Objective:To observe the clinical manifestations of allergic rhinitis mice and the expression changes of the eosinophils CCR3 and the granule protein rnRNA in the bone marrow,peripheral blood and nasal lavage fluid.Mc...Objective:To observe the clinical manifestations of allergic rhinitis mice and the expression changes of the eosinophils CCR3 and the granule protein rnRNA in the bone marrow,peripheral blood and nasal lavage fluid.Mctliods:Twenty-four BALB/c mice were randomly divided into the control group.PBS therapy group.siKNA therapy group and the CCR3 siRNA therapy group(n=6).Allergic rhinitis model were sensitized and stimulated by ovalbunfin,and CCR3 siKNA therapy group were administered with CCH3 transnasally before stimulated.The levels of the eosinophils CCR3.MBP.ECP and EPO in bone marrow,peripheral blood and nasal lavage fluid were detected by RT-PCR.Results:Compared to the control group and CCR3 siR.NA therapy group,the nasal mucosa of the PBS therapy group and siRNA therapy group developed epithalaxy.goblet cells hyperplasia,squamous epithelium metaplasia,epithelium necrosis,lamina propria and submucosa gland hyperplasia,vasodilatation,tissue edema,and the characterized eosinophil infiltration.RT-PCR indicated that the CCR3 rnRNA,MBP.ECP and EPC)expression in bone marrow,peripheral blood and nasal lavage fluid of the CCR3 siKNA therapy group was lower than the PBS therapy group and siR.NA therapy group(P<0.05).Conclusions:The RNA interference therapy to CCR3 by local administration pernasal can suppress the process of the development,migration and invasion of the allergic rhinitis eosinophil,thus can reduce the effect of eosinophils and then reduce the inflammation effect of the allergic rhinitis.It may be a new treatment for respiratory tract allergic inflammation.展开更多
This paper proposes a simplified analytical solution considering non-Darcian and wellbore storage effect to investigate the pumping flow in a confined aquifer with barrier and recharge boundaries.The mathematical mode...This paper proposes a simplified analytical solution considering non-Darcian and wellbore storage effect to investigate the pumping flow in a confined aquifer with barrier and recharge boundaries.The mathematical modelling for the pumping-induced flow in aquifers with different boundaries is developed by employing image-well theory with the superposition principle,of which the non-Darcian effect is characterized by Izbash’s equation.The solutions are derived by Boltzmann and dimensionless transformations.Then,the non-Darcian effect and wellbore storage are especially investigated according to the proposed solution.The results show that the aquifer boundaries have non-negligible effects on pumping,and ignoring the wellbore storage can lead to an over-estimation of the drawdown in the first 10 minutes of pumping.The higher the degree of non-Darcian,the smaller the drawdown.展开更多
基金supported by the National Natural Science Fund Project in China(grant No.:81060084)Jiangxi Provincial Natural Science Fund Project in China(grant No.:2010gzy0251)+1 种基金Jiangxi Provincial Health Department Project in China(grant No.:20131059)Jiangxi Provincial Department of Science and Technology Project in China(grant No.:20133BBG70071)
文摘Objective:To observe the clinical manifestations of allergic rhinitis mice and the expression changes of the eosinophils CCR3 and the granule protein rnRNA in the bone marrow,peripheral blood and nasal lavage fluid.Mctliods:Twenty-four BALB/c mice were randomly divided into the control group.PBS therapy group.siKNA therapy group and the CCR3 siRNA therapy group(n=6).Allergic rhinitis model were sensitized and stimulated by ovalbunfin,and CCR3 siKNA therapy group were administered with CCH3 transnasally before stimulated.The levels of the eosinophils CCR3.MBP.ECP and EPO in bone marrow,peripheral blood and nasal lavage fluid were detected by RT-PCR.Results:Compared to the control group and CCR3 siR.NA therapy group,the nasal mucosa of the PBS therapy group and siRNA therapy group developed epithalaxy.goblet cells hyperplasia,squamous epithelium metaplasia,epithelium necrosis,lamina propria and submucosa gland hyperplasia,vasodilatation,tissue edema,and the characterized eosinophil infiltration.RT-PCR indicated that the CCR3 rnRNA,MBP.ECP and EPC)expression in bone marrow,peripheral blood and nasal lavage fluid of the CCR3 siKNA therapy group was lower than the PBS therapy group and siR.NA therapy group(P<0.05).Conclusions:The RNA interference therapy to CCR3 by local administration pernasal can suppress the process of the development,migration and invasion of the allergic rhinitis eosinophil,thus can reduce the effect of eosinophils and then reduce the inflammation effect of the allergic rhinitis.It may be a new treatment for respiratory tract allergic inflammation.
基金supported by the National Natural Science Foundation of China (Grant Numbers41807197, 2017YFC0405900, and 51469002)the Natural Science Foundation of Guangxi (Grant Numbers 2017GXNSFBA198087, 2018GXNSFAA138042, and GuiKeAB17195073)Hebei Highlevel Talent Funding Project (B2018003016)。
文摘This paper proposes a simplified analytical solution considering non-Darcian and wellbore storage effect to investigate the pumping flow in a confined aquifer with barrier and recharge boundaries.The mathematical modelling for the pumping-induced flow in aquifers with different boundaries is developed by employing image-well theory with the superposition principle,of which the non-Darcian effect is characterized by Izbash’s equation.The solutions are derived by Boltzmann and dimensionless transformations.Then,the non-Darcian effect and wellbore storage are especially investigated according to the proposed solution.The results show that the aquifer boundaries have non-negligible effects on pumping,and ignoring the wellbore storage can lead to an over-estimation of the drawdown in the first 10 minutes of pumping.The higher the degree of non-Darcian,the smaller the drawdown.
基金Project supported by the National High-Tech R&D Program(863 Program)of China(No.2014AA0415011)the Research Fund for the Doctoral Program of Higher Education of China(No.2014QD02S)+1 种基金the Research Fund for the Doctoral Program of Higher Education of China(No.2013QD03S)the Fundamental Research Funds for the Central Universities(No.3122015C015),China
