BACKGROUND Primary liver cancer is a prevalent and deadly cancer type.Despite treatment advances,prognosis remains poor,with high recurrence rates.Early detection is crucial but challenging due to the disease’s insid...BACKGROUND Primary liver cancer is a prevalent and deadly cancer type.Despite treatment advances,prognosis remains poor,with high recurrence rates.Early detection is crucial but challenging due to the disease’s insidious nature.Myosin proteins play significant roles in cancer development,influencing cell migration,invasion,and tumor suppression.MYL6B,a myosin light chain,is involved in various cellular processes and has been associated with poor prognosis in colorectal adenocarcinoma and potential as a biomarker in breast cancer.AIM To investigate the expression of MYL6B in liver hepatocellular carcinoma(LIHC)and its impact on prognosis and potential mechanisms of action using bioinformatics methods.METHODS The expression of MYL6B in pan-cancer and normal tissues was analyzed using the gene expression profiling interactive analysis 2 and tumor immune estimation resource databases.The expression level of MYL6B in LIHC tissues and its relationship with prognosis were analyzed,immunohistochemical analysis of MYL6B and its effect on immune cell infiltration,and the protein network were further studied.RESULTS MYL6B was highly expressed in diffuse large b-cell lymphoma,LIHC,pancreatic adenocarcinoma,skin cutaneous melanoma,thymoma,uterine corpus endometrial carcinoma,uterine carcinosarcoma,and lowly expressed in kidney chromophobe,acute myeloid leukemia,testicular germ cell tumors.The expression level of MYL6B was significantly different between cancer and normal tissues.It had a significant impact on both overall survival and disease-free survival.MYL6B is highly expressed in hepatocellular carcinoma and its expression level increases with cancer progression.High MYL6B expression is associated with poor prognosis in terms of overall survival and recurrence-free survival.The immunohistochemical level of MYL6B is high in hepatocellular carcinoma tissues,and MYL6B has a high level of immune infiltration inflammation.In protein network analysis,MYL6B is correlated with MYL2,MYL6,MYL9,MYLK4,MYLK2,MYL12A,MYL12B,MYH11,MYH9 and MYH10.CONCLUSION The expression level of MYL6B in LIHC was significantly higher than in normal liver tissues,and it was correlated with the degree of differentiation survival rate,and immune infiltration.MYL6B is a potential target for LIHC treatment.展开更多
Objective There are still a high proportion of patients with ST-segment elevation myocardial infarction （STEMI） missing out early reperfusion even in the primary percutaneous coronary intervention （PCI） era. Most ...Objective There are still a high proportion of patients with ST-segment elevation myocardial infarction （STEMI） missing out early reperfusion even in the primary percutaneous coronary intervention （PCI） era. Most of them are stable latecomers, but the optimal time to undergo delayed PCI for stable ones remains controversial. Methods We investigated all STEMI patients who underwent delayed PCI （2-28 days after STEMI） during 2007-2010 in Beijing and excluded patients with hemodynamic instability. The primary outcome was maj or adverse cardiovascular events （MACEs）. Results This study finally enrolled 5,417 STEMI patients and assigned them into three groups according to individual delayed time （Early group, 55.9%; Medium group, 35.4%; Late group, 8.7%）. During 1-year follow-up, MACEs occurred in 319 patients. The incidence of MACEs were respectively 7.1%, 5.6% and 6.7% among three groups. The Medium group had less recurrent myocardial infarction plus cardiac death （hazard ratio, 0.525; 95% confidence interval, 0.294-0.938, P = 0.030） than Late group and less repeat revascularization （hazard ratio, 0.640; 95% confidence interval, 0.463-0.883, P = 0.007） than Early group in pairwise comparisons. We depicted the incidence of major adverse cardiovascular event （MACE） by delayed time as a quadratic curve and found the bottom appeared at day 14. Conclusions The delayed PCI time varied in the real-world practice, but undergoing operations on the second week after STEMI had greater survival benefit and less adverse events for whom without early reperfusion and hemodynamic instability.展开更多
