This article examines the soft soil roadbed reinforcement technology for widened sections of highways in a specific project.It provides an overview of the project,the principles of soft soil roadbed reinforcement tech...This article examines the soft soil roadbed reinforcement technology for widened sections of highways in a specific project.It provides an overview of the project,the principles of soft soil roadbed reinforcement technology for wide sections,and its practical application.The analysis aims to offer guidance on applying soft soil roadbed wide section reinforcement technology and enhancing the overall quality of similar projects.展开更多
In this paper,the geological condition of the right-side slope of the K114+694–K115+162 section of Yong-tai-wen Expressway is investigated and analyzed with the results showing that the strength of rock mass is the m...In this paper,the geological condition of the right-side slope of the K114+694–K115+162 section of Yong-tai-wen Expressway is investigated and analyzed with the results showing that the strength of rock mass is the main contributor to the stability of the slope.Then,two widening schemes are proposed,which are the steep slope with strong support and the gentle slope with general support schemes.The static/slope module of MIDAS GTS finite element analysis software and the strength reduction method were used to compare the two schemes.The results show that the steep slope with a strong support scheme has obvious advantages in land requisition,environmental protection,and safety and is more suitable for reconstructing and expanding the highway slope.展开更多
Objectives:To assess whether the extension of the flushing interval will increase risks of complications associated with totally implantable venous access port(TIVAP)in the off-treatment period.Methods:A retrospective...Objectives:To assess whether the extension of the flushing interval will increase risks of complications associated with totally implantable venous access port(TIVAP)in the off-treatment period.Methods:A retrospective single-center observational study was performed.Patients with a TIVAP in the off-treatment period that underwent regular flushing in our clinic were included.Data concerning patients and their TIVAPs were recorded.Patient baseline characteristics and TIVAP-related complications were analyzed.Continuous variables were analyzed by ANOVA or the Kruskal-Wallis H test.To compare the occurrence of TIVAP-related complications,the chi-square test was used;if needed,Fisher’s exact test was used.Results:Totally 607 patients were reviewed,and 563 patients were finally included.Thirteen complications were recorded,including 11 cases of catheter occlusion(1.95%),one case of port cannula rotation(0.18%),and one case of catheter tip malposition(0.18%).No device-related infection or venous thrombosis was recorded.Among these patients,the average flushing interval was 35.27±13.09 days.Patients were divided into three groups according to the flushing interval:every 28 days or less(Group 1,n=133);every 29-44 days(Group 2,n=350);and every 45 days or more(Group 3,n=80).No significant difference in catheter-related complications was found among the three groups(P>0.05).Conclusions:In the TIVAP off-treatment period,patients without any history of TIVAP-related complications during approximately one year can attempt to prolong the flushing interval to more than 4 weeks;we further suggest that 5-6 weeks may be an appropriate option for these patients.展开更多
BACKGROUND Changes in China's fertility policy have led to a significant increase in older pregnant women.At present,there is a lack of analysis of influencing factors and research on predictive models for postpar...BACKGROUND Changes in China's fertility policy have led to a significant increase in older pregnant women.At present,there is a lack of analysis of influencing factors and research on predictive models for postpartum depression(PPD)in older pregnant women.AIM To analysis the influencing factors and the construction of predictive models for PPD in older pregnant women.METHODS By adopting a cross-sectional survey research design,239 older pregnant women(≥35 years old)who underwent obstetric examinations and gave birth at Suzhou Ninth People's Hospital from February 2022 to July 2023 were selected as the research subjects.When postpartum women of advanced maternal age came to the hospital for follow-up 42 d after birth,the Edinburgh PPD Scale(EPDS)was used to assess the presence of PPD symptoms.The women were divided into a PPD group and a no-PPD group.Two sets of data were collected for analysis,and a prediction model was constructed.The performance of the predictive model was evaluated using receiver operating characteristic(ROC)analysis and the Hosmer-Lemeshow goodness-of-fit test.RESULTS On the 42nd day after delivery,51 of 239 older pregnant women were evaluated with the EPDS scale and found to have depressive symptoms.The incidence rate was 21.34%(51/239).There were statistically significant differences between the PPD group and the no-PPD group in terms of education level(P=0.004),family relationships(P=0.001),pregnancy complications(P=0.019),and mother–infant separation after birth(P=0.002).Multivariate logistic regression analysis showed that a high school education and below,poor family relationships,pregnancy complications,and the separation of the mother and baby after birth were influencing factors for PPD in older pregnant women(P<0.05).Based on the influencing factors,the following model equation was developed:Logit(P)=0.729×education level+0.942×family relationship+1.137×pregnancy complications+1.285×separation of the mother and infant after birth-6.671.The area under the ROC curve of this prediction model was 0.873(95%CI:0.821-0.924),the sensitivity was 0.871,and the specificity was 0.815.The deviation between the value predicted by the model and the actual value through the Hosmer-Lemeshow goodness-of-fit test was not statistically significant(χ^(2)=2.749,P=0.638),indicating that the model did not show an overfitting phenomenon.CONCLUSION The risk of PPD among older pregnant women is influenced by educational level,family relationships,pregnancy complications,and the separation of the mother and baby after birth.A prediction model based on these factors can effectively predict the risk of PPD in older pregnant women.展开更多
