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Effects of Cryoprotective Agents on the Bovine Articular Chondrocyte Viability 被引量:1
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作者 X.Xu z.f.cui 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2002年第2期109-112,共4页
Cryopreservation is the process of choice for long term preservation of cells and tissues. In this study, the effects of cryoprotective agents, dimethyl sulfoxide(DMSO), glycerol and 1,2 propanediol on the bovine art... Cryopreservation is the process of choice for long term preservation of cells and tissues. In this study, the effects of cryoprotective agents, dimethyl sulfoxide(DMSO), glycerol and 1,2 propanediol on the bovine articular chondrocyte viability were examined experimentally. The CPA was added at the concentrations of 0 6, 0 9, 1 2 and 1 5 mol/L and at 4 ℃ and 37 ℃ and removed at 37 ℃ in one step. CPA stepwise addition and removal at 0 6 and 1 2 mol/L and at 37 ℃ was also tested as an alternative protocol. Cell volume excursion during DMSO addition and removal was estimated and correlated well with cell survival rates. Solution makeup affects cell survival rate and a stepwise protocol can improve the cell survival rates significantly. 展开更多
关键词 Cryoprotective agent CHONDROCYTE Cell viability CARTILAGE
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Effective cellular vaccines generated by in vitro or in vivo modification of tumor cells using gene transfer approaches for cancer immunogene therapy
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作者 Y.J.Guo F.Shen +16 位作者 T.P.Xie X.Che z.f.cui L.Shi J.Ma S.G.Wu X.N.Wang G.L.Liu Y.Liu H.Wang H.L.Huang L.X.Wei J.Zhao J.Trojan A.Ly D.Anthony M.C.Wu 《中国实验血液学杂志》 CAS CSCD 1997年第3期284-285,共2页
Tumor cells escape host immune surveillance bydown-regulation of MHC and/or co-stimulatorymolecules.Anti-tumor immune responses are mediated primarily by T cells.A deficiency in either MHC or co-stimulatory molecules ... Tumor cells escape host immune surveillance bydown-regulation of MHC and/or co-stimulatorymolecules.Anti-tumor immune responses are mediated primarily by T cells.A deficiency in either MHC or co-stimulatory molecules on tumor cells is associated with a failure to induce anti-tumor immunity. 展开更多
关键词 vaccines surveillance immunity TRANSFECTION recurrence MANNER ESCAPE CURATIVE elicit inducing
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