In this study, rabbit models of optic nerve injury were reproduced by the clamp method. After modeling, rabbit models were given one injection of 50 ng recombinant human ciliary neurotrophic factor into the vitreous b...In this study, rabbit models of optic nerve injury were reproduced by the clamp method. After modeling, rabbit models were given one injection of 50 ng recombinant human ciliary neurotrophic factor into the vitreous body and/or intragastric injection of 4 g/kg compound light granules containing Radix Angelicae Sinensis and Raidix Paeoniae Alba at 4 days after modeling, once per day for 30 consecutive days. After administration, the animals were sacrificed and the intraorbital optic nerve was harvested. Hematoxylin-eosin staining revealed that the injured optic nerve was thinner and optic nerve fibers were irregular. After treatment with recombinant human ciliary neurotrophic factor, the arrangement of optic nerve fibers was disordered but they were not markedly thinner. After treatment with compound light granules, the arrangement of optic nerve fibers was slightly disordered and their structure was intact. After combined treatment with recombinant human ciliary neurotrophic factor and compound light granules, the arrangement of optic nerve fibers was slightly disordered and the degree of injury was less than after either treatment alone. Results of tensile mechanical testing of the optic nerve showed that the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly lower than the normal optic nerve. After treatment with recombinant human ciliary neurotrophic factor and/or compound light granules, the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly increased, especially after the combined treatment. These experimental findings indicate that compound light granules and ciliary neurotrophic factor can alleviate optic nerve injury at the histological and biochemical levels, and the combined treatment is more effective than either treatment alone.展开更多
t The aim of this study was to investigate the feasibility of detecting potassium sorbate(PS)and sorbic acid(SA)in agricultural products using THz time-domain spectroscopy(THz-TDS).The absorption spectra of PS and SA ...t The aim of this study was to investigate the feasibility of detecting potassium sorbate(PS)and sorbic acid(SA)in agricultural products using THz time-domain spectroscopy(THz-TDS).The absorption spectra of PS and SA were measured from 0.2 to 1.6 THz at room temperature.The main characteristic absorption peaks of PS and SA in polyethylene and powdered agricultural products with different weight ratios were detected and analyzed.Interval partial least squares(iPLS)combined with a particle swarm optimization and support vector classification(PSO-SVC)algorithm was proposed in this paper.iPLS was used for frequency optimization,and the PSO-SVC algorithm was used for spectrum analysis of the preservative content based on the optimal spectrum ranges.Optimized PSO-SVC models were obtained when the THz spectrum from the PS/SA mixture was divided into 11 or 12 subintervals.The optimal penalty parameter c and kernel parameter g were found to be 1.284 and 0.863 for PS(0.551-1.487 THz),1.374 and 0.906 for SA(0.454-1.216 THz),respectively.The preliminary results indicate that THz-TDS can be an effective nondestructive analytical tool used for the quantitative detection of additives in agricultural products.展开更多
Following granulocyte colony-stimulating factor （G-CSF） treatment,the growth of processes in cul-tured rat retinal ganglion cells （RGCs） in vitro,expression of growth associated protein 43,and expression of microt...Following granulocyte colony-stimulating factor （G-CSF） treatment,the growth of processes in cul-tured rat retinal ganglion cells （RGCs） in vitro,expression of growth associated protein 43,and expression of microtubule-associated protein 2 mRNA expression were significantly increased.In contrast,RhoA/Rock protein content was significantly reduced by G-CSF treatment.These results indicate that G-CSF promotes the growth of processes in RGCs and increases the expression of growth-associated protein 43 and microtubule-associated protein 2 mRNA by inhibiting the RhoA/Rock pathway,thereby benefiting axonal repair in RGCs exposed to hypoxia.展开更多
