The aim of this study was to investigate antibacterial activity of Origanum compactum essential oils collected at three phenological stages on Escherichia coli and Bacillus subtilis. The antibacterial activity was eva...The aim of this study was to investigate antibacterial activity of Origanum compactum essential oils collected at three phenological stages on Escherichia coli and Bacillus subtilis. The antibacterial activity was evaluated using the agar-well diffusion assay. The MIC and MBC values were determined using the micro-dilution assay. The investigation of the antibacterial action was carried out by the evaluation of the effect of O. compactum essential oils on the antibacterial kinetic growth, the integrity of cell membrane and permeability of the cell membrane. The anti-quorum sensing activity was tested by the inhibition of the biofilm formation. The findings of this study showed that O. compactum essential oil has potent antibacterial activities against E. coli and B. subtilis. The lowest inhibition value against B. subtilis was obtained with O. compactum essential oil at the post-flowering stage (MIC=MBC=0.0312%(v/v)). The antibacterial mechanisms of O. compactum essential oils are related to the disturbing of the cell membrane integrity and the increasing of the membrane permeability, which leads to the leakage of genetic materials (DNA and RNA). Moreover, O. compactum essential oils inhibited the formation of the biofilms, a phenotype that has been known to be quorum sensing regulated.展开更多
Traditional treatment of infectious diseases is based on compounds that aim to kill or inhibit bacterial growth. The bacterial resistance against antibiotics is a serious issue for public health. Today, new therapeuti...Traditional treatment of infectious diseases is based on compounds that aim to kill or inhibit bacterial growth. The bacterial resistance against antibiotics is a serious issue for public health. Today, new therapeutic targets other than the bacterial wall were deciphered. Quorum sensing or bacterial pheromones are molecules called auto-inducer secreted by bacteria to regulate some functions such as antibiotic resistance and biofilms formation. This therapeutic target is well-studied worldwide, nevertheless the scientific data are not updated and only recent researches started to look into its potential as a target to fight against infectious diseases. A major concern with this approach is the frequently observed development of resistance to antimicrobial compounds. Therefore, this paper aims to provide a current overview of the quorum sensing system in bacteria by revealing their implication in biofilms formation and the development of antibiotic resistance, and an update on their importance as a potential target for natural substances.展开更多
Moroccan medicinal plants exhibit several pharmacological properties such as antimicrobial,anticancer,antidiabetic,analgesic,and anti-inflammatory effects,which are related to the presence of numerous bioactive compou...Moroccan medicinal plants exhibit several pharmacological properties such as antimicrobial,anticancer,antidiabetic,analgesic,and anti-inflammatory effects,which are related to the presence of numerous bioactive compounds,including phenolic acids,flavonoids,and terpenoids.In the present review,we systematically evaluate previously published reports on the anti-inflammatory and analgesic effects of Moroccan medicinal plants.The in vitro investigations revealed that Moroccan medicinal plants inhibit several enzymes related to inflammatory processes,whereas in vivo studies noted significant anti-inflammatory and analgesic effects as demonstrated using different experimental models.Various bioactive compounds exhibiting in vitro and in vivo anti-inflammatory and analgesic effects,with diverse mechanisms of action,have been identified.Some plants and their bioactive compounds reveal specific secondary metabolites that possess important anti-inflammatory effects in clinical investigations.Our review proposes the potential applications of Moroccan medicinal plants as sources of anti-inflammatory and analgesic agents.展开更多
Objective: To determin the chemical compounds of Mentha suaveolens(M. suaveolens) and Pinus halepensis(P. halepensis) essential oils(Eos) and evaluate their antioxidant and antibacterial activities. Methods: The chemi...Objective: To determin the chemical compounds of Mentha suaveolens(M. suaveolens) and Pinus halepensis(P. halepensis) essential oils(Eos) and evaluate their antioxidant and antibacterial activities. Methods: The chemical composition of P. halepensis and M. suaveolens EOs was determined by GC-MS analysis. The antioxidant activity was evaluated using DPPH,ABTS and FRAP assays. The antibacterial effect was tested against 6 bacterial strains using the well diffusion method and micro-dilution assay. Results: The major components of P. halepensis EOs were β-caryophyllene(28.04%),myrcene(23.81%) and α-pinene(12.02%). However,piperitenone oxid(56.28%),piperitenone(11.64%) and pulegone(6.16%) were the major components of M. suaveolens EOs. M. suaveolens EOs showed remarkable antioxidant activities compared with P. halepensis EOs,showing antioxidant capacity values of IC50=(64.76±2.24) μg/mL,IC50=(82.73±3.34) μg/mL,and IC50=(93.35±4.45) μg/mL,revealed by DPPH,ABTS and FRAP assays,respectively. However,P. halepensis EOs showed interesting antibacterial effects against all bacterial strains. The most sensible strains to P. halepensis EOs were Staphylococcus aureus [(34.00±0.50) mm],Listeria monocytogenes [(31.00±1.50)] mm and Proteus mirabilis [(29.00±2.25)mm]. Furthermore,the lowest minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) values were revealed by P. halepensis EOs against Staphylococcus aureus [MIC=MBC=0.125%(v/v)] and Listeria monocytogenes [MIC=MBC=0.25%(v/v)]. Conclusions: P. halepensis and M. suaveolens EOs contain bioactive compounds that could have potential applications against bacterial infections and oxidative stress related diseases as well as for food conservation. However,further investigations are necessary to isolate and investigate the action mechanisms of these bioactive compounds.展开更多
基金the "Centre National pour la Recherche Scientifique et Technique" (CNRST) "Agence Nationale des Plantes Medicinales et Aromatiques" (ANPMA) for their funding supports
文摘The aim of this study was to investigate antibacterial activity of Origanum compactum essential oils collected at three phenological stages on Escherichia coli and Bacillus subtilis. The antibacterial activity was evaluated using the agar-well diffusion assay. The MIC and MBC values were determined using the micro-dilution assay. The investigation of the antibacterial action was carried out by the evaluation of the effect of O. compactum essential oils on the antibacterial kinetic growth, the integrity of cell membrane and permeability of the cell membrane. The anti-quorum sensing activity was tested by the inhibition of the biofilm formation. The findings of this study showed that O. compactum essential oil has potent antibacterial activities against E. coli and B. subtilis. The lowest inhibition value against B. subtilis was obtained with O. compactum essential oil at the post-flowering stage (MIC=MBC=0.0312%(v/v)). The antibacterial mechanisms of O. compactum essential oils are related to the disturbing of the cell membrane integrity and the increasing of the membrane permeability, which leads to the leakage of genetic materials (DNA and RNA). Moreover, O. compactum essential oils inhibited the formation of the biofilms, a phenotype that has been known to be quorum sensing regulated.
