The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic mac...The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic macular edema are anti-vascular endothelial growth factor drugs and laser photocoagulation.However,although the macular thickness can be normalized with each of these two therapies used alone,the vision does not improve in many patients.This might result from the incomplete recovery of retinal ganglion cell injury.Therefore,a prospective,non-randomized,controlled clinical trial was designed to investigate the effect of anti-vascular endothelial growth factor drugs combined with laser photocoagulation on the integrity of retinal ganglion cells in patients with diabetic macular edema and its relationship with vision recovery.In this trial,150 patients with diabetic macular edema will be equally divided into three groups according to therapeutic methods,followed by treatment with anti-vascular endothelial growth factor drugs,laser photocoagulation therapy,and their combination.All patients will be followed up for 12 months.The primary outcome measure is retinal ganglion cell-inner plexiform layer thickness at 12 months after treatment.The secondary outcome measures include retinal ganglion cell-inner plexiform layer thickness before and 1,3,6,and 9 months after treatment,retinal nerve fiber layer thickness,best-corrected visual acuity,macular area thickness,and choroidal thickness before and 1,3,6,9,and 12 months after treatment.Safety measure is the incidence of adverse events at 1,3,6,9,and 12 months after treatment.The study protocol hopes to validate the better efficacy and safety of the combined treatment in patients with diabetic macula compared with the other two monotherapies alone during the 12-month follow-up period.The trial is designed to focus on clarifying the time-effect relationship between imaging measures related to the integrity of retinal ganglion cells and best-corrected visual acuity.The trial protocol was approved by the Medical Ethics Committee of the Affiliated Hospital of Beihua University with approval No.(2023)(26)on April 25,2023,and was registered with the Chinese Clinical Trial Registry(registration number:ChiCTR2300072478,June 14,2023,protocol version:2.0).展开更多
The 3D radar reflectivity produced by a mosaic software system, with measurements from 29 operational weather radars in the Yangtze River–Huaihe River Basins(YRHRB) during the mei-yu season of 2007, is compared to co...The 3D radar reflectivity produced by a mosaic software system, with measurements from 29 operational weather radars in the Yangtze River–Huaihe River Basins(YRHRB) during the mei-yu season of 2007, is compared to coincident TRMM PR observations in order to evaluate the value of the ground-based radar reflectivity mosaic in characterizing the 3D structures of mei-yu precipitation. Results show reasonable agreement in the composite radar reflectivity between the two datasets,with a correlation coefficient of 0.8 and a mean bias of -1 dB. The radar mosaic data at constant altitudes are reasonably consistent with the TRMM PR observations in the height range of 2–5 km, revealing essentially the same spatial distribution of radar echo and nearly identical histograms of reflectivity. However, at altitudes above 5 km, the mosaic data overestimate reflectivity and have slower decreasing rates with height compared to the TRMM PR observations. The areas of convective and stratiform precipitation, based on the mosaic reflectivity distribution at 3-km altitude, are highly correlated with the corresponding regions in the TRMM products, with correlation coefficients of 0.92 and 0.97 and mean relative differences of -7.9% and -2.5%, respectively. Finally, the usefulness of the mosaic reflectivity at 3-km altitude at 6-min intervals is illustrated using a mesoscale convective system that occurred over the YRHRB.展开更多
In recent years, spiking neural networks(SNNs) have received increasing attention of research in the field of artificial intelligence due to their high biological plausibility, low energy consumption, and abundant spa...In recent years, spiking neural networks(SNNs) have received increasing attention of research in the field of artificial intelligence due to their high biological plausibility, low energy consumption, and abundant spatio-temporal information.However, the non-differential spike activity makes SNNs more difficult to train in supervised training. Most existing methods focusing on introducing an approximated derivative to replace it, while they are often based on static surrogate functions. In this paper, we propose a progressive surrogate gradient learning for backpropagation of SNNs, which is able to approximate the step function gradually and to reduce information loss. Furthermore, memristor cross arrays are used for speeding up calculation and reducing system energy consumption for their hardware advantage. The proposed algorithm is evaluated on both static and neuromorphic datasets using fully connected and convolutional network architecture, and the experimental results indicate that our approach has a high performance compared with previous research.展开更多
