Power&signal multiplex transmission(P&SMT)is a technique that uses power electronic circuits for communication signal transmission.In this paper,a three-phase cascaded multilevel inverter-based P&S MT syst...Power&signal multiplex transmission(P&SMT)is a technique that uses power electronic circuits for communication signal transmission.In this paper,a three-phase cascaded multilevel inverter-based P&S MT system is proposed.The proposed method can transmit communication signals without using a Controller Area Network bus,thereby reducing the wiring cost of the conventional electric vehicle(EV)communication system.The designed system can achieve motor speed regulation and battery balance discharging for EVs.With the combined pulse width modulation scheme and frequency shift keying method,both power and communication signals are transmitted successfully in a simulation model implemented in Matlab/Simulink.By evaluating the bit error rate of the transmitted signal,the maximum signal rate of the proposed system is determined as 600 bit/s.展开更多
Full-duplex(FD)has been recognized as a promising technology for future 5G networks to improve the spectrum efficiency.However,the biggest practical impediments of realizing full-duplex communications are the presence...Full-duplex(FD)has been recognized as a promising technology for future 5G networks to improve the spectrum efficiency.However,the biggest practical impediments of realizing full-duplex communications are the presence of self-interference,especially in complex cellular networks.With the current development of self-interference cancellation techniques,full-duplex has been considered to be more suitable for device-to-device(D2D)and small cell communications which have small transmission range and low transmit power.In this paper,we consider the full-duplex D2D communications in multi-tier wireless networks and present an analytical model which jointly considers mode selection,resource allocation,and power control.Specifically,we consider a distance based mode selection scheme.The performance analysis of different D2D communications modes are performed based on stochastic geometry,and tractable analytical solutions are obtained.Then we investigate the optimal resource partitions between dedicated D2D mode and cellular mode.Numerical results validate the theoretical anlaysis and indicate that with appropriate proportions of users operated in different transmission modes and optimal partitioning of spectrum,the performance gain of FD-D2D communication can be achieved.展开更多
With the emergence of ambient sensing technologies which combine mobile crowdsensing and Internet of Things,large amount of people-centric data can be obtained and utilized to build people-centric services.Note that t...With the emergence of ambient sensing technologies which combine mobile crowdsensing and Internet of Things,large amount of people-centric data can be obtained and utilized to build people-centric services.Note that the service quality is highly related to the privacy level of the data.In this paper,we investigate the problem of privacy-aware service subscription in people-centric sensing.An efficient resource allocation framework using a combinatorial auction(CA)model is provided.Specifically,the resource allocation problem that maximizes the social welfare in view of varying requirements of multiple users is formulated,and it is solved by a proposed computationally tractable solution algorithm.Furthermore,the prices of allocated resources that winners need to pay are figured out by a designed scheme.Numerical results demonstrate the effectiveness of the proposed scheme.展开更多
Alveolar bone regeneration has been strongly linked to macrophage polarization.M1 macrophages aggravate alveolar bone loss,whereas M2 macrophages reverse this process.Berberine(BBR),a natural alkaloid isolated and ref...Alveolar bone regeneration has been strongly linked to macrophage polarization.M1 macrophages aggravate alveolar bone loss,whereas M2 macrophages reverse this process.Berberine(BBR),a natural alkaloid isolated and refined from Chinese medicinal plants,has shown therapeutic effects in treating metabolic disorders.In this study,we first discovered that culture supernatant(CS)collected from BBR-treated human bone marrow mesenchymal stem cells(HBMSCs)ameliorated periodontal alveolar bone loss.CS from the BBR-treated HBMSCs contained bioactive materials that suppressed the M1 polarization and induced the M2 polarization of macrophages in vivo and in vitro.To clarify the underlying mechanism,the bioactive materials were applied to different animal models.We discovered macrophage colony-stimulating factor(M-CSF),which regulates macrophage polarization and promotes bone formation,a key macromolecule in the CS.Injection of pure M-CSF attenuated experimental periodontal alveolar bone loss in rats.Colony-stimulating factor 1 receptor(CSF1R)inhibitor or anti-human M-CSF(M-CSF neutralizing antibody,Nab)abolished the therapeutic effects of the CS of BBR-treated HBMSCs.Moreover,AKT phosphorylation in macrophages was activated by the CS,and the AKT activator reversed the negative effect of the CSF1R inhibitor or Nab.These results suggest that the CS of BBR-treated HBMSCs modulates macrophage polarization via the M-CSF/AKT axis.Further studies also showed that CS of BBR-treated HBMSCs accelerated bone formation and M2 polarization in rat teeth extraction sockets.Overall,our findings established an essential role of BBR-treated HBMSCs CS and this might be the first report to show that the products of BBR-treated HBMSCs have active effects on alveolar bone regeneration.展开更多