AIM: To investigate the dynamic features of insulin-like growth factor-I receptor (IGF-IR) expression in rat hepatocarcinogenesis, and the relationship between IGF-IR and hepatocytes malignant transformation at mRNA o...AIM: To investigate the dynamic features of insulin-like growth factor-I receptor (IGF-IR) expression in rat hepatocarcinogenesis, and the relationship between IGF-IR and hepatocytes malignant transformation at mRNA or protein level.METHODS: Hepatoma models were made by inducing with 2-fluorenylacetamide (2-FAA) on male Sprague-Dawley rats. Morphological changes of hepatocytes were observed by pathological Hematoxylin and eosin staining, the dynamic expressions of liver and serum IGF-IR were quantitatively analyzed by an enzyme-linked immunosorbent assay. The distribution of hepatic IGF-IR was located by immunohistochemistry. The fragments of IGF-IR gene were amplified by reverse transcription-polymerase chain reaction, and confirmed by sequencing.RESULTS: Rat hepatocytes after induced by 2-FAA were changed dynamically from granule-like degeneration, precancerous to hepatoma formation with the progressing increasing of hepatic mRNA or IGF-IR expression. The incidences of liver IGF-IR, IGF-IR mRNA, specific IGF-IR concentration (ng/mg wet liver), and serum IGF-IR level (ng/mL) were 0.0%, 0.0%, 0.63 ± 0.17, and 1.33 ± 0.47 in the control; 50.0%, 61.1%, 0.65 ± 0.2, and 1.51 ± 0.46 in the degeneration; 88.9%, 100%, 0.66 ± 0.14, and 1.92 ± 0.29 in the precancerosis; and 100%, 100%, 0.96 ± 0.09, and 2.43 ± 0.57 in the cancerous group, respectively. IGF-IR expression in the cancerous group was significantly higher (P < 0.01) than that in any of other groups at mRNA or protein level. The closely positive IGF-IR relationship was found between livers and sera (r = 0.91, t = 14.222, P < 0.01), respectively.CONCLUSION: IGF-IR expression may participate in rat hepatocarcinogenesis and its abnormality should be an early marker for hepatocytes malignant transformation.展开更多
AIM: To interfere with the activation of nuclear factor-κB(NF-κB) with metformin and explore its effect in reversing multidrug resistance(MDR) of hepatocellular carcinoma(HCC) cells.METHODS: Expression of P-glycopro...AIM: To interfere with the activation of nuclear factor-κB(NF-κB) with metformin and explore its effect in reversing multidrug resistance(MDR) of hepatocellular carcinoma(HCC) cells.METHODS: Expression of P-glycoprotein(P-gp) and NF-κB in human HepG 2 or HepG 2/adriamycin(ADM) cells treated with pC MV-NF-κB-small interference RNA(siR NA) with or without metformin, was analyzed by Western blot or fluorescence quantitative PCR. Cell viability was tested by CCK-8 assay. Cell cycle and apoptosis were measured by flow cytometry and Annexin-V-PE/7-AnnexinV apoptosis detection double staining assay, respectively. RESULTS: P-gp overexpression in HepG 2 and HepG 2/ADM cells was closely related to mdr1 mR NA(3.310 ± 0.154) and NF-κB mR NA(2.580 ± 0.040) expression. NF-κB gene transcription was inhibited by specific siR NA with significant down-regulation of P-gp and enhanced HCC cell chemosensitivity to doxorubicin. After pretreatment with metformin, Hep G2/ADM cells were sensitized to doxorubicin and P-gp was decreased through the NF-κB signaling pathway. The synergistic effect of metformin and NF-κB siR NA were found in HepG 2/ADM cells with regard to proliferation inhibition, cell cycle arrest and inducing cell apoptosis. CONCLUSION: Metformin via silencing NF-κB signaling could effectively reverse MDR of HCC cells by downregulating MDR1/P-gp expression.展开更多
A proteomic study,using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight,was conducted in apple fruit(cv.‘Golden Delicious’)starting at...A proteomic study,using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight,was conducted in apple fruit(cv.‘Golden Delicious’)starting at 10 days prior to harvest through 50 days in storage.Total protein was extracted using a phenol/sodium dodecyl sulfate protocol.More than 400 protein spots were detected in each gel and 55 differentially expressed proteins(p,0.05)were subjected to matrix-assisted laser desorption/ionization time-of-flight/time-of-flight analysis.Fifty-three of these proteins were finally identified using an apple expressed sequence tag database downloaded from Genome Database for Rosaceae and placed into six categories.The categories and the percentage of proteins placed in each category were stress response and defense(49.0%),energy and metabolism(34.0%),fruit ripening and senescence(5.6%),signal transduction(3.8%),cell structure(3.8%)and protein synthesis(3.8%).Proteins involved in several multiple metabolic pathways,including glycolysis,pentose–phosphate pathway,anti-oxidative systems,photosynthesis and cell wall synthesis,were downregulated,especially during the climacteric burst in respiration and during the senescent stages of fruit development.Proteins classified as allergens or involved in cell wall degradation were upregulated during the ripening process.Some protein spots exhibited a mixed pattern(increasing to maximal abundance followed by a decrease),such as 1-aminocyclopropane-1-carboxylate oxidase,L-ascorbate peroxidase and abscisic acid response proteins.The identification of differentially expressed proteins associated with physiological processes identified in the current study provides a baseline of information for understanding the metabolic processes and regulatory mechanisms that occur in climacteric apple fruit during ripening and senescence.展开更多
AIM: To investigate small interfering RNA(si RNA)-mediated inhibition of nuclear factor-kappa B(NF-κB) activation and multidrug-resistant(MDR) phenotype formation in human Hep G2 cells. METHODS: Total RNA was extract...AIM: To investigate small interfering RNA(si RNA)-mediated inhibition of nuclear factor-kappa B(NF-κB) activation and multidrug-resistant(MDR) phenotype formation in human Hep G2 cells. METHODS: Total RNA was extracted from human Hep G2 or LO2 cells. NF-κB/p65 m RNA was amplified by nested reverse transcription polymerase chain reaction and confirmed by sequencing. NF-κB/p65 was analyzed by immunohistochemistry. Specific-si RNA was transfected to Hep G2 cells to knock down NF-κB/p65 expression. The effects on cell proliferation, survival, and apoptosis were assessed, and the level of NF-κB/p65 or P-glycoprotein(P-gp) was quantitatively analyzed by enzyme-linked immunosorbent assay.RESULTS: Hep G2 cells express NF-κB/p65 and express relatively less phosphorylated p65(P-p65) and little P-gp. After treatment of Hep G2 cells with different doses of doxorubicin, the expression of NF-κB/p65, P-p65, and especially P-gp were dose-dependently upregulated. After Hep G2 cells were transfected with NF-κB/p65 si RNA(100 nmol/L), the expression of NF-κB/p65, P-p65, and P-gp were downregulatedsignificantly and dose-dependently. The viability of Hep G2 cells was decreased to 23% in the combination NF-κB/p65 si RNA(100 nmol/L) and doxorubicin(0.5 μmol/L) group and 47% in the doxorubicin(0.5 μmol/L) group(t = 7.043, P < 0.001). CONCLUSION: Knockdown of NF-κB/p65 with si RNA is an effective strategy for inhibiting Hep G2 cell growth by downregulating P-gp expression associated chemosensitization and apoptosis induction.展开更多