Ritodrine hydrochloride(ritodrine)is a β2-adrenoceptor stimulant that has been effectively prescribed for the prevention of premature labor.[1]In clinical practice,one of the biggest drawbacks ofβ-mimetics is their ...Ritodrine hydrochloride(ritodrine)is a β2-adrenoceptor stimulant that has been effectively prescribed for the prevention of premature labor.[1]In clinical practice,one of the biggest drawbacks ofβ-mimetics is their adverse eff ects,including arrhythmias,myocardial ischemia,and pulmonary edema,due to theirβ-sympathomimetic effects.[2]Here,we report a rare pregnant case of spontaneous coronary artery dissection(SCAD)after intravenous infusion of ritodrine.展开更多
This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenz...This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid(TNBS),specifically through autophagy modulation.Methods:BM-MSCs were collected from BALB/c mice for subsequent experiments.The study employed cell counting kits(CCK-8)to investigate the impact of the MSC-conditioned medium(M medium)on the proliferation of RAW264.7 macrophages.The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux.The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blot analysis was employed to determine the presence of a binding interaction between NOD-like receptor protein 3(NLRP3)and autophagy.Furthermore,a colitis mouse model was established by TNBS induction.Clinical disease activity score was assessed regularly,and histological and morphometric analyses were performed on colonic tissues.Inflammatory serum cytokines were identified using an enzyme-linked immunosorbent assay.Results:BM-MSCs significantly promoted the proliferation of RAW264.7.In vitro lipopolysaccharide(LPS)-stimulated RAW264.7 cells,treated with BM-MSCs,triggered autophagy and inhibited cytokine mRNA expression.Additionally,in LPS-induced RAW264.7,BM-MSCs enhanced the Beclin1 protein expression and the microtubule-associated protein 1 light chain 3(LC3)-II to LC3-I ratio while suppressing the protein levels of NLRP3 and apoptosis-associated speck-like protein(ASC).Nevertheless,3-methyladenine(3-MA),an inhibitor of autophagy,prevented the impact of BM-MSCs by reducing the levels of NLRP3 and ASC proteins,suggesting that autophagy triggered the inhibition of the NLRP3 inflammasome.In comparison to the mice in the TNBS group,the mice in the TNBS+MSC group displayed a more acute form of colitis,and the IL1βand IL18 cytokines in their serum were lowered as well.In the meantime,3-MA raised IL1βand IL18 cytokine levels and worsened TNBS-induced experimental colitis.Conclusions:BM-MSCs can suppress inflammation in TNBS-induced experimental mice by inhibiting the NLRP3 inflammasome,thereby enhancing autophagy.展开更多
AIM:To investigate the function and mechanism of mi R-133a in gastric cancer(GC)and its relationship with clinicopathological characteristics of GC.METHODS:A total of 105 GC patients who underwent surgical resection a...AIM:To investigate the function and mechanism of mi R-133a in gastric cancer(GC)and its relationship with clinicopathological characteristics of GC.METHODS:A total of 105 GC patients who underwent surgical resection as primary treatment were selected for this study.Real-time quantitative reverse transcriptase polymerase chain(q RT-PCR)was used to examine the expression levels of mi R-133a in human GC and adjacent non-tumor tissues,as well as in GC cell lines(SGC-7901,BGC-823,MGC-803,and AGS)and a human gastric mucosal epithelial cell line(GES-1).The biological role of mi RNA(mi R)-133a was assessed in the GC cell lines using MTT,apoptosis,migration and invasion,and colony formation assays,and xenograft tumorigenesis.q RT-PCR and western blot analyses were used to evaluate the potential target gene expression of mi R-133a.Pearson’s correlation was calculated to evaluate the correlation between mi R-133a and insulinlike growth factor 1 receptor(IGF1R)expression.The regulation of IGF1R by mi R-133a was verified using the luciferase reporter assay.RESULTS:In 80%of the 105 GC patients,the mean expression of mi R-133a was significantly downregulated in tumor tissues compared with adjacent normal tissues(1.215±0.1477 vs 3.093±0.4104,P<0.0001).Downregulation of mi R-133a was significantly correlated with the degree of differentiation(P=0.01),local invasion(P=0.001)and TNM stage(P=0.02)in GC patients.Compared with a control construct,forced expression of mi R-133a in GC