Objective: To study the methods for diagnosis andtreatment of insulinoma.Methods: Clinical data from 105 patients with insuli-noma who had been admitted to our hospital from Ju-ly 1966 to December 1999 were retrospect...Objective: To study the methods for diagnosis andtreatment of insulinoma.Methods: Clinical data from 105 patients with insuli-noma who had been admitted to our hospital from Ju-ly 1966 to December 1999 were retrospectively re-viewed.Results: Fasting blood glucose values were less than2.75 mmol/L in all the patients. Fasting serum insulinvalues in 60 patients were higher than 25 mU/L, av-erage 65 mU/L. Before operation, carcinoma was de-tected in 2 of 45 patients by ultrasound scan, and in10 of 35 by CT. Enucleation of insulinoma was per-formed in 60 patients. Operations included insulinomaresection (35 patients), distal resection of the pancreas(8), and biopsy (2).Conclusion: Whipple’s triad and the index of insulinrelease】0.3 are the major variables for diagno-sis Intraoperative exploration and ultrasound scan are themethods for the localization of insulinoma Enucleation ofbenign insulinoma is preferred, but proximal or distal re-sections of the pancreas are required only for large, deep ormultiple展开更多
The effect of dissolved oxygen(DO) on the stress corrosion cracking(SCC) of 310 S in supercritical water was investigated using slow-strain-rate tensile tests.The tensile properties, fracture morphology, and distribut...The effect of dissolved oxygen(DO) on the stress corrosion cracking(SCC) of 310 S in supercritical water was investigated using slow-strain-rate tensile tests.The tensile properties, fracture morphology, and distribution of the chemical composition of the oxide were analyzed to evaluate the SCC susceptibility of 310 S. The results showed that the rupture elongation decreased significantly as the degree of DO increased. A brittle fracture mode was observed on the fracture surface, and only intergranular cracking was observed on the surface of the gauge section, regardless of the DO. Cracks were widely distributed on the gauge surface near the fracture surface.Oxides were observed in the cracks with two-layered structures, i.e., a Cr-rich inner oxide layer and an Fe-rich outer oxide layer.展开更多
AIM: To investigate the effects of Axl deglycosylation on tumor lymphatic metastases in mouse hepatocellular carcinoma cell lines. METHODS: Western blotting was used to analyze the expression profile of Axl glycoprote...AIM: To investigate the effects of Axl deglycosylation on tumor lymphatic metastases in mouse hepatocellular carcinoma cell lines. METHODS: Western blotting was used to analyze the expression profile of Axl glycoprotein in mouse hepa-tocellular carcinoma cell line Hca-F treated with tunicamycin and PNGase F 3-(4,5)-dimethylthiazol(-zyl)-3,5- diphenyltetrazolium bromide (MTT) assay, extracellular matrix (ECM) invasion assay (in vitro ) and tumor metastasis assay (in vivo ) were utilized to evaluate the effect of Axl deglycosylation on the Hca-F cell proliferation, invasion and lymphatic metastasis. RESULTS: Tunicamycin and PNGase F treatment markedly inhibited Axl glycoprotein synthesis and expression, proliferation, invasion, and lymphatic metastasis both in vitro and in vivo . In the MTT assay, proliferation was apparent in untreated Hca-F cells compared with treated Hca-F cells. In the ECM invasion assay (in vitro ), treated cells passed through the ECMatrix gel in significantly smaller numbers than untreated cells (tunicamycin 5 μg/mL: 68 ± 8 vs 80 ± 9, P=0.0222; 10 μg/mL: 50 ± 6vs 80 ± 9,P=0.0003; 20 μg/mL: 41 ± 4 vs 80 ± 9, P=0.0001); (PNGase F 8 h: 66 ± 7 vs 82 ± 8, P=0.0098; 16 h: 49 ± 4 vs 82 ± 8, P=0.0001; 24 h: 34 ± 3 vs 82 ± 8, P=0.0001). In the tumor metastasis assay (in vivo ), average lymph node weights of the untreated Hca-F group compared with treated Hca-F groups (tunicamycin 5 μg/mL: 0.84 ± 0.21 g vs 0.72 ± 0.19 g, P=0.3237; 10 μg/mL: 0.84 ± 0.21 g vs 0.54 ± 0.11 g, P=0.0113; 20 μg/mL: 0.84 ± 0.21 g vs 0.42 ± 0.06 g, P=0.0008); (PNGase F 8 h: 0.79 ± 0.15 g vs 0.63 ± 0.13 g, P=0.0766; 16 h: 0.79 ± 0.15 g vs 0.49 ± 0.10 g, P=0.0022; 24 h: 0.79 ± 0.15 g vs 0.39 ± 0.05 g, P=0.0001). Also, average lymph node volumes of the untreated Hca-F group compared with treated Hca-F groups (tunicamycin 5 μg/mL: 815 ± 61 mm 3 vs 680 ± 59 mm 3 , P=0.0613; 10 μg/mL: 815 ± 61 mm 3 vs 580 ± 29 mm 3 , P=0.0001; 20 μg/mL: 815 ± 61 mm 3 vs 395 ± 12 mm 3 , P=0.0001); (PNGase F 8 h: 670 ± 56 mm 3 vs 581 ± 48 mm 3 , P=0.0532; 16 h: 670 ± 56 mm 3 vs 412 ± 22 mm 3 , P=0.0001; 24 h: 670 ± 56 mm 3 vs 323 ± 11 mm 3 , P=0.0001). CONCLUSION: Alteration of Axl glycosylation can at-tenuate neoplastic lymphatic metastasis. Axl N-glycans may be a universal target for chemotherapy.展开更多