Fruit detection and counting are essential tasks for horticulture research.With computer vision technology development,fruit detection techniques based on deep learning have been widely used in modern orchards.However...Fruit detection and counting are essential tasks for horticulture research.With computer vision technology development,fruit detection techniques based on deep learning have been widely used in modern orchards.However,most deep learning-based fruit detection models are generated based on fully supervised approaches,which means a model trained with one domain species may not be transferred to another.There is always a need to recreate and label the relevant training dataset,but such a procedure is time-consuming and labor-intensive.This paper proposed a domain adaptation method that can transfer an existing model trained from one domain to a new domain without extra manual labeling.The method includes three main steps:transform the source fruit image(with labeled information)into the target fruit image(without labeled information)through the CycleGAN network;Automatically label the target fruit image by a pseudo-label process;Improve the labeling accuracy by a pseudo-label self-learning approach.Use a labeled orange image dataset as the source domain,unlabeled apple and tomato image dataset as the target domain,the performance of the proposed method from the perspective of fruit detection has been evaluated.Without manual labeling for target domain image,the mean average precision reached 87.5%for apple detection and 76.9%for tomato detection,which shows that the proposed method can potentially fill the species gap in deep learning-based fruit detection.展开更多
Objective Angiogenesis is known to be essential for the survival,growth,invasion,and metastasis of lung cancer cells. Vascular endothelial growth factor(VEGF) is an important factor regulating angiogenesis of non-smal...Objective Angiogenesis is known to be essential for the survival,growth,invasion,and metastasis of lung cancer cells. Vascular endothelial growth factor(VEGF) is an important factor regulating angiogenesis of non-small cell lung cancer(NSCLC); however,its pathologic features and significance are unclear. In this study,the tissue VEGF expression levels and its gene transcriptional status,as well as circulating VEGF levels,were investigated in patients with lung disease. Methods VEGF protein and m RNA expression levels in 38 lung tissue samples were analyzed by immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR),respectively. Circulating VEGF levels were detected quantitatively by an enzyme linked immuno-sorbent assay. Results The level of VEGF expression was significantly higher in lung cancer tissue than in the corresponding paracancerous or non-cancerous tissues. The average level of VEGF-positive staining was 76% in tissue samples from NSCLC patients; the levels were 89% in tissue samples from stage III patients and 92% in stage IV patients. High VEGF expression was also evident in cases with lymph node metastasis(84%),distant metastasis(90%),and lower differentiation degree(89%). VEGF m RNA in cancerous tissues was represented predominantly by the VEGF121 and VEGF165 isoforms. Circulating VEGF levels were significantly higher in NSCLC patients [(840 ± 324) pg/m L] than in patients with benign lung diseases [(308 ± 96) pg/m L] or in healthy individuals serving as controls [(252 ± 108) pg/m L]. Conclusion The over-expression of lung VEGF and its gene transcription status should be useful molecular indicators for NSCLC diagnosis.展开更多
Background Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally.Both genetic and environmental factors contribute to the illness.If left undiagnosed and untreated,schizophr...Background Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally.Both genetic and environmental factors contribute to the illness.If left undiagnosed and untreated,schizophrenia results in impaired social function,repeated hospital admissions,reduced quality of life and decreased life expectancy.Clinical diagnosis largely relies on subjective evidence,including self-reported experiences,and reported behavioural abnormalities followed by psychiatric evaluation.In addition,psychoses may occur along with other conditions,and the symptoms are often episodic and transient,posing a significant challenge to the precision of diagnosis.Therefore,objective,specific tests using biomarkers are urgently needed for differential diagnosis of schizophrenia in clinical practice.Aims We aimed to provide evidence-based and consensus-based recommendations,with a summary of laboratory measurements that could potentially be used as biomarkers for schizophrenia,and to discuss directions for future research.Methods We searched publications within the last 10 years with the following keywords:‘schizophrenia’,‘gene’,‘inflammation’,‘neurotransmitter’,‘protein marker’,‘gut microbiota’,‘pharmacogenomics’and‘biomarker’.A draft of the consensus was discussed and agreed on by all authors at a round table session.Results We summarised the characteristics of candidate diagnostic markers for schizophrenia,including genetic,inflammatory,neurotransmitter,peripheral protein,pharmacogenomic and gut microbiota markers.We also proposed a novel laboratory process for diagnosing schizophrenia in clinical practice based on the evidence summarised in this paper.Conclusions Further efforts are needed to identify schizophrenia-specific genetic and epigenetic markers for precise diagnosis,differential diagnosis and ethnicity-specific markers for the Chinese population.The development of novel laboratory techniques is making it possible to use these biomarkers clinically to diagnose disease.展开更多
