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Effect of solanine on the membrane potential of mitochondria in HepG_2 cells and [Ca^(2+)]i in the cells 被引量:17
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作者 Shi-Yong Gao Qiu-Juan Wang yu-bin ji 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第21期3359-3367,共9页
AIM: To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca^2+]i in the cells, and to uncover the mechanism by which solanine induces apoptosis.METHODS: HepG2 cells were ... AIM: To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca^2+]i in the cells, and to uncover the mechanism by which solanine induces apoptosis.METHODS: HepG2 cells were double stained with AO/EB, and morphological changes of the cells were observed using laser confocal scanning microscopy (LCSM). HepG2 cells were stained with TMRE, and change in the membrane potential of mitochondria in the cells were observed using LCSM. HepG2 cells were double stained with Fluo-3/AM, and change of [Ca^2+]i in the cells were observed using LCSM. HepG2 cells were double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca^2+]i in the cells were observed using LCSM.RESULTS: Cells in treated groups showed typical signs of apoptosis. Staining with TMRE showed that solanine could lower membrane potential; staining with Fluo-3/AM showed that solanine could increase the concentration of Ca^2+ in tumor cells; and those of double staining with TMRE and Fluo-3/AM showed that solanine could increase the concentration of Ca^2+ in the cells at the same time as it lowered the membrane potential of mitochondria.CONCLUSION: Solanine opens up the PT channels in the membrane by lowering the membrane potential, leading to Ca^2+ being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca^2+ in the cell, turning on the mechanism for apoptosis. 展开更多
关键词 SOLANINE Hepatocarcinomatic cell Ca^2+ in the cell Membrane potential Laser confocal scanning microscopy
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Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells 被引量:2
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作者 yu-bin ji Shi-yong GAO 《浙江中西医结合杂志》 2008年第9期701-706,共6页
Objective: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. Methods: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apopto... Objective: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. Methods: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. Results: NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells. Conclusion: NAT can inhibit apoptosis in HepG2 cells. 展开更多
关键词 HEPG2细胞 细胞凋亡 抑制剂 生物活性
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