Objective:To observe dynamic changes of levels of monocyte chemotactic protein-1(MCP-1),tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in patients with acute pancreatitis and to investigate its evaluation va...Objective:To observe dynamic changes of levels of monocyte chemotactic protein-1(MCP-1),tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in patients with acute pancreatitis and to investigate its evaluation value on the severity of acute pancreatitis.Methods:A total of 109 patients with acute pancreatitis admitted were divided into mild acute pancreatitis group(MAP group,42 cases),moderately severe acute pancreatitis(MSAP group,35 cases)and severe acute pancreatitis(SAP group,32 cases).ELISA was used to detect the serum levels of MCP-1,TNF-α and IL-8 of patients at day 1,day 4 and day 7 of admission to hospital.Results:The serum levels of MCP-1,TNF-α and IL-8 from MAP group,MSAP group and SAP group at day 1 of admission to hospital all significantly increased.There was a significant difference between MAP group and control group,MSAP group and MAP group,SAP group and MSAP group(P<0.05).The serum concentrations of IL-8 from MASP group and SAP group obviously increased at day 1,and there was significant difference between MASP group and MAP group,SAP group and MSAP group(P<0.05),while the difference between MAP group and control group was not obvious(P>0.05);The serum concentrations of MCP-1,TNF-α and IL-8 from MAP group all reached the highest level at day 4,which were significantly higher than the detection levels at day 1.In MSAP group and SAP group,the serum concentrations of MCP-1,TNF-α and IL-8 were the highest at day 1,which were significantly higher than the detection levels at day 4 and 7.At each detecting timing,the serum concentrations of MCP-1,TNF-α and IL-8 from MSAP group and SAP group were all higher than those of MAP group and MSAP group,respectively.Conclusions:The dynamic changes of serum levels of MCP-1,TNF-α and IL-8 in patients with acute pancreatitis have their rules,and the change rule of MAP group was different with that of MSAP and SAP group,which showed the reference value for the diagnosis and illness severity evaluation of acute pancreatitis.展开更多
Objective: To study the influence of cisplatin implants on transplantation tumor growth and the expression of tissue kallikrein-7(KLK7) and E-cadherin(E-cad) in tumor-bearing mice with gastric cancer. Methods: BALB/c ...Objective: To study the influence of cisplatin implants on transplantation tumor growth and the expression of tissue kallikrein-7(KLK7) and E-cadherin(E-cad) in tumor-bearing mice with gastric cancer. Methods: BALB/c nude mice were collected as experimental animal and were randomly divided into model control group(Group A), tail intravenous injection of cisplatin group(Group B), intratumor injection of cisplatin group(Group C) and cisplatin implants treatment group(Group D). After the drugs intervening, the weight and volume of transplantation tumors were measured on Day 20, Day 30 and Day 40 and serum and KLK7 and E-cad contents in transplanted tumor tissue were examined. Results: On Day 20, Day 30 and Day 40 after treatment, the weight and volume of transplantation tumors of tumorbearing mice in four groups were different(Group A > Group B > Group C > Group D). The contents of KLK-7 and E-cad in tumor tissue and serum of tumor-bearing mice in four groups were different(Group A > Group B > Group C > Group D in KLK-7) and(Group A < Group B < Group C < Group D in E-cad). The weight and volume, and KLK7 and E-cad contents of transplantation tumors in four groups were significant difference(P<0.05). Conclusion: Cisplatin implants can inhibit the growth of transplanted tumor tissue and down-regulated KLK7 expression and up-regulated E-cad expression of tumor-bearing mice with gastric展开更多
Objective:To explore the clinical value of dynamic ultrasound monitoring of optic nerve sheath diameter(ONSD)in the treatment of patients with moderate and severe head injury(CI).Methods:A total of 160 patients with m...Objective:To explore the clinical value of dynamic ultrasound monitoring of optic nerve sheath diameter(ONSD)in the treatment of patients with moderate and severe head injury(CI).Methods:A total of 160 patients with moderate and severe CI admitted to the First Affiliated Hospital of Hainan Medical University from January 2018 to January 2020 were selected and divided into observation group(80 cases)and control group(80 cases)a Januaryccording to the random number table.Patients in control group