The quantitative evaluation on land use /cover change as well as its influence on landscape pattern under the background of returning grain plots to forestry is significant to the sustainable utilization of land resou...The quantitative evaluation on land use /cover change as well as its influence on landscape pattern under the background of returning grain plots to forestry is significant to the sustainable utilization of land resources and ecological environment reconstruction in the southern Ningxia.Based on multi-temporal remote sensing data from four periods of Landsat TM /ETM,and combination of ecological quantity analytical method with GIS,the change of land use /cover and landscape pattern in Pengyang County of Ningxia Province were analyzed.The conclusions showed that the amount of each land use type was changed with different degrees,the area of forest /grass land increased,while farmland and unused land decreased.The change of landscape pattern was characterized as that the degree of landscape fragmentation,mixed distribution of patches,diversity index and evenness index increased gradually and then decreased,the connectivity between patches decreased gradually and then increased,and landscape shape presented irregular.展开更多
With four ecological migration zones in Huamachi Town of Yanchi County in Ningxia Autonomous Region as the object of study,we carry out the evaluation of ecological benefits in ecological migration zone. Using the SPO...With four ecological migration zones in Huamachi Town of Yanchi County in Ningxia Autonomous Region as the object of study,we carry out the evaluation of ecological benefits in ecological migration zone. Using the SPOT satellite remote sensing image in 2008 and UAV aerophotographic image in 2013,we first monitor and analyze the land use change over five years in the study area,and then adopt ecological green equivalent evaluation model for the evaluation of ecological benefits in the ecological migration zone. Studies have shown that:( i) from 2008 to 2013,the ecological green equivalent in the study area was increased and the ecological environment was improved;( ii) the ecological green equivalent in the study area was less than 1 in 2008 and 2013,and ecological environment was still fragile in the migration zone;( iii)the forest coverage rate of the study area was 20% less than the minimum forest coverage rate of the United Nations,but 15% higher than the forest coverage rate of the Ministry of Environmental Protection. There is a large gap between the forest coverage rate based on ecological green equivalent and optimal forest coverage rate,suggesting that the land use still needs to be adjusted in study area,and it is necessary to increase efforts to strengthen ecological restoration and continue to implement forest conservation,returning land for farming to forestry and other measures.展开更多
Since trastuzumab monotherapy for treatment of breast cancer with HER2/ErbB2 over-expression has been shown to have limited efficacy, combined treatment of trastuzumab with chemotherapy is widely practiced in clinic. ...Since trastuzumab monotherapy for treatment of breast cancer with HER2/ErbB2 over-expression has been shown to have limited efficacy, combined treatment of trastuzumab with chemotherapy is widely practiced in clinic. However, certain combination treatments of trastuzumab and chemotherapy (i.e. doxorubicin) are not recommended due to high risk of cardiotoxicity. Antibody-drug conjugates (ADCs) offer selective delivery of cytotoxic agents into targeted cancer cells, thereby allowing for reduced general cellular cytotoxicity caused by chemotherapeutic agents through antibody mediated specific recognition of tumor antigens. In this study, we constructed a trastuzumab-doxorubicin conjugate (T-Dox) using a thioether linkage and characterized both biophysical stability and anti-cancer potency of the T-Dox using a panel of HER2 expressing cancer cell lines. The T-Dox conjugate showed significantly improved anti-cancer potency in comparison with trastuzumab. The results demonstrated for the first time that there were significant differences in the uptake of T-Dox among high HER2 expression cancer cells and higher T-Dox uptake also showed stronger anti-cancer potency. Similar to trastuzumab, T-Dox selectively bound to HER2 overexpressing cancer cells and low HER2 expression cells had no detectable uptake of T-Dox. Consistent to the uptake data, human cardiomyocyte cells had no detectable HER2 expression and T-Dox showed minimal cytotoxic effects. On the contrary, a treatment with combination of trastuzumab and doxorubicin showed severe cytotoxicity to human cardiomyocytes (>90% cell death after 3 day exposure). This study demonstrated that trastuzumab conjugated with doxorubicin (T-Dox) can provide valuable alternative to the combination treatment with doxorubicin and trastuzumab for high HER2 expressing cancer patients.展开更多
The Efficient Markets Hypothesis(EMH)is the focusing topic in the past 50 years of financial market researches.Many empirical studies are then provided that want to test EMH but have no consensus.The perception of EMH...The Efficient Markets Hypothesis(EMH)is the focusing topic in the past 50 years of financial market researches.Many empirical studies are then provided that want to test EMH but have no consensus.The perception of EMH determines the attitude and strategy of participants and regulators in financial market.One perception of EMH argues that investors’behavior of seeking abnormal profits and arbitrage drives prices to their“correct”value.Investigating the“correct”value derives the concept of“market indeterminacy”.It means the inability to determine whether stock prices are efficient or inefficient.Market indeterminacy pervades stock markets because“correct”prices are unknown because of imperfect information and model sensitivity.Market indeterminacy makes arbitrage risky and makes event studies unreliable in some policy and litigation applications.The concept of market efficiency is needed to be re-recognized considering the mechanism of price formation.In order to further research and practice in law and financial market,there needs a view from the“jumping together”of disparate disciplines.Adaptive Markets Hypothesis(AMH)that using the evolutionary principles in financial market is a new viewpoint on cognitive decision and deserves to be paid more attention to.展开更多
文摘This article examines the soft soil roadbed reinforcement technology for widened sections of highways in a specific project.It provides an overview of the project,the principles of soft soil roadbed reinforcement technology for wide sections,and its practical application.The analysis aims to offer guidance on applying soft soil roadbed wide section reinforcement technology and enhancing the overall quality of similar projects.
