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Apoptotic Effect of Cryptotanshinone on Human Melanoma A375 Cells
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作者 Jinqian LI Jiaru WANG +9 位作者 Lingqi MENG Yi ZHANG Hao WANG Wanting XU Yang LIU Yu ZHANG Shinong WANG yuqing zhai Yinghua LUO Chenghao JIN 《Medicinal Plant》 CAS 2018年第6期39-42,共4页
[Objectives] The aim was to investigate the effect of cryptotanshinone on apoptosis of human melanoma A375 cells and its related mechanism of mitochondrial pathway.[Methods]The cytotoxic effect of cryptotanshinone on ... [Objectives] The aim was to investigate the effect of cryptotanshinone on apoptosis of human melanoma A375 cells and its related mechanism of mitochondrial pathway.[Methods]The cytotoxic effect of cryptotanshinone on apoptosis of human melanoma A375 cells was detected by MTT colorimetry.The apoptosis of melanoma A375 cells was detected with Annexin V-FITC/PI and observed by fluorescence inverted microscope.The expression of apoptosis-related proteins was detected by Western blotting.[Results]The viability of the A375 cells decreased with the increase of drug concentration.The fluorescence intensity of the cells increased with the treatment time.The expression of pro-apoptotic protein caspase-3 gradually increased,while the expression of apoptosis-inhibiting proteins p-AKT and Bcl-2 gradually reduced.[Conclusions]Cryptotanshinone induces apoptosis of human melanoma A375 cells via AKT signaling pathway,thus exerting a good cytotoxic effect on A375 cells. 展开更多
关键词 CRYPTOTANSHINONE Human MELANOMA A375 cells CYTOTOXIC EFFECT Apoptosis AKT signaling pathway
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Advance in Research on Anti-tumor Pharmacological Mechanism of Cryptotanshinone
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作者 Jiaru WANG Lingqi MENG +9 位作者 Yi ZHANG Hao WANG Wanting XU Yang LIU Yu ZHANG Tong ZHANG Shinong WANG yuqing zhai Yinghua LUO Chenghao JIN 《Medicinal Plant》 CAS 2018年第6期11-14,共4页
Cryptotanshinone is a natural active ingredient extracted from traditional Chinese medicine Radix Salviae Miltiorrhizae.It has a variety of pharmacological effects,in addition to preventing and treating ischemic disea... Cryptotanshinone is a natural active ingredient extracted from traditional Chinese medicine Radix Salviae Miltiorrhizae.It has a variety of pharmacological effects,in addition to preventing and treating ischemic diseases,coronary artery disease Alzheimer's disease and fighting tumors.The anti-tumor effects are exerted through inhibiting tumor cell proliferation,inducing tumor cell apoptosis,inhibiting tumor metastasis and invasion,inhibiting angiogenesis and regulating reactive oxygen species levels.The studies on the anti-tumor activity and action mechanism of cryptotanshinone in recent years are reviewed in this article. 展开更多
关键词 CRYPTOTANSHINONE ANTI-TUMOR CELL PROLIFERATION APOPTOSIS
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Target Extension-Activated DNA Walker on Nanoparticles for Digital Counting-Based Analysis of MicroRNA 被引量:1
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作者 Qinya Feng yuqing zhai +1 位作者 Wei Ren Chenghui Liu 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2021年第6期1471-1476,共6页
MicroRNAs(miRNAs),especially exosomal miRNAs,are promising noninvasive biomarkers in early-stage cancer diagnosis and disease treatment monitoring.However,their precise and sensitive quantification remains challenging... MicroRNAs(miRNAs),especially exosomal miRNAs,are promising noninvasive biomarkers in early-stage cancer diagnosis and disease treatment monitoring.However,their precise and sensitive quantification remains challenging due to their small size and low abundance.Herein,we have developed a nanoparticle-confined DNA walker strategy for the specific detection of miRNA.In the existence of the target miRNA;the on-particle DNA walking reaction will be initiated,providing a fluorescence-positive nanoparticle.Otherwise,the nanoparticle would be fluorescence-negative.Utilizing the total internal reflection fluorescent microscope(TIRFM)to digitally count the fluorescence-positive nanoparticles,the proposed method possesses a detection limit of 0.2 pmol/L miRNA and can accurately distinguish the single-base mismatched target.This design combines the merits of the DNA walker for signal amplification and the TIRFM for highly sensitive detection,paving a new way for the digital counting-based analysis of exosomal miRNAs. 展开更多
关键词 RNA recognition DNA cleavage Digital counting Total internal reflection fluorescent microscope NANOPARTICLES
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