BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patie...BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.展开更多
After completion of the human genome project, health professionals and the public become more aware aboutthe role of genetic information in medical practice. We are in a transition period to enter a new“genomic medi-...After completion of the human genome project, health professionals and the public become more aware aboutthe role of genetic information in medical practice. We are in a transition period to enter a new“genomic medi-cine”era[1,2]. In the past two decades, many mutations at disease causing genes have been identified and correla-ted to clinical phenotypes. Molecular diagnostic tests for such mutations have been developed and shown their pro-found impacts on clinical medicine and public health. With the development of new genomic technologies, large-scale analysis of human genome and transcriptome are become possible. For example,microarray analysis allows as-sessment of entire expression profile of cancer. By integrating of information from genome and expression profileswith clinical data, cancer molecular classification will become a powerful clinical tool to correlation cancers withtheir prognostics, sensitivity to chemotherapy, and the potential of metastasis[3]. Thus, molecular diagnosis will be-come important soon for clinical physicians and cancer patients on selection of the optimal healthcare approaches.展开更多
This paper deals with an improved bonding approach of surface-bonded fiber Bragg grating (FBG) sensors for airship envelope structural health monitoring (SHM) under the strain transfer theory. A theoretical formula is...This paper deals with an improved bonding approach of surface-bonded fiber Bragg grating (FBG) sensors for airship envelope structural health monitoring (SHM) under the strain transfer theory. A theoretical formula is derived from the proposed model to predict the strain transfer relationship between the airship envelope and fiber core. Then theoretical predictions are validated by numerical analysis using the finite element method (FEM). Finally, on the basis of the theoretical approach and numerical validation, parameters that influence the strain transfer rate from the airship envelope to fiber core and the ratio of effective sensing length are analyzed, and some meaningful conclusions are provided.展开更多
Objective:Saphenous vein grafts disease(SVGD)is a common complication after coronary artery bypass graft(CABG)and normally treated by percutaneous coronary intervention(PCI).The most common complication after SVG-PCI ...Objective:Saphenous vein grafts disease(SVGD)is a common complication after coronary artery bypass graft(CABG)and normally treated by percutaneous coronary intervention(PCI).The most common complication after SVG-PCI is slow or no-reflow.It is known that the no-reflow phenomenon occurs in up to 15%of the SVG-PCI and is associated with high risk of major adverse cardiac events(MACEs)and mortality,therefore,it is important to investigate the factors that could predict the clinical outcome of PCI for risk stratification and guiding interventions.In recent years,the spectral analysis of intravascular ultrasound(IVUS)radiofrequency data(virtual histology-IVUS[VH-IVUS])has been used to provide quantitative assessment on both plaque compositions and morphologic characteristics.Data sources:The PubMed,Embase,and Central databases were searched for possible relevant studies published from 1997 to 2018 using the following index keywords:“Coronary artery bypass grafting,”“Saphenous venous graft disease,”“Virtual histologyintravascular ultrasound,”“Virtual histology-intravascular ultrasound,”and“Percutaneous coronary intervention.”Study selection:The primary references were Chinese and English articles including original studies and literature reviews,were identified and reviewed to summarize the advances in the application of VH-IVUS techniques in situ vascular and venous graft vascular lesions.Results:With different plaque components exhibiting a defined spectrum,VH-IVUS can classify atherosclerotic plaque into four types:fibrous tissue(FT),fibro fatty(FF),necrotic core(NC),and dense calcium(DC).The radiofrequency signal is mathematically transformed into a color-coded representation,including lipid,fibrous tissue,calcification,and necrotic core.Several studies have demonstrated the independent relationship between VH-IVUS-defined plaque classification or plaque composition and MACEs,but a significant association between plaque components and no-reflow after PCI in acute coronary syndrome.In recent years,VH-IVUS are applied to assess the plaque composition of SVGD,based on the similarity of pathophysiological mechanisms between coronary artery disease(CAD)and SVGD,further studies with the larger sample size,the long-term follow-up,multicenter clinical trials may be warranted to investigate the relationship between plaque composition of saphenous vein graft(SVG)by VH-IVUS and clinical outcomes in patients with SVGD undergoing PCI.Conclusions:In degenerative SVG lesions,VH-IVUS found that plaque composition was associated with clinical features,future studies need to explore the relationship between VH-IVUS defined atherosclerotic plaque components and clinical outcomes in SVGD patients undergoing PCI,an innovative prediction tool of clinical outcomes can be created.展开更多