文摘In this paper,the geological condition of the right-side slope of the K114+694–K115+162 section of Yong-tai-wen Expressway is investigated and analyzed with the results showing that the strength of rock mass is the main contributor to the stability of the slope.Then,two widening schemes are proposed,which are the steep slope with strong support and the gentle slope with general support schemes.The static/slope module of MIDAS GTS finite element analysis software and the strength reduction method were used to compare the two schemes.The results show that the steep slope with a strong support scheme has obvious advantages in land requisition,environmental protection,and safety and is more suitable for reconstructing and expanding the highway slope.
文摘Objectives:To assess whether the extension of the flushing interval will increase risks of complications associated with totally implantable venous access port(TIVAP)in the off-treatment period.Methods:A retrospective single-center observational study was performed.Patients with a TIVAP in the off-treatment period that underwent regular flushing in our clinic were included.Data concerning patients and their TIVAPs were recorded.Patient baseline characteristics and TIVAP-related complications were analyzed.Continuous variables were analyzed by ANOVA or the Kruskal-Wallis H test.To compare the occurrence of TIVAP-related complications,the chi-square test was used;if needed,Fisher’s exact test was used.Results:Totally 607 patients were reviewed,and 563 patients were finally included.Thirteen complications were recorded,including 11 cases of catheter occlusion(1.95%),one case of port cannula rotation(0.18%),and one case of catheter tip malposition(0.18%).No device-related infection or venous thrombosis was recorded.Among these patients,the average flushing interval was 35.27±13.09 days.Patients were divided into three groups according to the flushing interval:every 28 days or less(Group 1,n=133);every 29-44 days(Group 2,n=350);and every 45 days or more(Group 3,n=80).No significant difference in catheter-related complications was found among the three groups(P>0.05).Conclusions:In the TIVAP off-treatment period,patients without any history of TIVAP-related complications during approximately one year can attempt to prolong the flushing interval to more than 4 weeks;we further suggest that 5-6 weeks may be an appropriate option for these patients.
基金This study was reviewed and approved by the Ethics Committee of Suzhou Ninth People's Hospital.
文摘BACKGROUND Changes in China's fertility policy have led to a significant increase in older pregnant women.At present,there is a lack of analysis of influencing factors and research on predictive models for postpartum depression(PPD)in older pregnant women.AIM To analysis the influencing factors and the construction of predictive models for PPD in older pregnant women.METHODS By adopting a cross-sectional survey research design,239 older pregnant women(≥35 years old)who underwent obstetric examinations and gave birth at Suzhou Ninth People's Hospital from February 2022 to July 2023 were selected as the research subjects.When postpartum women of advanced maternal age came to the hospital for follow-up 42 d after birth,the Edinburgh PPD Scale(EPDS)was used to assess the presence of PPD symptoms.The women were divided into a PPD group and a no-PPD group.Two sets of data were collected for analysis,and a prediction model was constructed.The performance of the predictive model was evaluated using receiver operating characteristic(ROC)analysis and the Hosmer-Lemeshow goodness-of-fit test.RESULTS On the 42nd day after delivery,51 of 239 older pregnant women were evaluated with the EPDS scale and found to have depressive symptoms.The incidence rate was 21.34%(51/239).There were statistically significant differences between the PPD group and the no-PPD group in terms of education level(P=0.004),family relationships(P=0.001),pregnancy complications(P=0.019),and mother–infant separation after birth(P=0.002).Multivariate logistic regression analysis showed that a high school education and below,poor family relationships,pregnancy complications,and the separation of the mother and baby after birth were influencing factors for PPD in older pregnant women(P<0.05).Based on the influencing factors,the following model equation was developed:Logit(P)=0.729×education level+0.942×family relationship+1.137×pregnancy complications+1.285×separation of the mother and infant after birth-6.671.The area under the ROC curve of this prediction model was 0.873(95%CI:0.821-0.924),the sensitivity was 0.871,and the specificity was 0.815.The deviation between the value predicted by the model and the actual value through the Hosmer-Lemeshow goodness-of-fit test was not statistically significant(χ^(2)=2.749,P=0.638),indicating that the model did not show an overfitting phenomenon.CONCLUSION The risk of PPD among older pregnant women is influenced by educational level,family relationships,pregnancy complications,and the separation of the mother and baby after birth.A prediction model based on these factors can effectively predict the risk of PPD in older pregnant women.