A wide spectrum of metabolites(mainly,the three major nutrients and their derivatives)can be sensed by specific sensors,then trigger a series of signal transduction pathways and affect the expression levels of genes i...A wide spectrum of metabolites(mainly,the three major nutrients and their derivatives)can be sensed by specific sensors,then trigger a series of signal transduction pathways and affect the expression levels of genes in epigenetics,which is called metabolite sensing.Life body regulates metabolism,immunity,and inflammation by metabolite sensing,coordinating the pathophysiology of the host to achieve balance with the external environment.Metabolic reprogramming in cancers cause different phenotypic characteristics of cancer cell from normal cell,including cell proliferation,migration,invasion,angiogenesis,etc.Metabolic disorders in cancer cells further create a microenvironment including many kinds of oncometabolites that are conducive to the growth of cancer,thus forming a vicious circle.At the same time,exogenous metabolites can also affect the biological behavior of tumors.Here,we discuss the metabolite sensing mechanisms of the three major nutrients and their derivatives,as well as their abnormalities in the development of various cancers,and discuss the potential therapeutic targets based on metabolite-sensing signaling pathways to prevent the progression of cancer.展开更多
Flow field data generated by ocean models are important for simulating ocean currents and circulation patterns,which are essential components in digital Earth construction.To evaluate the accuracy of model-simulatedfl...Flow field data generated by ocean models are important for simulating ocean currents and circulation patterns,which are essential components in digital Earth construction.To evaluate the accuracy of model-simulatedflow felds,Array for Real-timeGeostrophic Oceanography(Argo)float observations can be considered benchmarks.In this study,a novel method for comparing Argo profiles with 3-dimensional trajectories obtained by simulating Argo floats in Hybrid Coordinate Ocean Model(HYCOM)-provided flow fields was proposed.Surface and subsurface trajectories were calculated,and their spatial matching characteristics were analyzed.The results demonstrated that(1)the HYCOM surface and subsurface flow felds generally conform to the basic characteristics and trends of ocean currents;(2)the HYCOM sea surface current field error pattern exhibits a symmetrical distribution centered on the equator in the Northern and Southern Hemispheres and increases with increasing latitude;and(3)the HYCOM subsurface flow field exhibits regional differences,with the largest differences in the Gulf Stream,North Atlantic Warm Current,and Westerly Wind Drift region.Through analysis of the disparities between HYCOM and Argo data,the effectiveness of using model simulation data can be enhanced,and the accuracy and dependability of ocean models can be improved.展开更多
Dissolution of lithium cobalt oxide(LCO)is the key step for the recovery of valuable metals(e.g.,Co and Li)from spent LCO-based lithium-ion batteries(LIBs).However,the dissolution process of LCO either needs toxic sol...Dissolution of lithium cobalt oxide(LCO)is the key step for the recovery of valuable metals(e.g.,Co and Li)from spent LCO-based lithium-ion batteries(LIBs).However,the dissolution process of LCO either needs toxic solvents,and high temperature,or shows low efficiency.Deep eutectic solvents(DESs)are potential green solvents to dissolve LCO.Here,DESs with polyethylene glycol(PEG)as hydrogen bond acceptor and ascorbic acid(AA)as hydrogen bond donor are found to dissolve LCO with 84.2%Co leaching efficiency at 80℃ and 72 h,which is higher than that from the reported references by common DESs.Furthermore,both DESs components(i.e.,PEG and AA)are cheap,biodegradable,and biocompatible.AA could be easily and abundantly extracted from natural fruits or vegetables.It provides a new guide for the green,mild,and efficient dissolution of LCO aiming at sustainable recovery of spent LIBs.展开更多
X-ray absorption spectroscopy(XAS)is a well-established method for in-depth characterization of electronic structure.In practice hundreds of energy-points should be sampled during the measurements,and most of them are...X-ray absorption spectroscopy(XAS)is a well-established method for in-depth characterization of electronic structure.In practice hundreds of energy-points should be sampled during the measurements,and most of them are redundant.Additionally,it is also tedious to estimate reasonable parameters in the atomic Hamiltonians for mechanistic understanding.We implement an Adversarial Bayesian optimization(ABO)algorithm comprising two coupled BOs to automatically fit the many-body model Hamiltonians and to sample effectively based on active learning(AL).Taking NiO as an example,we find that less than 30 sampling points are sufficient to recover the complete XAS with the corresponding crystal field and charge transfer models,which can be selected based on intuitive hypothesis learning.Further applications on the experimental XAS spectra reveal that less than 80 sampling points give reasonable XAS and reliable atomic model parameters.Our ABO algorithm has a great potential for future applications on automated physics-driven XAS analysis and AL sampling.展开更多
