Seedlings of Taxus chinensis var.mairei were used as experimental materials to study the adaptation of Piriformospora indica to this plant under water stress.The materials were divided into two groups,namely,with or w...Seedlings of Taxus chinensis var.mairei were used as experimental materials to study the adaptation of Piriformospora indica to this plant under water stress.The materials were divided into two groups,namely,with or without inoculation with P.indica.Each group was subjected to four different levels of water stress.Vitality and physiological and biochemical indexes of the roots of T.chinensis var.mairei were regularly measured.Under water stress,T.chinensis var.mairei had significantly decreased root vitality;root vitality was higher in inoculated roots than in uninoculated roots.Under intense water stress,the inoculated roots had a higher soluble sugar content than the uninoculated roots.Under water stress,T.chinensis var.mairei experienced decreased activity of aerobic respiratory metabolic enzymes.The activity of anaerobic respiratory metabolic enzymes and alcohol dehydrogenase initially increased and then decreased,whereas that of lactate dehydrogenase increased.The inoculated roots had a higher activity of respiratory metabolic enzymes than the uninoculated roots.As water stress was further intensified,the roots had significantly decreased activity of aerobic respiratory metabolic enzymes and significantly increased activity of anaerobic respiratory metabolic enzymes.The activity of respiratory metabolic enzymes decreased faster in the uninoculated roots than in the inoculated roots.This study demonstrated that Piriformospora indica plays a positive role in enhancing the antihypoxic ability of T.chinensis var.mairei,thereby alleviating plant damage due to water stress.展开更多
Drought stress negatively impacts growth and physiological processes in plants.The foliar application of glycine betaine(GB)is an effective and low-cost approach to improve the drought tolerance of trees.This study ex...Drought stress negatively impacts growth and physiological processes in plants.The foliar application of glycine betaine(GB)is an effective and low-cost approach to improve the drought tolerance of trees.This study examined the effect of exogenously applied GB on the cell membrane permeability,osmotic adjustment,and antioxidant enzyme activities of Phoebe hunanensis Hand.-Mazz under drought stress.Two levels(0 and 800 mL)of water irrigation were tested under different applied GB concentrations(0,50,100,and 200 mM).Drought stress decreased the relative water content by 58.5%while increased the electric conductivity,malondialdehyde,proline,soluble proteins,soluble sugars,and antioxidant enzyme activities(superoxide dismutase,catalase,peroxidase)by up to 62.9%,42.4%,87.0%,19.1%,60.5%,68.3%,71.7%,and 83.8%,respectively,on the 25^(th) day.The foliar application of GB,especially at 100 mM,increased the relative water content of P.hunanensis leaves under drought stress.The concentration of GB from 50 to 100 mM effectively alleviated the improvement of cell membrane permeability and inhibited the accumulation of membrane lipid peroxidation products.Under drought stress,the concentrations of proline,soluble proteins,and soluble sugars in the leaves of P.hunanensis increased as the applied GB concentration was increased and the water stress time was prolonged.Exogenously applied GB decreased oxidative stress and improved antioxidant enzyme activities as compared with treatments without GB application.Furthermore,the physiological and biochemical indexes of P.hunanensis showed a certain dose effect on exogenous GB concentration.These results suggest that GB helps maintain the drought tolerance of P.hunanensis.展开更多
Four different ratios of river sand,ceramic pellets,vermiculite and perlite(1:1),and field soil were selected as the substrates in this experiment,and four gradient levels of root waterlogging,half waterlogging,full w...Four different ratios of river sand,ceramic pellets,vermiculite and perlite(1:1),and field soil were selected as the substrates in this experiment,and four gradient levels of root waterlogging,half waterlogging,full waterlogging and normal were set to investigate the effects of different gradients of waterlogging stress on the root morphology of Taxus chinensis var.mairei seedlings under different substrates.In this study,the root anatomical structure of Taxus chinensis var.mairei under different waterlogging stress was observed by the paraffin section method.The roots of T.chinensis var.mairei were diarch,with no pith and resin canals.There was a large number of tannins in the pericycle of the aerial adventitious roots of seedlings adapted to waterlogging.Also,the endodermis has obvious casparian strip thickening,and there were 4-5 layers of large parenchymatous cells in the close to the inner side of the pericycle in the vascular cylinder,which could increase the storage capacity,and transport capacity of the root.Under the treatment of root waterlogging stress,the development of plant roots in the mixed substrate of vermiculite and,perlite was the earliest.Under half waterlogging stress,T.chinensis var.mairei seedlings treated with various substrates all could better adapt to the environment of waterlogging stress.Under the stress of fully waterlogging,the roots of seedlings planted in river sand substrate developed secondary growth.展开更多
[Objectives]This study was conducted to evaluate the value of local Brassicaceae potherb resources in Shiyan City.[Methods]Seven species of local Brassicaceae edible plants resources in Shiyan City,including Cardamine...[Objectives]This study was conducted to evaluate the value of local Brassicaceae potherb resources in Shiyan City.[Methods]Seven species of local Brassicaceae edible plants resources in Shiyan City,including Cardamine hirsuta L.,Cardamine flexuosa,Cardamine impatiens L.,Capsella bursa-pastoris(Linn.),Orychophragmus violaceus(L.)O.E.Schulz,Draba nemorosa L.,Rorippa indica(L.)Hiern.,were investigated using sampling method,and their value was evaluated.[Results]①Shiyan City was rich in resources of local Brassicaceae potherbs,mostly of which grew in humid areas.②In the communities of target species,there were 20 species of plants in 19 genera of 15 families in the arbor layer,19 species of plants in 16 genera from 15 families in the shrub layer,and 58 species of plants in 51 genera from 23 families in the herbaceous layer.The tree,shrub and herb species with the highest dominance Populus simonii var.przewalskii,Robinia pseudoacacia and Chrysanthemum indicum.Local Brassicaceae potherbs in Shiyan City have high edible and medicinal value,especially Cardamine,which has strong selenium-rich ability and is worthy of vigorous development.[Conclusions]This study provides scientific basis and reference for the development and utilization of local potherbs of Brassicaceae in this area.展开更多
[Objectives]This study was conducted to improve the ecological and economic benefits of Pinus elliottii plantations in Pengchang Forest Farm, Jingmen City. [Methods] P. elliottii plantations in Pengchang Forest Farm i...[Objectives]This study was conducted to improve the ecological and economic benefits of Pinus elliottii plantations in Pengchang Forest Farm, Jingmen City. [Methods] P. elliottii plantations in Pengchang Forest Farm in Jingmen City were selected as the research object, and the appearance and quantitative characteristics of P. elliottii community were studied and analyzed by a sampling method. [Results] Phanerophytes were the most in the P. elliottii community, accounting for 50%. In the community, except P. elliottii, the importance values of native plants were higher. The order of species diversity of P. elliottii community in Pengchang Forest Farm was herb layer, tree layer and shrub layer. The stress resistance of the community was weak, and strong intraspecific competition existed in P. elliottii. It is recommended to thin P. elliottii trees with insufficient lighting, poor growth potential and severely damaged tree body, and interplant Phoebe zhennan after thinning the P. elliottii plantations, so as to create a coniferous and broad-leaved evergreen mixed forest. [Conclusions] This study provides scientific support for thinning, tending, and forest management of P. elliottii plantations.展开更多
In this paper,we conduct field investigation and statistical analysis on the plant species of Binjiang Park in Jingzhou City,study the plant landscaping techniques,analyze the current situation of plant arrangement,an...In this paper,we conduct field investigation and statistical analysis on the plant species of Binjiang Park in Jingzhou City,study the plant landscaping techniques,analyze the current situation of plant arrangement,and put forward the corresponding plant landscaping strategies and plant arrangement recommendations.展开更多
