Background:The“flipped classroom”is a learner-centered approach that centers on delivering videos,podcasts or slide-based material to learners prior to a lecture or class session.The class session is then dedicated ...Background:The“flipped classroom”is a learner-centered approach that centers on delivering videos,podcasts or slide-based material to learners prior to a lecture or class session.The class session is then dedicated to discussion,analysis,and problem-solving activities.The aim of this study was to investigate whether the flipped classroom could be adapted to medical(ophthalmology)students learning about ocular trauma and to assess the impact of the flipped classroom on those students’performance and attitudes.Methods:Questionnaires(using a 4-point scale)were distributed to 93 fifth-year medical students at Sun Yat-sen University,and the data showed that the majority of students preferred the flipped classroom approach to the traditional lecture method.Results:The results of pre-and post-test scores were 14.35±3.404 and 20.37±4.356,which showed a significant improvement in students’performance after the flipped classroom was introduced(P<0.05).Conclusions:Student response to the flipped classroom strategy was largely positive,indicating that the strategy received a high level of approval in an ophthalmology clerkship course taken by medical students in China.展开更多
Monitoring physiological signals of the human body can provide extremely important information for sports healthcare,preventing injuries and providing efficient guidance for individual sports.However,the signals relat...Monitoring physiological signals of the human body can provide extremely important information for sports healthcare,preventing injuries and providing efficient guidance for individual sports.However,the signals related to human healthcare involve both subtle and vigorous signals,making it difficult for a sensor to satisfy the full-scale monitoring at the same time.Here,a novel conductive elastomer featuring homogeneously micropyramid-structured PDMS/CNT composite is used to fabricate highperformance piezoresistive sensors by a drop-casting method.Benefiting from the significant increase in the contact area of microstructure during deformation,the flexible sensor presents a broad detection range(up to 185.5 kPa),fast response/recovery time(44/13 ms),ultrahigh sensitivity(242.4 kPa–1)and excellent durability over 8,000 cycles.As a proof of concept,the as-fabricated pressure sensor can be used for body-area sports healthcare,and enable the detection of full-scale pressure distribution.Considering the fabulous sensing performance,the sensor may potentially become promising in personal sports healthcare and telemedicine monitoring.展开更多
Finely tuning mechanosensitive membrane proteins holds great potential in precisely controlling inflammatory responses.In addition to macroscopic force,mechanosensitive membrane proteins are reported to be sensitive t...Finely tuning mechanosensitive membrane proteins holds great potential in precisely controlling inflammatory responses.In addition to macroscopic force,mechanosensitive membrane proteins are reported to be sensitive to micro-nano forces.Integrinβ_(2),for example,might undergo a piconewton scale stretching force in the activation state.High-aspect-ratio nanotopographic structures were found to generate nN-scale biomechanical force.Together with the advantages of uniform and precisely tunable structural parameters,it is fascinating to develop low-aspect-ratio nanotopographic structures to generate micro-nano forces for finely modulating their conformations and the subsequent mechanoimmiune responses.In this study,low-aspect-ratio nanotopographic structures were developed to finely manipulate the conformation of integrinβ_(2).The direct interaction of forces and the model molecule integrinαXβ_(2)was first performed.It was demonstrated that pressing force could successfully induce conformational compression and deactivation of integrinαXβ_(2),and approximately 270 to 720 pN may be required to inhibit its conformational extension and activation.Three low-aspect-ratio nanotopographic surfaces(nanohemispheres,nanorods,and nanoholes)with various structural parameters were specially designed to generate the micro-nano forces.It was found that the nanorods and nanohemispheres surfaces induce greater contact pressure at the contact interface between macrophages and nanotopographic structures,particularly after cell adhesion.These higher contact pressures successfully inhibited the conformational extension and activation of integrinβ_(2),suppressing focal adhesion activity and the downstream PI3K-Akt signaling pathway,reducing NF-κB signaling and macrophage inflammatory responses.Our findings suggest that nanotopographic structures can be used to finely tune mechanosensitive membrane protein conformation changes,providing an effective strategy for precisely modulating inflammatory responses.展开更多
