Lysophosphatidic acid(LPA)is a small molecule glycerophospholipid,which regulates multiple downstream signalling pathways through G-protein-coupled receptors to achieve numerous functions on oocyte maturation and embr...Lysophosphatidic acid(LPA)is a small molecule glycerophospholipid,which regulates multiple downstream signalling pathways through G-protein-coupled receptors to achieve numerous functions on oocyte maturation and embryo development.In this study,sheep in vitro fertilized embryos were applied to investigate the effects of LPA on early embryos development and embryonic stem cell establishment.At first,the maturation medium containing estrus female sheep serum and synthetic oviduct fluid(SOF)were optimized for sheep IVF,and then the effects of LPA were investigated.From 0.1 to 10μmol L^(–1),LPA had no significant effect on the cleavage rate(P>0.05),but the maturation rate and blastocyst rate increased dependently with LPA concentration(P<0.05),and the blastocyst morphology was normal.When the LPA concentration was 15μmol L^(–1),the maturation rate,cleavage rate and blastocyst rate decreased significantly(P<0.05),and the blastocyst exhibited abnormal morphology and could not develop into highquality blastocyst.Besides,the exogenous LPA increases the expression of LPAR2,LPAR4,TE-related gene CDX-2and pluripotency-related gene OCT-4 in sheep early IVF embryos with the raise of LPA concentration from 0.1 to 10μmol L^(–1).The expression of LPAR2,LPAR4,CDX-2 and OCT-4 from the LPA-0.1μmol L^(–1)to LPA-10μmol L^(–1)groups in early embryos were extremely significant(P<0.05),while the expression of these genes significantly decreased in 15μmol L^(–1)LPA-treated embryos compared with LPA-10μmol L^(–1)group(P<0.05).The inner cell mass in 15μmol L^(–1)LPA-treated embryos was also disturbed,and the blastocysts formation was abnormal.Secondly,the sheep IVF blastocysts were applied to establish embryonic stem cells.The results showed that LPA made the blastocyst inoculated cells grow towards TSC-like cells.They enhanced the fluorescence intensity and mRNA abundance of OCT-4 and CDX-2 as the concentration increased from 0 to 10μmol L^(–1),while 15μmol L^(–1)LPA decreased OCT-4 and CDX-2 expression in the derived cells.The expression of CDX-2 and OCT-4 in the blastocyst inoculated cells of LPA-1μmol L^(–1)group and LPA-10μmol L^(–1)group extremely significantly increased(P<0.05),but there was significant decrease in LPA-15μmol L^(–1)group compared with LPA-10μmol L^(–1)group(P<0.05).Meanwhile,the protein expression of LPAR2 and LPAR4 remarkably increased after treatment of LPA at 10μmol L^(–1)concentration.This study references the IVF embryo production and embryonic stem cell research of domestic animals.展开更多