基金Supported by The Society Development of Nantong,HS2012039Jiangsu Health Projects,BL2012053,K201102the Priority Academic Program Development of Jiangsu,and the International S and T Cooperation Program,2013DFA32150 of China
文摘AIM: To investigate the dynamic features of insulin-like growth factor-I receptor (IGF-IR) expression in rat hepatocarcinogenesis, and the relationship between IGF-IR and hepatocytes malignant transformation at mRNA or protein level.METHODS: Hepatoma models were made by inducing with 2-fluorenylacetamide (2-FAA) on male Sprague-Dawley rats. Morphological changes of hepatocytes were observed by pathological Hematoxylin and eosin staining, the dynamic expressions of liver and serum IGF-IR were quantitatively analyzed by an enzyme-linked immunosorbent assay. The distribution of hepatic IGF-IR was located by immunohistochemistry. The fragments of IGF-IR gene were amplified by reverse transcription-polymerase chain reaction, and confirmed by sequencing.RESULTS: Rat hepatocytes after induced by 2-FAA were changed dynamically from granule-like degeneration, precancerous to hepatoma formation with the progressing increasing of hepatic mRNA or IGF-IR expression. The incidences of liver IGF-IR, IGF-IR mRNA, specific IGF-IR concentration (ng/mg wet liver), and serum IGF-IR level (ng/mL) were 0.0%, 0.0%, 0.63 ± 0.17, and 1.33 ± 0.47 in the control; 50.0%, 61.1%, 0.65 ± 0.2, and 1.51 ± 0.46 in the degeneration; 88.9%, 100%, 0.66 ± 0.14, and 1.92 ± 0.29 in the precancerosis; and 100%, 100%, 0.96 ± 0.09, and 2.43 ± 0.57 in the cancerous group, respectively. IGF-IR expression in the cancerous group was significantly higher (P < 0.01) than that in any of other groups at mRNA or protein level. The closely positive IGF-IR relationship was found between livers and sera (r = 0.91, t = 14.222, P < 0.01), respectively.CONCLUSION: IGF-IR expression may participate in rat hepatocarcinogenesis and its abnormality should be an early marker for hepatocytes malignant transformation.
基金Supported by Projects of Jiangsu Elitist Peak in Six Fields,Nos.2013-WSN-078,2013-WSW-011,and 2014-YY-028the QingL an Program of Jiangsu Higher Education,the Youth Science Foundation of Nantong Health Department,No.WQ2014005the International Science and Technology Cooperation Program,No.2013DFA32150
文摘AIM: To interfere with the activation of nuclear factor-κB(NF-κB) with metformin and explore its effect in reversing multidrug resistance(MDR) of hepatocellular carcinoma(HCC) cells.METHODS: Expression of P-glycoprotein(P-gp) and NF-κB in human HepG 2 or HepG 2/adriamycin(ADM) cells treated with pC MV-NF-κB-small interference RNA(siR NA) with or without metformin, was analyzed by Western blot or fluorescence quantitative PCR. Cell viability was tested by CCK-8 assay. Cell cycle and apoptosis were measured by flow cytometry and Annexin-V-PE/7-AnnexinV apoptosis detection double staining assay, respectively. RESULTS: P-gp overexpression in HepG 2 and HepG 2/ADM cells was closely related to mdr1 mR NA(3.310 ± 0.154) and NF-κB mR NA(2.580 ± 0.040) expression. NF-κB gene transcription was inhibited by specific siR NA with significant down-regulation of P-gp and enhanced HCC cell chemosensitivity to doxorubicin. After pretreatment with metformin, Hep G2/ADM cells were sensitized to doxorubicin and P-gp was decreased through the NF-κB signaling pathway. The synergistic effect of metformin and NF-κB siR NA were found in HepG 2/ADM cells with regard to proliferation inhibition, cell cycle arrest and inducing cell apoptosis. CONCLUSION: Metformin via silencing NF-κB signaling could effectively reverse MDR of HCC cells by downregulating MDR1/P-gp expression.
基金This research was financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institution
文摘A proteomic study,using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight,was conducted in apple fruit(cv.‘Golden Delicious’)starting at 10 days prior to harvest through 50 days in storage.Total protein was extracted using a phenol/sodium dodecyl sulfate protocol.More than 400 protein spots were detected in each gel and 55 differentially expressed proteins(p,0.05)were subjected to matrix-assisted laser desorption/ionization time-of-flight/time-of-flight analysis.Fifty-three of these proteins were finally identified using an apple expressed sequence tag database downloaded from Genome Database for Rosaceae and placed into six categories.The categories and the percentage of proteins placed in each category were stress response and defense(49.0%),energy and metabolism(34.0%),fruit ripening and senescence(5.6%),signal transduction(3.8%),cell structure(3.8%)and protein synthesis(3.8%).Proteins involved in several multiple metabolic pathways,including glycolysis,pentose–phosphate pathway,anti-oxidative systems,photosynthesis and cell wall synthesis,were downregulated,especially during the climacteric burst in respiration and during the senescent stages of fruit development.Proteins classified as allergens or involved in cell wall degradation were upregulated during the ripening process.Some protein spots exhibited a mixed pattern(increasing to maximal abundance followed by a decrease),such as 1-aminocyclopropane-1-carboxylate oxidase,L-ascorbate peroxidase and abscisic acid response proteins.The identification of differentially expressed proteins associated with physiological processes identified in the current study provides a baseline of information for understanding the metabolic processes and regulatory mechanisms that occur in climacteric apple fruit during ripening and senescence.