OBJECTIVE: To investigate the protective mechanism of different ischemic preconditioning （IPC） toischemia/reperfusion （I/R） injury of rat liver graft.METHODS: 192 Wistar rats were randomly divided into 4 groups （...OBJECTIVE: To investigate the protective mechanism of different ischemic preconditioning （IPC） toischemia/reperfusion （I/R） injury of rat liver graft.METHODS: 192 Wistar rats were randomly divided into 4 groups （48 rats in each group）: control group（group C）, experimental group 1 （group E<sub>1</sub>）, experimental group 2 （group E<sub>2</sub>）, and experimental group 3（group E<sub>3</sub>）. IPC was not carried out in group C. In the experimental groups, IPC was carried out byblocking blood flow of the portal vein and hepatic artery and then reperfusion by removal of the clampbefore donor liver was resected. Group E<sub>1</sub>: 5-minute ischemia and 10-minute reperfusion; Group E<sub>2</sub>:5-minute ischemia and 5-minute reperfusion and one more the same procedure; Group E<sub>3</sub>: 10-minuteischemia and 15-minute reperfusion. Four hours after IPC, liver transplantations were performed.Recipient blood and graft samples were obtained to determine the levels of ALT, AST, TNF-α andapoptosis index at 0.5, 2, 6, 24 hours after portal vein reperfusion.RESULTS: At 0.5, 2 hours after portal vein reperfusion, the levels of TNF-α in the experimental groupsE<sub>1</sub>, E<sub>2</sub>, and E<sub>3</sub> were significantly lower than in the control group （P【0.05）, and the levels in groupE<sub>2</sub> were significantly lower than in groups E<sub>1</sub> and E<sub>3</sub> （P【0.05）. At 24 hours, the levels of TNF-α ingroup E<sub>2</sub> were significantly lower than in groups C, E<sub>1</sub> anti E<sub>3</sub> （P【0.05）. At 2 and 6 hours, apoptosisindex in the experimental groups E<sub>1</sub>, E<sub>2</sub>, and E<sub>3</sub> was significantly less than in the control group（P【0.05）. Apoptosis index in group E<sub>2</sub> was significantly less than groups E<sub>1</sub> and E<sub>3</sub>（P【0.05）. At 24hours apoptosis index in the experimental groups E<sub>1</sub>, E<sub>2</sub> , and E<sub>3</sub> was significantly less than in the controlgroup （P【0.05）.CONCLUSIONS: Ischemic preconditioning could attenuate liver graft injury by decreasing apoptosis ofhepatocytes and production of TNF-α. The method of IPC with 5-minute ischemia, 5-minute reperfusionand one more the same procedure is a better way to protect liver graft from ischemia-reperfusion injury.展开更多
Poly(vinyl acetate)(PVAc) was grafted onto wheat straw by γ-irradiation to improve the compatibility between wheat straw and high-density polyethelene(PE).The grafting was proved by Fourier transform infrared(FTIR) s...Poly(vinyl acetate)(PVAc) was grafted onto wheat straw by γ-irradiation to improve the compatibility between wheat straw and high-density polyethelene(PE).The grafting was proved by Fourier transform infrared(FTIR) spectroscopy. The compact structure of wheat straw was loosened because the chemical bonds and crystalline structure were destructed by the γ-rays. The modified wheat straw needed less energy for thermal transition, as revealed by differential scanning calorimetry(DSC).Thermal analysis revealed that grafted PVAc acted as a protective barrier for the wheat straw and leads to an increase in maximum pyrolysis temperature. The crystallite size of grafted wheat straw decreased to 5.33 nm from 5.63 nm before irradiation. There were holes in melted form appeared on the surface of the grafted wheat straws.Both the grafted PVAc and irradiation are beneficial to lower the torque of wheat straw/PE melts and improve its mechanical properties by 36%. Possible mechanism of irradiation grafting was proposed.展开更多
