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铁死亡诱导剂Erastin下调ACSL4抑制肝癌细胞体外增殖
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作者 赵培培 周志刚 +3 位作者 杨媛媛 黄树升 涂逸轩 涂剑 《南方医科大学学报》 CAS CSCD 北大核心 2024年第11期2131-2136,共6页
目的分析酯酰辅酶A合成酶长链家族成员4(ACSL4)在肝癌中的表达,探究铁死亡调控ACSL4对癌细胞增殖能力的影响。方法收集肝癌和癌旁正常肝组织临床样本,HE染色病理学鉴定后,微量法检测丙二醛(MDA)含量,RT-qPCR检测ACSL4与增殖细胞核抗原(P... 目的分析酯酰辅酶A合成酶长链家族成员4(ACSL4)在肝癌中的表达,探究铁死亡调控ACSL4对癌细胞增殖能力的影响。方法收集肝癌和癌旁正常肝组织临床样本,HE染色病理学鉴定后,微量法检测丙二醛(MDA)含量,RT-qPCR检测ACSL4与增殖细胞核抗原(PCNA)的mRNA表达,Western blotting检测ACSL4与PCNA的蛋白表达。体外培养Huh-7人肝癌细胞,先分为3组:即铁死亡诱导剂Erastin组、抑制剂Fer-1组、以及Erastin与Fer-1联合作用组;其中Erastin或Fer-1组均包含0、20、40、60、80、100μmol/L共6个浓度,然后采用筛选的Erastin浓度80μmol/L+(0、30、60、90μmol/L)Fer-1,筛选Fer-1浓度后再分为3组:对照组、80μmol/L Erastin单独处理组、80μmol/L Erastin+60μmol/L Fer-1联合处理组,均作用48 h。干预ACSL4、PCNA的表达后,平板克隆实验检测细胞增殖能力的改变,微量法检测MDA含量的变化。结果相较于癌旁正常肝组织,肝癌组织中MDA含量降低(P<0.01),ACSL4、PCNA的mRNA和蛋白表达均显著增强(P<0.05);Erastin可抑制ACSL4、PCNA的mRNA和蛋白表达(P<0.01),并抑制细胞增殖(P<0.001)、上调MDA含量(P<0.01);单独使用Fer-1对细胞存活率无影响;但加入Erastin后再应用Fer-1,则可逆转Erastin对ACSL4、PCNA表达(P<0.05),细胞增殖能力的抑制(P<0.001),MDA含量的上调(P<0.05)。结论ACSL4在肝癌中表达增强,Erastin可提高MDA含量、下调ACSL4表达,诱导肝癌细胞铁死亡,抑制癌细胞增殖;Fer-1则可逆转Erastin的上述作用。 展开更多
关键词 肝癌 细胞增殖 Erastin ACSL4 铁死亡
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Land use and land cover classification using Chinese GF-2 multispectral data in a region of the North China Plain 被引量:3
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作者 Kun JIA Jingcan LIU +5 位作者 yixuan tu Qiangzi LI Zhiwei SUN Xiangqin WEI Yunjun YAO Xiaotong ZHANG 《Frontiers of Earth Science》 SCIE CAS CSCD 2019年第2期327-335,共9页
The newly launched GF-2 satellite is now the most advanced civil satellite in China to collect high spatial resolution remote sensing data.This study investigated the capability and strategy of GF?2 multispectral data... The newly launched GF-2 satellite is now the most advanced civil satellite in China to collect high spatial resolution remote sensing data.This study investigated the capability and strategy of GF?2 multispectral data for land use and land cover (LULC) classification in a region of the North China Plain.The pixel-based and object-based classifications using maximum likelihood (MLC) and support vector machine (SVM) classifiers were evaluated to determine the classification strategy that was suitable for GF?2 multispectral data.The validation results indicated that GF-2 multispectral data achieved satisfactory LULC classification performance,and object-based classification using the SVM classifier achieved the best classification accuracy with an overall classification accuracy of 94.33% and kappa coefficient of 0.911.Therefore,considering the LULC classification performance and data characteristics,GF-2 satellite data could serve as a valuable and reliable high-resolution data source for land surface monitoring.Future works should focus on improving LULC classification accuracy by exploring more classification features and exploring the potential applications of GF-2 data in related applications. 展开更多
关键词 LAND use and LAND COVER CLASSIFICATION GF-2 NORTH China PLAIN MULTISPECTRAL data
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Fractional vegetation cover estimation in heterogeneous areas by combining a radiative transfer model and a dynamic vegetation model 被引量:1
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作者 yixuan tu Kun Jia +3 位作者 Shunlin Liang Xiangqin Wei Yunjun Yao Xiaotong Zhang 《International Journal of Digital Earth》 SCIE 2020年第4期487-503,共17页
A fractional vegetation cover(FVC)estimation method incorporating a vegetation growth model and a radiative transfer model was previously developed,which was suitable for FVC estimation in homogeneous areas because th... A fractional vegetation cover(FVC)estimation method incorporating a vegetation growth model and a radiative transfer model was previously developed,which was suitable for FVC estimation in homogeneous areas because the finer-resolution pixels corresponding to one coarseresolution FVC pixel were all assumed to have the same vegetation growth model.However,this assumption does not hold over heterogeneous areas,meaning that the method cannot be applied to large regions.Therefore,this study proposes a finer spatial resolution FVC estimation method applicable to heterogeneous areas using Landsat 8 Operational Land Imager reflectance data and Global LAnd Surface Satellite(GLASS)FVC product.The FVC product was first decomposed according to the normalized difference vegetation index from the Landsat 8 OLI data.Then,independent dynamic vegetation models were built for each finer-resolution pixel.Finally,the dynamic vegetation model and a radiative transfer model were combined to estimate FVC at the Landsat 8 scale.Validation results indicated that the proposed method(R^(2)=0.7757,RMSE=0.0881)performed better than either the previous method(R^(2)=0.7038,RMSE=0.1125)or a commonly used method involving look-up table inversions of the PROSAIL model(R^(2)=0.7457,RMSE=0.1249). 展开更多
关键词 Dynamic Bayesian network fractional vegetation cover global land surface satellite radiative transfer model dynamic vegetation model
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