法国DEMETER(Detection of Electro-Magnetic Emissions Transmitted from Earthquake Regions)卫星是世界上第一颗专门针对地震电离层扰动监测的电磁卫星,于2004年发射,2010年底结束运行,共在轨飞行6.5年,获得3万多条整轨数据,为地震...法国DEMETER(Detection of Electro-Magnetic Emissions Transmitted from Earthquake Regions)卫星是世界上第一颗专门针对地震电离层扰动监测的电磁卫星,于2004年发射,2010年底结束运行,共在轨飞行6.5年,获得3万多条整轨数据,为地震监测及电离层物理研究提供了坚实的数据基础。全球科学家利用这颗卫星的数据开展了大量的地震应用研究,发表文章上百篇,将电磁卫星的地震应用研究推向了新的阶段。中国第一颗电磁监测试验卫星CSES(China Seismo-Electromagnetic Satellite)于2018年2月2日成功发射,目前已完成在轨测试并交付使用,科学数据也已正式对外发布。如何充分发挥CSES卫星的应用效能,将空间电磁监测向业务化运行转化,总结和思考DEMETER卫星的技术进步、研究成果及曾经出现过的问题,十分重要。因此,本文着重介绍DEMETER卫星在地震监测应用领域的代表性研究进展,并结合CSES卫星的设计和运转实践,对未来空间地震电磁探测及科学研究的发展方向进行一些初步讨论。展开更多
目的:总结和对比去分支杂交手术和传统手术治疗胸腹主动脉瘤的近远期结果。方法:回顾性分析2008年1月至2019年12月采用传统手术治疗胸腹主动脉瘤的18例患者和采用杂交手术治疗的11例患者的临床资料。杂交手术组中,夹层动脉瘤、术前合并...目的:总结和对比去分支杂交手术和传统手术治疗胸腹主动脉瘤的近远期结果。方法:回顾性分析2008年1月至2019年12月采用传统手术治疗胸腹主动脉瘤的18例患者和采用杂交手术治疗的11例患者的临床资料。杂交手术组中,夹层动脉瘤、术前合并肾功能不全以及美国麻醉医师协会(American Society of Anesthesiologists,ASA)评分≥3分的患者比例明显高于传统手术组。结果:去分支杂交手术患者的手术时间为(445±85)min,术中出血量为(955±599)mL,术后30 d发生并发症2例,没有截瘫,死亡1例,术后30 d并发症发生率为18.2%,死亡率为9.1%。传统手术患者的手术时间为(560±245)min,术中出血量为(6100±4536)mL,术后30 d发生并发症12例,其中截瘫1例,死亡4例,术后30 d并发症发生率为66.7%,死亡率为22.2%。去分支杂交手术的术中出血量明显低于传统手术(P<0.001),30 d内并发症发生率显著降低(P=0.011)。随访期内,杂交手术组二次手术4例,死亡3例,1年、5年和10年的全因生存率分别为72%、54%和29%;传统手术组二次手术1例,死亡5例,1年、5年和10年的全因生存率分别为81%、71%和35%。杂交手术和传统手术在全因生存率和动脉瘤生存率上差异没有统计学意义。结论:去分支杂交手术是一种治疗高危胸腹主动脉瘤患者安全、有效的手术方式,术后早期并发症发生率明显低于传统手术,但中远期疗效仍需要进一步提高。展开更多
基金financially supported by the Science and Technology Major Project of the Inner Mongolia Autonomous Region of China(2020ZD0007)the Major Program of the Inner Mongolia Natural Science Foundation,China(2020ZD10)+3 种基金the National Natural Science Foundation of China(32160172)the Natural Science Foundation of Inner Mongolia Autonomous Region(2020BS03003 and 2020BS03022)the National Transgenic Project of China(2016ZX0801000-002 and 2016ZX08010005-001)the Science and Technology Major Project of the Inner Mongolia Autonomous Region of China(zdzx2018065)。
文摘Lysophosphatidic acid(LPA)is a small molecule glycerophospholipid,which regulates multiple downstream signalling pathways through G-protein-coupled receptors to achieve numerous functions on oocyte maturation and embryo development.In this study,sheep in vitro fertilized embryos were applied to investigate the effects of LPA on early embryos development and embryonic stem cell establishment.At first,the maturation medium containing estrus female sheep serum and synthetic oviduct fluid(SOF)were optimized for sheep IVF,and then the effects of LPA were investigated.From 0.1 to 10μmol L^(–1),LPA had no significant effect on the cleavage rate(P>0.05),but the maturation rate and blastocyst rate increased dependently with LPA concentration(P<0.05),and the blastocyst morphology was normal.When the LPA concentration was 15μmol L^(–1),the maturation rate,cleavage rate