Biomaterials with suitable osteoimmunomodulation properties and ability to deliver osteoinductive biomolecules,such as bone morphogenetic proteins,are desired for bone regeneration.Herein,we report the development of ...Biomaterials with suitable osteoimmunomodulation properties and ability to deliver osteoinductive biomolecules,such as bone morphogenetic proteins,are desired for bone regeneration.Herein,we report the development of mesoporous silica rods with large cone-shaped pores(MSR-CP)to load and deliver large protein drugs.It is noted that those cone-shaped pores on the surface modulated the immune response and reduced the pro-inflammatory reaction of stimulated macrophage.Furthermore,bone morphogenetic proteins 2(BMP-2)loaded MSR-CP facilitated osteogenic differentiation and promoted osteogenesis of bone marrow stromal cells.In vivo tests confirmed BMP-2 loaded MSR-CP improved the bone regeneration performance.This study provides a potential strategy for the design of drug delivery systems for bone regeneration.展开更多
The real-time monitoring and prediction system for quality attributes of jujube slices during the drying process was designed to solve the problem of destructive and inconvenient of the traditional quality detection m...The real-time monitoring and prediction system for quality attributes of jujube slices during the drying process was designed to solve the problem of destructive and inconvenient of the traditional quality detection method and realize quality online monitoring.Firstly,machine vision and automatic weighing were employed to monitor the color and moisture content changes of jujube slices in real-time.Secondly,correlation models between color parameter(a^(*)value)and nutritional quality attributes(vitamin C,reducing sugar)were established to predict vitamin C and reducing sugar content of jujube slices during the drying process.Finally,the upper computer monitoring software was integrated and designed based on LABVIEW virtual instrument,and the real-time monitoring system was tested and validated.Results showed that:the changing trends of color(L^(*),a^(*),and b^(*)values)monitored by the system were basically the same as the results detected by color difference meter,and the average errors of L^(*),a^(*),and b^(*)values were 0.93,0.52,and 0.73,respectively.The average relative error of moisture content between the system monitoring and manual static detection was 0.18%.The average error of vitamin C and reducing sugar content between the system prediction and manual detection were 50 mg/100 g in dry basis and 0.71g/100 g in dry basis,respectively.The current work can provide a useful reference for real-time monitoring of quality attributes of fruits and vegetables during the drying process.展开更多
Biomaterial based scaffolds for treating large bone defects require excellent biocompatibility and osteoconductivity.Here we report on the fabrication of hydroxyapatite-dendritic mesoporous silica nanoparticles(HA-DMS...Biomaterial based scaffolds for treating large bone defects require excellent biocompatibility and osteoconductivity.Here we report on the fabrication of hydroxyapatite-dendritic mesoporous silica nanoparticles(HA-DMSN)based scaffolds with hierarchical micro-pores(5µm)and nano-pores(6.4 nm),and their application for bone regeneration.The in vitro studies demonstrated good biocompatibility of dissolution extracts,as well as enhanced osteogenic potential indicated by dose-dependent upregulation of bone marker gene expression(osteocalcin gene(OCN),osteopontin gene(OPN),collagen type I alpha 1 gene(CoL1A1),runt-related transcription factor 2 gene(RUNX2),and integrin-binding sialoprotein gene(IBSP)),alkaline phosphatise(ALP)activity,and alizarin red staining.The in vivo studies showed that HA-DMSN scaffolds significantly increased bone formation in a rat cranial bone defect model after 4 weeks healing.Our study provides a simple method to fabricate promising inorganic scaffolds with hierarchical pores for bone tissue engineering.展开更多
基金The authors acknowledge the support from UQ Early Career Researcher Grant(1717673)the National Natural Science Foundation of China(Nos.81871503 and 81701032)+2 种基金C.X.acknowledges the support of National Health&Medical Research Council of Australia(NHMRC)Early Career FellowshipY.H.,L.X.and C.L.extended their appreciations to the support of Advanced QueenslandThe authors acknowledge the support from the Australian Microscopy and Microanalysis Research Facility at the Centre for Microscopy and Microanalysis,the University of Queensland.
文摘Biomaterials with suitable osteoimmunomodulation properties and ability to deliver osteoinductive biomolecules,such as bone morphogenetic proteins,are desired for bone regeneration.Herein,we report the development of mesoporous silica rods with large cone-shaped pores(MSR-CP)to load and deliver large protein drugs.It is noted that those cone-shaped pores on the surface modulated the immune response and reduced the pro-inflammatory reaction of stimulated macrophage.Furthermore,bone morphogenetic proteins 2(BMP-2)loaded MSR-CP facilitated osteogenic differentiation and promoted osteogenesis of bone marrow stromal cells.In vivo tests confirmed BMP-2 loaded MSR-CP improved the bone regeneration performance.This study provides a potential strategy for the design of drug delivery systems for bone regeneration.
基金This work was financially supported by the Natural Science Fund of China(Grant No.31960488)the Shihezi University Achievement Transformation and Technology Promotion Project(Grant No.CGZH201808).
文摘The real-time monitoring and prediction system for quality attributes of jujube slices during the drying process was designed to solve the problem of destructive and inconvenient of the traditional quality detection method and realize quality online monitoring.Firstly,machine vision and automatic weighing were employed to monitor the color and moisture content changes of jujube slices in real-time.Secondly,correlation models between color parameter(a^(*)value)and nutritional quality attributes(vitamin C,reducing sugar)were established to predict vitamin C and reducing sugar content of jujube slices during the drying process.Finally,the upper computer monitoring software was integrated and designed based on LABVIEW virtual instrument,and the real-time monitoring system was tested and validated.Results showed that:the changing trends of color(L^(*),a^(*),and b^(*)values)monitored by the system were basically the same as the results detected by color difference meter,and the average errors of L^(*),a^(*),and b^(*)values were 0.93,0.52,and 0.73,respectively.The average relative error of moisture content between the system monitoring and manual static detection was 0.18%.The average error of vitamin C and reducing sugar content between the system prediction and manual detection were 50 mg/100 g in dry basis and 0.71g/100 g in dry basis,respectively.The current work can provide a useful reference for real-time monitoring of quality attributes of fruits and vegetables during the drying process.
基金the support from University of Queensland (UQ) Early Career Researcher Grant (No.1717673).
文摘Biomaterial based scaffolds for treating large bone defects require excellent biocompatibility and osteoconductivity.Here we report on the fabrication of hydroxyapatite-dendritic mesoporous silica nanoparticles(HA-DMSN)based scaffolds with hierarchical micro-pores(5µm)and nano-pores(6.4 nm),and their application for bone regeneration.The in vitro studies demonstrated good biocompatibility of dissolution extracts,as well as enhanced osteogenic potential indicated by dose-dependent upregulation of bone marker gene expression(osteocalcin gene(OCN),osteopontin gene(OPN),collagen type I alpha 1 gene(CoL1A1),runt-related transcription factor 2 gene(RUNX2),and integrin-binding sialoprotein gene(IBSP)),alkaline phosphatise(ALP)activity,and alizarin red staining.The in vivo studies showed that HA-DMSN scaffolds significantly increased bone formation in a rat cranial bone defect model after 4 weeks healing.Our study provides a simple method to fabricate promising inorganic scaffolds with hierarchical pores for bone tissue engineering.