Chlorogenic acid（5-caffeoylquinic acid, CGA） is a phenolic compound that is found ubiquitously in plants, fruits and vegetables and is formed via the esterification of caffeic acid and quinic acid. In addition to it...Chlorogenic acid（5-caffeoylquinic acid, CGA） is a phenolic compound that is found ubiquitously in plants, fruits and vegetables and is formed via the esterification of caffeic acid and quinic acid. In addition to its notable biological functions against cardiovascular diseases, type-2 diabetes and inflammatory conditions, CGA was recently hypothesized to be an alternative for the treatment of neurological diseases such as Alzheimer＇s disease and neuropathic pain disorders. However, its mechanism of action is unclear.Voltage-gated potassium channel（Kv） is a crucial factor in the electro-physiological processes of sensory neurons. Kv has also been identified as a potential therapeutic target for inflammation and neuropathic pain disorders. In this study, we analysed the effects of CGA on the two main subtypes of Kv in trigeminal ganglion neurons, namely, the IK,Aand IK,Vchannels. Trigeminal ganglion（TRG）neurons were acutely disassociated from the rat TRG, and two different doses of CGA（0.2 and 1 mmol·L21） were applied to the cells.Whole-cell patch-clamp recordings were performed to observe alterations in the activation and inactivation properties of the IK,Aand IK,Vchannels. The results demonstrated that 0.2 mmol·L21CGA decreased the peak current density of IK,A. Both 0.2 mmol·L21and1 mmol·L21CGA also caused a significant reduction in the activation and inactivation thresholds of IK,Aand IK,V. CGA exhibited a strong effect on the activation and inactivation velocities of IK,Aand IK,V. These findings provide novel evidence explaining the biological effects of CGA, especially regarding its neurological effects.展开更多
Purpose： This prospective observational study aims to evaluate the accuracy of dead-space fraction derived from the ventilator volumetric capnography （volumetric CO2） or a prediction equation to predict the surviva...Purpose： This prospective observational study aims to evaluate the accuracy of dead-space fraction derived from the ventilator volumetric capnography （volumetric CO2） or a prediction equation to predict the survival of mechanically ventilated patients with acute respiratory distress syndrome （ARDS）. Methods： Consecutive VDJVT measurements were obtained based upon a prediction equation validated by Frankenfield et al for dead-space ventilation fraction： VD/VT = 0.320 ＋ 0.0106 （PaCO2-ETCO2） 4- 0.003 （RR） ＋ 0.0015 （age） in adult patients who had infection-related severe pneumonia and were confirmed as having ARDS. Here PaCO2 is the arterial partial pressure of carbon dioxide in mmHg; ETC02, the end- tidal carbon dioxide measurement in mmHg; RR, respiratory rate per minute; and age in years. Once the patient had intubation, positive end expiratory pressure was adjusted and after Phigh reached a steady state, VD/VT was measured and recorded as the data for the first day. VD/VT measurement was repeated on days 2, 3, 4, 5 and 6. Meanwhile we collected dead-space fraction directly from the ventilator volu- metric CO2 and recorded it as Vd/Vt. We analyzed the changes in VD/VT and Vd/Vt over the f-day period to determine their accuracy in predicting the survival of ARDS patients. Results： Overall, 46 patients with ARD5 met the inclusion criteria and 24 of them died, During the first 6 days of intubation, VD/VT was significantly higher in nonsurvivors on day 4 （0.70 ± 0,01 vs 0.57 ± 0.01 ）, day 5 （0.73 ± 0.01 vs, 0.54 ± 0.01 ）, and day 6 （0.73 ±0,02 vs. 0.54 ± 0.01 ） （all p - 0.000）. Vd/Vt showed no significant difference on days 1-4 but it was much higher in nonsurvivors on day 5 （0.45 ± 0.04 vs. 0.41 ±0.06） and day 6 （0.47 ± 0,05 vs. 0.40 ± 0.03） （both p - 0.008）. VD/VT on the fourth day was more accurate to predict survival than Vd/Vt. The area under the receiver-operating characteristic curve for VD/VT and Vd/Vt in evaluating ARDS patients survival was day 4 （0,974 ± 0.093 vs. 0.701 ± 0.023, p = 0.0024） with the 95% confidence interval being 0.857-0.999 vs, 0.525-0.84L Conclusion： Compared with Vd/Vt derived from ventilator volumetric CO2, VD/VT on day 4 calculated by Frankenfield et al＇s equation can more accurately predict the survival of ARDS patients.展开更多
