem cell transplantation was defined as two planned sequential ASCTs within 3 to 6 months.More than two decades after its introduction,trials exploring the utility of tandem transplants have yielded mixed results.The role of tandem ASCT in multiple myeloma(MM)remains unclear and controversial.In the EMN02/HOVON95 study.
文摘Extracellular vesicles from highly metastatic tumor cells have been shown to mediate epithelial-mesenchymal transition(EMT)-related events in recipient cells.In endometrial epithelial cells,EMT processes are known to be involved in the development of adenomyosis.We aimed to investigate whether adenomyosis-derived extracellular vesicles(AMEVs)are able to induce an EMT process in endometrial epithelial cells.In this study,AMEVs were isolated from patients with adenomyosis and characterized by transmission electron microscopy,Western blot,and nanoparticle tracking.Primary endometrial epithelial cells(EECs)were derived from normal endometrium tissues from patients with leiomyoma and co-cultured with AMEVs in vitro.AMEV uptake was examined by fluorescence confocal microscopy.The invasion of EECs was confirmed by Transwell assay.Immunohistochemistry,Western blot,and qRT-PCR were performed on EECs to illustrate the expression levels of cytokeratin 19,E-cadherin,vimentin,and zinc finger E-boxbinding homeobox 1(ZEB1).The results indicated that the cellular fluorescence intensity gradually increased after 48 h of co-culture,but decreased after 72 h.After co-culturing with AMEVs for 72 h,EECs expressed significantly lower levels of cytokeratin 19 and E-cadherin,and significantly higher levels of vimentin and ZEB1.Together these results demonstrated that AMEVs induce an EMT process and enhance the invasion of EECs.These changes may contribute to the pathogenesis and progression of adenomyosis.
基金The Key Bio-safety Research and Development Technology Project(2021YFC1200200)The Key Scientific and Technology Project of Inner Mongolia Autonomous Region(2021ZD0006).
文摘Summary What is already known about this topic?There were a total of 4 and 3 human plague cases that occurred in the Inner Mongolia Autonomous Region in 2019 and 2020,respectively,with 1 and 2 deaths in 2019 and 2020 respectively,which indicated that plague still poses a significant threat to human health especially for farmers,shepherds,or residents living in native plague foci.What is added by this report?On August 14,2021,1 patient from the Otog Qi(County)in the Inner Mongolia sought treatment in Yinchuan City(the capital of Ningxia Hui Autonomous Region).
基金the China National Natural Science Foundation(Grant Nos.72104123,72004113,and 71790613).
文摘An emergency platform is an informatization support platform for disaster information perception,disaster situa-tional awareness,and emergency decision command.This is a key tool for ensuring the efficiency and effectiveness of emergency responses.Numerous large countries have conducted in-depth research on the key technologies of emergency platforms.Over the past 20 years,with the reform of emergency management mechanisms,China has developed two generations of emergency platform systems.This paper reviews the two generations of emergency platforms and summarizes their key technologies including multi-source-based monitoring and early warning,multi-hazard risk assessment,“scenario-response”-based decision support,synthetical forecasting based on in-cident chain,and emergency common operational picture(COP)for command and dispatch.Future research directions for the next generation emergency platforms are also proposed.
基金the support of the National Natural Science Foundation of China(Grant No.51979167,51179103)the Ministry of Industry and Information Technology(Grant No.[2018]473)+1 种基金the China Scholarship Council(Grant 201806230206)the Hainan Provincial Joint Project of Sanya Bay Science and Technology City(Grant No.520LH051).
文摘The linearity assumption is widely used when acquiring the hydrodynamic coefficients of a floating structure.However,the linear damping is frequently underestimated,especially for the natural frequency.To investigate the sloping seafloor effects on the damping terms of a single module of a semi-submersible Very Large Floating Structure(VLFS),this paper revisits the conventional formulation and further proposes the direct integration method for obtaining the linear and quadratic damping coefficients from free-decay tests.Numerical free-decay simulations of the single module over variable bathymetry are carried out by the CFD numerical tank.Corresponding model tests are also implemented to verify and validate against the numerical solutions.The effects of the sloping seafloor,as well as the water depth,on the hydrodynamic coefficients are investigated based on the validated CFD modeling.Both numerical and experimental results indicate that the acquisition of the linear and quadratic damping coefficients is sensitive to the data-processing and identification approaches.For the case studied in present paper,the identification errors introduced by the conventional method are 1.5%while they are 0.5%using the direct integration method.The quadratic damping coefficient for heave mode decreases about 10.4%when the sloping angle increases from 0 to 6 deg.
文摘The coronavirus disease 2019(COVID-19)pandemic started in early 2020.At the beginning of February,a public welfare activity in epidemic data visualization,jointly launched by China Computer Federation(CCF)(CCF)CAD&CG Technical Committee,Alibaba Cloud Tianchi(Alibaba Cloud Tianch),JiqiZhixin(JiqiZhixin),Alibaba Cloud DataV(Alibaba Cloud DataV),and DataWhale(DataWhale),was launched with the theme‘‘Fighting the Epidemic with One Mind and Talents like Tianchi.’’Developers in general are expected to focus on several demand scenarios,such as epidemic situation display,epidemic popular science,trend prediction,material-supply situation,and rework and return situation of employees from all sectors and areas,to discover the relationship between complex heterogeneous multi-source data,develop various upbeat works and present useful information to the public in a coherent manner.The entry works take the form of data visualization and are divided into two categories:popular science publicity and application scenarios.The popular science publicity category includes works for the public,focused on epidemic situation display,epidemic popular science publicity,epidemic prevention and control,and others.The application scenario category consists of the works of frontline officers,which can provide anti-epidemic workers with effective data tools for efficient and intuitive epidemic analysis;offer reliable,understandable,and easily transmitted information for disease prevention;and assist governments,enterprises,and institutions in the fight against COVID-19.