Arbuscular mycorrhizal fungi(AMF)can improve plant tolerance to several abiotic stresses,including heavy metals,drought or salinity exposure.However,the role of AMF in alleviation of soil cadmium(Cd)-induced toxicity ...Arbuscular mycorrhizal fungi(AMF)can improve plant tolerance to several abiotic stresses,including heavy metals,drought or salinity exposure.However,the role of AMF in alleviation of soil cadmium(Cd)-induced toxicity to plants is still largely unknown.In this study,Cd speciation in soil and subcellular distribution of Cd were used to characterize the roles of application AM fungi in the alleviation of Cd toxicity in alfalfa plants.Our results showed that the addition of Glomus mosseae in Cd contaminated soil(10 mg/Kg)significantly increased soil pH,cation exchange capacity(CEC)and organic matter in rhizosphere soil with Medicago truncatula L.,and then account for significantly decreased contents of exchangeable and carbonate-bounded Cd speciation in rhizosphere soil,indicating alleviation of plant toxicity by reduction of bioavailable fractions of Cd.Although there is no significant difference found in Cd accumulation by roots and shoots respectively between Cd and AM-Cd treatments,more portion of Cd was recorded compartmentalization in cell wall fraction of both root and shoot in treatment of Cd with AM application,indicating alleviation of Cd toxicity to plant cell.Herein,application of AM fungi in Cd treatments performed to inhibit the appearance of Cd toxicity symptoms,including the improvement of leaf electrolyte leakage,root elongation,seedling growth and biomass.This information provides a clearer understanding of detoxification strategy of AM fungi on Cd behavior with development and stabilization of soil structure and subcellular distribution of plant.展开更多
Wheat stripe rust caused by Puccinia striiformis f.sp.tritici occurs widely in China and seriously affects wheat production.Global warming could profoundly impact the incidence and prevalence of lowtemperature disease...Wheat stripe rust caused by Puccinia striiformis f.sp.tritici occurs widely in China and seriously affects wheat production.Global warming could profoundly impact the incidence and prevalence of lowtemperature diseases such as stripe rust.Studies on the effects of temperature on the distribution of overwintering stripe rust could help us understand the incidence and prevalence of the disease and could also provide support for monitoring,forecasting and developing control strategies.An exponential model and a spherical model of the ordinary Kriging method in the ArcGIS platform were used to predict the overwintering regions of stripe rust based on the probability that the average temperature of the coldest month from December to February was higher than–6 or–7°C from 2001 to 2012.The results showed that the areas with a probability between 70%and 90%were transition regions for the overwintering of stripe rust.Based on annual mean temperature of the coldest month from December to February for 2001 to 2012,overwintering distribution of stripe rust was likewise evaluated.The boundary for overwintering of stripe rust was consistent with the areas where the probability was predicted to be 70%to 90%for the overwintering distribution of stripe rust,but the boundary was shifted northward toward Beijing in North China.Some areas in Xinjiang,including Akto,Pishan,Hotan and Yutian,were also predicted to be suitable for the overwintering of stripe rust.展开更多
Cross-modal selective attention enhances the processing of sensory inputs that are most relevant to the task at hand.Such differential processing could be mediated by a swift network reconfiguration on the macroscopic...Cross-modal selective attention enhances the processing of sensory inputs that are most relevant to the task at hand.Such differential processing could be mediated by a swift network reconfiguration on the macroscopic level,but this remains a poorly understood process.To tackle this issue,we used a behavioral paradigm to introduce a shift of selective attention between the visual and auditory domains,and recorded scalp electroencephalographic signals from eight healthy participants.The changes in effective connectivity caused by the cross-modal attentional shift were delineated by analyzing spectral Granger Causality(GC),a metric of frequency-specific effective connectivity.Using data-driven methods of pattern-classification and feature-analysis,we found that a change in the a band(12 Hz-15 Hz) of GC is a stable feature across different individuals that can be used to decode the attentional shift.Specifically,auditory attention induces more pronounced information flow in the α band,especially from the parietal-occipital areas to the temporal-parietal areas,compared to the case of visual attention,reflecting a reconfiguration of interaction in the macroscopic brain network accompanying different processing.Our results support the role of α oscillation in organizing the information flow across spatially-separated brain areas and,thereby,mediating cross-modal selective attention.展开更多