文摘Traditional treatment of infectious diseases is based on compounds that aim to kill or inhibit bacterial growth. The bacterial resistance against antibiotics is a serious issue for public health. Today, new therapeutic targets other than the bacterial wall were deciphered. Quorum sensing or bacterial pheromones are molecules called auto-inducer secreted by bacteria to regulate some functions such as antibiotic resistance and biofilms formation. This therapeutic target is well-studied worldwide, nevertheless the scientific data are not updated and only recent researches started to look into its potential as a target to fight against infectious diseases. A major concern with this approach is the frequently observed development of resistance to antimicrobial compounds. Therefore, this paper aims to provide a current overview of the quorum sensing system in bacteria by revealing their implication in biofilms formation and the development of antibiotic resistance, and an update on their importance as a potential target for natural substances.
基金the Centre National pour la Recherche Scientifique et Technique (CNRST)l’Agence Nationale des Plantes Médicinales et Aromatiques (ANPMA) for supporitng this study (Grant No.: PMA2019/1)
文摘Moroccan medicinal plants exhibit several pharmacological properties such as antimicrobial,anticancer,antidiabetic,analgesic,and anti-inflammatory effects,which are related to the presence of numerous bioactive compounds,including phenolic acids,flavonoids,and terpenoids.In the present review,we systematically evaluate previously published reports on the anti-inflammatory and analgesic effects of Moroccan medicinal plants.The in vitro investigations revealed that Moroccan medicinal plants inhibit several enzymes related to inflammatory processes,whereas in vivo studies noted significant anti-inflammatory and analgesic effects as demonstrated using different experimental models.Various bioactive compounds exhibiting in vitro and in vivo anti-inflammatory and analgesic effects,with diverse mechanisms of action,have been identified.Some plants and their bioactive compounds reveal specific secondary metabolites that possess important anti-inflammatory effects in clinical investigations.Our review proposes the potential applications of Moroccan medicinal plants as sources of anti-inflammatory and analgesic agents.
基金supported by le Centre National pour la Recherche Scientifique et Technique et l’Agence Nationale des Plantes Médicinales et Aromatiques
文摘Objective: To determin the chemical compounds of Mentha suaveolens(M. suaveolens) and Pinus halepensis(P. halepensis) essential oils(Eos) and evaluate their antioxidant and antibacterial activities. Methods: The chemical composition of P. halepensis and M. suaveolens EOs was determined by GC-MS analysis. The antioxidant activity was evaluated using DPPH,ABTS and FRAP assays. The antibacterial effect was tested against 6 bacterial strains using the well diffusion method and micro-dilution assay. Results: The major components of P. halepensis EOs were β-caryophyllene(28.04%),myrcene(23.81%) and α-pinene(12.02%). However,piperitenone oxid(56.28%),piperitenone(11.64%) and pulegone(6.16%) were the major components of M. suaveolens EOs. M. suaveolens EOs showed remarkable antioxidant activities compared with P. halepensis EOs,showing antioxidant capacity values of IC50=(64.76±2.24) μg/mL,IC50=(82.73±3.34) μg/mL,and IC50=(93.35±4.45) μg/mL,revealed by DPPH,ABTS and FRAP assays,respectively. However,P. halepensis EOs showed interesting antibacterial effects against all bacterial strains. The most sensible strains to P. halepensis EOs were Staphylococcus aureus [(34.00±0.50) mm],Listeria monocytogenes [(31.00±1.50)] mm and Proteus mirabilis [(29.00±2.25)mm]. Furthermore,the lowest minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) values were revealed by P. halepensis EOs against Staphylococcus aureus [MIC=MBC=0.125%(v/v)] and Listeria monocytogenes [MIC=MBC=0.25%(v/v)]. Conclusions: P. halepensis and M. suaveolens EOs contain bioactive compounds that could have potential applications against bacterial infections and oxidative stress related diseases as well as for food conservation. However,further investigations are necessary to isolate and investigate the action mechanisms of these bioactive compounds.
