Background:Osteoporosis is a chronic bone disease characterized by bone loss and decreased bone strength.However,current anti-resorptive drugs carry a risk of various complications.The deep learning-based efficacy pre...Background:Osteoporosis is a chronic bone disease characterized by bone loss and decreased bone strength.However,current anti-resorptive drugs carry a risk of various complications.The deep learning-based efficacy prediction system(DLEPS)is a forecasting tool that can effectively compete in drug screening and prediction based on gene expression changes.This study aimed to explore the protective effect and potential mechanisms of cinobufotalin(CB),a traditional Chinese medicine(TCM),on bone loss.Methods:DLEPS was employed for screening anti-osteoporotic agents according to gene profile changes in primary osteoporosis.Micro-CT,histological and morphological analysis were applied for the bone protective detection of CB,and the osteogenic differentiation/function in human bone marrow mesenchymal stem cells(hBMMSCs)were also investigated.The underlying mechanism was verified using qRT-PCR,Western blot(WB),immunofluorescence(IF),etc.Results:A safe concentration(0.25mg/kg in vivo,0.05μM in vitro)of CB could effectively preserve bone mass in estrogen deficiency-induced bone loss and promote osteogenic differentiation/function of hBMMSCs.Both BMPs/SMAD and Wnt/β-catenin signaling pathways participated in CB-induced osteogenic differentiation,further regulating the expression of osteogenesis-associated factors,and ultimately promoting osteogenesis.Conclusion:Our study demonstrated that CB could significantly reverse estrogen deficiency-induced bone loss,further promoting osteogenic differentiation/function of hBMMSCs,with BMPs/SMAD and Wnt/β-catenin signaling pathways involved.展开更多
The presence of a large palatal or maxillary defect after partial or total maxillectomy for tumor, trauma or congenital deformation poses a challenge to prosthodontists, particularly when the use of an implant cannot ...The presence of a large palatal or maxillary defect after partial or total maxillectomy for tumor, trauma or congenital deformation poses a challenge to prosthodontists, particularly when the use of an implant cannot be considered. This case report described the use of an air valve in a hollow silicone obturator to manufacture an inflatable obturator that could be extended further into undercut area to retain itself. The inflatable obturator exhibited adequate retention, stability and border sealing, thereby improving the masticatory, pronunciation and swallowing functions of patients. It may be a suitable alternative treatment option to an implant-retained obturator.展开更多
The internal flow field of an axial-flow pump under low flow rate conditions is extremely turbulent. The unstable flow region is formed due to the tip leakage and the rotating stall, and is distinguished by a saddle p...The internal flow field of an axial-flow pump under low flow rate conditions is extremely turbulent. The unstable flow region is formed due to the tip leakage and the rotating stall, and is distinguished by a saddle patterned zone in its flow-head curve that demonstrates restrictions in its workable flow range. It is therefore important to understand and improve the operational characteristics of an axial-flow pump under low flow rate conditions. In this study, experiments are performed for the performances of an axial-flow pump at the flow rate in a range between 0.8Qd and 1.2Qd, with the internal flow field measured by the particle image velocimetry (PIV), and with the pump performances and the internal flow field simulated by the Ansys CFX. The simulation results agree well with the experimental results. From the predicted heads at the flow rate in the range between 0.1Qd and 0.7Qd by the numerical simulation, the complete flow-head curves are obtained. The streamlines and the velocity contours in the region in front of the impeller leading edge under different flow conditions are analyzed. By adopting the double-inlet structures, the flow-head characteristics are studied by varying the values of δ and σ respectively, where δ denotes the gap between the inner cylinder end and the impeller leading edge, and σ denotes the gap between the inner cylinder and the outer cylinder. The findings indicate that with the double-inlet design, the velocity distribution can be effectively improved in the region in front of the impeller leading edge, as well as the head performance under the low flow rate conditions, and the positive slope value of the head curve is reduced in the unstable flow range. The optimal δ and σ values are determined.展开更多