文摘In this study, rabbit models of optic nerve injury were reproduced by the clamp method. After modeling, rabbit models were given one injection of 50 ng recombinant human ciliary neurotrophic factor into the vitreous body and/or intragastric injection of 4 g/kg compound light granules containing Radix Angelicae Sinensis and Raidix Paeoniae Alba at 4 days after modeling, once per day for 30 consecutive days. After administration, the animals were sacrificed and the intraorbital optic nerve was harvested. Hematoxylin-eosin staining revealed that the injured optic nerve was thinner and optic nerve fibers were irregular. After treatment with recombinant human ciliary neurotrophic factor, the arrangement of optic nerve fibers was disordered but they were not markedly thinner. After treatment with compound light granules, the arrangement of optic nerve fibers was slightly disordered and their structure was intact. After combined treatment with recombinant human ciliary neurotrophic factor and compound light granules, the arrangement of optic nerve fibers was slightly disordered and the degree of injury was less than after either treatment alone. Results of tensile mechanical testing of the optic nerve showed that the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly lower than the normal optic nerve. After treatment with recombinant human ciliary neurotrophic factor and/or compound light granules, the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly increased, especially after the combined treatment. These experimental findings indicate that compound light granules and ciliary neurotrophic factor can alleviate optic nerve injury at the histological and biochemical levels, and the combined treatment is more effective than either treatment alone.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61705061 and 61975053)Key Science and Technology Program of Henan Province of China(Grant Nos.182102110204 and 192102110047)+1 种基金Key Scientific and Research Project of Educational Committee of Henan Province of China(Grant No.19B510001)Open Fund Project of Key Laboratory of Grain Information Processing&Control,Ministry of Education,Henan University of Technology(Grant Nos.KFJJ2016108 and KFJJ2017107).
文摘t The aim of this study was to investigate the feasibility of detecting potassium sorbate(PS)and sorbic acid(SA)in agricultural products using THz time-domain spectroscopy(THz-TDS).The absorption spectra of PS and SA were measured from 0.2 to 1.6 THz at room temperature.The main characteristic absorption peaks of PS and SA in polyethylene and powdered agricultural products with different weight ratios were detected and analyzed.Interval partial least squares(iPLS)combined with a particle swarm optimization and support vector classification(PSO-SVC)algorithm was proposed in this paper.iPLS was used for frequency optimization,and the PSO-SVC algorithm was used for spectrum analysis of the preservative content based on the optimal spectrum ranges.Optimized PSO-SVC models were obtained when the THz spectrum from the PS/SA mixture was divided into 11 or 12 subintervals.The optimal penalty parameter c and kernel parameter g were found to be 1.284 and 0.863 for PS(0.551-1.487 THz),1.374 and 0.906 for SA(0.454-1.216 THz),respectively.The preliminary results indicate that THz-TDS can be an effective nondestructive analytical tool used for the quantitative detection of additives in agricultural products.
文摘Following granulocyte colony-stimulating factor （G-CSF） treatment,the growth of processes in cul-tured rat retinal ganglion cells （RGCs） in vitro,expression of growth associated protein 43,and expression of microtubule-associated protein 2 mRNA expression were significantly increased.In contrast,RhoA/Rock protein content was significantly reduced by G-CSF treatment.These results indicate that G-CSF promotes the growth of processes in RGCs and increases the expression of growth-associated protein 43 and microtubule-associated protein 2 mRNA by inhibiting the RhoA/Rock pathway,thereby benefiting axonal repair in RGCs exposed to hypoxia.
基金This work was financially supported by Grants of Science and Technology Department of Sichuan Province(2019YFS0469,2020YFS0344)Educational Department of Sichuan province(17ZB0438).