Objective:To investigate the protective effects and mechanism of antioxidant TBHQ on renal damage caused by doxorubicin chemotherapy in mice with hepatic cancer.Methods:Cell H22 of mice with hepatic cancer which was s...Objective:To investigate the protective effects and mechanism of antioxidant TBHQ on renal damage caused by doxorubicin chemotherapy in mice with hepatic cancer.Methods:Cell H22 of mice with hepatic cancer which was subcultured for three times was subcutaneously transplanted to the groin of right lower limb of 45 SPF Kunming mice to establish the transplanted tumor model.The doxorubicin chemotherapy group and antioxidant intervention group received intraperitoneal injection of ADM(1 mg/kg·0.2 mL/2d).The model control group received normal saline(NS) of the same volume at the same time.1%TBHQ was added into the diet of mice of the antioxidant intervention group.Seven weeks later,morning urines and peripheral blood were randomly collected to detect UAIb,UCr,BUN,Scr and UAlb/Cr levels.All mice were beheaded.The renal tissues were made into homogenate,and SOD,T-AOC and MDA content in tissues were detected followed by cell lysis.All data were processed using SPSS 19.0.Results:The UAlb/Cr,BUN.Scr and MDA of doxorubicin chemotherapy group were significantly higher those of model control group and the activities of SOD,T-AOC in doxorubicin chemotherapy group were lower than those of model control group(P<0.01).The UAlb/Cr,BUN,Scr and MDA of antioxidant intervention group were lower than those of doxorubicin chemotherapy group and the activities of SOD,T-AOC of antioxidant intervention group were higher than those of doxorubicin chemotherapy group doxorubicin chemotherapy group(P<0.05).The BUN of model control group was higher than that of blank group,and T-AOC was lower than that of blank group,and difference was statistically significant(P<0.05).Conclusions:Doxorubicin chemotherapy could lead to abnormal antioxidant capacity and renal function of tumor-bearing mice with hepatic cancer.TBHQ antioxidant intervention could effectively improve the antioxidant capacity of renal tissue and reduce the renal damage caused by doxorubicin to some extent.展开更多
文摘Objective: To study the methods for diagnosis andtreatment of insulinoma.Methods: Clinical data from 105 patients with insuli-noma who had been admitted to our hospital from Ju-ly 1966 to December 1999 were retrospectively re-viewed.Results: Fasting blood glucose values were less than2.75 mmol/L in all the patients. Fasting serum insulinvalues in 60 patients were higher than 25 mU/L, av-erage 65 mU/L. Before operation, carcinoma was de-tected in 2 of 45 patients by ultrasound scan, and in10 of 35 by CT. Enucleation of insulinoma was per-formed in 60 patients. Operations included insulinomaresection (35 patients), distal resection of the pancreas(8), and biopsy (2).Conclusion: Whipple’s triad and the index of insulinrelease】0.3 are the major variables for diagno-sis Intraoperative exploration and ultrasound scan are themethods for the localization of insulinoma Enucleation ofbenign insulinoma is preferred, but proximal or distal re-sections of the pancreas are required only for large, deep ormultiple
基金supported by the National Natural Science Foundation of China(Nos.51271171 and 11775150)
文摘The effect of dissolved oxygen(DO) on the stress corrosion cracking(SCC) of 310 S in supercritical water was investigated using slow-strain-rate tensile tests.The tensile properties, fracture morphology, and distribution of the chemical composition of the oxide were analyzed to evaluate the SCC susceptibility of 310 S. The results showed that the rupture elongation decreased significantly as the degree of DO increased. A brittle fracture mode was observed on the fracture surface, and only intergranular cracking was observed on the surface of the gauge section, regardless of the DO. Cracks were widely distributed on the gauge surface near the fracture surface.Oxides were observed in the cracks with two-layered structures, i.e., a Cr-rich inner oxide layer and an Fe-rich outer oxide layer.