We have developed a protein array system,named"Phospho-Totum",which reproduces the phosphorylation state of a sample on the array.The protein array contains 1471 proteins from 273 known signaling pathways.Ac...We have developed a protein array system,named"Phospho-Totum",which reproduces the phosphorylation state of a sample on the array.The protein array contains 1471 proteins from 273 known signaling pathways.According to the activation degrees of tyrosine kinases in the sample,the corresponding groups of substrate proteins on the array are phosphorylated under the same conditions.In addition to measuring the phosphorylation levels of the 1471 substrates,we have developed and performed the artificial intelligence-assisted tools to further characterize the phosphorylation state and estimate pathway activation,tyrosine kinase activation,and a list of kinase inhibitors that produce phosphorylation states similar to that of the sample.The Phospho-Totum system,which seamlessly links and interrogates the measurements and analyses,has the potential to not only elucidate pathophysiological mechanisms in diseases by reproducing the phosphorylation state of samples,but also be useful for drug discovery,particularly for screening targeted kinases for potential drug kinase inhibitors.展开更多
Background:Burn shock caused by vascular leakage is one of the main causes of high mortality in severe burn injury.However,the pathophysiological mechanism of vascular leakage is still unclear.The purpose of this stud...Background:Burn shock caused by vascular leakage is one of the main causes of high mortality in severe burn injury.However,the pathophysiological mechanism of vascular leakage is still unclear.The purpose of this study was to explore the molecular mechanism of vascular leakage in the early stage of severe burn and provide a new target for the treatment of severe burns.Methods:Neutrophils were isolated from human peripheral blood by magnetic beads sorting.ELISA was used to detect neutrophil-derived granule proteins and glycocalyx injury products in plasma.The vascular leakage and neutrophil movement were assessed by in vivo laser confocal imaging in mice,and high-quality video were provided.Adhesion-related molecules were investigated by qRT-PCR.The damage to glycocalyx of mice vascular endothelial cellswas observed by transmission electron microscope and scanning electron microscope.Proteomic analysis,flow cytometry and immunofluorescence were used to further study the relationship between human peripheral blood neutrophil-derived hypochlorite(HOCl)and CD44 of human vascular endothelial cells.Results:In this study,we found that rapidly increasing activated neutrophils secrete heparin binding protein(HBP)andmyeloperoxidase(MPO)after severe burn injury.Increased HBP triggers vascular leakage with synergy of MPO,results in systemic edema and burn shock.Furthermore,we found that the MPO catalytic product HOCl but not MPO triggers CD44 extracellular domain shedding from vascular endothelial cells to damage the glycocalyx.Damage to the glycocalyx results in firm adhesion of neutrophils and increases vascular leakage.However,MPO inhibitors partially protect the glycocalyx of vascular endothelial cells.The combination of HBP and MPO inhibitors markedly reduces vascular leakage and systemic edema in the early stage of severe burns.Conclusions:Taken together,these data reveal that neutrophil-derived HBP and MPO play an important synergies role in triggering vascular leakage at the early stage of severe burns.Targeted intervention in these two biomolecules may introduce new strategies for helping to reduce large amount of fluid loss and subsequent burn shock.展开更多
Autonomous materials discovery with desired properties is one of the ultimate goals for materials science,and the current studies have been focusing mostly on high-throughput screening based on density functional theo...Autonomous materials discovery with desired properties is one of the ultimate goals for materials science,and the current studies have been focusing mostly on high-throughput screening based on density functional theory calculations and forward modeling of physical properties using machine learning.Applying the deep learning techniques,we have developed a generative model,which can predict distinct stable crystal structures by optimizing the formation energy in the latent space.It is demonstrated that the optimization of physical properties can be integrated into the generative model as on-top screening or backward propagator,both with their own advantages.Applying the generative models on the binary Bi-Se system reveals that distinct crystal structures can be obtained covering the whole composition range,and the phases on the convex hull can be reproduced after the generated structures are fully relaxed to the equilibrium.The method can be extended to multicomponent systems for multi-objective optimization,which paves the way to achieve the inverse design of materials with optimal properties.展开更多
Despite being a common therapy for hepatocellular carcinoma(HCC),insufficient thermal ablation can leave behind tumor residues that can cause recurrence.This is believed to augment M2 inflammatory macrophages that usu...Despite being a common therapy for hepatocellular carcinoma(HCC),insufficient thermal ablation can leave behind tumor residues that can cause recurrence.This is believed to augment M2 inflammatory macrophages that usually play a pro-tumorigenic role.To address this problem,we designed D-mannose-chelated iron oxide nanoparticles(man-IONPs) to polarize M2-like macrophages into the antitumor Ml phenotype.In vitro and in vivo experiments demonstrated that man-IONPs specifically targeted M2-like macrophages and accumulated in peri-ablation zones after macrophage infiltration was augmented under insufficient microwave ablation(MWA).The nanoparticles simultaneously induced polarization of pro-tumorigenic M2 macrophages into antitumor M1 phenotypes,enabling the transformation of the immunosuppressive microenvironment into an immunoactivating one.Post-MWA macrophage polarization exerted robust inhibitory effects on HCC progression in a well-established orthotopic liver cancer mouse model.Thus,combining thermal ablation with man-IONPs can salvage residual tumors after insufficient MWA.These results have strong potential for clinical translation.展开更多