Lithium sulfur battery(LSB) is widely considered as a next-generation battery system due to its high theoretical energy density of 2567 Wh kg-1.However,several inherent issues obstruct the business application of LSB....Lithium sulfur battery(LSB) is widely considered as a next-generation battery system due to its high theoretical energy density of 2567 Wh kg-1.However,several inherent issues obstruct the business application of LSB.To address these issues,we assemble core-shell structure Fe_(3)O_(4)@C nanodots with 5 nm diameter as a valid sulfur host via a convenient organic pyrolysis treatment followed by calcination.The nanosized Fe3O4 particles could expose more chemisorption sites and inhibit the polysulfides shuttle.Moreover,the carbon layer could not only increase the conductivity but also ensure structural integrity during cyclic process.Furthermore,the mesoporous channels around Fe_(3)O_(4)@C could supply sufficient space to load sulfur and physically restrict the shuttle of polysulfides.Thus,the resultant S/Fe_(3)O_(4)@C cathode shows a highly initial capacity of 1089 mAh g^(-1) at 0.2 C,even retains 655 mAh g^(-1) over 200 cycles at 1 C.展开更多
To the Editor:There is limited evidence as to the association between outpatient visits in dermatology and meteorological factors.A previous study has focused on the effects of meteorological factors on a certain dise...To the Editor:There is limited evidence as to the association between outpatient visits in dermatology and meteorological factors.A previous study has focused on the effects of meteorological factors on a certain disease,and seemed to lack systematic analysis for a class of diseases.[1]Here,we investigated the short-term effects of meteorological factors on the number of daily outpatient visits for skin diseases.展开更多
文摘BACKGROUND Primary liver cancer is a prevalent and deadly cancer type.Despite treatment advances,prognosis remains poor,with high recurrence rates.Early detection is crucial but challenging due to the disease’s insidious nature.Myosin proteins play significant roles in cancer development,influencing cell migration,invasion,and tumor suppression.MYL6B,a myosin light chain,is involved in various cellular processes and has been associated with poor prognosis in colorectal adenocarcinoma and potential as a biomarker in breast cancer.AIM To investigate the expression of MYL6B in liver hepatocellular carcinoma(LIHC)and its impact on prognosis and potential mechanisms of action using bioinformatics methods.METHODS The expression of MYL6B in pan-cancer and normal tissues was analyzed using the gene expression profiling interactive analysis 2 and tumor immune estimation resource databases.The expression level of MYL6B in LIHC tissues and its relationship with prognosis were analyzed,immunohistochemical analysis of MYL6B and its effect on immune cell infiltration,and the protein network were further studied.RESULTS MYL6B was highly expressed in diffuse large b-cell lymphoma,LIHC,pancreatic adenocarcinoma,skin cutaneous melanoma,thymoma,uterine corpus endometrial carcinoma,uterine carcinosarcoma,and lowly expressed in kidney chromophobe,acute myeloid leukemia,testicular germ cell tumors.The expression level of MYL6B was significantly different between cancer and normal tissues.It had a significant impact on both overall survival and disease-free survival.MYL6B is highly expressed in hepatocellular carcinoma and its expression level increases with cancer progression.High MYL6B expression is associated with poor prognosis in terms of overall survival and recurrence-free survival.The immunohistochemical level of MYL6B is high in hepatocellular carcinoma tissues,and MYL6B has a high level of immune infiltration inflammation.In protein network analysis,MYL6B is correlated with MYL2,MYL6,MYL9,MYLK4,MYLK2,MYL12A,MYL12B,MYH11,MYH9 and MYH10.CONCLUSION The expression level of MYL6B in LIHC was significantly higher than in normal liver tissues,and it was correlated with the degree of differentiation survival rate,and immune infiltration.MYL6B is a potential target for LIHC treatment.