基金Supported by Creating Team Item of Liaoning Province, No.2008T033the Technological Natural Fund Item of Liaoning Province, China, No. 20092164
文摘AIM: To investigate the effects of Axl deglycosylation on tumor lymphatic metastases in mouse hepatocellular carcinoma cell lines. METHODS: Western blotting was used to analyze the expression profile of Axl glycoprotein in mouse hepa-tocellular carcinoma cell line Hca-F treated with tunicamycin and PNGase F 3-(4,5)-dimethylthiazol(-zyl)-3,5- diphenyltetrazolium bromide (MTT) assay, extracellular matrix (ECM) invasion assay (in vitro ) and tumor metastasis assay (in vivo ) were utilized to evaluate the effect of Axl deglycosylation on the Hca-F cell proliferation, invasion and lymphatic metastasis. RESULTS: Tunicamycin and PNGase F treatment markedly inhibited Axl glycoprotein synthesis and expression, proliferation, invasion, and lymphatic metastasis both in vitro and in vivo . In the MTT assay, proliferation was apparent in untreated Hca-F cells compared with treated Hca-F cells. In the ECM invasion assay (in vitro ), treated cells passed through the ECMatrix gel in significantly smaller numbers than untreated cells (tunicamycin 5 μg/mL: 68 ± 8 vs 80 ± 9, P=0.0222; 10 μg/mL: 50 ± 6vs 80 ± 9,P=0.0003; 20 μg/mL: 41 ± 4 vs 80 ± 9, P=0.0001); (PNGase F 8 h: 66 ± 7 vs 82 ± 8, P=0.0098; 16 h: 49 ± 4 vs 82 ± 8, P=0.0001; 24 h: 34 ± 3 vs 82 ± 8, P=0.0001). In the tumor metastasis assay (in vivo ), average lymph node weights of the untreated Hca-F group compared with treated Hca-F groups (tunicamycin 5 μg/mL: 0.84 ± 0.21 g vs 0.72 ± 0.19 g, P=0.3237; 10 μg/mL: 0.84 ± 0.21 g vs 0.54 ± 0.11 g, P=0.0113; 20 μg/mL: 0.84 ± 0.21 g vs 0.42 ± 0.06 g, P=0.0008); (PNGase F 8 h: 0.79 ± 0.15 g vs 0.63 ± 0.13 g, P=0.0766; 16 h: 0.79 ± 0.15 g vs 0.49 ± 0.10 g, P=0.0022; 24 h: 0.79 ± 0.15 g vs 0.39 ± 0.05 g, P=0.0001). Also, average lymph node volumes of the untreated Hca-F group compared with treated Hca-F groups (tunicamycin 5 μg/mL: 815 ± 61 mm 3 vs 680 ± 59 mm 3 , P=0.0613; 10 μg/mL: 815 ± 61 mm 3 vs 580 ± 29 mm 3 , P=0.0001; 20 μg/mL: 815 ± 61 mm 3 vs 395 ± 12 mm 3 , P=0.0001); (PNGase F 8 h: 670 ± 56 mm 3 vs 581 ± 48 mm 3 , P=0.0532; 16 h: 670 ± 56 mm 3 vs 412 ± 22 mm 3 , P=0.0001; 24 h: 670 ± 56 mm 3 vs 323 ± 11 mm 3 , P=0.0001). CONCLUSION: Alteration of Axl glycosylation can at-tenuate neoplastic lymphatic metastasis. Axl N-glycans may be a universal target for chemotherapy.