Dear Editor,Porcine deltacoronavirus(PDCoV)is a novel discovered swine enteric coronavirus which can cause diarrhea and dehydration in pigs,particularly in neonatal piglets(Jung et al.,2016).At present,there are no co...Dear Editor,Porcine deltacoronavirus(PDCoV)is a novel discovered swine enteric coronavirus which can cause diarrhea and dehydration in pigs,particularly in neonatal piglets(Jung et al.,2016).At present,there are no commercial vaccines available for PDCo V.To control PDCoV transmission and perform antiviral therapy efficiently,a rapid and accurate diagnostic method to detect PDCo V is needed.展开更多
A novel 1 kHz single-frequency, Q-switched Er-doped yttrium aluminum garnet(Er:YAG) laser pumped by a 1470 nm laser diode is demonstrated. The 500 ns, 5.52 mJ single-frequency, diffraction-limited pulses are obtained ...A novel 1 kHz single-frequency, Q-switched Er-doped yttrium aluminum garnet(Er:YAG) laser pumped by a 1470 nm laser diode is demonstrated. The 500 ns, 5.52 mJ single-frequency, diffraction-limited pulses are obtained by using a ‘ramp-fire' injection-seeding technique and an optical feedback architecture. The full width at half-maximum of the pulse spectrum is measured to be 1.47 MHz by using the heterodyne technique. The beam quality M2 factors are measured to be 1.18 and 1.24 in the x and y directions, respectively.展开更多
The drug resistance of chemotherapy is a major challenge to overcome for antineoplastic agents and the reverse of drug resistant is essential for cancer therapy. Herein, we developed a drug delivery system which can s...The drug resistance of chemotherapy is a major challenge to overcome for antineoplastic agents and the reverse of drug resistant is essential for cancer therapy. Herein, we developed a drug delivery system which can simultaneously detect/reverse the drug resistance and perform synergetic treatment of cancer. In this work, we integrated cyanine5(Cy5) modified mi RNA(let-7 i)(Cy5-mi RNA) and platinum onto nano-graphene oxide(NGO)(30-50 nm) platform to achieve simultaneously detection/reversion of drug resistance and synergetic treatment of cisplatin resistant SKOV3 cells(SKOV3 DDP cells). The Cy5-mi RNA adsorbed on NGO could selectively bind the drug resistance related m RNA follow by suppress the expression of drug resistance m RNA, and the binding simultaneously induced the release of Cy5-mi RNA from the NGO, thus the fluorescence signal of Cy5 recovered and could be used for drug resistance monitoring.Moreover, the mi RNA suppressed the Cyclin D1 protein expressions thus reversed the drug resistance. The loaded platinum(Ⅳ)(Pt(Ⅳ)) was converted to the therapeutic platinum(Ⅱ)(Pt(Ⅱ)) in both tumor acidic and reductive environment responsive behavior. NGO furtherly performed photothermal therapy under near infrared(NIR) laser irradiation and enhanced the therapeutic effect. All in all, this nanoplatform realized detection/reversion of the drug resistance as well as synergetic chemical-photothermal treatment of ovarian cancer cells, which holds great promise in the treatment of drug resistant cancer cells.展开更多
Geographic simulation models can be used to explore and better understand the geographical environment. Recent advances in geographic and socio-environmental research have led to a dramatic increase in the number of m...Geographic simulation models can be used to explore and better understand the geographical environment. Recent advances in geographic and socio-environmental research have led to a dramatic increase in the number of models used for this purpose. Some model repositories provide opportunities for users to explore and apply models,but few provide a general evaluation method for assessing the applicability and recognition of models. In this study,an academic impact evaluation method for models is proposed. Five indices are designed based on their pertinence. The analytical hierarchy process is used to calculate the index weights,and the academic impacts of models are quantified with the weighted sum method. The time range is controlled to evaluate the life-term and annual academic impacts of the models. Some models that met the evaluation criteria from different domains are then evaluated. The results show that the academic impact of a model can be quantified with the proposed method,and the major research areas that models impact are identified.展开更多
文摘Power&signal multiplex transmission(P&SMT)is a technique that uses power electronic circuits for communication signal transmission.In this paper,a three-phase cascaded multilevel inverter-based P&S MT system is proposed.The proposed method can transmit communication signals without using a Controller Area Network bus,thereby reducing the wiring cost of the conventional electric vehicle(EV)communication system.The designed system can achieve motor speed regulation and battery balance discharging for EVs.With the combined pulse width modulation scheme and frequency shift keying method,both power and communication signals are transmitted successfully in a simulation model implemented in Matlab/Simulink.By evaluating the bit error rate of the transmitted signal,the maximum signal rate of the proposed system is determined as 600 bit/s.
