NAC(NAM/ATAF1/2/CUC2)transcription factors are central switches of growth and stress responses in plants.However,unpredictable interspecies conservation of function and regulatory targets makes the well-studied NAC or...NAC(NAM/ATAF1/2/CUC2)transcription factors are central switches of growth and stress responses in plants.However,unpredictable interspecies conservation of function and regulatory targets makes the well-studied NAC orthologs inapt for pulse engineering.The knowledge of suitable NAC candidates in hardy pulses like cowpea(Vigna unguiculata(L.)Walp.)is still in infancy,hence warrants immediate biotechnological intervention.Here,we showed that overexpression of two native NAC genes(VuNAC1and VuNAC2)promoted germinative,vegetative,and reproductive growth and conferred multiple abiotic stress tolerance in a commercial cowpea variety.The transgenic lines displayed increased leaf area,thicker stem,nodule-rich denser root system,early flowering,higher pod production(~3.2-fold and~2.1-fold),and greater seed weight(10.3%and 6.0%).In contrast,transient suppression of VuNAC1/2 caused severe growth retardation and flower inhibition.The overexpressor lines showed remarkable tolerance to major yielddeclining terminal stresses,such as drought,salinity,heat,and cold,and recovered growth and seed production by boosting photosynthetic activity,water use efficiency,membrane integrity,Na^(+)/K^(+)homeostasis,and antioxidant activity.The comparative transcriptome study indicated consolidated activation of genes involved in chloroplast development,photosynthetic complexes,cell division and expansion,cell wall biogenesis,nutrient uptake and metabolism,stress response,abscisic acid,and auxin signaling.Unlike their orthologs,VuNAC1/2 direct synergistic transcriptional tuning of stress and developmental signaling to avoid unwanted trade-offs.Their overexpression governs the favorable interplay of photosynthesis and reactive oxygen species regulation to improve stress recovery,nutritional sufficiency,biomass,and production.This unconventional balance of strong stress tolerance and agronomic quality is useful for translational crop research and molecular breeding of pulses.展开更多
Hydrogen peroxide （H202） is a reactive oxygen species that affects cell signaling in various plant defense responses and induces programmed cell death. To identify the new components associated with H202 signaling a...Hydrogen peroxide （H202） is a reactive oxygen species that affects cell signaling in various plant defense responses and induces programmed cell death. To identify the new components associated with H202 signaling and tolerance, we conducted a genome-wide association study （GWAS） on the root growth of 133 Arabidopsis thaliana accessions grown in the presence of toxic H2O2 levels. The most significant SNPs were associated with a cluster of chromosome 4 genes encoding an aquaporin NODULIN 26-LIKE INTRINSIC PROTEIN 1; 1 （NIP1;1）, an NB-ARC domain-containing disease resistance protein （AT4G19050）, and a putative membrane lipoprotein （AT4G19070）. The expression level of NIP1;1 was relatively high in A. thaliana accessions sensitive to H2O2. Additionally, overexpression of NIP1;1 in a tolerant accession （e.g., Col-0） increased the sensitivity of transgenic plants to H2O2. An in planta β-glucuronidase reporter assay revealed that variations in the NIP1;1 promoter were responsible for the differences of its expression level in H2O2-tolerant and -sensitive accessions. Cell death was extensive and H2O2 levels were high in the roots of H2O2-sensitive and NIP1;1-overexpressing accessions. Together, our results indicate that the aquaporin NIP1;1 is a key determinant of the sensitivity ofA. thaliana to H2O2, and contributes to the phenotypic variations detected by our GWAS.展开更多
Transcriptional regulation plays a crucial role in plant adaptation to diverse environments.Several transcription factors(TFs),the so-called master switch TFs or hub TFs,regulate various genes critical for adaptation ...Transcriptional regulation plays a crucial role in plant adaptation to diverse environments.Several transcription factors(TFs),the so-called master switch TFs or hub TFs,regulate various genes critical for adaptation to different stresses.STOP1(SENSITIVE TO PROTON RHIZOTOXICITY 1),a zinc-finger TF of Arabidopsis(Arabidopsis thaliana),is one such master/hub TF that transcriptionally regulates multiple stress tolerance(Sadhukhan et al.,2021).STOP1 plays a critical role in tolerance to acid soil syndrome(i.e.,H+and Al3+tolerance)(Iuchi et al.,2007)and hypoxia tolerance(Enomoto et al.,2019),and negatively regulates drought tolerance(Sadhukhan et al.,2019).In addition,Tian et al.展开更多