cell lines inhibited proliferation(0.4787±0.0219 vs 0.7050±0.0147,P=0.0013 in SGC-7901 cells;and 0.5448±0.0085vs 0.7270±0.0084,P=0.001 in MGC-803 cells);migration(0.6333±0.0233 vs 1.037±0.0584,P=0.003 in SGC-7901 cells;0.6126±0.0311 vs 1.024±0.0456,P=0.0017 in MGC-803 cells);and invasion(0.613±0.0399 vs 1.033±0.0278,P=0.0013 in SGC-7901 cells;0.7433±0.0221 vs 1.017±0.0311,P=0.002 in MGC-803 cells).It also induced apoptosis(18.19%±0.2483%vs 5.887%±0.3837%,P<0.0001 in SGC-7901 cells;22.69%±0.7846%vs9.347%±0.3012%,P<0.0001 in MGC-803 cells).Furthermore,mi R-133a inhibited tumor growth and xenograft tumorigenesis of SGC-7901 cells in vivo.In addition,we identified IGF1R as a regulatory target of mi R-133a in GC.CONCLUSION:This study suggests that mi R-133a is downregulated in GC and functions as a tumor suppressor in vitro and in vivo partly by repressing IGF1R.展开更多
This paper focuses on advanced analysis techniques and design considerations of DC interference generated by HVDC electrodes during normal bipolar and temporary monopolar operations on neighboring metallic utilities, ...This paper focuses on advanced analysis techniques and design considerations of DC interference generated by HVDC electrodes during normal bipolar and temporary monopolar operations on neighboring metallic utilities, with a special emphasis on buried gas and oil pipelines. This study examines the level of pipeline corrosion, the safety status in the vicinity of exposed appurtenances and the impact of DC interference on the integrity of insulating flanges and impressed current cathodic protection (ICCP) systems. Computation results obtained for different soil models show that different soils can lead to completely different DC interference effects. The results and conclusions presented here can be used as a reference to analyze the severity of DC interference on pipelines due to proximate HVDC electrodes.展开更多
The structural phase transitions of bismuth under rapid compression has been investigated in a dynamic diamond anvil cell using time-resolved synchrotron x-ray diffraction. As the pressure increases, the transformatio...The structural phase transitions of bismuth under rapid compression has been investigated in a dynamic diamond anvil cell using time-resolved synchrotron x-ray diffraction. As the pressure increases, the transformations from phase I,to phase II, to phase III, and then to phase V have been observed under different compression rates at 300 K. Compared with static compression results, no new phase transition sequence appears under rapid compression at compression rate from 0.20 GPa/s to 183.8 GPa/s. However, during the process across the transition from phase III to phase V, the volume fraction of product phase as a function of pressure can be well fitted by a compression-rate-dependent sigmoidal curve.The resulting parameters indicate that the activation energy related to this phase transition, as well as the onset transition pressure, shows a compression-rate-dependent performance. A strong dependence of over-pressurization on compression rate occurs under rapid compression. A formula for over-pressure has been proposed, which can be used to quantify the over-pressurization.展开更多
LiCoO_2 is one of the most important cathode materials for high energy density lithium ion batteries. The compressed behavior of LiCoO_2 under high pressure has been investigated using synchrotron radiation x-ray diff...LiCoO_2 is one of the most important cathode materials for high energy density lithium ion batteries. The compressed behavior of LiCoO_2 under high pressure has been investigated using synchrotron radiation x-ray diffraction. It is found that LiCoO_2 maintains hexagonal symmetry up to the maximum pressure of 30.1 GPa without phase transition. The elastic modulus at ambient pressure is 159.5(2.2) GPa and its first derivative is 3.92(0.23). In addition, the high-pressure compression behavior of LiCoO_2 has been studied by first principles calculations. The derived bulk modulus of LiCoO_2 is 141.6 GPa.展开更多
AIM:To assess the biosafety of a poly(acrylamide-cosodium acrylate)hydrogel(PAH)as a 3D-printed intraocular lens(IOL)material.METHODS:The biosafety of PAH was first evaluated in vitro using human lens epithelial cells...AIM:To assess the biosafety of a poly(acrylamide-cosodium acrylate)hydrogel(PAH)as a 3D-printed intraocular lens(IOL)material.METHODS:The biosafety of PAH was first evaluated in vitro using human lens epithelial cells(LECs)and the ARPE19 cell line,and a cell counting kit-8(CCK-8)assay was performed to investigate alterations in cell proliferation.A thin film of PAH and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively,and a sham surgery served as control group.The anterior segment photographs,intraocular pressure(IOP),blood