基金Supported by Grants from the Jiangsu Provincial Special Programs of Medical ScienceBL2012053+7 种基金HK201102the Nantong Undertaking and Technological InnovationHS2013007BK2013048 and HS2014078the Priority Academic Program Development of Higher Education Institution of Jiangsu Provincethe National Natural Science FoundationNo.81200634the international S&T Cooperation Program(2013DFA32150)of China
文摘AIM: To investigate small interfering RNA(si RNA)-mediated inhibition of nuclear factor-kappa B(NF-κB) activation and multidrug-resistant(MDR) phenotype formation in human Hep G2 cells. METHODS: Total RNA was extracted from human Hep G2 or LO2 cells. NF-κB/p65 m RNA was amplified by nested reverse transcription polymerase chain reaction and confirmed by sequencing. NF-κB/p65 was analyzed by immunohistochemistry. Specific-si RNA was transfected to Hep G2 cells to knock down NF-κB/p65 expression. The effects on cell proliferation, survival, and apoptosis were assessed, and the level of NF-κB/p65 or P-glycoprotein(P-gp) was quantitatively analyzed by enzyme-linked immunosorbent assay.RESULTS: Hep G2 cells express NF-κB/p65 and express relatively less phosphorylated p65(P-p65) and little P-gp. After treatment of Hep G2 cells with different doses of doxorubicin, the expression of NF-κB/p65, P-p65, and especially P-gp were dose-dependently upregulated. After Hep G2 cells were transfected with NF-κB/p65 si RNA(100 nmol/L), the expression of NF-κB/p65, P-p65, and P-gp were downregulatedsignificantly and dose-dependently. The viability of Hep G2 cells was decreased to 23% in the combination NF-κB/p65 si RNA(100 nmol/L) and doxorubicin(0.5 μmol/L) group and 47% in the doxorubicin(0.5 μmol/L) group(t = 7.043, P < 0.001). CONCLUSION: Knockdown of NF-κB/p65 with si RNA is an effective strategy for inhibiting Hep G2 cell growth by downregulating P-gp expression associated chemosensitization and apoptosis induction.
基金supported by National Natural Science Foundation of China(NSFC)program U19A2061Japan Science and Technology Agency(JST)CREST program JPMJCR1512,SICORP Program JPMJSC16H2 and aXis program JPMJAS2018.
文摘Fruit detection and counting are essential tasks for horticulture research.With computer vision technology development,fruit detection techniques based on deep learning have been widely used in modern orchards.However,most deep learning-based fruit detection models are generated based on fully supervised approaches,which means a model trained with one domain species may not be transferred to another.There is always a need to recreate and label the relevant training dataset,but such a procedure is time-consuming and labor-intensive.This paper proposed a domain adaptation method that can transfer an existing model trained from one domain to a new domain without extra manual labeling.The method includes three main steps:transform the source fruit image(with labeled information)into the target fruit image(without labeled information)through the CycleGAN network;Automatically label the target fruit image by a pseudo-label process;Improve the labeling accuracy by a pseudo-label self-learning approach.Use a labeled orange image dataset as the source domain,unlabeled apple and tomato image dataset as the target domain,the performance of the proposed method from the perspective of fruit detection has been evaluated.Without manual labeling for target domain image,the mean average precision reached 87.5%for apple detection and 76.9%for tomato detection,which shows that the proposed method can potentially fill the species gap in deep learning-based fruit detection.
基金Supported in part by a grant from the Project of Health Department of Jiangsu ProvinceChina(No.H201454)
文摘Objective Angiogenesis is known to be essential for the survival,growth,invasion,and metastasis of lung cancer cells. Vascular endothelial growth factor(VEGF) is an important factor regulating angiogenesis of non-small cell lung cancer(NSCLC); however,its pathologic features and significance are unclear. In this study,the tissue VEGF expression levels and its gene transcriptional status,as well as circulating VEGF levels,were investigated in patients with lung disease. Methods VEGF protein and m RNA expression levels in 38 lung tissue samples were analyzed by immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR),respectively. Circulating VEGF levels were detected quantitatively by an enzyme linked immuno-sorbent assay. Results The level of VEGF expression was significantly higher in lung cancer tissue than in the corresponding paracancerous or non-cancerous tissues. The average level of VEGF-positive staining was 76% in tissue samples from NSCLC patients; the levels were 89% in tissue samples from stage III patients and 92% in stage IV patients. High VEGF expression was also evident in cases with lymph node metastasis(84%),distant metastasis(90%),and lower differentiation degree(89%). VEGF m RNA in cancerous tissues was represented predominantly by the VEGF121 and VEGF165 isoforms. Circulating VEGF levels were significantly higher in NSCLC patients [(840 ± 324) pg/m L] than in patients with benign lung diseases [(308 ± 96) pg/m L] or in healthy individuals serving as controls [(252 ± 108) pg/m L]. Conclusion The over-expression of lung VEGF and its gene transcription status should be useful molecular indicators for NSCLC diagnosis.
基金This study was funded by Shanghai Natural Science fund Project under grant(20ZR1447700)The National Key Development Plan for Precision Medicine Research(2017YFC0910002)+4 种基金the hospital-level project of Shanghai Mental Health Center under grant(2020-YJ05)Special Fund for Clinical Research of Wu Jieping Medical Foundation(320675015232)the Hospital level project of Shanghai Mental Health Center(2016-YJ-09)Chinese medicine research project of Shanghai Health and Family Planning Commission(2018LP024)the characteristic discipline construction project of Shanghai Mental Health Center(2017-TSXK-07),China Public Health Alliance(first batch)(GWLM202014).