Transplantation experiment was carried out using biennial container seedlings and bare root seedlings of Phoebe zhennan S. Lee as materials. In the experiment,it measured the growth indicators such as the seedling hei...Transplantation experiment was carried out using biennial container seedlings and bare root seedlings of Phoebe zhennan S. Lee as materials. In the experiment,it measured the growth indicators such as the seedling height,basal diameter,and number of branches and leaves. Besides,it also measured net photosynthetic rate( Pn),transpiration rate( Tr),stomatal conductance( Gs),intercellular CO_2 concentration( Ci) and soluble sugar,soluble protein,malondialdehyde( MDA) content,superoxide dismutase activity and root activity. The results indicated that the growth indicators of the Phoebe container seedlings performed the best in the plant height,basal diameter,and number of branches and leaves; photosynthetic capacity was the strongest,and the fertilization could enhance the photosynthetic capacity of seedlings; soluble sugar,soluble protein content,superoxide dismutase activity and root activity of Phoebe container seedlings were higher than that of bare root seedlings.展开更多
In this paper,the geographical distribution,community characteristics and DBH class structure of Picea neoveitchii Mast. population were investigated and analyzed by systematical and ecological approaches. In addition...In this paper,the geographical distribution,community characteristics and DBH class structure of Picea neoveitchii Mast. population were investigated and analyzed by systematical and ecological approaches. In addition,the endangered mechanism and the protection measures were put forward by analyzing the Picea neoveitchii Mast. resource distribution in Hubei Province to provide effective scientific basis for further research. The results showed that Picea neoveitchii Mast. was found in Baokang,Enshi,Shennongjia and Zhuxi of Hubei Province,there were 9 distribution points and only a wild forest was found in Baokang. The community of Baokang County was not rich in species composition and 32 species,29 genera and 21 families were examined. Meanwhile,temperate zone was the main flora element of this community,the phaenerophytes plant was most dominant and there were few hemicryptophytes and it lacked therophytes. In this community,Picea neoveitchii Mast. was in a dominant position,including lots of treelets,so the age structure of the population was growing.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.31270740).
文摘Seedlings of Taxus chinensis var.mairei were used as experimental materials to study the adaptation of Piriformospora indica to this plant under water stress.The materials were divided into two groups,namely,with or without inoculation with P.indica.Each group was subjected to four different levels of water stress.Vitality and physiological and biochemical indexes of the roots of T.chinensis var.mairei were regularly measured.Under water stress,T.chinensis var.mairei had significantly decreased root vitality;root vitality was higher in inoculated roots than in uninoculated roots.Under intense water stress,the inoculated roots had a higher soluble sugar content than the uninoculated roots.Under water stress,T.chinensis var.mairei experienced decreased activity of aerobic respiratory metabolic enzymes.The activity of anaerobic respiratory metabolic enzymes and alcohol dehydrogenase initially increased and then decreased,whereas that of lactate dehydrogenase increased.The inoculated roots had a higher activity of respiratory metabolic enzymes than the uninoculated roots.As water stress was further intensified,the roots had significantly decreased activity of aerobic respiratory metabolic enzymes and significantly increased activity of anaerobic respiratory metabolic enzymes.The activity of respiratory metabolic enzymes decreased faster in the uninoculated roots than in the inoculated roots.This study demonstrated that Piriformospora indica plays a positive role in enhancing the antihypoxic ability of T.chinensis var.mairei,thereby alleviating plant damage due to water stress.
基金This work was supported by the Science and Technology Research Project of Hubei Provincial Department of Education(Q20191309)the Engineering Research Center of Ecology and Agricultural Use of Wetland(Ministry of Education,KF2018113)Shuangzhu Forest Farm in Zhuxi County(2020H21003).