β-cryptoxanthin （CRY）, a major carotenoid of potential interest for health, is obtained naturally from orange vegetables and fruits. A few research studies have reported that CRY could decrease oxidative stress and...β-cryptoxanthin （CRY）, a major carotenoid of potential interest for health, is obtained naturally from orange vegetables and fruits. A few research studies have reported that CRY could decrease oxidative stress and germ cell apoptosis. The purpose of this study was to examine the effects of CRY on acute cadmium chloride （CdCl2）-induced oxidative damage in rat testes. For this study, 24 rats were divided into four groups, one of which serves as a control group that received intraperitoneal （i.p.） injections of corn oil and physiological saline. The other rats were i.p. injected with CRY （10 μg kg^-1） every 8 h, beginning 8 h before CdCI2 （2.0 mg kg^-1） treatment. The pathological and TUNEL findings revealed that CRY ameliorated the Cd-induced testicular histological changes and germ cell apoptosis in the rats, Furthermore, the Cd-induced decrease in the testicular testosterone （T） level was attenuated after CRY administration （P 〈 0.05）. The administration of CRY significantly reversed the Cd-induced increases in the lipid peroxide （LPO） and malondialdehyde （MDA） levels （P 〈 0.01）. The testicular antioxidants superoxide dismutase （SOD）, catalase （CAT） and glutathione （GSH） were decreased by treatment with Cd alone but were restored by CRY co-treatment. These results demonstrated that the application of CRY can enhance the tolerance of rats to Cd-induced oxidative damage and suggest that it has promised as a pharmacological agent to protect against Cd-induced testicular toxicity.展开更多
Numerical simulation of two-phase flow in fractured karst reservoirs is still a challenging issue.The triple-porosity model is the major approach up to now.However,the triple-continuum assumption in this model is unac...Numerical simulation of two-phase flow in fractured karst reservoirs is still a challenging issue.The triple-porosity model is the major approach up to now.However,the triple-continuum assumption in this model is unacceptable for many cases.In the present work,an efficient numerical model has been developed for immiscible two-phase flowin fractured karst reservoirs based on the idea of equivalent continuum representation.First,based on the discrete fracture-vug model and homogenization theory,the effective absolute permeability tensors for each grid blocks are calculated.And then an analytical procedure to obtain a pseudo relative permeability curves for a grid block containing fractures and cavities has been successfully implemented.Next,a full-tensor simulator has been designed based on a hybrid numerical method(combining mixed finite element method and finite volume method).A simple fracture system has been used to demonstrate the validity of our method.At last,we have used the fracture and cavity statistics data fromTAHE outcrops in west China,effective permeability values and other parameters from our code,and an equivalent continuum simulator to calculate the water flooding profiles for more realistic systems.展开更多
This paper is concerned with the stochastic stability and passivity analysis for a class of Lur’e singular systems with time-varying delay and Markovian switching. By using the free-weighting matrices approach, a del...This paper is concerned with the stochastic stability and passivity analysis for a class of Lur’e singular systems with time-varying delay and Markovian switching. By using the free-weighting matrices approach, a delay-dependent stability criterion, which guarantees that the system is stochastically stable and robustly passive, is derived in terms of linear matrix inequality （LMI）. Two numerical examples are provided to illustrate the effectiveness of the proposed method. 更多还原展开更多
基金Supported by the National Natural Science Foundation of China,No.81900743Heilongjiang Province Outstanding Young Medical Talents Training Grant Project,China,No.HYD2020YQ0007.
文摘BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.
文摘After completion of the human genome project, health professionals and the public become more aware aboutthe role of genetic information in medical practice. We are in a transition period to enter a new“genomic medi-cine”era[1,2]. In the past two decades, many mutations at disease causing genes have been identified and correla-ted to clinical phenotypes. Molecular diagnostic tests for such mutations have been developed and shown their pro-found impacts on clinical medicine and public health. With the development of new genomic technologies, large-scale analysis of human genome and transcriptome are become possible. For example,microarray analysis allows as-sessment of entire expression profile of cancer. By integrating of information from genome and expression profileswith clinical data, cancer molecular classification will become a powerful clinical tool to correlation cancers withtheir prognostics, sensitivity to chemotherapy, and the potential of metastasis[3]. Thus, molecular diagnosis will be-come important soon for clinical physicians and cancer patients on selection of the optimal healthcare approaches.
基金Project (No. 2011AA7052011) supported by the National High-Tech R&D (863) Program of China
文摘This paper deals with an improved bonding approach of surface-bonded fiber Bragg grating (FBG) sensors for airship envelope structural health monitoring (SHM) under the strain transfer theory. A theoretical formula is derived from the proposed model to predict the strain transfer relationship between the airship envelope and fiber core. Then theoretical predictions are validated by numerical analysis using the finite element method (FEM). Finally, on the basis of the theoretical approach and numerical validation, parameters that influence the strain transfer rate from the airship envelope to fiber core and the ratio of effective sensing length are analyzed, and some meaningful conclusions are provided.