parameters and electroretinograms(ERG)were recorded.Inflammatory cytokines in the aqueous humor,such as TNFαand IL-8,were examined by ELISA.Cell apoptosis of the retina was investigated by TUNEL assay,and macro PAHge activation was detected by immunostaining.RESULTS:PAH did not slow cell proliferation when cocultured with human LECs or ARPE19 cells.The implantation of a thin film of a 3 D-printed IOL composed of PAH did not affect the IOP,blood parameters,ERG or optical structure in any of the three experimental groups(n=3 for each).Both TNFαand IL-8 in the aqueous humor of PAH group were transiently elevated 1 wk post-operation and recovered to normal levels at 1 and 3 mo post-operation.Iba1+macroPAHges in the anterior chamber angle in PAH group were increased markedly compared to those of the control group;however,there was no significant difference compared to those in the IOL group.CONCLUSION:PAH is a safe material for 3D printing of personal IOLs that hold great potential for future clinical applications.展开更多
BACKGROUND 17α-Hydroxylase deficiency(17-OHD)is a rare form of congenital adrenal hyperplasia,characterized by hypertension,hypokalemia,and gonadal dysplasia.However,due to the lack of a comprehensive understanding o...BACKGROUND 17α-Hydroxylase deficiency(17-OHD)is a rare form of congenital adrenal hyperplasia,characterized by hypertension,hypokalemia,and gonadal dysplasia.However,due to the lack of a comprehensive understanding of this disease,it is prone to misdiagnosis and missed diagnosis,and there is no complete cure.CASE SUMMARY We report a female patient with 17-OHD.The patient was admitted to the Department of Neurology of our hospital due to limb weakness.During treatment,it was found that the patient’s condition was difficult to correct except for hypokalemia,and her blood pressure was difficult to control with various antihypertensive drugs.She was then transferred to our department for further treatment.On physical examination,the patient's gonadal development was found to be abnormal,and chromosome analysis demonstrated karyotype 46,XY.Considering the possibility of 17-OHD,the cytochrome P450 family 17 subfamily A member 1(CYP17A1)test was performed to confirm the diagnosis.CONCLUSION The clinical manifestations of 17-OHD are complex.Hormone determination,imaging examination,chromosome determination and CYP17A1 gene test are helpful for early diagnosis.展开更多
基金supported by Science and Technology Research Project of Jilin Provincial Department of Education,No.JJKH20220072KJ(to XL)Science and Technology Development Program of Jilin Province,No.20200201495JC(to YL)。
文摘The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic macular edema are anti-vascular endothelial growth factor drugs and laser photocoagulation.However,although the macular thickness can be normalized with each of these two therapies used alone,the vision does not improve in many patients.This might result from the incomplete recovery of retinal ganglion cell injury.Therefore,a prospective,non-randomized,controlled clinical trial was designed to investigate the effect of anti-vascular endothelial growth factor drugs combined with laser photocoagulation on the integrity of retinal ganglion cells in patients with diabetic macular edema and its relationship with vision recovery.In this trial,150 patients with diabetic macular edema will be equally divided into three groups according to therapeutic methods,followed by treatment with anti-vascular endothelial growth factor drugs,laser photocoagulation therapy,and their combination.All patients will be followed up for 12 months.The primary outcome measure is retinal ganglion cell-inner plexiform layer thickness at 12 months after treatment.The secondary outcome measures include retinal ganglion cell-inner plexiform layer thickness before and 1,3,6,and 9 months after treatment,retinal nerve fiber layer thickness,best-corrected visual acuity,macular area thickness,and choroidal thickness before and 1,3,6,9,and 12 months after treatment.Safety measure is the incidence of adverse events at 1,3,6,9,and 12 months after treatment.The study protocol hopes to validate the better efficacy and safety of the combined treatment in patients with diabetic macula compared with the other two monotherapies alone during the 12-month follow-up period.The trial is designed to focus on clarifying the time-effect relationship between imaging measures related to the integrity of retinal ganglion cells and best-corrected visual acuity.The trial protocol was approved by the Medical Ethics Committee of the Affiliated Hospital of Beihua University with approval No.(2023)(26)on April 25,2023,and was registered with the Chinese Clinical Trial Registry(registration number:ChiCTR2300072478,June 14,2023,protocol version:2.0).