文摘Drought stress negatively impacts growth and physiological processes in plants.The foliar application of glycine betaine(GB)is an effective and low-cost approach to improve the drought tolerance of trees.This study examined the effect of exogenously applied GB on the cell membrane permeability,osmotic adjustment,and antioxidant enzyme activities of Phoebe hunanensis Hand.-Mazz under drought stress.Two levels(0 and 800 mL)of water irrigation were tested under different applied GB concentrations(0,50,100,and 200 mM).Drought stress decreased the relative water content by 58.5%while increased the electric conductivity,malondialdehyde,proline,soluble proteins,soluble sugars,and antioxidant enzyme activities(superoxide dismutase,catalase,peroxidase)by up to 62.9%,42.4%,87.0%,19.1%,60.5%,68.3%,71.7%,and 83.8%,respectively,on the 25^(th) day.The foliar application of GB,especially at 100 mM,increased the relative water content of P.hunanensis leaves under drought stress.The concentration of GB from 50 to 100 mM effectively alleviated the improvement of cell membrane permeability and inhibited the accumulation of membrane lipid peroxidation products.Under drought stress,the concentrations of proline,soluble proteins,and soluble sugars in the leaves of P.hunanensis increased as the applied GB concentration was increased and the water stress time was prolonged.Exogenously applied GB decreased oxidative stress and improved antioxidant enzyme activities as compared with treatments without GB application.Furthermore,the physiological and biochemical indexes of P.hunanensis showed a certain dose effect on exogenous GB concentration.These results suggest that GB helps maintain the drought tolerance of P.hunanensis.
基金This work was supported by the National Natural Science Foundation of China[Grant No.31270740].
文摘Four different ratios of river sand,ceramic pellets,vermiculite and perlite(1:1),and field soil were selected as the substrates in this experiment,and four gradient levels of root waterlogging,half waterlogging,full waterlogging and normal were set to investigate the effects of different gradients of waterlogging stress on the root morphology of Taxus chinensis var.mairei seedlings under different substrates.In this study,the root anatomical structure of Taxus chinensis var.mairei under different waterlogging stress was observed by the paraffin section method.The roots of T.chinensis var.mairei were diarch,with no pith and resin canals.There was a large number of tannins in the pericycle of the aerial adventitious roots of seedlings adapted to waterlogging.Also,the endodermis has obvious casparian strip thickening,and there were 4-5 layers of large parenchymatous cells in the close to the inner side of the pericycle in the vascular cylinder,which could increase the storage capacity,and transport capacity of the root.Under the treatment of root waterlogging stress,the development of plant roots in the mixed substrate of vermiculite and,perlite was the earliest.Under half waterlogging stress,T.chinensis var.mairei seedlings treated with various substrates all could better adapt to the environment of waterlogging stress.Under the stress of fully waterlogging,the roots of seedlings planted in river sand substrate developed secondary growth.
基金Hubei Provincial Technological Innovation Special Major Project(2019ABA113)Wuhan Polytechnic University-Deyuan Health Industry Group Academician Expert Workstation Horizontal Project(whpu-2019-cg-138,whpu-2018-cg-041,whpu-2018-cg-140).
文摘[Objectives]This study was conducted to evaluate the value of local Brassicaceae potherb resources in Shiyan City.[Methods]Seven species of local Brassicaceae edible plants resources in Shiyan City,including Cardamine hirsuta L.,Cardamine flexuosa,Cardamine impatiens L.,Capsella bursa-pastoris(Linn.),Orychophragmus violaceus(L.)O.E.Schulz,Draba nemorosa L.,Rorippa indica(L.)Hiern.,were investigated using sampling method,and their value was evaluated.[Results]①Shiyan City was rich in resources of local Brassicaceae potherbs,mostly of which grew in humid areas.②In the communities of target species,there were 20 species of plants in 19 genera of 15 families in the arbor layer,19 species of plants in 16 genera from 15 families in the shrub layer,and 58 species of plants in 51 genera from 23 families in the herbaceous layer.The tree,shrub and herb species with the highest dominance Populus simonii var.przewalskii,Robinia pseudoacacia and Chrysanthemum indicum.Local Brassicaceae potherbs in Shiyan City have high edible and medicinal value,especially Cardamine,which has strong selenium-rich ability and is worthy of vigorous development.[Conclusions]This study provides scientific basis and reference for the development and utilization of local potherbs of Brassicaceae in this area.