文摘Arbuscular mycorrhizal fungi(AMF)can improve plant tolerance to several abiotic stresses,including heavy metals,drought or salinity exposure.However,the role of AMF in alleviation of soil cadmium(Cd)-induced toxicity to plants is still largely unknown.In this study,Cd speciation in soil and subcellular distribution of Cd were used to characterize the roles of application AM fungi in the alleviation of Cd toxicity in alfalfa plants.Our results showed that the addition of Glomus mosseae in Cd contaminated soil(10 mg/Kg)significantly increased soil pH,cation exchange capacity(CEC)and organic matter in rhizosphere soil with Medicago truncatula L.,and then account for significantly decreased contents of exchangeable and carbonate-bounded Cd speciation in rhizosphere soil,indicating alleviation of plant toxicity by reduction of bioavailable fractions of Cd.Although there is no significant difference found in Cd accumulation by roots and shoots respectively between Cd and AM-Cd treatments,more portion of Cd was recorded compartmentalization in cell wall fraction of both root and shoot in treatment of Cd with AM application,indicating alleviation of Cd toxicity to plant cell.Herein,application of AM fungi in Cd treatments performed to inhibit the appearance of Cd toxicity symptoms,including the improvement of leaf electrolyte leakage,root elongation,seedling growth and biomass.This information provides a clearer understanding of detoxification strategy of AM fungi on Cd behavior with development and stabilization of soil structure and subcellular distribution of plant.
基金This study was supported by the National Natural Science Foundation of China(31101393)the National Key Technologies Research and Development Program(2012BAD19BA04).
文摘Wheat stripe rust caused by Puccinia striiformis f.sp.tritici occurs widely in China and seriously affects wheat production.Global warming could profoundly impact the incidence and prevalence of lowtemperature diseases such as stripe rust.Studies on the effects of temperature on the distribution of overwintering stripe rust could help us understand the incidence and prevalence of the disease and could also provide support for monitoring,forecasting and developing control strategies.An exponential model and a spherical model of the ordinary Kriging method in the ArcGIS platform were used to predict the overwintering regions of stripe rust based on the probability that the average temperature of the coldest month from December to February was higher than–6 or–7°C from 2001 to 2012.The results showed that the areas with a probability between 70%and 90%were transition regions for the overwintering of stripe rust.Based on annual mean temperature of the coldest month from December to February for 2001 to 2012,overwintering distribution of stripe rust was likewise evaluated.The boundary for overwintering of stripe rust was consistent with the areas where the probability was predicted to be 70%to 90%for the overwintering distribution of stripe rust,but the boundary was shifted northward toward Beijing in North China.Some areas in Xinjiang,including Akto,Pishan,Hotan and Yutian,were also predicted to be suitable for the overwintering of stripe rust.
基金supported by the National Key Research and Development Program of China (2017YFA0105203)the Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) (XDB32040200 and XDB32030200)+1 种基金the Key Research Program of Frontier Sciences,CAS (QYZDJ-SSWSMC019)the National Natural Science Foundation of China (81871398,U1636121,and 31571003)。
文摘Cross-modal selective attention enhances the processing of sensory inputs that are most relevant to the task at hand.Such differential processing could be mediated by a swift network reconfiguration on the macroscopic level,but this remains a poorly understood process.To tackle this issue,we used a behavioral paradigm to introduce a shift of selective attention between the visual and auditory domains,and recorded scalp electroencephalographic signals from eight healthy participants.The changes in effective connectivity caused by the cross-modal attentional shift were delineated by analyzing spectral Granger Causality(GC),a metric of frequency-specific effective connectivity.Using data-driven methods of pattern-classification and feature-analysis,we found that a change in the a band(12 Hz-15 Hz) of GC is a stable feature across different individuals that can be used to decode the attentional shift.Specifically,auditory attention induces more pronounced information flow in the α band,especially from the parietal-occipital areas to the temporal-parietal areas,compared to the case of visual attention,reflecting a reconfiguration of interaction in the macroscopic brain network accompanying different processing.Our results support the role of α oscillation in organizing the information flow across spatially-separated brain areas and,thereby,mediating cross-modal selective attention.
