Light quality and intensity can have a significant impact on plant health and crop productivity.Chlorophylls and carotenoids are classes of plant pigments that are responsible for harvesting light energy and protectin...Light quality and intensity can have a significant impact on plant health and crop productivity.Chlorophylls and carotenoids are classes of plant pigments that are responsible for harvesting light energy and protecting plants from the damaging effects of intense light.Our understanding of the role played by plant pigments in light sensitivity has been aided by light-sensitive mutants that change colors upon exposure to light of variable intensity.In this study,we conducted transcriptomic,metabolomic,and hormone analyses on a novel yellowing mutant of pepper(yl1)to shed light on the molecular mechanism that regulates the transition from green to yellow leaves in this mutant upon exposure to high-intensity light.Our results revealed greater accumulation of the carotenoid precursor phytoene and the carotenoids phytofluene,antheraxanthin,and zeaxanthin in yl1 compared with wild-type plants under high light intensity.A transcriptomic analysis confirmed that enzymes involved in zeaxanthin and antheraxanthin biosynthesis were upregulated in yl1 upon exposure to high-intensity light.We also identified a single basic helix–loop–helix(bHLH)transcription factor,bHLH71-like,that was differentially expressed and positively correlated with light intensity in yl1.Silencing of bHLH71-like in pepper plants suppressed the yellowing phenotype and led to reduced accumulation of zeaxanthin and antheraxanthin.We propose that the yellow phenotype of yl1 induced by high light intensity could be caused by an increase in yellow carotenoid pigments,concurrent with a decrease in chlorophyll accumulation.Our results also suggest that bHLH71-like functions as a positive regulator of carotenoid biosynthesis in pepper.展开更多
Light is an important environmental factor for plant growth and development,different light qualities have different regulatory effects on plants.To investigate the effect of light quality on plants,we determined the ...Light is an important environmental factor for plant growth and development,different light qualities have different regulatory effects on plants.To investigate the effect of light quality on plants,we determined the physiological characteristics,transcriptome and metabolome analysis of pepper yellowing mutants yl1 treated with blue,red,green,and purple light.Results showed that the leaf of yl1 was obviously yellowing,and the contents of chlorophyll,carotenoid and net photosynthetic rate in yl1 were significantly decreased under purple light.A total of 31,853 genes were quantified under blue,red,green and purple light.The genes related to carotenoid metabolism pathway such as PSY,LUT5 and VDE were significantly increased,while the expression levels of chlorophyll synthesis related genes POR and CAO were significantly decreased under purple light.At the same time,21 carotenoid pathway metabolites were detected under the four light different lights,and 10 metabolites were more abundant in pepper leaves.α-carotene,β-carotene,lutein,neoxanthin,α-cryptoxanthin andβ-cryptoxanthin were significantly accumulated under blue,red and green light.However,zeaxanthin and antheraxanthin were accumulated in large quantities under purple light.After silencing the CaVDE gene under purple light,leaf etiolation degree was significantly weakened,chlorophyll,carotenoids and net photosynthetic rate were significantly increased,and the accumulation of zeaxanthin and antheraxanthin was significantly decreased.These results provide a reference for analyzing the changes of carotenoid components induced by VDE in purple light and provide new insights into the mechanism of leaf color change in plants.展开更多
基金This research was funded by the Special Project of Biological Seed Industry and Fine and Deep Processing of Agricultural Products(grant 202202AE090031)the Project of Education Department of Hunan Province(grant 22B0229)the Key Research and Development Program of Hainan Province(grant ZD2020060).
文摘Light quality and intensity can have a significant impact on plant health and crop productivity.Chlorophylls and carotenoids are classes of plant pigments that are responsible for harvesting light energy and protecting plants from the damaging effects of intense light.Our understanding of the role played by plant pigments in light sensitivity has been aided by light-sensitive mutants that change colors upon exposure to light of variable intensity.In this study,we conducted transcriptomic,metabolomic,and hormone analyses on a novel yellowing mutant of pepper(yl1)to shed light on the molecular mechanism that regulates the transition from green to yellow leaves in this mutant upon exposure to high-intensity light.Our results revealed greater accumulation of the carotenoid precursor phytoene and the carotenoids phytofluene,antheraxanthin,and zeaxanthin in yl1 compared with wild-type plants under high light intensity.A transcriptomic analysis confirmed that enzymes involved in zeaxanthin and antheraxanthin biosynthesis were upregulated in yl1 upon exposure to high-intensity light.We also identified a single basic helix–loop–helix(bHLH)transcription factor,bHLH71-like,that was differentially expressed and positively correlated with light intensity in yl1.Silencing of bHLH71-like in pepper plants suppressed the yellowing phenotype and led to reduced accumulation of zeaxanthin and antheraxanthin.We propose that the yellow phenotype of yl1 induced by high light intensity could be caused by an increase in yellow carotenoid pigments,concurrent with a decrease in chlorophyll accumulation.Our results also suggest that bHLH71-like functions as a positive regulator of carotenoid biosynthesis in pepper.
基金Supported by the Project of Education Department of Hunan Province (Grant No.22B0229)the China Agriculture Research System of MOF and MARA (Grant No.CARS-24-A05)the Special Project of Biological Seed Industry and Fine and Deep Processing of Agricultural Products (Grant No.202202AE090031)。
文摘Light is an important environmental factor for plant growth and development,different light qualities have different regulatory effects on plants.To investigate the effect of light quality on plants,we determined the physiological characteristics,transcriptome and metabolome analysis of pepper yellowing mutants yl1 treated with blue,red,green,and purple light.Results showed that the leaf of yl1 was obviously yellowing,and the contents of chlorophyll,carotenoid and net photosynthetic rate in yl1 were significantly decreased under purple light.A total of 31,853 genes were quantified under blue,red,green and purple light.The genes related to carotenoid metabolism pathway such as PSY,LUT5 and VDE were significantly increased,while the expression levels of chlorophyll synthesis related genes POR and CAO were significantly decreased under purple light.At the same time,21 carotenoid pathway metabolites were detected under the four light different lights,and 10 metabolites were more abundant in pepper leaves.α-carotene,β-carotene,lutein,neoxanthin,α-cryptoxanthin andβ-cryptoxanthin were significantly accumulated under blue,red and green light.However,zeaxanthin and antheraxanthin were accumulated in large quantities under purple light.After silencing the CaVDE gene under purple light,leaf etiolation degree was significantly weakened,chlorophyll,carotenoids and net photosynthetic rate were significantly increased,and the accumulation of zeaxanthin and antheraxanthin was significantly decreased.These results provide a reference for analyzing the changes of carotenoid components induced by VDE in purple light and provide new insights into the mechanism of leaf color change in plants.