基金supported by a research grant from the Program Support Grant Phase-II from the Department of Biotechnology,Government of India to L.S. (BT/PR13560/COE/34/44/2015)。
文摘NAC(NAM/ATAF1/2/CUC2)transcription factors are central switches of growth and stress responses in plants.However,unpredictable interspecies conservation of function and regulatory targets makes the well-studied NAC orthologs inapt for pulse engineering.The knowledge of suitable NAC candidates in hardy pulses like cowpea(Vigna unguiculata(L.)Walp.)is still in infancy,hence warrants immediate biotechnological intervention.Here,we showed that overexpression of two native NAC genes(VuNAC1and VuNAC2)promoted germinative,vegetative,and reproductive growth and conferred multiple abiotic stress tolerance in a commercial cowpea variety.The transgenic lines displayed increased leaf area,thicker stem,nodule-rich denser root system,early flowering,higher pod production(~3.2-fold and~2.1-fold),and greater seed weight(10.3%and 6.0%).In contrast,transient suppression of VuNAC1/2 caused severe growth retardation and flower inhibition.The overexpressor lines showed remarkable tolerance to major yielddeclining terminal stresses,such as drought,salinity,heat,and cold,and recovered growth and seed production by boosting photosynthetic activity,water use efficiency,membrane integrity,Na^(+)/K^(+)homeostasis,and antioxidant activity.The comparative transcriptome study indicated consolidated activation of genes involved in chloroplast development,photosynthetic complexes,cell division and expansion,cell wall biogenesis,nutrient uptake and metabolism,stress response,abscisic acid,and auxin signaling.Unlike their orthologs,VuNAC1/2 direct synergistic transcriptional tuning of stress and developmental signaling to avoid unwanted trade-offs.Their overexpression governs the favorable interplay of photosynthesis and reactive oxygen species regulation to improve stress recovery,nutritional sufficiency,biomass,and production.This unconventional balance of strong stress tolerance and agronomic quality is useful for translational crop research and molecular breeding of pulses.
文摘Hydrogen peroxide （H202） is a reactive oxygen species that affects cell signaling in various plant defense responses and induces programmed cell death. To identify the new components associated with H202 signaling and tolerance, we conducted a genome-wide association study （GWAS） on the root growth of 133 Arabidopsis thaliana accessions grown in the presence of toxic H2O2 levels. The most significant SNPs were associated with a cluster of chromosome 4 genes encoding an aquaporin NODULIN 26-LIKE INTRINSIC PROTEIN 1; 1 （NIP1;1）, an NB-ARC domain-containing disease resistance protein （AT4G19050）, and a putative membrane lipoprotein （AT4G19070）. The expression level of NIP1;1 was relatively high in A. thaliana accessions sensitive to H2O2. Additionally, overexpression of NIP1;1 in a tolerant accession （e.g., Col-0） increased the sensitivity of transgenic plants to H2O2. An in planta β-glucuronidase reporter assay revealed that variations in the NIP1;1 promoter were responsible for the differences of its expression level in H2O2-tolerant and -sensitive accessions. Cell death was extensive and H2O2 levels were high in the roots of H2O2-sensitive and NIP1;1-overexpressing accessions. Together, our results indicate that the aquaporin NIP1;1 is a key determinant of the sensitivity ofA. thaliana to H2O2, and contributes to the phenotypic variations detected by our GWAS.
基金H.K.was supported by the Japan Society for the Promotion of Science,KAKENHI(21H02088).
文摘Transcriptional regulation plays a crucial role in plant adaptation to diverse environments.Several transcription factors(TFs),the so-called master switch TFs or hub TFs,regulate various genes critical for adaptation to different stresses.STOP1(SENSITIVE TO PROTON RHIZOTOXICITY 1),a zinc-finger TF of Arabidopsis(Arabidopsis thaliana),is one such master/hub TF that transcriptionally regulates multiple stress tolerance(Sadhukhan et al.,2021).STOP1 plays a critical role in tolerance to acid soil syndrome(i.e.,H+and Al3+tolerance)(Iuchi et al.,2007)and hypoxia tolerance(Enomoto et al.,2019),and negatively regulates drought tolerance(Sadhukhan et al.,2019).In addition,Tian et al.