基金supported by the National Basic Research (973) Program (Grant Nos. 2013CB430100 and 2012CB417202)the National Natural Science Foundation of China (Grant Nos. 41175049 and 91437104)the National Key Technology R&D Program (Grant No. 2012BAC22B00) of China
文摘The 3D radar reflectivity produced by a mosaic software system, with measurements from 29 operational weather radars in the Yangtze River–Huaihe River Basins(YRHRB) during the mei-yu season of 2007, is compared to coincident TRMM PR observations in order to evaluate the value of the ground-based radar reflectivity mosaic in characterizing the 3D structures of mei-yu precipitation. Results show reasonable agreement in the composite radar reflectivity between the two datasets,with a correlation coefficient of 0.8 and a mean bias of -1 dB. The radar mosaic data at constant altitudes are reasonably consistent with the TRMM PR observations in the height range of 2–5 km, revealing essentially the same spatial distribution of radar echo and nearly identical histograms of reflectivity. However, at altitudes above 5 km, the mosaic data overestimate reflectivity and have slower decreasing rates with height compared to the TRMM PR observations. The areas of convective and stratiform precipitation, based on the mosaic reflectivity distribution at 3-km altitude, are highly correlated with the corresponding regions in the TRMM products, with correlation coefficients of 0.92 and 0.97 and mean relative differences of -7.9% and -2.5%, respectively. Finally, the usefulness of the mosaic reflectivity at 3-km altitude at 6-min intervals is illustrated using a mesoscale convective system that occurred over the YRHRB.
基金Project supported by the Natural Science Foundation of Chongqing(Grant No.cstc2021jcyj-msxmX0565)the Fundamental Research Funds for the Central Universities(Grant No.SWU021002)the Graduate Research Innovation Project of Chongqing(Grant No.CYS22242)。
文摘In recent years, spiking neural networks(SNNs) have received increasing attention of research in the field of artificial intelligence due to their high biological plausibility, low energy consumption, and abundant spatio-temporal information.However, the non-differential spike activity makes SNNs more difficult to train in supervised training. Most existing methods focusing on introducing an approximated derivative to replace it, while they are often based on static surrogate functions. In this paper, we propose a progressive surrogate gradient learning for backpropagation of SNNs, which is able to approximate the step function gradually and to reduce information loss. Furthermore, memristor cross arrays are used for speeding up calculation and reducing system energy consumption for their hardware advantage. The proposed algorithm is evaluated on both static and neuromorphic datasets using fully connected and convolutional network architecture, and the experimental results indicate that our approach has a high performance compared with previous research.
文摘Ritodrine hydrochloride(ritodrine)is a β2-adrenoceptor stimulant that has been effectively prescribed for the prevention of premature labor.[1]In clinical practice,one of the biggest drawbacks ofβ-mimetics is their adverse eff ects,including arrhythmias,myocardial ischemia,and pulmonary edema,due to theirβ-sympathomimetic effects.[2]Here,we report a rare pregnant case of spontaneous coronary artery dissection(SCAD)after intravenous infusion of ritodrine.
文摘This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid(TNBS),specifically through autophagy modulation.Methods:BM-MSCs were collected from BALB/c mice for subsequent experiments.The study employed cell counting kits(CCK-8)to investigate the impact of the MSC-conditioned medium(M medium)on the proliferation of RAW264.7 macrophages.The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux.The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blot analysis was employed to determine the presence of a binding interaction between NOD-like receptor protein 3(NLRP3)and autophagy.Furthermore,a colitis mouse model was established by TNBS induction.Clinical disease activity score was assessed regularly,and histological and morphometric analyses were performed on colonic tissues.Inflammatory serum cytokines were identified using an enzyme-linked immunosorbent assay.Results:BM-MSCs significantly promoted the proliferation of RAW264.7.In vitro lipopolysaccharide(LPS)-stimulated RAW264.7 cells,treated with BM-MSCs,triggered autophagy and inhibited cytokine mRNA expression.Additionally,in LPS-induced RAW264.7,BM-MSCs enhanced the Beclin1 protein expression and the microtubule-associated protein 1 light chain 3(LC3)-II to LC3-I ratio while suppressing the protein levels of NLRP3 and apoptosis-associated speck-like protein(ASC).Nevertheless,3-methyladenine(3-MA),an inhibitor of autophagy,prevented the impact of BM-MSCs by reducing the levels of NLRP3 and ASC proteins,suggesting that autophagy triggered the inhibition of the NLRP3 inflammasome.In comparison to the mice in the TNBS group,the mice in the TNBS+MSC group displayed a more acute form of colitis,and the IL1βand IL18 cytokines in their serum were lowered as well.In the meantime,3-MA raised IL1βand IL18 cytokine levels and worsened TNBS-induced experimental colitis.Conclusions:BM-MSCs can suppress inflammation in TNBS-induced experimental mice by inhibiting the NLRP3 inflammasome,thereby enhancing autophagy.