and observation group were dehydrated to reduce intracranial pressure(ICP)according to clinical symptoms/brain CT and ONSD monitoring guidance.National Institutes of Health Stroke Scale(NIHSS),Acute Physiology and Chronic Health EvaluationⅡ(APACHEⅡ),Glasgow Coma Scale(GCS),complications,prognosis,ICU stay time and mechanical ventilation time were compared between the two groups.Results:NIHSS score[control group:(19.58±3.19)points vs(37.98±5.75)points,observation group:(10.33±2.42)points vs(38.05±5.83)points]and APACHE II score[control group:(14.55±2.17)points vs(19.87±3.50)points,observation group:(8.71±2.03)points vs(20.12±3.56)points]of the two groups at 1 month after injury were significantly lower than those at admission(P<0.05),GCS score[control group:(10.78±1.66)points vs(8.03±1.34)points,observation group:(13.10±1.72)points vs(7.99±1.32)points]were significantly higher than that at admission(P<0.05).At 1 month after injury,NIHSS score[(10.33±2.42)points vs(19.58±3.19)points],APACHE II score[(8.71±2.03)points vs(14.55±2.17)points]in the observation group were significantly lower than those in the control group(P<0.05),and GCS score[(13.10±1.72)points vs(10.78±1.66)points]in the observation group was significantly higher than that in the control group(P<0.05).The proportion of hydrocephalus(2.50%vs 12.50%),total complication rate(5.00%vs 21.25%),proportion of severe disability(5.00%vs 17.50%),proportion of survival in plant man(3.75%vs 15.00%),mortality rate(2.50%vs 12.50%),ICU stay time[(5.01±1.25)d vs(8.38±2.29)D],mechanical ventilation time[(2.18±0.75)D]in observation group were lower than those in the control group,and the good rate(56.25%vs 32.50%)and the total effective rate(93.75%vs 72.50%)in the control group were significantly higher than those in the control group(P<0.05).Conclusion:Dynamic ultrasound monitoring ONSD is effective in guiding dehydration treatment of patients with moderate and severe CI,it can significantly reduce ICP and complications,improve prognosis,which is worthy of promotion and application.展开更多
Objective:To observe dynamic changes of MIP-1α, MIP-1β and MCP-1 in Acute pancreatitis patients, and to evaluate the value of MIP-1α, MIP-1β and MCP-1 in acute pancreatitis severity assessment.Methods: A total of ...Objective:To observe dynamic changes of MIP-1α, MIP-1β and MCP-1 in Acute pancreatitis patients, and to evaluate the value of MIP-1α, MIP-1β and MCP-1 in acute pancreatitis severity assessment.Methods: A total of 112 cases of acute pancreatitis were divided into mild pancreatitis (MAP group, 44 cases), moderate severe acute pancreatitis group (MSAP group, 36 cases) and severe acute pancreatitis group (SAP group, 32 cases). Serum MIP-1α, MIP-1βand MCP-1 levels were detected by enzyme-linked immunosorbent assay in patients at 1st, 4th day, 7th days.Results:(1) In 1st day, Serum concentrations of MIP-1α, MIP-1β and MCP-1 were significantly increased in three Groups, There were significant differences between the control group, MAP, MSAP and SAP group. (2) Serum concentrations of MIP-1α, MIP-1β and MCP-1 reached the peak level on 4th day in MAP group and were significantly higher than the level of those on 1st day. In the MSAP and SAP group, serum concentration of MIP-1α, MIP-1β and MCP-1 reached the peak level on 1st day, and significantly higher than the level of those on the 4th day and 7th day. There was a significant difference between MSAP group and MAP group, SAP group and MSAP group in the serum concentration of MIP-1α, MIP-1β and MCP-1 at each monitoring time.Conclusions:MIP-1α, MIP-1β and MCP-1 levels have the rule of dynamic change in Patients with acute pancreatitis, which are useful in evaluating the severity of the patients with acute pancreatitis.展开更多
The Zr62.55Cu17.55Ni9.9Al10 bulk metallic glass （BMG） was prepared by using copper-mold suction-casting. X-ray diffraction and differential scanning calorimetry were utilized to determine its structure and thermal s...The Zr62.55Cu17.55Ni9.9Al10 bulk metallic glass （BMG） was prepared by using copper-mold suction-casting. X-ray diffraction and differential scanning calorimetry were utilized to determine its structure and thermal stability. Uniaxial compression and Rockwell indenta- tion tests were adopted to study the plastic deformation behavior at room temperature. The results show that the glass transition temperature and the onset temperature of exothermic reaction of the BMG are 651.5 and 748 K, respectively. During the compression test, the BMGs undergo an engineering strain of about 2.5%, i.e., true strain of 2.8%, and then fracture. The BMGs deform via the formation and propagation of shear bands. Under indentation loading, the BMGs deform through the formation of radiation-like and circular shear bands. The circular shear bands form earlier than the radiation-like ones, The formation mechanism of shear bands in the BMGs was analyzed and discussed.展开更多