基金This work was supported by National Natural Science Foundation of China.The grant number is 61672283.
文摘Full-duplex(FD)has been recognized as a promising technology for future 5G networks to improve the spectrum efficiency.However,the biggest practical impediments of realizing full-duplex communications are the presence of self-interference,especially in complex cellular networks.With the current development of self-interference cancellation techniques,full-duplex has been considered to be more suitable for device-to-device(D2D)and small cell communications which have small transmission range and low transmit power.In this paper,we consider the full-duplex D2D communications in multi-tier wireless networks and present an analytical model which jointly considers mode selection,resource allocation,and power control.Specifically,we consider a distance based mode selection scheme.The performance analysis of different D2D communications modes are performed based on stochastic geometry,and tractable analytical solutions are obtained.Then we investigate the optimal resource partitions between dedicated D2D mode and cellular mode.Numerical results validate the theoretical anlaysis and indicate that with appropriate proportions of users operated in different transmission modes and optimal partitioning of spectrum,the performance gain of FD-D2D communication can be achieved.
基金This work was partially supported by National Natural Science Foundation of China under Grant No.61801167Natural Science Foundation of Jiangsu Province of China under Grant No.BK20160874.
文摘With the emergence of ambient sensing technologies which combine mobile crowdsensing and Internet of Things,large amount of people-centric data can be obtained and utilized to build people-centric services.Note that the service quality is highly related to the privacy level of the data.In this paper,we investigate the problem of privacy-aware service subscription in people-centric sensing.An efficient resource allocation framework using a combinatorial auction(CA)model is provided.Specifically,the resource allocation problem that maximizes the social welfare in view of varying requirements of multiple users is formulated,and it is solved by a proposed computationally tractable solution algorithm.Furthermore,the prices of allocated resources that winners need to pay are figured out by a designed scheme.Numerical results demonstrate the effectiveness of the proposed scheme.
基金supported by the CAMS Innovation Foundation for Medical Sciences(2016-I2M1-011)A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(2018-87)+1 种基金Jiangsu Province Capability Improvement Project through Science,Technology and Education-Jiangsu Provincial Research Hospital Cultivation Unit(YJXYYJSDW4)Jiangsu Provincial Medical Innovation Center(CXZX202227)。
文摘Alveolar bone regeneration has been strongly linked to macrophage polarization.M1 macrophages aggravate alveolar bone loss,whereas M2 macrophages reverse this process.Berberine(BBR),a natural alkaloid isolated and refined from Chinese medicinal plants,has shown therapeutic effects in treating metabolic disorders.In this study,we first discovered that culture supernatant(CS)collected from BBR-treated human bone marrow mesenchymal stem cells(HBMSCs)ameliorated periodontal alveolar bone loss.CS from the BBR-treated HBMSCs contained bioactive materials that suppressed the M1 polarization and induced the M2 polarization of macrophages in vivo and in vitro.To clarify the underlying mechanism,the bioactive materials were applied to different animal models.We discovered macrophage colony-stimulating factor(M-CSF),which regulates macrophage polarization and promotes bone formation,a key macromolecule in the CS.Injection of pure M-CSF attenuated experimental periodontal alveolar bone loss in rats.Colony-stimulating factor 1 receptor(CSF1R)inhibitor or anti-human M-CSF(M-CSF neutralizing antibody,Nab)abolished the therapeutic effects of the CS of BBR-treated HBMSCs.Moreover,AKT phosphorylation in macrophages was activated by the CS,and the AKT activator reversed the negative effect of the CSF1R inhibitor or Nab.These results suggest that the CS of BBR-treated HBMSCs modulates macrophage polarization via the M-CSF/AKT axis.Further studies also showed that CS of BBR-treated HBMSCs accelerated bone formation and M2 polarization in rat teeth extraction sockets.Overall,our findings established an essential role of BBR-treated HBMSCs CS and this might be the first report to show that the products of BBR-treated HBMSCs have active effects on alveolar bone regeneration.
基金supported by the National Natural Science Foundation of China[82072594(Y.T.),81874139,82073097(S.L.),81772496,82073136(D.X.)]Hunan Provincial Key Area R&D Programs[2019SK2253,2021SK2013,Y.T.]the Central South University Research Programme of Advanced Interdisciplinary Studies[2023QYJC030(Y.T.)].