文摘[Objectives]This study was conducted to improve the ecological and economic benefits of Pinus elliottii plantations in Pengchang Forest Farm, Jingmen City. [Methods] P. elliottii plantations in Pengchang Forest Farm in Jingmen City were selected as the research object, and the appearance and quantitative characteristics of P. elliottii community were studied and analyzed by a sampling method. [Results] Phanerophytes were the most in the P. elliottii community, accounting for 50%. In the community, except P. elliottii, the importance values of native plants were higher. The order of species diversity of P. elliottii community in Pengchang Forest Farm was herb layer, tree layer and shrub layer. The stress resistance of the community was weak, and strong intraspecific competition existed in P. elliottii. It is recommended to thin P. elliottii trees with insufficient lighting, poor growth potential and severely damaged tree body, and interplant Phoebe zhennan after thinning the P. elliottii plantations, so as to create a coniferous and broad-leaved evergreen mixed forest. [Conclusions] This study provides scientific support for thinning, tending, and forest management of P. elliottii plantations.
文摘In this paper,we conduct field investigation and statistical analysis on the plant species of Binjiang Park in Jingzhou City,study the plant landscaping techniques,analyze the current situation of plant arrangement,and put forward the corresponding plant landscaping strategies and plant arrangement recommendations.
基金Supported by Key Technology R&D Program of Hubei Province(2013BBB24)Second Survey of Key Wild Plant Resources under Protection in China"Special Survey of Phoebe zhennan S.Lee Resources in Hubei Province"
文摘Transplantation experiment was carried out using biennial container seedlings and bare root seedlings of Phoebe zhennan S. Lee as materials. In the experiment,it measured the growth indicators such as the seedling height,basal diameter,and number of branches and leaves. Besides,it also measured net photosynthetic rate( Pn),transpiration rate( Tr),stomatal conductance( Gs),intercellular CO_2 concentration( Ci) and soluble sugar,soluble protein,malondialdehyde( MDA) content,superoxide dismutase activity and root activity. The results indicated that the growth indicators of the Phoebe container seedlings performed the best in the plant height,basal diameter,and number of branches and leaves; photosynthetic capacity was the strongest,and the fertilization could enhance the photosynthetic capacity of seedlings; soluble sugar,soluble protein content,superoxide dismutase activity and root activity of Phoebe container seedlings were higher than that of bare root seedlings.
基金Supported By the Second National Investigation of Key Wild Plant Resources-Special Investigation of Picea neoveitchii Mast.Resources in Hubei Province
文摘In this paper,the geographical distribution,community characteristics and DBH class structure of Picea neoveitchii Mast. population were investigated and analyzed by systematical and ecological approaches. In addition,the endangered mechanism and the protection measures were put forward by analyzing the Picea neoveitchii Mast. resource distribution in Hubei Province to provide effective scientific basis for further research. The results showed that Picea neoveitchii Mast. was found in Baokang,Enshi,Shennongjia and Zhuxi of Hubei Province,there were 9 distribution points and only a wild forest was found in Baokang. The community of Baokang County was not rich in species composition and 32 species,29 genera and 21 families were examined. Meanwhile,temperate zone was the main flora element of this community,the phaenerophytes plant was most dominant and there were few hemicryptophytes and it lacked therophytes. In this community,Picea neoveitchii Mast. was in a dominant position,including lots of treelets,so the age structure of the population was growing.