Objective: To explore the effect of clinically available crystalloids on lactate clearance in patients with septic shock Methods: Sixty-eight patients with septic shock who were treated in our hospital from January 20...Objective: To explore the effect of clinically available crystalloids on lactate clearance in patients with septic shock Methods: Sixty-eight patients with septic shock who were treated in our hospital from January 2017 to January 2019 were enrolled in the study. The selected patients were randomly divided into three groups: sodium lactate Ringer's solution group (LR group), saline group (NS group) and compound sodium chloride injection group (RS group), each group received the corresponding crystal liquid for fluid resuscitation, compare three groups Heart rate (HR), mean arterial pressure (MAP), cardiac output, procalcitonin (PCT), and C-reactive protein (CRP) before and after treatment, compared with the treatment of 24 hours of lactate clearance and APACHE II scores, and the overall hospitalization time and mortality rate of the three groups of patients were statistically compared;Results: (1) The difference of HR, MAP and cardiac output between the three groups before treatment was not statistically significant (P>0.05), treatment for 24h The HR of the post-Lactate Ringer's solution group was lower than that of the control group, and the MAP and cardiac output were higher than the control group (P<0.05). (2) The difference of PCT, CRP and BNP levels between the three groups before and after treatment was not statistically significant. (P>0.05);(3) Compared with the results, there was no statistically significant difference in the clearance rate of lactate clearance between the three groups (P>0.05). Three groups before and after treatment APACHE II score was not statistically significant difference comparison (P> 0.05). (4) Three groups The overall hospitalization time and mortality rate of the patients were not statistically significant (P>0.05). Conclusion: Lactate Ringer's solution can better maintain the circulation capacity and blood pressure, which can alleviate the clinical symptoms of patients with septic shock, but no obvious effect on lactic acid clearance. The effect of resuscitation with clinically available crystalloid sodium lactate Ringer's solution, saline and compound sodium chloride injection was not statistically significant in patients with septic shock.展开更多
Interleukin (IL) 33 is a key cytokine in type II immune and airway diseases. It is abundantly expressed in lung epithelial cells and plays an important role in both innate and adaptive immunity. In innate immunity, IL...Interleukin (IL) 33 is a key cytokine in type II immune and airway diseases. It is abundantly expressed in lung epithelial cells and plays an important role in both innate and adaptive immunity. In innate immunity, IL-33 responds promptly to produce an immune response that maintains homeostasis. In adaptive immunity, IL-33 interacts with various immune cells. At the same time, IL-33 also plays an important role in chronic inflammation of the airway and its remodeling. This article reviews the relevant biological knowledge of IL-33 and its research progress in lung immunity, and discusses the related issues of IL-33 as a lung immune test site and therapeutic target.展开更多
Objective:To investigate the clinical effects of continuous plasma filtration with adsorption and continuous hematodialysis on improving the immune level of patients with sepsis in ICU. Methods:A total of 80 patients ...Objective:To investigate the clinical effects of continuous plasma filtration with adsorption and continuous hematodialysis on improving the immune level of patients with sepsis in ICU. Methods:A total of 80 patients with sepsis were randomly divided into study group and control group, 40 patients in each group. All the patients were treated by routine treatment. Patients in study group were treated by continuous plasma filtration with adsorption and patients in control group were treated by continuous hematodialysis. The blood samples were collected before and after treatment. The immune cell level, inflammatory factors level, APACHEⅡ score and death rate were compared between two groups.Results:All immune cell indexes were significantly improved in both groups. All immune cell indexes in study group were significantly better than those in control group, after treatment. All inflammatory factors were significantly decreased in both groups. All inflammatory factors in study group were significantly better than those in control group, after treatment. The APACHEⅡ in study group was significantly better than that in control group, after treatment. The death rate in study group was significantly less than that in control group.Conclusion:Continuous plasma filtration with adsorption has a great clinical efficacy on patients with sepsis in ICU, which can improve the immune level and prognosis, makes it worth for clinical application.展开更多
Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: ...Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: A total of 50 patients with gastric cancer, and 50 healthy persons were selected as the experimental group, benign gastric disease group and healthy group, respectively. A total of 24 patients in experimental group met surgery indications, and agreed to perform gastrectomy. They were divided into preoperative group and postoperative group. The serums levels of KLK7 and E-cad were detected by enzyme-linked immunosorbent assay.Results: The serum levels of KLK7 and E-cad were significantly higher than that of benign gastric disease group and normal control group. Compared with the experimental group after operation, MMP-2 and MMP-9 were decreased, KLK7 was significantly decreased and E-cad increased significantly after operation. Serum levels of KLK7 and E-cad in experimental group stage I were increased and stage IV decreased. Microvascular invasion was significantly correlated with serum KLK7 and E-cad. There was no significant correlation between tumor size, differentiation degree, depth and expression of KLK7 and E-cad. Serum KLK7 were significantly negatively correlated with E-cad expression in gastric cancer patients. Conclusions:KLK7 and E-cad are involved in the development and metastasis of gastric cancer. The serum expression of KLK7 and E-cad are significantly negatively correlated in patients with stomach cancer. There may be an antagonistic effect in gastric cancer development and metastasis. Serum level of KLK7 and E-cad can be used as indexes for progress monitoring.展开更多
基金supported by Health and Family Planning Commission of Hainan Province,China,Scientific Research Project(Grant No.14A210207)
文摘Objective:To observe dynamic changes of levels of monocyte chemotactic protein-1(MCP-1),tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in patients with acute pancreatitis and to investigate its evaluation value on the severity of acute pancreatitis.Methods:A total of 109 patients with acute pancreatitis admitted were divided into mild acute pancreatitis group(MAP group,42 cases),moderately severe acute pancreatitis(MSAP group,35 cases)and severe acute pancreatitis(SAP group,32 cases).ELISA was used to detect the serum levels of MCP-1,TNF-α and IL-8 of patients at day 1,day 4 and day 7 of admission to hospital.Results:The serum levels of MCP-1,TNF-α and IL-8 from MAP group,MSAP group and SAP group at day 1 of admission to hospital all significantly increased.There was a significant difference between MAP group and control group,MSAP group and MAP group,SAP group and MSAP group(P<0.05).The serum concentrations of IL-8 from MASP group and SAP group obviously increased at day 1,and there was significant difference between MASP group and MAP group,SAP group and MSAP group(P<0.05),while the difference between MAP group and control group was not obvious(P>0.05);The serum concentrations of MCP-1,TNF-α and IL-8 from MAP group all reached the highest level at day 4,which were significantly higher than the detection levels at day 1.In MSAP group and SAP group,the serum concentrations of MCP-1,TNF-α and IL-8 were the highest at day 1,which were significantly higher than the detection levels at day 4 and 7.At each detecting timing,the serum concentrations of MCP-1,TNF-α and IL-8 from MSAP group and SAP group were all higher than those of MAP group and MSAP group,respectively.Conclusions:The dynamic changes of serum levels of MCP-1,TNF-α and IL-8 in patients with acute pancreatitis have their rules,and the change rule of MAP group was different with that of MSAP and SAP group,which showed the reference value for the diagnosis and illness severity evaluation of acute pancreatitis.