文摘A wide spectrum of metabolites(mainly,the three major nutrients and their derivatives)can be sensed by specific sensors,then trigger a series of signal transduction pathways and affect the expression levels of genes in epigenetics,which is called metabolite sensing.Life body regulates metabolism,immunity,and inflammation by metabolite sensing,coordinating the pathophysiology of the host to achieve balance with the external environment.Metabolic reprogramming in cancers cause different phenotypic characteristics of cancer cell from normal cell,including cell proliferation,migration,invasion,angiogenesis,etc.Metabolic disorders in cancer cells further create a microenvironment including many kinds of oncometabolites that are conducive to the growth of cancer,thus forming a vicious circle.At the same time,exogenous metabolites can also affect the biological behavior of tumors.Here,we discuss the metabolite sensing mechanisms of the three major nutrients and their derivatives,as well as their abnormalities in the development of various cancers,and discuss the potential therapeutic targets based on metabolite-sensing signaling pathways to prevent the progression of cancer.
基金supported by National Natural Science Foundation of China[grant nos:41930648,42171406 and 42171465]National Key Research and Development Program of China[grant no:2021YFB3900901].
文摘Flow field data generated by ocean models are important for simulating ocean currents and circulation patterns,which are essential components in digital Earth construction.To evaluate the accuracy of model-simulatedflow felds,Array for Real-timeGeostrophic Oceanography(Argo)float observations can be considered benchmarks.In this study,a novel method for comparing Argo profiles with 3-dimensional trajectories obtained by simulating Argo floats in Hybrid Coordinate Ocean Model(HYCOM)-provided flow fields was proposed.Surface and subsurface trajectories were calculated,and their spatial matching characteristics were analyzed.The results demonstrated that(1)the HYCOM surface and subsurface flow felds generally conform to the basic characteristics and trends of ocean currents;(2)the HYCOM sea surface current field error pattern exhibits a symmetrical distribution centered on the equator in the Northern and Southern Hemispheres and increases with increasing latitude;and(3)the HYCOM subsurface flow field exhibits regional differences,with the largest differences in the Gulf Stream,North Atlantic Warm Current,and Westerly Wind Drift region.Through analysis of the disparities between HYCOM and Argo data,the effectiveness of using model simulation data can be enhanced,and the accuracy and dependability of ocean models can be improved.
基金This work was supported by the National Natural Science Foundation of China(22103030)Open Fund of Hebei Biomass Carbon Materials and Application Technology Innovation Center(SG2021003)Special Project for the Cultivation of Scientific and Technological Innovation Ability of College and Middle School Students in Hebei Province(22E50480D).
文摘Dissolution of lithium cobalt oxide(LCO)is the key step for the recovery of valuable metals(e.g.,Co and Li)from spent LCO-based lithium-ion batteries(LIBs).However,the dissolution process of LCO either needs toxic solvents,and high temperature,or shows low efficiency.Deep eutectic solvents(DESs)are potential green solvents to dissolve LCO.Here,DESs with polyethylene glycol(PEG)as hydrogen bond acceptor and ascorbic acid(AA)as hydrogen bond donor are found to dissolve LCO with 84.2%Co leaching efficiency at 80℃ and 72 h,which is higher than that from the reported references by common DESs.Furthermore,both DESs components(i.e.,PEG and AA)are cheap,biodegradable,and biocompatible.AA could be easily and abundantly extracted from natural fruits or vegetables.It provides a new guide for the green,mild,and efficient dissolution of LCO aiming at sustainable recovery of spent LIBs.
基金This work was also supported by the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)-Project-ID 405553726-TRR 270.We also acknowledge support by the Deutsche Forschungsgemeinschaft(DFG-German Research Foundation)and the Open Access Publishing Fund of Technical University of Darmstadt.
文摘X-ray absorption spectroscopy(XAS)is a well-established method for in-depth characterization of electronic structure.In practice hundreds of energy-points should be sampled during the measurements,and most of them are redundant.Additionally,it is also tedious to estimate reasonable parameters in the atomic Hamiltonians for mechanistic understanding.We implement an Adversarial Bayesian optimization(ABO)algorithm comprising two coupled BOs to automatically fit the many-body model Hamiltonians and to sample effectively based on active learning(AL).Taking NiO as an example,we find that less than 30 sampling points are sufficient to recover the complete XAS with the corresponding crystal field and charge transfer models,which can be selected based on intuitive hypothesis learning.Further applications on the experimental XAS spectra reveal that less than 80 sampling points give reasonable XAS and reliable atomic model parameters.Our ABO algorithm has a great potential for future applications on automated physics-driven XAS analysis and AL sampling.
基金supported by the State Key Program of National Natural Science Foundation of China(Grant No.82230114 to F.H.)the National Key Research and Development Program of China(Grant No.2022YFE0104800 to F.H.).
