The properties of an atmospheric-pressure collisional plasma sheath with nonextensively distributed electrons and hypothetical ionization source terms are studied in this work. The Bohm criterion for the magnetized pl...The properties of an atmospheric-pressure collisional plasma sheath with nonextensively distributed electrons and hypothetical ionization source terms are studied in this work. The Bohm criterion for the magnetized plasma is extended in the presence of an ion–neutral collisional force and ionization source. The effects of electron nonextensive distribution, ionization frequency, ion–neutral collision, magnetic field angle and ion temperature on the Bohm criterion of the plasma sheath are numerically analyzed. The fluid equations are solved numerically in the plasma–wall transition region using a modified Bohm criterion as the boundary condition. The plasma sheath properties such as charged particle density, floating sheath potential and thickness are thoroughly investigated under different kinds of ion source terms, contributions of collisions, and magnetic fields. The results show that the effect of the ion source term on the properties of atmosphericpressure collisional plasma sheath is significant. As the ionization frequency increases, the Mach number of the Bohm criterion decreases and the range of possible values narrows. When the ion source is considered, the space charge density increases, the sheath potential drops more rapidly,and the sheath thickness becomes narrower. In addition, ion–neutral collision, magnetic field angle and ion temperature also significantly affect the sheath potential profile and sheath thickness.展开更多
The secondary electron emission(SEE) and inclined magnetic field are typical features at the channel wall of the Hall thruster acceleration region(AR), and the characteristics of the magnetized sheath have a significa...The secondary electron emission(SEE) and inclined magnetic field are typical features at the channel wall of the Hall thruster acceleration region(AR), and the characteristics of the magnetized sheath have a significant effect on the radial potential distribution, ion radial acceleration and wall erosion. In this work, the magnetohydrodynamics model is used to study the characteristics of the magnetized sheath with SEE in the AR of Hall thruster. The electrons are assumed to obey non-extensive distribution, the ions and secondary electrons are magnetized.Based on the Sagdeev potential, the modified Bohm criterion is derived, and the influences of the non-extensive parameter and magnetic field on the AR sheath structure and parameters are discussed. Results show that, with the decrease of the parameter q, the high-energy electron leads to an increase of the potential drop in the sheath, and the sheath thickness expands accordingly,the kinetic energy rises when ions reach the wall, which can aggravate the wall erosion.Increasing the magnetic field inclination angle in the AR of the Hall thruster, the Lorenz force along the x direction acting as a resistance decelerating ions becomes larger which can reduce the wall erosion, while the strength of magnetic field in the AR has little effect on Bohm criterion and wall potential. The propellant type also has a certain effect on the values of wall potential,secondary electron number density and sheath thickness.展开更多
UBE2C(Ubiquitin conjugating enzyme E2 C), a key regulator of cell cycle progression, is a promising target for discovery of antitumor agents. However, it is challenging to develop inhibitors of UBE2C owing to its lack...UBE2C(Ubiquitin conjugating enzyme E2 C), a key regulator of cell cycle progression, is a promising target for discovery of antitumor agents. However, it is challenging to develop inhibitors of UBE2C owing to its lack of “druggable” pockets. Bio PROTACs(biological proteolysis targeting chimeras) are a kind of protein-based degraders by fusing an adaptor to a subunit of E3 ligase for ubiquitination and subsequent proteasome-dependent degradation of target protein. We report herein the design and biological evaluation of a UBE2C-targeting bio PROTAC based on the NEL(novel E3 ligase) domain of bacterial E3 ligase Ipa H9.8 and the UBE2C-binding WHB(winged-helix B) domain of APC_(2)(anaphase promoting complex subunit 2). The in vitro ubiquitination test and Mass Spectrometry analysis showed that the bio PROTAC could transfer ubiquitin to surface exposed lysines on UBE2C and catalyzed the formation of polyubiquitin chains. In addition, the transient co-expression experiment showed that the bio PROTAC could promote proteasomal degradation of heterologous UBE2C and rescue its downstream substrates in mammalian cells.展开更多