基金Beijing Natural Science Foundation,Grant/Award Number:L222145 and L222030Emerging Engineering Interdisciplinary Project and the Fundamental Research Funds for the Central Universities,Grant/Award Number:PKU2022XGK008Peking University Medicine Fund of Fostering Young Scholars’Scientific&Technological Innovation,Grant/Award Number:BMU2022PY010。
文摘Background:Osteoporosis is a chronic bone disease characterized by bone loss and decreased bone strength.However,current anti-resorptive drugs carry a risk of various complications.The deep learning-based efficacy prediction system(DLEPS)is a forecasting tool that can effectively compete in drug screening and prediction based on gene expression changes.This study aimed to explore the protective effect and potential mechanisms of cinobufotalin(CB),a traditional Chinese medicine(TCM),on bone loss.Methods:DLEPS was employed for screening anti-osteoporotic agents according to gene profile changes in primary osteoporosis.Micro-CT,histological and morphological analysis were applied for the bone protective detection of CB,and the osteogenic differentiation/function in human bone marrow mesenchymal stem cells(hBMMSCs)were also investigated.The underlying mechanism was verified using qRT-PCR,Western blot(WB),immunofluorescence(IF),etc.Results:A safe concentration(0.25mg/kg in vivo,0.05μM in vitro)of CB could effectively preserve bone mass in estrogen deficiency-induced bone loss and promote osteogenic differentiation/function of hBMMSCs.Both BMPs/SMAD and Wnt/β-catenin signaling pathways participated in CB-induced osteogenic differentiation,further regulating the expression of osteogenesis-associated factors,and ultimately promoting osteogenesis.Conclusion:Our study demonstrated that CB could significantly reverse estrogen deficiency-induced bone loss,further promoting osteogenic differentiation/function of hBMMSCs,with BMPs/SMAD and Wnt/β-catenin signaling pathways involved.
文摘The presence of a large palatal or maxillary defect after partial or total maxillectomy for tumor, trauma or congenital deformation poses a challenge to prosthodontists, particularly when the use of an implant cannot be considered. This case report described the use of an air valve in a hollow silicone obturator to manufacture an inflatable obturator that could be extended further into undercut area to retain itself. The inflatable obturator exhibited adequate retention, stability and border sealing, thereby improving the masticatory, pronunciation and swallowing functions of patients. It may be a suitable alternative treatment option to an implant-retained obturator.
基金This work was supported by the Fundamental Research Fund for Central Universities(Grant No.JZ2015HGBZ0129)the Open End Fund of Jiangsu University(Grant No.NRCP201603).
文摘The internal flow field of an axial-flow pump under low flow rate conditions is extremely turbulent. The unstable flow region is formed due to the tip leakage and the rotating stall, and is distinguished by a saddle patterned zone in its flow-head curve that demonstrates restrictions in its workable flow range. It is therefore important to understand and improve the operational characteristics of an axial-flow pump under low flow rate conditions. In this study, experiments are performed for the performances of an axial-flow pump at the flow rate in a range between 0.8Qd and 1.2Qd, with the internal flow field measured by the particle image velocimetry (PIV), and with the pump performances and the internal flow field simulated by the Ansys CFX. The simulation results agree well with the experimental results. From the predicted heads at the flow rate in the range between 0.1Qd and 0.7Qd by the numerical simulation, the complete flow-head curves are obtained. The streamlines and the velocity contours in the region in front of the impeller leading edge under different flow conditions are analyzed. By adopting the double-inlet structures, the flow-head characteristics are studied by varying the values of δ and σ respectively, where δ denotes the gap between the inner cylinder end and the impeller leading edge, and σ denotes the gap between the inner cylinder and the outer cylinder. The findings indicate that with the double-inlet design, the velocity distribution can be effectively improved in the region in front of the impeller leading edge, as well as the head performance under the low flow rate conditions, and the positive slope value of the head curve is reduced in the unstable flow range. The optimal δ and σ values are determined.