基金supported by Health Department of Hainan Province(Qiong 2013-self-financing 08)
文摘Objective: To study the influence of cisplatin implants on transplantation tumor growth and the expression of tissue kallikrein-7(KLK7) and E-cadherin(E-cad) in tumor-bearing mice with gastric cancer. Methods: BALB/c nude mice were collected as experimental animal and were randomly divided into model control group(Group A), tail intravenous injection of cisplatin group(Group B), intratumor injection of cisplatin group(Group C) and cisplatin implants treatment group(Group D). After the drugs intervening, the weight and volume of transplantation tumors were measured on Day 20, Day 30 and Day 40 and serum and KLK7 and E-cad contents in transplanted tumor tissue were examined. Results: On Day 20, Day 30 and Day 40 after treatment, the weight and volume of transplantation tumors of tumorbearing mice in four groups were different(Group A > Group B > Group C > Group D). The contents of KLK-7 and E-cad in tumor tissue and serum of tumor-bearing mice in four groups were different(Group A > Group B > Group C > Group D in KLK-7) and(Group A < Group B < Group C < Group D in E-cad). The weight and volume, and KLK7 and E-cad contents of transplantation tumors in four groups were significant difference(P<0.05). Conclusion: Cisplatin implants can inhibit the growth of transplanted tumor tissue and down-regulated KLK7 expression and up-regulated E-cad expression of tumor-bearing mice with gastric
基金Youth cultivation fund of the First affiliated hospital of Hainan medical university(No.HYFYPY201822)。
文摘Objective:To explore the clinical value of dynamic ultrasound monitoring of optic nerve sheath diameter(ONSD)in the treatment of patients with moderate and severe head injury(CI).Methods:A total of 160 patients with moderate and severe CI admitted to the First Affiliated Hospital of Hainan Medical University from January 2018 to January 2020 were selected and divided into observation group(80 cases)and control group(80 cases)a Januaryccording to the random number table.Patients in control group and observation group were dehydrated to reduce intracranial pressure(ICP)according to clinical symptoms/brain CT and ONSD monitoring guidance.National Institutes of Health Stroke Scale(NIHSS),Acute Physiology and Chronic Health EvaluationⅡ(APACHEⅡ),Glasgow Coma Scale(GCS),complications,prognosis,ICU stay time and mechanical ventilation time were compared between the two groups.Results:NIHSS score[control group:(19.58±3.19)points vs(37.98±5.75)points,observation group:(10.33±2.42)points vs(38.05±5.83)points]and APACHE II score[control group:(14.55±2.17)points vs(19.87±3.50)points,observation group:(8.71±2.03)points vs(20.12±3.56)points]of the two groups at 1 month after injury were significantly lower than those at admission(P<0.05),GCS score[control group:(10.78±1.66)points vs(8.03±1.34)points,observation group:(13.10±1.72)points vs(7.99±1.32)points]were significantly higher than that at admission(P<0.05).At 1 month after injury,NIHSS score[(10.33±2.42)points vs(19.58±3.19)points],APACHE II score[(8.71±2.03)points vs(14.55±2.17)points]in the observation group were significantly lower than those in the control group(P<0.05),and GCS score[(13.10±1.72)points vs(10.78±1.66)points]in the observation group was significantly higher than that in the control group(P<0.05).The proportion of hydrocephalus(2.50%vs 12.50%),total complication rate(5.00%vs 21.25%),proportion of severe disability(5.00%vs 17.50%),proportion of survival in plant man(3.75%vs 15.00%),mortality rate(2.50%vs 12.50%),ICU stay time[(5.01±1.25)d vs(8.38±2.29)D],mechanical ventilation time[(2.18±0.75)D]in observation group were lower than those in the control group,and the good rate(56.25%vs 32.50%)and the total effective rate(93.75%vs 72.50%)in the control group were significantly higher than those in the control group(P<0.05).Conclusion:Dynamic ultrasound monitoring ONSD is effective in guiding dehydration treatment of patients with moderate and severe CI,it can significantly reduce ICP and complications,improve prognosis,which is worthy of promotion and application.