and blastocyst rate decreased significantly(P<0.05),and the blastocyst exhibited abnormal morphology and could not develop into highquality blastocyst.Besides,the exogenous LPA increases the expression of LPAR2,LPAR4,TE-related gene CDX-2and pluripotency-related gene OCT-4 in sheep early IVF embryos with the raise of LPA concentration from 0.1 to 10μmol L^(–1).The expression of LPAR2,LPAR4,CDX-2 and OCT-4 from the LPA-0.1μmol L^(–1)to LPA-10μmol L^(–1)groups in early embryos were extremely significant(P<0.05),while the expression of these genes significantly decreased in 15μmol L^(–1)LPA-treated embryos compared with LPA-10μmol L^(–1)group(P<0.05).The inner cell mass in 15μmol L^(–1)LPA-treated embryos was also disturbed,and the blastocysts formation was abnormal.Secondly,the sheep IVF blastocysts were applied to establish embryonic stem cells.The results showed that LPA made the blastocyst inoculated cells grow towards TSC-like cells.They enhanced the fluorescence intensity and mRNA abundance of OCT-4 and CDX-2 as the concentration increased from 0 to 10μmol L^(–1),while 15μmol L^(–1)LPA decreased OCT-4 and CDX-2 expression in the derived cells.The expression of CDX-2 and OCT-4 in the blastocyst inoculated cells of LPA-1μmol L^(–1)group and LPA-10μmol L^(–1)group extremely significantly increased(P<0.05),but there was significant decrease in LPA-15μmol L^(–1)group compared with LPA-10μmol L^(–1)group(P<0.05).Meanwhile,the protein expression of LPAR2 and LPAR4 remarkably increased after treatment of LPA at 10μmol L^(–1)concentration.This study references the IVF embryo production and embryonic stem cell research of domestic animals.
文摘法国DEMETER(Detection of Electro-Magnetic Emissions Transmitted from Earthquake Regions)卫星是世界上第一颗专门针对地震电离层扰动监测的电磁卫星,于2004年发射,2010年底结束运行,共在轨飞行6.5年,获得3万多条整轨数据,为地震监测及电离层物理研究提供了坚实的数据基础。全球科学家利用这颗卫星的数据开展了大量的地震应用研究,发表文章上百篇,将电磁卫星的地震应用研究推向了新的阶段。中国第一颗电磁监测试验卫星CSES(China Seismo-Electromagnetic Satellite)于2018年2月2日成功发射,目前已完成在轨测试并交付使用,科学数据也已正式对外发布。如何充分发挥CSES卫星的应用效能,将空间电磁监测向业务化运行转化,总结和思考DEMETER卫星的技术进步、研究成果及曾经出现过的问题,十分重要。因此,本文着重介绍DEMETER卫星在地震监测应用领域的代表性研究进展,并结合CSES卫星的设计和运转实践,对未来空间地震电磁探测及科学研究的发展方向进行一些初步讨论。
文摘目的:总结和对比去分支杂交手术和传统手术治疗胸腹主动脉瘤的近远期结果。方法:回顾性分析2008年1月至2019年12月采用传统手术治疗胸腹主动脉瘤的18例患者和采用杂交手术治疗的11例患者的临床资料。杂交手术组中,夹层动脉瘤、术前合并肾功能不全以及美国麻醉医师协会(American Society of Anesthesiologists,ASA)评分≥3分的患者比例明显高于传统手术组。结果:去分支杂交手术患者的手术时间为(445±85)min,术中出血量为(955±599)mL,术后30 d发生并发症2例,没有截瘫,死亡1例,术后30 d并发症发生率为18.2%,死亡率为9.1%。传统手术患者的手术时间为(560±245)min,术中出血量为(6100±4536)mL,术后30 d发生并发症12例,其中截瘫1例,死亡4例,术后30 d并发症发生率为66.7%,死亡率为22.2%。去分支杂交手术的术中出血量明显低于传统手术(P<0.001),30 d内并发症发生率显著降低(P=0.011)。随访期内,杂交手术组二次手术4例,死亡3例,1年、5年和10年的全因生存率分别为72%、54%和29%;传统手术组二次手术1例,死亡5例,1年、5年和10年的全因生存率分别为81%、71%和35%。杂交手术和传统手术在全因生存率和动脉瘤生存率上差异没有统计学意义。结论:去分支杂交手术是一种治疗高危胸腹主动脉瘤患者安全、有效的手术方式,术后早期并发症发生率明显低于传统手术,但中远期疗效仍需要进一步提高。