基金Supported by Nanjing Medical University institutional grant,No.11ZLKF06(to SYC and LMT)the Changzhou basic research program of science and technology,No.CJ20122014
文摘AIM:To investigate the function and mechanism of mi R-133a in gastric cancer(GC)and its relationship with clinicopathological characteristics of GC.METHODS:A total of 105 GC patients who underwent surgical resection as primary treatment were selected for this study.Real-time quantitative reverse transcriptase polymerase chain(q RT-PCR)was used to examine the expression levels of mi R-133a in human GC and adjacent non-tumor tissues,as well as in GC cell lines(SGC-7901,BGC-823,MGC-803,and AGS)and a human gastric mucosal epithelial cell line(GES-1).The biological role of mi RNA(mi R)-133a was assessed in the GC cell lines using MTT,apoptosis,migration and invasion,and colony formation assays,and xenograft tumorigenesis.q RT-PCR and western blot analyses were used to evaluate the potential target gene expression of mi R-133a.Pearson’s correlation was calculated to evaluate the correlation between mi R-133a and insulinlike growth factor 1 receptor(IGF1R)expression.The regulation of IGF1R by mi R-133a was verified using the luciferase reporter assay.RESULTS:In 80%of the 105 GC patients,the mean expression of mi R-133a was significantly downregulated in tumor tissues compared with adjacent normal tissues(1.215±0.1477 vs 3.093±0.4104,P<0.0001).Downregulation of mi R-133a was significantly correlated with the degree of differentiation(P=0.01),local invasion(P=0.001)and TNM stage(P=0.02)in GC patients.Compared with a control construct,forced expression of mi R-133a in GC cell lines inhibited proliferation(0.4787±0.0219 vs 0.7050±0.0147,P=0.0013 in SGC-7901 cells;and 0.5448±0.0085vs 0.7270±0.0084,P=0.001 in MGC-803 cells);migration(0.6333±0.0233 vs 1.037±0.0584,P=0.003 in SGC-7901 cells;0.6126±0.0311 vs 1.024±0.0456,P=0.0017 in MGC-803 cells);and invasion(0.613±0.0399 vs 1.033±0.0278,P=0.0013 in SGC-7901 cells;0.7433±0.0221 vs 1.017±0.0311,P=0.002 in MGC-803 cells).It also induced apoptosis(18.19%±0.2483%vs 5.887%±0.3837%,P<0.0001 in SGC-7901 cells;22.69%±0.7846%vs9.347%±0.3012%,P<0.0001 in MGC-803 cells).Furthermore,mi R-133a inhibited tumor growth and xenograft tumorigenesis of SGC-7901 cells in vivo.In addition,we identified IGF1R as a regulatory target of mi R-133a in GC.CONCLUSION:This study suggests that mi R-133a is downregulated in GC and functions as a tumor suppressor in vitro and in vivo partly by repressing IGF1R.
文摘This paper focuses on advanced analysis techniques and design considerations of DC interference generated by HVDC electrodes during normal bipolar and temporary monopolar operations on neighboring metallic utilities, with a special emphasis on buried gas and oil pipelines. This study examines the level of pipeline corrosion, the safety status in the vicinity of exposed appurtenances and the impact of DC interference on the integrity of insulating flanges and impressed current cathodic protection (ICCP) systems. Computation results obtained for different soil models show that different soils can lead to completely different DC interference effects. The results and conclusions presented here can be used as a reference to analyze the severity of DC interference on pipelines due to proximate HVDC electrodes.
基金supported by the Joint Fund of the National Natural Science Foundation of China and the China Academy of Engineering Physics(NSAF)(Grant No.U1530134)the Foundation of National Key Laboratory of Shock Wave and Detonation Physics,China(Grant No.6142A0306010817)the Chinese Academy of Sciences(Grant Nos.KJCX2-SW-N20 and KJCX2-SW-N03)
文摘The structural phase transitions of bismuth under rapid compression has been investigated in a dynamic diamond anvil cell using time-resolved synchrotron x-ray diffraction. As the pressure increases, the transformations from phase I,to phase II, to phase III, and then to phase V have been observed under different compression rates at 300 K. Compared with static compression results, no new phase transition sequence appears under rapid compression at compression rate from 0.20 GPa/s to 183.8 GPa/s. However, during the process across the transition from phase III to phase V, the volume fraction of product phase as a function of pressure can be well fitted by a compression-rate-dependent sigmoidal curve.The resulting parameters indicate that the activation energy related to this phase transition, as well as the onset transition pressure, shows a compression-rate-dependent performance. A strong dependence of over-pressurization on compression rate occurs under rapid compression. A formula for over-pressure has been proposed, which can be used to quantify the over-pressurization.