We study the following coupled system of quasilinear equations:Under some assumptions on the nonlinear terms f and g, we establish some results about the existence and regularitl of vector solutions for the p-Laplacia...We study the following coupled system of quasilinear equations:Under some assumptions on the nonlinear terms f and g, we establish some results about the existence and regularitl of vector solutions for the p-Laplacian systems by using variational methods. In particular, we get two pairs of nontrivial solutions. We also study the different asymptotic behavior of solutions as the coupling parameter λ tends to zero.展开更多
Micro/nano topographic structures have shown great utility in many biomedical areas including cell therapies,tissue engineering,and implantable devices.Computer-assisted informatics methods hold great promise for the ...Micro/nano topographic structures have shown great utility in many biomedical areas including cell therapies,tissue engineering,and implantable devices.Computer-assisted informatics methods hold great promise for the design of topographic structures with targeted properties for a specific medical application.To benefit from these methods,researchers and engineers require a highly reusable“one structural parameter-one set of cell responses”database.However,existing confounding factors in topographic cell culture devices seriously impede the acquisition of this kind of data.Through carefully dissecting the confounding factors and their possible reasons for emergence,we developed corresponding guideline requirements for topographic cell culture device development to remove or control the influence of such factors.Based on these requirements,we then suggested potential strategies to meet them.In this work,we also experimentally demonstrated a topographic cell culture device with controlled confounding factors based on these guideline requirements and corresponding strategies.A“guideline for the development of topographic cell culture devices”was summarized to instruct researchers to develop topographic cell culture devices with the confounding factors removed or well controlled.This guideline aims to promote the establishment of a highly reusable“one structural parameter-one set of cell responses”database that could facilitate the application of informatics methods,such as artificial intelligence,in the rational design of future biotopographic structures with high efficacy.展开更多
基金All the procedures in this study were approved by the Institutional Review Board of Zhongshan Ophthalmic Center of Sun Yat-sen University(Ethic ID:2016MEKY062).Informed consent was obtained from each student.
文摘Background:The“flipped classroom”is a learner-centered approach that centers on delivering videos,podcasts or slide-based material to learners prior to a lecture or class session.The class session is then dedicated to discussion,analysis,and problem-solving activities.The aim of this study was to investigate whether the flipped classroom could be adapted to medical(ophthalmology)students learning about ocular trauma and to assess the impact of the flipped classroom on those students’performance and attitudes.Methods:Questionnaires(using a 4-point scale)were distributed to 93 fifth-year medical students at Sun Yat-sen University,and the data showed that the majority of students preferred the flipped classroom approach to the traditional lecture method.Results:The results of pre-and post-test scores were 14.35±3.404 and 20.37±4.356,which showed a significant improvement in students’performance after the flipped classroom was introduced(P<0.05).Conclusions:Student response to the flipped classroom strategy was largely positive,indicating that the strategy received a high level of approval in an ophthalmology clerkship course taken by medical students in China.
基金This work was financially supported by the National Natural Science Foundation of China(No.61801403)the Sichuan province Foundation for Distinguished Young Team(No.20CXTD0106)the Basic Research Cultivation Project(No.2682021ZTPY004).
文摘Monitoring physiological signals of the human body can provide extremely important information for sports healthcare,preventing injuries and providing efficient guidance for individual sports.However,the signals related to human healthcare involve both subtle and vigorous signals,making it difficult for a sensor to satisfy the full-scale monitoring at the same time.Here,a novel conductive elastomer featuring homogeneously micropyramid-structured PDMS/CNT composite is used to fabricate highperformance piezoresistive sensors by a drop-casting method.Benefiting from the significant increase in the contact area of microstructure during deformation,the flexible sensor presents a broad detection range(up to 185.5 kPa),fast response/recovery time(44/13 ms),ultrahigh sensitivity(242.4 kPa–1)and excellent durability over 8,000 cycles.As a proof of concept,the as-fabricated pressure sensor can be used for body-area sports healthcare,and enable the detection of full-scale pressure distribution.Considering the fabulous sensing performance,the sensor may potentially become promising in personal sports healthcare and telemedicine monitoring.