文摘Objective:To observe dynamic changes of MIP-1α, MIP-1β and MCP-1 in Acute pancreatitis patients, and to evaluate the value of MIP-1α, MIP-1β and MCP-1 in acute pancreatitis severity assessment.Methods: A total of 112 cases of acute pancreatitis were divided into mild pancreatitis (MAP group, 44 cases), moderate severe acute pancreatitis group (MSAP group, 36 cases) and severe acute pancreatitis group (SAP group, 32 cases). Serum MIP-1α, MIP-1βand MCP-1 levels were detected by enzyme-linked immunosorbent assay in patients at 1st, 4th day, 7th days.Results:(1) In 1st day, Serum concentrations of MIP-1α, MIP-1β and MCP-1 were significantly increased in three Groups, There were significant differences between the control group, MAP, MSAP and SAP group. (2) Serum concentrations of MIP-1α, MIP-1β and MCP-1 reached the peak level on 4th day in MAP group and were significantly higher than the level of those on 1st day. In the MSAP and SAP group, serum concentration of MIP-1α, MIP-1β and MCP-1 reached the peak level on 1st day, and significantly higher than the level of those on the 4th day and 7th day. There was a significant difference between MSAP group and MAP group, SAP group and MSAP group in the serum concentration of MIP-1α, MIP-1β and MCP-1 at each monitoring time.Conclusions:MIP-1α, MIP-1β and MCP-1 levels have the rule of dynamic change in Patients with acute pancreatitis, which are useful in evaluating the severity of the patients with acute pancreatitis.
基金supported by the National Natural Science Foundation of China (Nos50971046,50771037,and 50371020)the Doctoral Fund of the Ministry of Education of China (No200805620004)
文摘The Zr62.55Cu17.55Ni9.9Al10 bulk metallic glass （BMG） was prepared by using copper-mold suction-casting. X-ray diffraction and differential scanning calorimetry were utilized to determine its structure and thermal stability. Uniaxial compression and Rockwell indenta- tion tests were adopted to study the plastic deformation behavior at room temperature. The results show that the glass transition temperature and the onset temperature of exothermic reaction of the BMG are 651.5 and 748 K, respectively. During the compression test, the BMGs undergo an engineering strain of about 2.5%, i.e., true strain of 2.8%, and then fracture. The BMGs deform via the formation and propagation of shear bands. Under indentation loading, the BMGs deform through the formation of radiation-like and circular shear bands. The circular shear bands form earlier than the radiation-like ones, The formation mechanism of shear bands in the BMGs was analyzed and discussed.
基金Hainan provincial health and family planning industry research project(1801032061A2004)
文摘Objective: To explore the effect of clinically available crystalloids on lactate clearance in patients with septic shock Methods: Sixty-eight patients with septic shock who were treated in our hospital from January 2017 to January 2019 were enrolled in the study. The selected patients were randomly divided into three groups: sodium lactate Ringer's solution group (LR group), saline group (NS group) and compound sodium chloride injection group (RS group), each group received the corresponding crystal liquid for fluid resuscitation, compare three groups Heart rate (HR), mean arterial pressure (MAP), cardiac output, procalcitonin (PCT), and C-reactive protein (CRP) before and after treatment, compared with the treatment of 24 hours of lactate clearance and APACHE II scores, and the overall hospitalization time and mortality rate of the three groups of patients were statistically compared;Results: (1) The difference of HR, MAP and cardiac output between the three groups before treatment was not statistically significant (P>0.05), treatment for 24h The HR of the post-Lactate Ringer's solution group was lower than that of the control group, and the MAP and cardiac output were higher than the control group (P<0.05). (2) The difference of PCT, CRP and BNP levels between the three groups before and after treatment was not statistically significant. (P>0.05);(3) Compared with the results, there was no statistically significant difference in the clearance rate of lactate clearance between the three groups (P>0.05). Three groups before and after treatment APACHE II score was not statistically significant difference comparison (P> 0.05). (4) Three groups The overall hospitalization time and mortality rate of the patients were not statistically significant (P>0.05). Conclusion: Lactate Ringer's solution can better maintain the circulation capacity and blood pressure, which can alleviate the clinical symptoms of patients with septic shock, but no obvious effect on lactic acid clearance. The effect of resuscitation with clinically available crystalloid sodium lactate Ringer's solution, saline and compound sodium chloride injection was not statistically significant in patients with septic shock.