文摘OBJECTIVE: To investigate the protective mechanism of different ischemic preconditioning （IPC） toischemia/reperfusion （I/R） injury of rat liver graft.METHODS: 192 Wistar rats were randomly divided into 4 groups （48 rats in each group）: control group（group C）, experimental group 1 （group E<sub>1</sub>）, experimental group 2 （group E<sub>2</sub>）, and experimental group 3（group E<sub>3</sub>）. IPC was not carried out in group C. In the experimental groups, IPC was carried out byblocking blood flow of the portal vein and hepatic artery and then reperfusion by removal of the clampbefore donor liver was resected. Group E<sub>1</sub>: 5-minute ischemia and 10-minute reperfusion; Group E<sub>2</sub>:5-minute ischemia and 5-minute reperfusion and one more the same procedure; Group E<sub>3</sub>: 10-minuteischemia and 15-minute reperfusion. Four hours after IPC, liver transplantations were performed.Recipient blood and graft samples were obtained to determine the levels of ALT, AST, TNF-α andapoptosis index at 0.5, 2, 6, 24 hours after portal vein reperfusion.RESULTS: At 0.5, 2 hours after portal vein reperfusion, the levels of TNF-α in the experimental groupsE<sub>1</sub>, E<sub>2</sub>, and E<sub>3</sub> were significantly lower than in the control group （P【0.05）, and the levels in groupE<sub>2</sub> were significantly lower than in groups E<sub>1</sub> and E<sub>3</sub> （P【0.05）. At 24 hours, the levels of TNF-α ingroup E<sub>2</sub> were significantly lower than in groups C, E<sub>1</sub> anti E<sub>3</sub> （P【0.05）. At 2 and 6 hours, apoptosisindex in the experimental groups E<sub>1</sub>, E<sub>2</sub>, and E<sub>3</sub> was significantly less than in the control group（P【0.05）. Apoptosis index in group E<sub>2</sub> was significantly less than groups E<sub>1</sub> and E<sub>3</sub>（P【0.05）. At 24hours apoptosis index in the experimental groups E<sub>1</sub>, E<sub>2</sub> , and E<sub>3</sub> was significantly less than in the controlgroup （P【0.05）.CONCLUSIONS: Ischemic preconditioning could attenuate liver graft injury by decreasing apoptosis ofhepatocytes and production of TNF-α. The method of IPC with 5-minute ischemia, 5-minute reperfusionand one more the same procedure is a better way to protect liver graft from ischemia-reperfusion injury.
基金supported by the National Natural Science Foundation of China(No.11605077)the Free Exploration Project for Youth Research of Jiangsu Academy of Agricultural Sciences(No.ZX(15)4012)
文摘Poly(vinyl acetate)(PVAc) was grafted onto wheat straw by γ-irradiation to improve the compatibility between wheat straw and high-density polyethelene(PE).The grafting was proved by Fourier transform infrared(FTIR) spectroscopy. The compact structure of wheat straw was loosened because the chemical bonds and crystalline structure were destructed by the γ-rays. The modified wheat straw needed less energy for thermal transition, as revealed by differential scanning calorimetry(DSC).Thermal analysis revealed that grafted PVAc acted as a protective barrier for the wheat straw and leads to an increase in maximum pyrolysis temperature. The crystallite size of grafted wheat straw decreased to 5.33 nm from 5.63 nm before irradiation. There were holes in melted form appeared on the surface of the grafted wheat straws.Both the grafted PVAc and irradiation are beneficial to lower the torque of wheat straw/PE melts and improve its mechanical properties by 36%. Possible mechanism of irradiation grafting was proposed.
文摘Objective:To investigate the protective effects and mechanism of antioxidant TBHQ on renal damage caused by doxorubicin chemotherapy in mice with hepatic cancer.Methods:Cell H22 of mice with hepatic cancer which was subcultured for three times was subcutaneously transplanted to the groin of right lower limb of 45 SPF Kunming mice to establish the transplanted tumor model.The doxorubicin chemotherapy group and antioxidant intervention group received intraperitoneal injection of ADM(1 mg/kg·0.2 mL/2d).The model control group received normal saline(NS) of the same volume at the same time.1%TBHQ was added into the diet of mice of the antioxidant intervention group.Seven weeks later,morning urines and peripheral blood were randomly collected to detect UAIb,UCr,BUN,Scr and UAlb/Cr levels.All mice were beheaded.The renal tissues were made into homogenate,and SOD,T-AOC and MDA content in tissues were detected followed by cell lysis.All data were processed using SPSS 19.0.Results:The UAlb/Cr,BUN.Scr and MDA of doxorubicin chemotherapy group were significantly higher those of model control group and the activities of SOD,T-AOC in doxorubicin chemotherapy group were lower than those of model control group(P<0.01).The UAlb/Cr,BUN,Scr and MDA of antioxidant intervention group were lower than those of doxorubicin chemotherapy group and the activities of SOD,T-AOC of antioxidant intervention group were higher than those of doxorubicin chemotherapy group doxorubicin chemotherapy group(P<0.05).The BUN of model control group was higher than that of blank group,and T-AOC was lower than that of blank group,and difference was statistically significant(P<0.05).Conclusions:Doxorubicin chemotherapy could lead to abnormal antioxidant capacity and renal function of tumor-bearing mice with hepatic cancer.TBHQ antioxidant intervention could effectively improve the antioxidant capacity of renal tissue and reduce the renal damage caused by doxorubicin to some extent.
