[ Objective] This study aimed to compare the effects of different processing methods on the content of chlorogenie acid in Fructus Garden/ae. [ Method] The chromatographic separation was performed on an Inersil ODS-2 ...[ Objective] This study aimed to compare the effects of different processing methods on the content of chlorogenie acid in Fructus Garden/ae. [ Method] The chromatographic separation was performed on an Inersil ODS-2 C18 column （250 mm× 4.6 mm, 5 μm） with acetonitrile -0. 1% acetic acid solution （ 12： 88, V/V） as the mobile phase under conditions of tlow rate 1.0 mb/min, detection wavelength 327 ran, column temperature 25℃. [ Result J With a range of 0.42-2.52 μg/i.d, ehlorogenic acid concentration exhibited a good linear relationship with peak area （n =6, R =0. 999 9）. The average recovery rate was 100. 83%, RSD was 1.30%. [Conclusion] The established method is eonveniem, rapid and accurate with good repeatability, which can be used to effectively control the quality of different orocessed oroduets of Fructus C.ardeniae.展开更多
文摘[ Objective] This study aimed to compare the effects of different processing methods on the content of chlorogenie acid in Fructus Garden/ae. [ Method] The chromatographic separation was performed on an Inersil ODS-2 C18 column （250 mm× 4.6 mm, 5 μm） with acetonitrile -0. 1% acetic acid solution （ 12： 88, V/V） as the mobile phase under conditions of tlow rate 1.0 mb/min, detection wavelength 327 ran, column temperature 25℃. [ Result J With a range of 0.42-2.52 μg/i.d, ehlorogenic acid concentration exhibited a good linear relationship with peak area （n =6, R =0. 999 9）. The average recovery rate was 100. 83%, RSD was 1.30%. [Conclusion] The established method is eonveniem, rapid and accurate with good repeatability, which can be used to effectively control the quality of different orocessed oroduets of Fructus C.ardeniae.