文摘Background Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally.Both genetic and environmental factors contribute to the illness.If left undiagnosed and untreated,schizophrenia results in impaired social function,repeated hospital admissions,reduced quality of life and decreased life expectancy.Clinical diagnosis largely relies on subjective evidence,including self-reported experiences,and reported behavioural abnormalities followed by psychiatric evaluation.In addition,psychoses may occur along with other conditions,and the symptoms are often episodic and transient,posing a significant challenge to the precision of diagnosis.Therefore,objective,specific tests using biomarkers are urgently needed for differential diagnosis of schizophrenia in clinical practice.Aims We aimed to provide evidence-based and consensus-based recommendations,with a summary of laboratory measurements that could potentially be used as biomarkers for schizophrenia,and to discuss directions for future research.Methods We searched publications within the last 10 years with the following keywords:‘schizophrenia’,‘gene’,‘inflammation’,‘neurotransmitter’,‘protein marker’,‘gut microbiota’,‘pharmacogenomics’and‘biomarker’.A draft of the consensus was discussed and agreed on by all authors at a round table session.Results We summarised the characteristics of candidate diagnostic markers for schizophrenia,including genetic,inflammatory,neurotransmitter,peripheral protein,pharmacogenomic and gut microbiota markers.We also proposed a novel laboratory process for diagnosing schizophrenia in clinical practice based on the evidence summarised in this paper.Conclusions Further efforts are needed to identify schizophrenia-specific genetic and epigenetic markers for precise diagnosis,differential diagnosis and ethnicity-specific markers for the Chinese population.The development of novel laboratory techniques is making it possible to use these biomarkers clinically to diagnose disease.
基金Supported by National Natural Science Foundation(41161081)
文摘The quantitative evaluation on land use /cover change as well as its influence on landscape pattern under the background of returning grain plots to forestry is significant to the sustainable utilization of land resources and ecological environment reconstruction in the southern Ningxia.Based on multi-temporal remote sensing data from four periods of Landsat TM /ETM,and combination of ecological quantity analytical method with GIS,the change of land use /cover and landscape pattern in Pengyang County of Ningxia Province were analyzed.The conclusions showed that the amount of each land use type was changed with different degrees,the area of forest /grass land increased,while farmland and unused land decreased.The change of landscape pattern was characterized as that the degree of landscape fragmentation,mixed distribution of patches,diversity index and evenness index increased gradually and then decreased,the connectivity between patches decreased gradually and then increased,and landscape shape presented irregular.
基金Supported by National Natural Science Foundation of China(41161081)
文摘With four ecological migration zones in Huamachi Town of Yanchi County in Ningxia Autonomous Region as the object of study,we carry out the evaluation of ecological benefits in ecological migration zone. Using the SPOT satellite remote sensing image in 2008 and UAV aerophotographic image in 2013,we first monitor and analyze the land use change over five years in the study area,and then adopt ecological green equivalent evaluation model for the evaluation of ecological benefits in the ecological migration zone. Studies have shown that:( i) from 2008 to 2013,the ecological green equivalent in the study area was increased and the ecological environment was improved;( ii) the ecological green equivalent in the study area was less than 1 in 2008 and 2013,and ecological environment was still fragile in the migration zone;( iii)the forest coverage rate of the study area was 20% less than the minimum forest coverage rate of the United Nations,but 15% higher than the forest coverage rate of the Ministry of Environmental Protection. There is a large gap between the forest coverage rate based on ecological green equivalent and optimal forest coverage rate,suggesting that the land use still needs to be adjusted in study area,and it is necessary to increase efforts to strengthen ecological restoration and continue to implement forest conservation,returning land for farming to forestry and other measures.
文摘Since trastuzumab monotherapy for treatment of breast cancer with HER2/ErbB2 over-expression has been shown to have limited efficacy, combined treatment of trastuzumab with chemotherapy is widely practiced in clinic. However, certain combination treatments of trastuzumab and chemotherapy (i.e. doxorubicin) are not recommended due to high risk of cardiotoxicity. Antibody-drug conjugates (ADCs) offer selective delivery of cytotoxic agents into targeted cancer cells, thereby allowing for reduced general cellular cytotoxicity caused by chemotherapeutic agents through antibody mediated specific recognition of tumor antigens. In this study, we constructed a trastuzumab-doxorubicin conjugate (T-Dox) using a thioether linkage and characterized both biophysical stability and anti-cancer potency of the T-Dox using a panel of HER2 expressing cancer cell lines. The T-Dox conjugate showed significantly improved anti-cancer potency in comparison with trastuzumab. The results demonstrated for the first time that there were significant differences in the uptake of T-Dox among high HER2 expression cancer cells and higher T-Dox uptake also showed stronger anti-cancer potency. Similar to trastuzumab, T-Dox selectively bound to HER2 overexpressing cancer cells and low HER2 expression cells had no detectable uptake of T-Dox. Consistent to the uptake data, human cardiomyocyte cells had no detectable HER2 expression and T-Dox showed minimal cytotoxic effects. On the contrary, a treatment with combination of trastuzumab and doxorubicin showed severe cytotoxicity to human cardiomyocytes (>90% cell death after 3 day exposure). This study demonstrated that trastuzumab conjugated with doxorubicin (T-Dox) can provide valuable alternative to the combination treatment with doxorubicin and trastuzumab for high HER2 expressing cancer patients.
文摘The Efficient Markets Hypothesis(EMH)is the focusing topic in the past 50 years of financial market researches.Many empirical studies are then provided that want to test EMH but have no consensus.The perception of EMH determines the attitude and strategy of participants and regulators in financial market.One perception of EMH argues that investors’behavior of seeking abnormal profits and arbitrage drives prices to their“correct”value.Investigating the“correct”value derives the concept of“market indeterminacy”.It means the inability to determine whether stock prices are efficient or inefficient.Market indeterminacy pervades stock markets because“correct”prices are unknown because of imperfect information and model sensitivity.Market indeterminacy makes arbitrage risky and makes event studies unreliable in some policy and litigation applications.The concept of market efficiency is needed to be re-recognized considering the mechanism of price formation.In order to further research and practice in law and financial market,there needs a view from the“jumping together”of disparate disciplines.Adaptive Markets Hypothesis(AMH)that using the evolutionary principles in financial market is a new viewpoint on cognitive decision and deserves to be paid more attention to.