基金National Natural Science Foundation of China(81801894,81760341)Basic Science and Frontier Technology Research Project of Chongqing Science and Technology Commission(cstc 2016jcyjA0005)+1 种基金Science and Technology Research Project of Chongqing Education Commission(KJ1702034)Traditional Chinese Medicine Science and Technology Project of Chongqing Health and Family Planning Commission(ZY201702071).
文摘Interleukin (IL) 33 is a key cytokine in type II immune and airway diseases. It is abundantly expressed in lung epithelial cells and plays an important role in both innate and adaptive immunity. In innate immunity, IL-33 responds promptly to produce an immune response that maintains homeostasis. In adaptive immunity, IL-33 interacts with various immune cells. At the same time, IL-33 also plays an important role in chronic inflammation of the airway and its remodeling. This article reviews the relevant biological knowledge of IL-33 and its research progress in lung immunity, and discusses the related issues of IL-33 as a lung immune test site and therapeutic target.
文摘Objective:To investigate the clinical effects of continuous plasma filtration with adsorption and continuous hematodialysis on improving the immune level of patients with sepsis in ICU. Methods:A total of 80 patients with sepsis were randomly divided into study group and control group, 40 patients in each group. All the patients were treated by routine treatment. Patients in study group were treated by continuous plasma filtration with adsorption and patients in control group were treated by continuous hematodialysis. The blood samples were collected before and after treatment. The immune cell level, inflammatory factors level, APACHEⅡ score and death rate were compared between two groups.Results:All immune cell indexes were significantly improved in both groups. All immune cell indexes in study group were significantly better than those in control group, after treatment. All inflammatory factors were significantly decreased in both groups. All inflammatory factors in study group were significantly better than those in control group, after treatment. The APACHEⅡ in study group was significantly better than that in control group, after treatment. The death rate in study group was significantly less than that in control group.Conclusion:Continuous plasma filtration with adsorption has a great clinical efficacy on patients with sepsis in ICU, which can improve the immune level and prognosis, makes it worth for clinical application.
文摘Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: A total of 50 patients with gastric cancer, and 50 healthy persons were selected as the experimental group, benign gastric disease group and healthy group, respectively. A total of 24 patients in experimental group met surgery indications, and agreed to perform gastrectomy. They were divided into preoperative group and postoperative group. The serums levels of KLK7 and E-cad were detected by enzyme-linked immunosorbent assay.Results: The serum levels of KLK7 and E-cad were significantly higher than that of benign gastric disease group and normal control group. Compared with the experimental group after operation, MMP-2 and MMP-9 were decreased, KLK7 was significantly decreased and E-cad increased significantly after operation. Serum levels of KLK7 and E-cad in experimental group stage I were increased and stage IV decreased. Microvascular invasion was significantly correlated with serum KLK7 and E-cad. There was no significant correlation between tumor size, differentiation degree, depth and expression of KLK7 and E-cad. Serum KLK7 were significantly negatively correlated with E-cad expression in gastric cancer patients. Conclusions:KLK7 and E-cad are involved in the development and metastasis of gastric cancer. The serum expression of KLK7 and E-cad are significantly negatively correlated in patients with stomach cancer. There may be an antagonistic effect in gastric cancer development and metastasis. Serum level of KLK7 and E-cad can be used as indexes for progress monitoring.