文摘Objective:Saphenous vein grafts disease(SVGD)is a common complication after coronary artery bypass graft(CABG)and normally treated by percutaneous coronary intervention(PCI).The most common complication after SVG-PCI is slow or no-reflow.It is known that the no-reflow phenomenon occurs in up to 15%of the SVG-PCI and is associated with high risk of major adverse cardiac events(MACEs)and mortality,therefore,it is important to investigate the factors that could predict the clinical outcome of PCI for risk stratification and guiding interventions.In recent years,the spectral analysis of intravascular ultrasound(IVUS)radiofrequency data(virtual histology-IVUS[VH-IVUS])has been used to provide quantitative assessment on both plaque compositions and morphologic characteristics.Data sources:The PubMed,Embase,and Central databases were searched for possible relevant studies published from 1997 to 2018 using the following index keywords:“Coronary artery bypass grafting,”“Saphenous venous graft disease,”“Virtual histologyintravascular ultrasound,”“Virtual histology-intravascular ultrasound,”and“Percutaneous coronary intervention.”Study selection:The primary references were Chinese and English articles including original studies and literature reviews,were identified and reviewed to summarize the advances in the application of VH-IVUS techniques in situ vascular and venous graft vascular lesions.Results:With different plaque components exhibiting a defined spectrum,VH-IVUS can classify atherosclerotic plaque into four types:fibrous tissue(FT),fibro fatty(FF),necrotic core(NC),and dense calcium(DC).The radiofrequency signal is mathematically transformed into a color-coded representation,including lipid,fibrous tissue,calcification,and necrotic core.Several studies have demonstrated the independent relationship between VH-IVUS-defined plaque classification or plaque composition and MACEs,but a significant association between plaque components and no-reflow after PCI in acute coronary syndrome.In recent years,VH-IVUS are applied to assess the plaque composition of SVGD,based on the similarity of pathophysiological mechanisms between coronary artery disease(CAD)and SVGD,further studies with the larger sample size,the long-term follow-up,multicenter clinical trials may be warranted to investigate the relationship between plaque composition of saphenous vein graft(SVG)by VH-IVUS and clinical outcomes in patients with SVGD undergoing PCI.Conclusions:In degenerative SVG lesions,VH-IVUS found that plaque composition was associated with clinical features,future studies need to explore the relationship between VH-IVUS defined atherosclerotic plaque components and clinical outcomes in SVGD patients undergoing PCI,an innovative prediction tool of clinical outcomes can be created.
文摘β-cryptoxanthin （CRY）, a major carotenoid of potential interest for health, is obtained naturally from orange vegetables and fruits. A few research studies have reported that CRY could decrease oxidative stress and germ cell apoptosis. The purpose of this study was to examine the effects of CRY on acute cadmium chloride （CdCl2）-induced oxidative damage in rat testes. For this study, 24 rats were divided into four groups, one of which serves as a control group that received intraperitoneal （i.p.） injections of corn oil and physiological saline. The other rats were i.p. injected with CRY （10 μg kg^-1） every 8 h, beginning 8 h before CdCI2 （2.0 mg kg^-1） treatment. The pathological and TUNEL findings revealed that CRY ameliorated the Cd-induced testicular histological changes and germ cell apoptosis in the rats, Furthermore, the Cd-induced decrease in the testicular testosterone （T） level was attenuated after CRY administration （P 〈 0.05）. The administration of CRY significantly reversed the Cd-induced increases in the lipid peroxide （LPO） and malondialdehyde （MDA） levels （P 〈 0.01）. The testicular antioxidants superoxide dismutase （SOD）, catalase （CAT） and glutathione （GSH） were decreased by treatment with Cd alone but were restored by CRY co-treatment. These results demonstrated that the application of CRY can enhance the tolerance of rats to Cd-induced oxidative damage and suggest that it has promised as a pharmacological agent to protect against Cd-induced testicular toxicity.
基金supported by the National Basic Research Program of China(“973”Program)(Grant No.2011CB201004)the ImportantNational Science and Technology Project of China(Grant No.2011ZX05014-005-003HZ)+2 种基金the National Natural Science Foundation of China(Grant No.11102237)the Introducing Talents of Discipline to Universities of China(Grant No.B08028)the Fundamental Research Funds for the Central Universities(Grant No.27R1102065A).
文摘Numerical simulation of two-phase flow in fractured karst reservoirs is still a challenging issue.The triple-porosity model is the major approach up to now.However,the triple-continuum assumption in this model is unacceptable for many cases.In the present work,an efficient numerical model has been developed for immiscible two-phase flowin fractured karst reservoirs based on the idea of equivalent continuum representation.First,based on the discrete fracture-vug model and homogenization theory,the effective absolute permeability tensors for each grid blocks are calculated.And then an analytical procedure to obtain a pseudo relative permeability curves for a grid block containing fractures and cavities has been successfully implemented.Next,a full-tensor simulator has been designed based on a hybrid numerical method(combining mixed finite element method and finite volume method).A simple fracture system has been used to demonstrate the validity of our method.At last,we have used the fracture and cavity statistics data fromTAHE outcrops in west China,effective permeability values and other parameters from our code,and an equivalent continuum simulator to calculate the water flooding profiles for more realistic systems.
基金supported by National High Technology Research and Development Program of China (863 Program)(No. 2011AA7052011)
文摘This paper is concerned with the stochastic stability and passivity analysis for a class of Lur’e singular systems with time-varying delay and Markovian switching. By using the free-weighting matrices approach, a delay-dependent stability criterion, which guarantees that the system is stochastically stable and robustly passive, is derived in terms of linear matrix inequality （LMI）. Two numerical examples are provided to illustrate the effectiveness of the proposed method. 更多还原