文摘Objective There are still a high proportion of patients with ST-segment elevation myocardial infarction （STEMI） missing out early reperfusion even in the primary percutaneous coronary intervention （PCI） era. Most of them are stable latecomers, but the optimal time to undergo delayed PCI for stable ones remains controversial. Methods We investigated all STEMI patients who underwent delayed PCI （2-28 days after STEMI） during 2007-2010 in Beijing and excluded patients with hemodynamic instability. The primary outcome was maj or adverse cardiovascular events （MACEs）. Results This study finally enrolled 5,417 STEMI patients and assigned them into three groups according to individual delayed time （Early group, 55.9%; Medium group, 35.4%; Late group, 8.7%）. During 1-year follow-up, MACEs occurred in 319 patients. The incidence of MACEs were respectively 7.1%, 5.6% and 6.7% among three groups. The Medium group had less recurrent myocardial infarction plus cardiac death （hazard ratio, 0.525; 95% confidence interval, 0.294-0.938, P = 0.030） than Late group and less repeat revascularization （hazard ratio, 0.640; 95% confidence interval, 0.463-0.883, P = 0.007） than Early group in pairwise comparisons. We depicted the incidence of major adverse cardiovascular event （MACE） by delayed time as a quadratic curve and found the bottom appeared at day 14. Conclusions The delayed PCI time varied in the real-world practice, but undergoing operations on the second week after STEMI had greater survival benefit and less adverse events for whom without early reperfusion and hemodynamic instability.
基金supported by the National Science Foundation of China (Grant No. 81000456)the Science and Technology Department of Sichuan Province (Grant No. 2009SZ0171)
文摘Chlorogenic acid（5-caffeoylquinic acid, CGA） is a phenolic compound that is found ubiquitously in plants, fruits and vegetables and is formed via the esterification of caffeic acid and quinic acid. In addition to its notable biological functions against cardiovascular diseases, type-2 diabetes and inflammatory conditions, CGA was recently hypothesized to be an alternative for the treatment of neurological diseases such as Alzheimer＇s disease and neuropathic pain disorders. However, its mechanism of action is unclear.Voltage-gated potassium channel（Kv） is a crucial factor in the electro-physiological processes of sensory neurons. Kv has also been identified as a potential therapeutic target for inflammation and neuropathic pain disorders. In this study, we analysed the effects of CGA on the two main subtypes of Kv in trigeminal ganglion neurons, namely, the IK,Aand IK,Vchannels. Trigeminal ganglion（TRG）neurons were acutely disassociated from the rat TRG, and two different doses of CGA（0.2 and 1 mmol·L21） were applied to the cells.Whole-cell patch-clamp recordings were performed to observe alterations in the activation and inactivation properties of the IK,Aand IK,Vchannels. The results demonstrated that 0.2 mmol·L21CGA decreased the peak current density of IK,A. Both 0.2 mmol·L21and1 mmol·L21CGA also caused a significant reduction in the activation and inactivation thresholds of IK,Aand IK,V. CGA exhibited a strong effect on the activation and inactivation velocities of IK,Aand IK,V. These findings provide novel evidence explaining the biological effects of CGA, especially regarding its neurological effects.