基金Project supported by the Program of Education Department of Sichuan Province of China(Grant No.18ZB0506)the Project of Sichuan University o Arts and Science,China(Grant No.2017KZ001Z)+1 种基金Outstanding Talent Introduction Project of Henan Institute of Science and Technology,China(Gran No.203010617011)performed at 4W2 beamline of Beijing Synchrotron Radiation Facility(BSRF),which was supported by Chinese Academy of Sciences(Grant Nos.KJCX2-SWN03 and KJCX2-SW-N20)
文摘LiCoO_2 is one of the most important cathode materials for high energy density lithium ion batteries. The compressed behavior of LiCoO_2 under high pressure has been investigated using synchrotron radiation x-ray diffraction. It is found that LiCoO_2 maintains hexagonal symmetry up to the maximum pressure of 30.1 GPa without phase transition. The elastic modulus at ambient pressure is 159.5(2.2) GPa and its first derivative is 3.92(0.23). In addition, the high-pressure compression behavior of LiCoO_2 has been studied by first principles calculations. The derived bulk modulus of LiCoO_2 is 141.6 GPa.
基金Supported by the Military Medical Science and Technology Innovation Plan(No.SWH2016LHYS-07)Cultivation Plan of Military Medical Youth Sci-tech(No.18QNP001)。
文摘AIM:To assess the biosafety of a poly(acrylamide-cosodium acrylate)hydrogel(PAH)as a 3D-printed intraocular lens(IOL)material.METHODS:The biosafety of PAH was first evaluated in vitro using human lens epithelial cells(LECs)and the ARPE19 cell line,and a cell counting kit-8(CCK-8)assay was performed to investigate alterations in cell proliferation.A thin film of PAH and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively,and a sham surgery served as control group.The anterior segment photographs,intraocular pressure(IOP),blood parameters and electroretinograms(ERG)were recorded.Inflammatory cytokines in the aqueous humor,such as TNFαand IL-8,were examined by ELISA.Cell apoptosis of the retina was investigated by TUNEL assay,and macro PAHge activation was detected by immunostaining.RESULTS:PAH did not slow cell proliferation when cocultured with human LECs or ARPE19 cells.The implantation of a thin film of a 3 D-printed IOL composed of PAH did not affect the IOP,blood parameters,ERG or optical structure in any of the three experimental groups(n=3 for each).Both TNFαand IL-8 in the aqueous humor of PAH group were transiently elevated 1 wk post-operation and recovered to normal levels at 1 and 3 mo post-operation.Iba1+macroPAHges in the anterior chamber angle in PAH group were increased markedly compared to those of the control group;however,there was no significant difference compared to those in the IOL group.CONCLUSION:PAH is a safe material for 3D printing of personal IOLs that hold great potential for future clinical applications.
文摘BACKGROUND 17α-Hydroxylase deficiency(17-OHD)is a rare form of congenital adrenal hyperplasia,characterized by hypertension,hypokalemia,and gonadal dysplasia.However,due to the lack of a comprehensive understanding of this disease,it is prone to misdiagnosis and missed diagnosis,and there is no complete cure.CASE SUMMARY We report a female patient with 17-OHD.The patient was admitted to the Department of Neurology of our hospital due to limb weakness.During treatment,it was found that the patient’s condition was difficult to correct except for hypokalemia,and her blood pressure was difficult to control with various antihypertensive drugs.She was then transferred to our department for further treatment.On physical examination,the patient's gonadal development was found to be abnormal,and chromosome analysis demonstrated karyotype 46,XY.Considering the possibility of 17-OHD,the cytochrome P450 family 17 subfamily A member 1(CYP17A1)test was performed to confirm the diagnosis.CONCLUSION The clinical manifestations of 17-OHD are complex.Hormone determination,imaging examination,chromosome determination and CYP17A1 gene test are helpful for early diagnosis.
