Volumetric muscle loss(VML)refers to a composite,en bloc loss of skeletal muscle mass resulting in functional impairment.These injuries normally heal with excessive fibrosis,minimal skeletal muscle regeneration,and po...Volumetric muscle loss(VML)refers to a composite,en bloc loss of skeletal muscle mass resulting in functional impairment.These injuries normally heal with excessive fibrosis,minimal skeletal muscle regeneration,and poor functional recovery.Functional muscle transfer is a treatment option for some patients but is limited both by the degree of functional restoration as well as donor site morbidity.As such,new therapeutic options are necessary.De novo regeneration of skeletal muscle,by way of tissue engineering,is an emerging strategy to treat VML.This review evaluates available scaffolds for promoting skeletal muscle regeneration and functional recovery following VML.The use of growth factors and stem cell therapies,which may augment scaffold integration and skeletal muscle reconstitution,are also discussed.Regenerative medicine with the use of scaffolds is a promising area in skeletal muscle reconstruction and VML treatment.展开更多
Acute and chronic wounds affect millions of people around the world,imposing a growing financial burden on patients and hospitals.Despite the application of current wound management strategies,the physiological healin...Acute and chronic wounds affect millions of people around the world,imposing a growing financial burden on patients and hospitals.Despite the application of current wound management strategies,the physiological healing process is disrupted in many cases,resulting in impaired wound healing.Therefore,more efficient and easy-to-use treatment modalities are needed.In this study,we demonstrate the benefit of in vivo printed,growth factor-eluting adhesive scaffolds for the treatment of full-thickness wounds in a porcine model.A custom-made handheld printer is implemented to finely print gelatin-methacryloyl(GelMA)hydrogel containing vascular endothelial growth factor(VEGF)into the wounds.In vitro and in vivo results show that the in situ GelMA crosslinking induces a strong scaffold adhesion and enables printing on curved surfaces of wet tissues,without the need for any sutures.The scaffold is further shown to offer a sustained release of VEGF,enhancing the migration of endothelial cells in vitro.Histological analyses demonstrate that the administration of the VEGF-eluting GelMA scaffolds that remain adherent to the wound bed significantly improves the quality of healing in porcine wounds.The introduced in vivo printing strategy for wound healing applications is translational and convenient to use in any place,such as an operating room,and does not require expensive bioprinters or imaging modalities.展开更多
文摘Volumetric muscle loss(VML)refers to a composite,en bloc loss of skeletal muscle mass resulting in functional impairment.These injuries normally heal with excessive fibrosis,minimal skeletal muscle regeneration,and poor functional recovery.Functional muscle transfer is a treatment option for some patients but is limited both by the degree of functional restoration as well as donor site morbidity.As such,new therapeutic options are necessary.De novo regeneration of skeletal muscle,by way of tissue engineering,is an emerging strategy to treat VML.This review evaluates available scaffolds for promoting skeletal muscle regeneration and functional recovery following VML.The use of growth factors and stem cell therapies,which may augment scaffold integration and skeletal muscle reconstitution,are also discussed.Regenerative medicine with the use of scaffolds is a promising area in skeletal muscle reconstruction and VML treatment.
基金The financial support from the National Institutes of Health(GM126831,AR073822)Stepping Strong Innovator Award are gratefully acknowledged.
文摘Acute and chronic wounds affect millions of people around the world,imposing a growing financial burden on patients and hospitals.Despite the application of current wound management strategies,the physiological healing process is disrupted in many cases,resulting in impaired wound healing.Therefore,more efficient and easy-to-use treatment modalities are needed.In this study,we demonstrate the benefit of in vivo printed,growth factor-eluting adhesive scaffolds for the treatment of full-thickness wounds in a porcine model.A custom-made handheld printer is implemented to finely print gelatin-methacryloyl(GelMA)hydrogel containing vascular endothelial growth factor(VEGF)into the wounds.In vitro and in vivo results show that the in situ GelMA crosslinking induces a strong scaffold adhesion and enables printing on curved surfaces of wet tissues,without the need for any sutures.The scaffold is further shown to offer a sustained release of VEGF,enhancing the migration of endothelial cells in vitro.Histological analyses demonstrate that the administration of the VEGF-eluting GelMA scaffolds that remain adherent to the wound bed significantly improves the quality of healing in porcine wounds.The introduced in vivo printing strategy for wound healing applications is translational and convenient to use in any place,such as an operating room,and does not require expensive bioprinters or imaging modalities.