文摘Purpose： This prospective observational study aims to evaluate the accuracy of dead-space fraction derived from the ventilator volumetric capnography （volumetric CO2） or a prediction equation to predict the survival of mechanically ventilated patients with acute respiratory distress syndrome （ARDS）. Methods： Consecutive VDJVT measurements were obtained based upon a prediction equation validated by Frankenfield et al for dead-space ventilation fraction： VD/VT = 0.320 ＋ 0.0106 （PaCO2-ETCO2） 4- 0.003 （RR） ＋ 0.0015 （age） in adult patients who had infection-related severe pneumonia and were confirmed as having ARDS. Here PaCO2 is the arterial partial pressure of carbon dioxide in mmHg; ETC02, the end- tidal carbon dioxide measurement in mmHg; RR, respiratory rate per minute; and age in years. Once the patient had intubation, positive end expiratory pressure was adjusted and after Phigh reached a steady state, VD/VT was measured and recorded as the data for the first day. VD/VT measurement was repeated on days 2, 3, 4, 5 and 6. Meanwhile we collected dead-space fraction directly from the ventilator volu- metric CO2 and recorded it as Vd/Vt. We analyzed the changes in VD/VT and Vd/Vt over the f-day period to determine their accuracy in predicting the survival of ARDS patients. Results： Overall, 46 patients with ARD5 met the inclusion criteria and 24 of them died, During the first 6 days of intubation, VD/VT was significantly higher in nonsurvivors on day 4 （0.70 ± 0,01 vs 0.57 ± 0.01 ）, day 5 （0.73 ± 0.01 vs, 0.54 ± 0.01 ）, and day 6 （0.73 ±0,02 vs. 0.54 ± 0.01 ） （all p - 0.000）. Vd/Vt showed no significant difference on days 1-4 but it was much higher in nonsurvivors on day 5 （0.45 ± 0.04 vs. 0.41 ±0.06） and day 6 （0.47 ± 0,05 vs. 0.40 ± 0.03） （both p - 0.008）. VD/VT on the fourth day was more accurate to predict survival than Vd/Vt. The area under the receiver-operating characteristic curve for VD/VT and Vd/Vt in evaluating ARDS patients survival was day 4 （0,974 ± 0.093 vs. 0.701 ± 0.023, p = 0.0024） with the 95% confidence interval being 0.857-0.999 vs, 0.525-0.84L Conclusion： Compared with Vd/Vt derived from ventilator volumetric CO2, VD/VT on day 4 calculated by Frankenfield et al＇s equation can more accurately predict the survival of ARDS patients.
基金financially supported by the National Science Foundation of China(Nos.51772296,21878195,21805018 and 21805198)the Foundation for State Key Laboratory of Biochemical Engineering。
文摘Lithium sulfur battery(LSB) is widely considered as a next-generation battery system due to its high theoretical energy density of 2567 Wh kg-1.However,several inherent issues obstruct the business application of LSB.To address these issues,we assemble core-shell structure Fe_(3)O_(4)@C nanodots with 5 nm diameter as a valid sulfur host via a convenient organic pyrolysis treatment followed by calcination.The nanosized Fe3O4 particles could expose more chemisorption sites and inhibit the polysulfides shuttle.Moreover,the carbon layer could not only increase the conductivity but also ensure structural integrity during cyclic process.Furthermore,the mesoporous channels around Fe_(3)O_(4)@C could supply sufficient space to load sulfur and physically restrict the shuttle of polysulfides.Thus,the resultant S/Fe_(3)O_(4)@C cathode shows a highly initial capacity of 1089 mAh g^(-1) at 0.2 C,even retains 655 mAh g^(-1) over 200 cycles at 1 C.
基金supported by grants from the National Natural Science Foundation of China(Nos.81673069,81972920).
文摘To the Editor:There is limited evidence as to the association between outpatient visits in dermatology and meteorological factors.A previous study has focused on the effects of meteorological factors on a certain disease,and seemed to lack systematic analysis for a class of diseases.[1]Here,we investigated the short-term effects of meteorological factors on the number of daily outpatient visits for skin diseases.