文摘We have developed a protein array system,named"Phospho-Totum",which reproduces the phosphorylation state of a sample on the array.The protein array contains 1471 proteins from 273 known signaling pathways.According to the activation degrees of tyrosine kinases in the sample,the corresponding groups of substrate proteins on the array are phosphorylated under the same conditions.In addition to measuring the phosphorylation levels of the 1471 substrates,we have developed and performed the artificial intelligence-assisted tools to further characterize the phosphorylation state and estimate pathway activation,tyrosine kinase activation,and a list of kinase inhibitors that produce phosphorylation states similar to that of the sample.The Phospho-Totum system,which seamlessly links and interrogates the measurements and analyses,has the potential to not only elucidate pathophysiological mechanisms in diseases by reproducing the phosphorylation state of samples,but also be useful for drug discovery,particularly for screening targeted kinases for potential drug kinase inhibitors.
基金supported by the National Natural Science Founda-tion of China,No.82072217,81772135the Jiangsu Natural Science Foundation,No.BK20201178。
文摘Background:Burn shock caused by vascular leakage is one of the main causes of high mortality in severe burn injury.However,the pathophysiological mechanism of vascular leakage is still unclear.The purpose of this study was to explore the molecular mechanism of vascular leakage in the early stage of severe burn and provide a new target for the treatment of severe burns.Methods:Neutrophils were isolated from human peripheral blood by magnetic beads sorting.ELISA was used to detect neutrophil-derived granule proteins and glycocalyx injury products in plasma.The vascular leakage and neutrophil movement were assessed by in vivo laser confocal imaging in mice,and high-quality video were provided.Adhesion-related molecules were investigated by qRT-PCR.The damage to glycocalyx of mice vascular endothelial cellswas observed by transmission electron microscope and scanning electron microscope.Proteomic analysis,flow cytometry and immunofluorescence were used to further study the relationship between human peripheral blood neutrophil-derived hypochlorite(HOCl)and CD44 of human vascular endothelial cells.Results:In this study,we found that rapidly increasing activated neutrophils secrete heparin binding protein(HBP)andmyeloperoxidase(MPO)after severe burn injury.Increased HBP triggers vascular leakage with synergy of MPO,results in systemic edema and burn shock.Furthermore,we found that the MPO catalytic product HOCl but not MPO triggers CD44 extracellular domain shedding from vascular endothelial cells to damage the glycocalyx.Damage to the glycocalyx results in firm adhesion of neutrophils and increases vascular leakage.However,MPO inhibitors partially protect the glycocalyx of vascular endothelial cells.The combination of HBP and MPO inhibitors markedly reduces vascular leakage and systemic edema in the early stage of severe burns.Conclusions:Taken together,these data reveal that neutrophil-derived HBP and MPO play an important synergies role in triggering vascular leakage at the early stage of severe burns.Targeted intervention in these two biomolecules may introduce new strategies for helping to reduce large amount of fluid loss and subsequent burn shock.
基金The authors gratefully acknowledge computational time on the Lichtenberg High-Performance Supercomputer.Teng Long thanks the financial support from the China Scholarship Council(CSC).Part of this work was supported by the European Research Council(ERC)under the European Union’s Horizon 2020 research and innovation programme(Grant No.743116-project Cool Innov)This work was also supported by the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)–Project-ID 405553726–TRR 270We also acknowledge support by the Deutsche Forschungsgemeinschaft(DFG–German Research Foundation)and the Open Access Publishing Fund of Technical University of Darmstadt.
文摘Autonomous materials discovery with desired properties is one of the ultimate goals for materials science,and the current studies have been focusing mostly on high-throughput screening based on density functional theory calculations and forward modeling of physical properties using machine learning.Applying the deep learning techniques,we have developed a generative model,which can predict distinct stable crystal structures by optimizing the formation energy in the latent space.It is demonstrated that the optimization of physical properties can be integrated into the generative model as on-top screening or backward propagator,both with their own advantages.Applying the generative models on the binary Bi-Se system reveals that distinct crystal structures can be obtained covering the whole composition range,and the phases on the convex hull can be reproduced after the generated structures are fully relaxed to the equilibrium.The method can be extended to multicomponent systems for multi-objective optimization,which paves the way to achieve the inverse design of materials with optimal properties.
基金supported by the National Natural Science Foundation of China (Grant Nos. 91859201, 92159305, 81971625, and 82030047)
文摘Despite being a common therapy for hepatocellular carcinoma(HCC),insufficient thermal ablation can leave behind tumor residues that can cause recurrence.This is believed to augment M2 inflammatory macrophages that usually play a pro-tumorigenic role.To address this problem,we designed D-mannose-chelated iron oxide nanoparticles(man-IONPs) to polarize M2-like macrophages into the antitumor Ml phenotype.In vitro and in vivo experiments demonstrated that man-IONPs specifically targeted M2-like macrophages and accumulated in peri-ablation zones after macrophage infiltration was augmented under insufficient microwave ablation(MWA).The nanoparticles simultaneously induced polarization of pro-tumorigenic M2 macrophages into antitumor M1 phenotypes,enabling the transformation of the immunosuppressive microenvironment into an immunoactivating one.Post-MWA macrophage polarization exerted robust inhibitory effects on HCC progression in a well-established orthotopic liver cancer mouse model.Thus,combining thermal ablation with man-IONPs can salvage residual tumors after insufficient MWA.These results have strong potential for clinical translation.
