The role of B7-1 in podocyte injury has received increasing attention.The aim of this study was to investigate whether losartan protects podocytes of patients with diabetic kidney disease(DKD)by regulating B7-1 and th...The role of B7-1 in podocyte injury has received increasing attention.The aim of this study was to investigate whether losartan protects podocytes of patients with diabetic kidney disease(DKD)by regulating B7-1 and the underlying mechanisms.Rats with streptozotocin-induced DKD were treated with losartan for 8 weeks.Biochemical changes in blood and urine were analyzed.Kidneys were isolated for electron microscopy,immunofluorescence,real-time quantitative PCR(RT-PCR),and Western blot analysis.Immortalized mouse podocyte cells were cultured in normal or high glucose medium in the presence or absence of losartan for 48 h,and then the cells were collected for immunofluorescence,PCR,Western blotting and monolayer permeability detection.The phosphatidylinositol 3-kinase(PI3K)110a subunit and angiotensin II type 1 receptor(AT1R)plasmids were transfected into podocytes,respectively,and then Western blotting was performed to assess the expression of B7-1 protein.The results showed that losartan ameliorated podocyte structure and function in the rat model of DKD,and reduced the expression of B7-1 protein.Overexpression of PI3K 110a subunit in podocytes attenuated the inhibitory effect of losartan on B7-1 expression in high glucose-stimulated podocytes.The expression of B7-1 was significantly increased by overexpression of ATI R and significantly reduced by blocking PI3K 110a subunit.We conclude that losartan protects podocytes against high glucose-induced injury by inhibiting AT1R-mediated B7-1 expression.This effect is dependent on the AT1R-PI3K 110a subunit pathway.展开更多
本文报道了将小尺寸二硫化锡(SnS_(2))纳米颗粒嵌入到二维多孔碳纳米片(PCN)中间层,形成多功能纳米复合材料(PCN-SnS_(2))作为硫正极载体,从而降低穿梭效应,实现锂硫电池快充.一方面,复合材料中石墨化碳纳米片可整体性提高电极的导电性...本文报道了将小尺寸二硫化锡(SnS_(2))纳米颗粒嵌入到二维多孔碳纳米片(PCN)中间层,形成多功能纳米复合材料(PCN-SnS_(2))作为硫正极载体,从而降低穿梭效应,实现锂硫电池快充.一方面,复合材料中石墨化碳纳米片可整体性提高电极的导电性.另一方面,复合材料丰富的孔道既促进离子转移和电解质的渗透,又缓解充放电过程中的体积变化,从而确保电极材料的完整性.特别地,PCN的物理限域和小尺寸SnS_(2)纳米颗粒的强化学吸附协同作用可有效降低多硫化物的穿梭效应.因此,PCN-SnS_(2)-S电极具有良好的电化学性能,即使在2 C的高电流密度下,150圈循环后仍可维持650 mA h g^(-1)的放电容量.本研究工作为小尺寸SnS_(2)纳米结构更利于捕获多硫化物以减弱锂硫电池的穿梭效应提供了理论基础.展开更多
基金the National Natural Science Foundation of China(No.81400333).
文摘The role of B7-1 in podocyte injury has received increasing attention.The aim of this study was to investigate whether losartan protects podocytes of patients with diabetic kidney disease(DKD)by regulating B7-1 and the underlying mechanisms.Rats with streptozotocin-induced DKD were treated with losartan for 8 weeks.Biochemical changes in blood and urine were analyzed.Kidneys were isolated for electron microscopy,immunofluorescence,real-time quantitative PCR(RT-PCR),and Western blot analysis.Immortalized mouse podocyte cells were cultured in normal or high glucose medium in the presence or absence of losartan for 48 h,and then the cells were collected for immunofluorescence,PCR,Western blotting and monolayer permeability detection.The phosphatidylinositol 3-kinase(PI3K)110a subunit and angiotensin II type 1 receptor(AT1R)plasmids were transfected into podocytes,respectively,and then Western blotting was performed to assess the expression of B7-1 protein.The results showed that losartan ameliorated podocyte structure and function in the rat model of DKD,and reduced the expression of B7-1 protein.Overexpression of PI3K 110a subunit in podocytes attenuated the inhibitory effect of losartan on B7-1 expression in high glucose-stimulated podocytes.The expression of B7-1 was significantly increased by overexpression of ATI R and significantly reduced by blocking PI3K 110a subunit.We conclude that losartan protects podocytes against high glucose-induced injury by inhibiting AT1R-mediated B7-1 expression.This effect is dependent on the AT1R-PI3K 110a subunit pathway.
基金the National Key R&D Program of China(2016YFA0202602)the National Natural Science Foundation of China(U1663225)+3 种基金the Fundamental Research Funds for the Central Universities(2020-YB-009)the Academy of Scientific Research and Technology(6611,ASRT,Egypt)the 111 National project(B20002)from the Ministry of Science and Technology and the Ministry of Education,ChinaSinopec Ministry of Science and Technology Basic Prospective Research Project(217027-5 and 218025-9)。
文摘本文报道了将小尺寸二硫化锡(SnS_(2))纳米颗粒嵌入到二维多孔碳纳米片(PCN)中间层,形成多功能纳米复合材料(PCN-SnS_(2))作为硫正极载体,从而降低穿梭效应,实现锂硫电池快充.一方面,复合材料中石墨化碳纳米片可整体性提高电极的导电性.另一方面,复合材料丰富的孔道既促进离子转移和电解质的渗透,又缓解充放电过程中的体积变化,从而确保电极材料的完整性.特别地,PCN的物理限域和小尺寸SnS_(2)纳米颗粒的强化学吸附协同作用可有效降低多硫化物的穿梭效应.因此,PCN-SnS_(2)-S电极具有良好的电化学性能,即使在2 C的高电流密度下,150圈循环后仍可维持650 mA h g^(-1)的放电容量.本研究工作为小尺寸SnS_(2)纳米结构更利于捕获多硫化物以减弱锂硫电池的穿梭效应提供了理论基础.