基金This work was financially supported by the National Natural Science Foundation of China(No.82061167)the National Key Research and Development Program of China(No.2022YFA1104400)+3 种基金the International Team for Implantology(ITI)Research Grant(No.1536_2020)Fundamental Research Funds of Sun Yat-sen University(No.22ykqb06)Science and Technology Program of Guangzhou(No.SL2022B03J00507)Guangdong Financial Fund for High-Caliber Hospital Construction,and National Undergraduate Training Program for Innovation and Entrepreneurship(No.202210772).
文摘Finely tuning mechanosensitive membrane proteins holds great potential in precisely controlling inflammatory responses.In addition to macroscopic force,mechanosensitive membrane proteins are reported to be sensitive to micro-nano forces.Integrinβ_(2),for example,might undergo a piconewton scale stretching force in the activation state.High-aspect-ratio nanotopographic structures were found to generate nN-scale biomechanical force.Together with the advantages of uniform and precisely tunable structural parameters,it is fascinating to develop low-aspect-ratio nanotopographic structures to generate micro-nano forces for finely modulating their conformations and the subsequent mechanoimmiune responses.In this study,low-aspect-ratio nanotopographic structures were developed to finely manipulate the conformation of integrinβ_(2).The direct interaction of forces and the model molecule integrinαXβ_(2)was first performed.It was demonstrated that pressing force could successfully induce conformational compression and deactivation of integrinαXβ_(2),and approximately 270 to 720 pN may be required to inhibit its conformational extension and activation.Three low-aspect-ratio nanotopographic surfaces(nanohemispheres,nanorods,and nanoholes)with various structural parameters were specially designed to generate the micro-nano forces.It was found that the nanorods and nanohemispheres surfaces induce greater contact pressure at the contact interface between macrophages and nanotopographic structures,particularly after cell adhesion.These higher contact pressures successfully inhibited the conformational extension and activation of integrinβ_(2),suppressing focal adhesion activity and the downstream PI3K-Akt signaling pathway,reducing NF-κB signaling and macrophage inflammatory responses.Our findings suggest that nanotopographic structures can be used to finely tune mechanosensitive membrane protein conformation changes,providing an effective strategy for precisely modulating inflammatory responses.
文摘We study the following coupled system of quasilinear equations:Under some assumptions on the nonlinear terms f and g, we establish some results about the existence and regularitl of vector solutions for the p-Laplacian systems by using variational methods. In particular, we get two pairs of nontrivial solutions. We also study the different asymptotic behavior of solutions as the coupling parameter λ tends to zero.
基金financially supported by National Natural Science Foundation of China(82071167)Natural Science Foundation of Guangdong Province(2018B030306030)+2 种基金International Team for Implantology(ITI)Research Grant(1536_2020)Guangdong Financial Fund for High-Caliber Hospital ConstructionSpecial Funds for the Cultivation of Guangdong College Students’Scientific and Technological Innovation(“Climbing Program”Special Funds,pdjh2020b0011).
文摘Micro/nano topographic structures have shown great utility in many biomedical areas including cell therapies,tissue engineering,and implantable devices.Computer-assisted informatics methods hold great promise for the design of topographic structures with targeted properties for a specific medical application.To benefit from these methods,researchers and engineers require a highly reusable“one structural parameter-one set of cell responses”database.However,existing confounding factors in topographic cell culture devices seriously impede the acquisition of this kind of data.Through carefully dissecting the confounding factors and their possible reasons for emergence,we developed corresponding guideline requirements for topographic cell culture device development to remove or control the influence of such factors.Based on these requirements,we then suggested potential strategies to meet them.In this work,we also experimentally demonstrated a topographic cell culture device with controlled confounding factors based on these guideline requirements and corresponding strategies.A“guideline for the development of topographic cell culture devices”was summarized to instruct researchers to develop topographic cell culture devices with the confounding factors removed or well controlled.This guideline aims to promote the establishment of a highly reusable“one structural parameter-one set of cell responses”database that could facilitate the application of informatics methods,such as artificial intelligence,in the rational design of future biotopographic structures with high efficacy.