基金supported by funds from the National Natural Science Foundation of China(No.31772773,31972678,U1704231)the Innovative Talents of Science and Technology in Universities of Henan Province(20HASTIT040)。
文摘Dear Editor,Porcine deltacoronavirus(PDCoV)is a novel discovered swine enteric coronavirus which can cause diarrhea and dehydration in pigs,particularly in neonatal piglets(Jung et al.,2016).At present,there are no commercial vaccines available for PDCo V.To control PDCoV transmission and perform antiviral therapy efficiently,a rapid and accurate diagnostic method to detect PDCo V is needed.
基金supported by the National Natural Science Foundation of China(NSFC)(No.61627821)the National Key Research and Development Program of China(No.2017YFB0405203)
文摘A novel 1 kHz single-frequency, Q-switched Er-doped yttrium aluminum garnet(Er:YAG) laser pumped by a 1470 nm laser diode is demonstrated. The 500 ns, 5.52 mJ single-frequency, diffraction-limited pulses are obtained by using a ‘ramp-fire' injection-seeding technique and an optical feedback architecture. The full width at half-maximum of the pulse spectrum is measured to be 1.47 MHz by using the heterodyne technique. The beam quality M2 factors are measured to be 1.18 and 1.24 in the x and y directions, respectively.
基金supported by the Huxiang Young Talent Program of Hunan Province (No.2018RS3005)the InnovationDriven Project of Central South University (No.2020CX048)+6 种基金the Changsha Science and Technology Plan Project (Nos.kq2005001,kq2004086)the National Natural Science Foundation of China (No.81301258)the Natural Science Foundation of Hunan Province (Nos.2019JJ60071,2020JJ4680)the Shenghua Yuying Project of Central South University,the Hunan Provincial Graduate Research and Innovation Project (No.CX20190242)National College Students’ Innovation and Entrepreneurship Training Program (Nos.S2020105330414,S202010533015X,8304180412)the Postgraduate Innovation Project of Central South University (Nos.2020zzts819,2020zzts408,2020zzts409,2021zzts0977,2021zzts0979)the Open-End Fund for the Valuable and Precision Instruments of Central South University。
文摘The drug resistance of chemotherapy is a major challenge to overcome for antineoplastic agents and the reverse of drug resistant is essential for cancer therapy. Herein, we developed a drug delivery system which can simultaneously detect/reverse the drug resistance and perform synergetic treatment of cancer. In this work, we integrated cyanine5(Cy5) modified mi RNA(let-7 i)(Cy5-mi RNA) and platinum onto nano-graphene oxide(NGO)(30-50 nm) platform to achieve simultaneously detection/reversion of drug resistance and synergetic treatment of cisplatin resistant SKOV3 cells(SKOV3 DDP cells). The Cy5-mi RNA adsorbed on NGO could selectively bind the drug resistance related m RNA follow by suppress the expression of drug resistance m RNA, and the binding simultaneously induced the release of Cy5-mi RNA from the NGO, thus the fluorescence signal of Cy5 recovered and could be used for drug resistance monitoring.Moreover, the mi RNA suppressed the Cyclin D1 protein expressions thus reversed the drug resistance. The loaded platinum(Ⅳ)(Pt(Ⅳ)) was converted to the therapeutic platinum(Ⅱ)(Pt(Ⅱ)) in both tumor acidic and reductive environment responsive behavior. NGO furtherly performed photothermal therapy under near infrared(NIR) laser irradiation and enhanced the therapeutic effect. All in all, this nanoplatform realized detection/reversion of the drug resistance as well as synergetic chemical-photothermal treatment of ovarian cancer cells, which holds great promise in the treatment of drug resistant cancer cells.
基金supported by National Key Research and Development Program of China(Grant number 2022YFF0711604)the General Project of the NSF of China(Grant number 42071363).
文摘Geographic simulation models can be used to explore and better understand the geographical environment. Recent advances in geographic and socio-environmental research have led to a dramatic increase in the number of models used for this purpose. Some model repositories provide opportunities for users to explore and apply models,but few provide a general evaluation method for assessing the applicability and recognition of models. In this study,an academic impact evaluation method for models is proposed. Five indices are designed based on their pertinence. The analytical hierarchy process is used to calculate the index weights,and the academic impacts of models are quantified with the weighted sum method. The time range is controlled to evaluate the life-term and annual academic impacts of the models. Some models that met the evaluation criteria from different domains are then evaluated. The results show that the academic impact of a model can be quantified with the proposed method,and the major research areas that models impact are identified.