基金supported by National Natural Science Foundation of China(Nos.11975062,11605021 and 11975088)the China Postdoctoral Science Foundation(No.2017M621120)。
文摘The properties of an atmospheric-pressure collisional plasma sheath with nonextensively distributed electrons and hypothetical ionization source terms are studied in this work. The Bohm criterion for the magnetized plasma is extended in the presence of an ion–neutral collisional force and ionization source. The effects of electron nonextensive distribution, ionization frequency, ion–neutral collision, magnetic field angle and ion temperature on the Bohm criterion of the plasma sheath are numerically analyzed. The fluid equations are solved numerically in the plasma–wall transition region using a modified Bohm criterion as the boundary condition. The plasma sheath properties such as charged particle density, floating sheath potential and thickness are thoroughly investigated under different kinds of ion source terms, contributions of collisions, and magnetic fields. The results show that the effect of the ion source term on the properties of atmosphericpressure collisional plasma sheath is significant. As the ionization frequency increases, the Mach number of the Bohm criterion decreases and the range of possible values narrows. When the ion source is considered, the space charge density increases, the sheath potential drops more rapidly,and the sheath thickness becomes narrower. In addition, ion–neutral collision, magnetic field angle and ion temperature also significantly affect the sheath potential profile and sheath thickness.
基金supported by National Natural Science Foundation of China (Nos. 11975062, 11605021, 11975088)the China Postdoctoral Science Foundation (No. 2017M621120)。
文摘The secondary electron emission(SEE) and inclined magnetic field are typical features at the channel wall of the Hall thruster acceleration region(AR), and the characteristics of the magnetized sheath have a significant effect on the radial potential distribution, ion radial acceleration and wall erosion. In this work, the magnetohydrodynamics model is used to study the characteristics of the magnetized sheath with SEE in the AR of Hall thruster. The electrons are assumed to obey non-extensive distribution, the ions and secondary electrons are magnetized.Based on the Sagdeev potential, the modified Bohm criterion is derived, and the influences of the non-extensive parameter and magnetic field on the AR sheath structure and parameters are discussed. Results show that, with the decrease of the parameter q, the high-energy electron leads to an increase of the potential drop in the sheath, and the sheath thickness expands accordingly,the kinetic energy rises when ions reach the wall, which can aggravate the wall erosion.Increasing the magnetic field inclination angle in the AR of the Hall thruster, the Lorenz force along the x direction acting as a resistance decelerating ions becomes larger which can reduce the wall erosion, while the strength of magnetic field in the AR has little effect on Bohm criterion and wall potential. The propellant type also has a certain effect on the values of wall potential,secondary electron number density and sheath thickness.
基金supported by the National Natural Science Foundation of China (No. 21907006 to Yuxin Ye)the Natural Science Foundation of Guangdong Province (No. 2020A1515011544to Yuxin Ye)the Shenzhen Science and Technology Innovation Committee (No. JCYJ20180302150357309 to Yuxin Ye, and No.JCYJ20200109140401752 to Hao Huang)。
文摘UBE2C(Ubiquitin conjugating enzyme E2 C), a key regulator of cell cycle progression, is a promising target for discovery of antitumor agents. However, it is challenging to develop inhibitors of UBE2C owing to its lack of “druggable” pockets. Bio PROTACs(biological proteolysis targeting chimeras) are a kind of protein-based degraders by fusing an adaptor to a subunit of E3 ligase for ubiquitination and subsequent proteasome-dependent degradation of target protein. We report herein the design and biological evaluation of a UBE2C-targeting bio PROTAC based on the NEL(novel E3 ligase) domain of bacterial E3 ligase Ipa H9.8 and the UBE2C-binding WHB(winged-helix B) domain of APC_(2)(anaphase promoting complex subunit 2). The in vitro ubiquitination test and Mass Spectrometry analysis showed that the bio PROTAC could transfer ubiquitin to surface exposed lysines on UBE2C and catalyzed the formation of polyubiquitin chains. In addition, the transient co-expression experiment showed that the bio PROTAC could promote proteasomal degradation of heterologous UBE2C and rescue its downstream substrates in mammalian cells.
