OBJECTIVE There is a close correlation between interleukin-6 (IL-6) and malignant proliferation of gliomas. We investigated the effect of bradykinin on the expression of IL-6 in C6 glioma cells. METHODS Semi-quantitiv...OBJECTIVE There is a close correlation between interleukin-6 (IL-6) and malignant proliferation of gliomas. We investigated the effect of bradykinin on the expression of IL-6 in C6 glioma cells. METHODS Semi-quantitive RT-PCR and radioimmunoassay were used to examine the effect of bradykinin on the expression of IL-6 in C6 glioma cells and on the level of IL-6 in the culture medium. RESULTS Using semi-quantitive RT-PCR , the expression of IL-6 mRNA was examined in C6 glioma cells at 0, 5, 10,15, 30, 60 min following addition of 1 μmol/L bradykinin. There was no statistical difference in expression of IL-6 mRNA between the treatment and control groups (P>0.05) and IL-6 was not detected in the cell culture medium. CONCLUSION Within an hour, IL-6 expression in C6 glioma cells is not induced by bradykinin, suggesting that its clinical application may be useful as a potential therapeutic agent for tumors of the central nervous system.展开更多
基金supported by grants from the National Natural Science Foundation of China (No.30670723, No.30600060, No.30400145 and No.30570650)Natu-ral Science Foundation of Liaoning Province (No.20052102).
文摘OBJECTIVE There is a close correlation between interleukin-6 (IL-6) and malignant proliferation of gliomas. We investigated the effect of bradykinin on the expression of IL-6 in C6 glioma cells. METHODS Semi-quantitive RT-PCR and radioimmunoassay were used to examine the effect of bradykinin on the expression of IL-6 in C6 glioma cells and on the level of IL-6 in the culture medium. RESULTS Using semi-quantitive RT-PCR , the expression of IL-6 mRNA was examined in C6 glioma cells at 0, 5, 10,15, 30, 60 min following addition of 1 μmol/L bradykinin. There was no statistical difference in expression of IL-6 mRNA between the treatment and control groups (P>0.05) and IL-6 was not detected in the cell culture medium. CONCLUSION Within an hour, IL-6 expression in C6 glioma cells is not induced by bradykinin, suggesting that its clinical application may be useful as a potential therapeutic agent for tumors of the central nervous system.
